DNA Polymerase ε

Sub-Cellular Biochemistry

Volumn 62, Issue , 2012, Pages 237-257

  Hogg, Matthew ^a   Johansson, Erik ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

DNA replication; Leading strand; Pre loading complex; Processivity

Indexed keywords

EUKARYOTA;

DNA DIRECTED DNA POLYMERASE ALPHA; FUNGAL DNA; FUNGAL PROTEIN;

ANIMAL; BIOSYNTHESIS; DNA REPLICATION; ENZYMOLOGY; EUKARYOTIC CELL; GENETICS; HUMAN; METABOLISM; PHYSIOLOGY; REVIEW; YEAST;

ANIMALS; DNA POLYMERASE II; DNA REPLICATION; DNA, FUNGAL; EUKARYOTIC CELLS; FUNGAL PROTEINS; HUMANS; YEASTS;

EID: 84885836039     PISSN: 03060225     EISSN: None     Source Type: Journal    
DOI: 10.1007/978-94-007-4572-8_13     Document Type: Article

References (104)

1. Aksenova A, Volkov K, Maceluch J, Pursell ZF, Rogozin IB, Kunkel TA, Pavlov YI, Johansson E (2010) Mismatch repair-independent increase in spontaneous mutagenesis in yeast lacking non-essential subunits of DNA polymerase ε. PLoS Genet 6: e1001209.
2. Aparicio OM, Weinstein DM, Bell SP (1997) Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase. Cell 91: 59-69.
3. Araki H, Hamatake RK, Johnston LH, Sugino A (1991) DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88: 4601-4605.
4. Araki H, Ropp PA, Johnson AL, Johnston LH, Morrison A, Sugino A (1992) DNA polymerase II, the probable homolog of mammalian DNA polymerase ε, replicates chromosomal DNA in the yeast Saccharomyces cerevisiae. EMBO J 11: 733-740.
5. Aravind L, Koonin EV (1998) Phosphoesterase domains associated with DNA polymerases of diverse origins. Nucleic Acids Res 26: 3746-3752.
6. Arents G, Moudrianakis EN (1993) Topography of the histone octamer surface: repeating structural motifs utilized in the docking of nucleosomal DNA. Proc Natl Acad Sci USA 90: 10489-10493.
7. Asturias FJ, Cheung IK, Sabouri N, Chilkova O, Wepplo D, Johansson E (2006) Structure of Saccharomyces cerevisiae DNA polymerase epsilon by cryo-electron microscopy. Nat Struct Mol Biol 13: 35-43.
8. Bambara RA, Fay PJ, Mallaber LM (1995) Methods of analyzing processivity. Methods Enzymol 262: 270-280.
9. Baxevanis AD, Arents G, Moudrianakis EN, Landsman D (1995) A variety of DNA-binding and multimeric proteins contain the histone fold motif. Nucleic Acids Res 23: 2685-2691.
10. Bermudez VP, MacNeill SA, Tappin I, Hurwitz J (2002) The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase δ. J Biol Chem 277: 36853-36862.
11. Boiteux S, Guillet M (2004) Abasic sites in DNA: repair and biological consequences in Saccharomyces cerevisiae. DNA Repair (Amst) 3: 1-12.
12. Bonnin A, Lazaro JM, Blanco L, Salas M (1999) A single tyrosine prevents insertion of ribonucleotides in the eukaryotic-type phi29 DNA polymerase. J Mol Biol 290: 241-251.
13. Brown JA, Suo Z (2011) Unlocking the sugar "steric gate" of DNA polymerases. Biochemistry 50: 1135-1142.
14. Bubeck D, Reijns MA, Graham SC, Astell KR, Jones EY, Jackson AP (2011) PCNA directs type 2 RNase H activity on DNA replication and repair substrates. Nucleic Acids Res 39: 3652-3666.
15. Budd ME, Campbell JL (1993) DNA polymerases delta and epsilon are required for chromosomal replication in Saccharomyces cerevisiae. Mol Cell Biol 13: 496-505.
16. Burgers PM (1991) Saccharomyces cerevisiae replication factor C. II. Formation and activity of complexes with the proliferating cell nuclear antigen and with DNA polymerases δ and epsilon. J Biol Chem 266: 22698-22706.
