Intact protein analysis for site-directed mutagenesis overexpression products: Plasmid-encoded R67 dihydrofolate reductase

Analytical Biochemistry

Volumn 305, Issue 1, 2002, Pages 68-81

  VerBerkmoes, Nathan C ^a   Strader, Michael B ^b   Smiley, R Derike ^b   Howell, Elizabeth E ^b   Hurst, Gregory B ^a   Hettich, Robert L ^a   Stephenson Jr , James L ^c  

^a OAK RIDGE NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF TENNESSEE   (United States)

^c RTI INTERNATIONAL   (United States)

Author keywords

Dihydrofolate reductase; Ion ion reactions; Quadrupole ion trap mass spectrometry; Recombinant proteins; Site directed mutagenesis

Indexed keywords

CHEMICAL ANALYSIS; DNA; IONS; MASS SPECTROMETRY; MIXTURES; MUTAGENESIS; PURIFICATION;

DIHYDROFOLATE REDUCTASE; ION/ION REACTIONS; QUADRUPOLE ION TRAPS; R67 DIHYDROFOLATE REDUCTASE; RECOMBINANT PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; TANDEM MASS SPECTROMETRY; TANDEM MASS SPECTROMETRY TECHNIQUES;

RECOMBINANT PROTEINS;

DIHYDROFOLATE REDUCTASE;

ARTICLE; MASS SPECTROMETRY; NONHUMAN; PEPTIDE MAPPING; PLASMID; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; TANDEM MASS SPECTROMETRY;

EID: 0036605610     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2002.5636     Document Type: Article

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