Identifying critical unrecognized sugar-protein interactions in GH10 xylanases from Geobacillus stearothermophilus using STD NMR

FEBS Journal

Volumn 280, Issue 18, 2013, Pages 4652-4665

  Balazs, Yael S ^a   Lisitsin, Elina ^a   Carmiel, Oshrat ^a   Shoham, Gil ^b   Shoham, Yuval ^c   Schmidt, Asher ^a  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^b TEVA PHARMACEUTICALS   (Israel)

^c HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

GH10 xylanase; glycosidase; saturation transfer difference; STD NMR; sugar binding

Indexed keywords

DISACCHARIDE; MONOMER; MUTANT PROTEIN; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLOBIOSE; XYLOSE; XYLOTRIOSE;

ARTICLE; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; COVALENT BOND; CRYSTAL STRUCTURE; ENZYME SUBSTRATE COMPLEX; GENETIC CONSERVATION; GEOBACILLUS STEAROTHERMOPHILUS; MOLECULAR INTERACTION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTON NUCLEAR MAGNETIC RESONANCE; SATURATION TRANSFER DIFFERENCE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; WILD TYPE;

GH10 XYLANASE; GLYCOSIDASE; SATURATION TRANSFER DIFFERENCE; STD NMR; SUGAR BINDING;

BACTERIAL PROTEINS; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DISACCHARIDES; ENDO-1,4-BETA XYLANASES; ESCHERICHIA COLI; GEOBACILLUS STEAROTHERMOPHILUS; GLUTAMIC ACID; GLUTAMINE; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; ISOMERISM; KINETICS; MODELS, MOLECULAR; MUTATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; THERMODYNAMICS; XYLOSE;

GEOBACILLUS STEAROTHERMOPHILUS;

EID: 84883457977     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12437     Document Type: Article

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