17. Burgess SA, Walker ML, Thirumurugan K, Trinick J, Knight PJ (2004) Use of negative stain and single-particle image processing to explore dynamic properties offlexible macromolecules. J Struct Biol 147: 247-258.
18. Chilkova O, Jonsson BH, Johansson E (2003) The quaternary structure of DNA polymerase ε from Saccharomyces cerevisiae. J Biol Chem 278: 14082-14086.
19. Chilkova O, Stenlund P, Isoz I, Stith CM, Grabowski P, Lundstrom EB, Burgers PM, Johansson E (2007) The eukaryotic leading and lagging strand DNA polymerases are loaded onto primerends via separate mechanisms but have comparable processivity in the presence of PCNA. Nucleic Acids Res 35: 6588-6597.
20. Connolly BA (2009) Recognition of deaminated bases by archaeal family-B DNA polymerases. Biochem Soc Trans 37: 65-68.
21. Dang W, Kagalwala MN, Bartholomew B (2007) The Dpb4 subunit of ISW2 is anchored to extranucleosomal DNA. J Biol Chem 282: 19418-19425.
22. Delarue M, Poch O, Tordo N, Moras D, Argos P (1990) An attempt to unify the structure of polymerases. Protein Eng 3: 461-467.
23. Drury LS, Perkins G, Diffley JF (1997) The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast. EMBO J 16: 5966-5976.
24. Dua R, Levy DL, Campbell JL (1998) Role of the putative zincfinger domain of Saccharomyces cerevisiae DNA polymerase ε in DNA replication and the S/M checkpoint pathway. J Biol Chem 273: 30046-30055.
25. Dua R, Levy DL, Campbell JL (1999) Analysis of the essential functions of the C-terminal protein/protein interaction domain of Saccharomyces cerevisiae pol ε and its unexpected ability to support growth in the absence of the DNA polymerase domain. J Biol Chem 274: 22283-22288.
26. Dua R, Levy DL, Li CM, Snow PM, Campbell JL (2002) In vivo reconstitution of Saccharomyces cerevisiae DNA polymerase ε in insect cells. Purification and characterization. J Biol Chem 277: 7889-7896.
27. Ducoux M, Urbach S, Baldacci G, Hubscher U, Koundrioukoff S, Christensen J, Hughes P (2001) Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21 Cip1-like PCNA-binding motif present in the third subunit of human DNA polymerase δ. J Biol Chem 276: 49258-49266.
28. Feng W, D'Urso G (2001) Schizosaccharomyces pombe cells lacking the amino-terminal catalytic domains of DNA polymerase ε are viable but require the DNA damage checkpoint control. Mol Cell Biol 21: 4495-4504.
29. Feng W, Rodriguez-Menocal L, Tolun G, D'Urso G (2003) Schizosacchromyces pombe Dpb2 binds to origin DNA early in S phase and is required for chromosomal DNA replication. Mol Biol Cell 14: 3427-3436.
30. Fogg MJ, Pearl LH, Connolly BA (2002) Structural basis for uracil recognition by archaeal family B DNA polymerases. Nat Struct Biol 9: 922-927.
31. Fortune JM, Pavlov YI, Welch CM, Johansson E, Burgers PM, Kunkel TA (2005) Saccharomyces cerevisiae DNA polymerase δ: highfidelity for base substitutions but lowerfidelity for singleand multi-base deletions. J Biol Chem 280: 29980-29987.
32. Franklin MC, Wang J, Steitz TA (2001) Structure of the replicating complex of a pol α family DNA polymerase. Cell 105: 657-667.
33. Gardner AF, Jack WE (1999) Determinants of nucleotide sugar recognition in an archaeon DNA polymerase. Nucleic Acids Res 27: 2545-2553.
34. Garg P, Stith CM, Sabouri N, Johansson E, Burgers PM (2004) Idling by DNA polymerase δ maintains a ligatable nick during lagging-strand DNA replication. Genes Dev 18: 2764-2773.
