Mass Spectrometric Strategies for Identification and Characterization of Carbonylated Peptides and Proteins

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

Volumn , Issue , 2010, Pages 173-197

  Carini, Marina ^a   Orioli, Marica ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

Advanced lipoxidation end products (ALEs); Carbonylated protein characterization in vitro section, conventional mass spectrometric approaches and peptide and protein detection; Carbonylation, irreversible, non enzymatic modification of proteins and change in activity or function; Mass spectrometric strategies, for identification and characterization of carbonylated peptides and proteins; Mass spectrometry strategies for protein oxidative modification identification; Method for immunoaffinity capture of DNPH derivatized HNE and MDA adducted tryptic peptides; Oxidative decomposition of polyunsaturated fatty acids (PUFAs) chain reactions leading to formation of RCS; Precursor ion scanning specific mass spectrometric detection methods, strategy in studying protein modifications; Protein carbonylation, by reactive carbonyl species (RCS) polyunsaturated fatty acid peroxidation; RCS characterization modified proteins in biological matrices

Indexed keywords

EID: 84886492809     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780813814438.ch11     Document Type: Chapter

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