Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody

Journal of Molecular Biology

Volumn 343, Issue 3, 2004, Pages 685-701

  Midelfort, K S ^a   Hernandez, H H ^a   Lippow, S M ^b,c   Tidor, B ^a,c,d   Drennan, C L ^a   Wittrup, K D ^a,b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d USA   (United States)

Author keywords

antibody binding; electrostatics; kinetics; thermodynamics; very high affinity

Indexed keywords

FLUORESCEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN LIGHT CHAIN; IMMUNOGLOBULIN MU CHAIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CALORIMETRY; CRYSTAL STRUCTURE; DISSOCIATION; ELECTRICITY; ENTHALPY; GENE MUTATION; GENETIC ENGINEERING; KINETICS; MOLECULE; MUTANT; OBSERVATION; PRIORITY JOURNAL; PROTEIN DOMAIN; THEORY; WILD TYPE;

EID: 4744369286     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2004.08.019     Document Type: Article

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