35. Gerik KJ, Li X, Pautz A, Burgers PM (1998) Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase δ. J Biol Chem 273: 19747-19755.
36. Hamatake RK, Hasegawa H, Clark AB, Bebenek K, Kunkel TA, Sugino A (1990) Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. J Biol Chem 265: 4072-4083.
37. Harp JM, Hanson BL, Timm DE, Bunick GJ (2000) Asymmetries in the nucleosome core particle at 2. 5 Å resolution. Acta Crystallogr D Biol Crystallogr 56: 1513-1534.
38. Hartlepp KF, Fernandez-Tornero C, Eberharter A, Grune T, Muller CW, Becker PB (2005) The histone fold subunits of Drosophila CHRAC facilitate nucleosome sliding through dynamic DNA interactions. Mol Cell Biol 25: 9886-9896.
39. Jaszczur M, Flis K, Rudzka J, Kraszewska J, Budd ME, Polaczek P, Campbell JL, Jonczyk P, Fijalkowska IJ (2008) Dpb2p, a noncatalytic subunit of DNA polymerase ε, contributes to thefidelity of DNA replication in Saccharomyces cerevisiae. Genetics 178: 633-647.
40. Jaszczur M, Rudzka J, Kraszewska J, Flis K, Polaczek P, Campbell JL, Fijalkowska IJ, Jonczyk P (2009) Defective interaction between Pol2p and Dpb2p, subunits of DNA polymerase ε, contributes to a mutator phenotype in Saccharomyces cerevisiae. Mutat Res 669: 27-35.
41. Jin YH, Obert R, Burgers PM, Kunkel TA, Resnick MA, Gordenin DA (2001) The 3ʹ →5ʹ exonuclease of DNA polymerase δ can substitute for the 5ʹflap endonuclease Rad27/Fen1 in processing Okazaki fragments and preventing genome instability. Proc Natl Acad Sci USA 98: 5122-5127.
42. Johansson E, Garg P, Burgers PM (2004) The Pol32 subunit of DNA polymerase δ contains separable domains for processive replication and proliferating cell nuclear antigen (PCNA) binding. J Biol Chem 279: 1907-1915.
43. Joyce CM (1997) Choosing the right sugar: how polymerases select a nucleotide substrate. Proc Natl Acad Sci U S A 94: 1619-1622.
44. Karthikeyan R, Vonarx EJ, Straffon AF, Simon M, Faye G, Kunz BA (2000) Evidence from mutational specificity studies that yeast DNA polymerases δ and ε replicate different DNA strands at an intracellular replication fork. J Mol Biol 299: 405-419.
45. Kesti T, Flick K, Keranen S, Syvaoja JE, Wittenberg C (1999) DNA polymerase ε catalytic domains are dispensable for DNA replication, DNA repair, and cell viability. Mol Cell 3: 679-685.
46. Kesti T, McDonald WH, Yates JR 3rd, Wittenberg C (2004) Cell cycle-dependent phosphorylation of the DNA polymerase ε subunit, Dpb2, by the Cdc28 cyclin-dependent protein kinase. J Biol Chem 279: 14245-14255.
47. Kokoska RJ, McCulloch SD, Kunkel TA (2003) The efficiency and specificity of apurinic/apyrimidinic site bypass by human DNA polymerase h and Sulfolobus solfataricus Dpo4. J Biol Chem 278: 50537-50545.
48. Komata M, Bando M, Araki H, Shirahige K (2009) The direct binding of Mrc1, a checkpoint mediator, to Mcm6, a replication helicase, is essential for the replication checkpoint against methyl methanesulfonate-induced stress. Mol Cell Biol 29: 5008-5019.
49. Krakoff IH, Brown NC, Reichard P (1968) Inhibition of ribonucleoside diphosphate reductase by hydroxyurea. Cancer Res 28: 1559-1565.
50. Krogan NJ, Cagney G, Yu H, Zhong G, Guo X, Ignatchenko A, Li J, Pu S, Datta N, Tikuisis AP, Punna T, Peregrin-Alvarez JM, Shales M, Zhang X, Davey M, Robinson MD, Paccanaro A, Bray JE, Sheung A, Beattie B, Richards DP, Canadien V, Lalev A, Mena F, Wong P, Starostine A, Canete MM, Vlasblom J, Wu S, Orsi C, Collins SR, Chandran S, Haw R, Rilstone JJ, Gandi K, Thompson NJ, Musso G, St Onge P, Ghanny S, Lam MH, Butland G, Altaf-Ul AM, Kanaya S, Shilatifard A, O'Shea E, Weissman JS, Ingles CJ, Hughes TR, Parkinson J, Gerstein M, Wodak SJ, Emili A, Greenblatt JF (2006) Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature 440: 637-643.
51. Kunkel TA, Hamatake RK, Motto-Fox J, Fitzgerald MP, Sugino A (1989) Fidelity of DNA polymerase I and the DNA polymerase I-DNA primase complex from Saccharomyces cerevisiae. Mol Cell Biol 9: 4447-4458.
52. Kunkel TA, Roberts JD, Sugino A (1991) Thefidelity of DNA synthesis by the catalytic subunit of yeast DNA polymerase α alone and with accessory proteins. Mutat Res 250: 175-182.
53. Larrea AA, Lujan SA, Nick McElhinny SA, Mieczkowski PA, Resnick MA, Gordenin DA, Kunkel TA (2010) Genome-wide model for the normal eukaryotic DNA replication fork. Proc Natl Acad Sci U S A 107: 17674-17679.
54. Lee SH, Pan ZQ, Kwong AD, Burgers PM, Hurwitz J (1991) Synthesis of DNA by DNA polymerase ε in vitro. J Biol Chem 266: 22707-22717.
55. Lengauer C, Kinzler KW, Vogelstein B (1998) Genetic instabilities in human cancers. Nature 396: 643-649.
56. Levin DS, Bai W, Yao N, O'Donnell M, Tomkinson AE (1997) An interaction between DNA ligase I and proliferating cell nuclear antigen: implications for Okazaki fragment synthesis and joining. Proc Natl Acad Sci U S A 94: 12863-12868.
57. Lou H, Komata M, Katou Y, Guan Z, Reis CC, Budd M, Shirahige K, Campbell JL (2008) Mrc1 and DNA polymerase ε function together in linking DNA replication and the S phase checkpoint. Mol Cell 32: 106-117.
58. McCulloch SD, Kunkel TA (2008) Thefidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases. Cell Res 18: 148-161.
59. McCulloch SD, Kokoska RJ, Chilkova O, Welch CM, Johansson E, Burgers PM, Kunkel TA (2004) Enzymatic switching for efficient and accurate translesion DNA replication. Nucleic Acids Res 32: 4665-4675.
60. Minnick DT, Bebenek K, Osheroff WP, Turner RM Jr, Astatke M, Liu L, Kunkel TA, Joyce CM (1999) Side chains that influencefidelity at the polymerase active site of Escherichia coli DNA polymerase I (Klenow fragment). J Biol Chem 274: 3067-3075.
61. Minnick DT, Liu L, Grindley ND, Kunkel TA, Joyce CM (2002) Discrimination against purinepyrimidine mispairs in the polymerase active site of DNA polymerase I: a structural explanation. Proc Natl Acad Sci USA 99: 1194-1199.
62. Mirkin EV, Mirkin SM (2007) Replication fork stalling at natural impediments. Microbiol Mol Biol Rev 71: 13-35.
63. Morrison A, Bell JB, Kunkel TA, Sugino A (1991) Eukaryotic DNA polymerase amino acid sequence required for 3ʹ →5ʹ exonuclease activity. Proc Natl Acad Sci U S A 88: 9473-9477.
64. Morrison A, Johnson AL, Johnston LH, Sugino A (1993) Pathway correcting DNA replication errors in Saccharomyces cerevisiae. EMBO J 12: 1467-1473.
65. Muramatsu S, Hirai K, Tak YS, Kamimura Y, Araki H (2010) CDK-dependent complex formation between replication proteins Dpb11, Sld2, Pol ε, and GINS in budding yeast. Genes Dev 24: 602-612.
66. Navadgi-Patil VM, Burgers PM (2009) A tale of two tails: activation of DNA damage checkpoint kinase Mec1/ATR by the 9-1-1 clamp and by Dpb11/TopBP1. DNA Repair (Amst) 8: 996-1003.
67. Navas TA, Zhou Z, Elledge SJ (1995) DNA polymerase ε links the DNA replication machinery to the S phase checkpoint. Cell 80: 29-39.
68. Navas TA, Sanchez Y, Elledge SJ (1996) RAD9 and DNA polymerase ε form parallel sensory branches for transducing the DNA damage checkpoint signal in Saccharomyces cerevisiae. Genes Dev 10: 2632-2643.
69. Nick McElhinny SA, Gordenin DA, Stith CM, Burgers PM, Kunkel TA (2008) Division of labor at the eukaryotic replication fork. Mol Cell 30: 137-144.
70. Nick McElhinny SA, Kumar D, Clark AB, Watt DL, Watts BE, Lundstrom EB, Johansson E, Chabes A, Kunkel TA (2010a) Genome instability due to ribonucleotide incorporation into DNA. Nat Chem Biol 6: 774-781.
71. Nick McElhinny SA, Watts BE, Kumar D, Watt DL, Lundstrom EB, Burgers PM, Johansson E, Chabes A, Kunkel TA (2010b) Abundant ribonucleotide incorporation into DNA by yeast replicative polymerases. Proc Natl Acad Sci U S A 107: 4949-4954.
72. Niedernhofer LJ, Lalai AS, Hoeijmakers JH (2005) Fanconi anemia (cross)linked to DNA repair. Cell 123: 1191-1198.
73. Nuutinen T, Tossavainen H, Fredriksson K, Pirila P, Permi P, Pospiech H, Syvaoja JE (2008) The solution structure of the amino-terminal domain of human DNA polymerase ε subunit B is homologous to C-domains of AAA+ proteins. Nucleic Acids Res 36: 5102-5110.
74. Ohya T, Maki S, Kawasaki Y, Sugino A (2000) Structure and function of the fourth subunit (Dpb4p) of DNA polymerase ε in Saccharomyces cerevisiae. Nucleic Acids Res 28: 3846-3852.
75. Ohya T, Kawasaki Y, Hiraga S, Kanbara S, Nakajo K, Nakashima N, Suzuki A, Sugino A (2002) The DNA polymerase domain of pol(epsilon) is required for rapid, efficient, and highly accurate chromosomal DNA replication, telomere length maintenance, and normal cell senescence in Saccharomyces cerevisiae. J Biol Chem 277: 28099-28108.
76. Pavlov YI, Frahm C, Nick McElhinny SA, Niimi A, Suzuki M, Kunkel TA (2006) Evidence that errors made by DNA polymerase α are corrected by DNA polymerase δ. Curr Biol 16: 202-207.
77. Payne BT, van Knippenberg IC, Bell H, Filipe SR, Sherratt DJ, McGlynn P (2006) Replication fork blockage by transcription factor-DNA complexes in Escherichia coli. Nucleic Acids Res 34: 5194-5202.
78. Penczek PA, Grassucci RA, Frank J (1994) The ribosome at improved resolution: new techniques for merging and orientation refinement in 3D cryo-electron microscopy of biological particles. Ultramicroscopy 53: 251-270.
79. Puddu F, Piergiovanni G, Plevani P, Muzi-Falconi M (2011) Sensing of replication stress and Mec1 activation act through two independent pathways involving the 9-1-1 complex and DNA polymerase ε. PLoS Genet 7: e1002022.
80. Pursell ZF, Kunkel TA (2008) DNA polymerase epsilon: a polymerase of unusual size (and complexity). Prog Nucleic Acid Res Mol Biol 82: 101-145.
81. Pursell ZF, Isoz I, Lundstrom EB, Johansson E, Kunkel TA (2007) Yeast DNA polymerase ε participates in leading-strand DNA replication. Science 317: 127-130.
82. Randell JC, Bowers JL, Rodriguez HK, Bell SP (2006) Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicase. Mol Cell 21: 29-39.
83. Rogozin IB, Makarova KS, Pavlov YI, Koonin EV (2008) A highly conserved family of inactivated archaeal B family DNA polymerases. Biol Direct 3: 32.
84. Shamoo Y, Steitz TA (1999) Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex. Cell 99: 155-166.
85. Shcherbakova PV, Pavlov YI (1996) 3ʹ →5ʹ Exonucleases of DNA polymerases ε and δ correct base analog induced DNA replication errors on opposite DNA strands in Saccharomyces cerevisiae. Genetics 142: 717-726.
86. Shcherbakova PV, Bebenek K, Kunkel TA (2003a) Functions of eukaryotic DNA polymerases. Sci Aging Knowledge Environ 2003: RE3.
87. Shcherbakova PV, Pavlov YI, Chilkova O, Rogozin IB, Johansson E, Kunkel TA (2003b) Unique error signature of the four-subunit yeast DNA polymerase ε. J Biol Chem 278: 43770-43780.
88. Shikata K, Sasa-Masuda T, Okuno Y, Waga S, Sugino A (2006) The DNA polymerase activity of Pol ε holoenzyme is required for rapid and efficient chromosomal DNA replication in Xenopus egg extracts. BMC Biochem 7: 21.
89. Shiotani B, Kobayashi M, Watanabe M, Yamamoto K, Sugimura T, Wakabayashi K (2006) Involvement of the ATR-and ATM-dependent checkpoint responses in cell cycle arrest evoked by pierisin-1. Mol Cancer Res 4: 125-133.
90. Steitz TA (1993) DNA-and RNA-dependent DNA polymerases. Curr Opin Struct Biol 3: 31-38.
91. Stinchcomb DT, Struhl K, Davis RW (1979) Isolation and characterisation of a yeast chromosomal replicator. Nature 282: 39-43.
92. Stocki SA, Nonay RL, Reha-Krantz LJ (1995) Dynamics of bacteriophage T4 DNA polymerase function: identification of amino acid residues that affect switching between polymerase and 3ʹ →5ʹ exonuclease activities. J Mol Biol 254: 15-28.
93. Tahirov TH, Makarova KS, Rogozin IB, Pavlov YI, Koonin EV (2009) Evolution of DNA polymerases: an inactivated polymerase-exonuclease module in Pol ε and a chimeric origin of eukaryotic polymerases from two classes of archaeal ancestors. Biol Direct 4: 11.
94. Takayama Y, Kamimura Y, Okawa M, Muramatsu S, Sugino A, Araki H (2003) GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast. Genes Dev 17: 1153-1165.
95. Traut TW (1994) Physiological concentrations of purines and pyrimidines. Mol Cell Biochem 140: 1-22.
96. Tsubota T, Maki S, Kubota H, Sugino A, Maki H (2003) Double-stranded DNA binding properties of Saccharomyces cerevisiae DNA polymerase ε and of the Dpb3p-Dpb4p subassembly. Genes Cells 8: 873-888.
97. Tsubota T, Tajima R, Ode K, Kubota H, Fukuhara N, Kawabata T, Maki S, Maki H (2006) Doublestranded DNA binding, an unusual property of DNA polymerase ε, promotes epigenetic silencing in Saccharomyces cerevisiae. J Biol Chem 281: 32898-32908.
98. Warbrick E, Lane DP, Glover DM, Cox LS (1997) Homologous regions of Fen1 and p21Cip1 compete for binding to the same site on PCNA: a potential mechanism to co-ordinate DNA replication and repair. Oncogene 14: 2313-2321.
99. Warbrick E, Heatherington W, Lane DP, Glover DM (1998) PCNA binding proteins in Drosophila melanogaster: the analysis of a conserved PCNA binding domain. Nucleic Acids Res 26: 3925-3932.
100. Wardle J, Burgers PM, Cann IK, Darley K, Heslop P, Johansson E, Lin LJ, McGlynn P, Sanvoisin J, Stith CM, Connolly BA (2008) Uracil recognition by replicative DNA polymerases is limited to the archaea, not occurring with bacteria and eukarya. Nucleic Acids Res 36: 705-711.
101. Watt DL, Johansson E, Burgers PM, Kunkel TA (2011) Replication of ribonucleotide-containing DNA templates by yeast replicative polymerases. DNA Repair (Amst) 10: 897-902.
102. Wu P, Nossal N, Benkovic SJ (1998) Kinetic characterization of a bacteriophage T4 antimutator DNA polymerase. Biochemistry 37: 14748-14755.
103. Yang G, Lin T, Karam J, Konigsberg WH (1999) Steady-state kinetic characterization of RB69 DNA polymerase mutants that affect dNTP incorporation. Biochemistry 38: 8094-8101.
104. Yang G, Franklin M, Li J, Lin TC, Konigsberg W (2002) A conserved Tyr residue is required for sugar selectivity in a Pol α DNA polymerase. Biochemistry 41: 10256-10261.