Thermal-induced conformational changes in the product release area drive the enzymatic activity of xylanases 10B: Crystal structure, conformational stability and functional characterization of the xylanase 10B from Thermotoga petrophila RKU-1

Biochemical and Biophysical Research Communications

Volumn 403, Issue 2, 2010, Pages 214-219

  Santos, Camila Ramos ^a   Meza, Andreia Navarro ^a   Hoffmam, Zaira Bruna ^b   Silva, Junio Cota ^b   Alvarez, Thabata Maria ^b   Ruller, Roberto ^b   Giesel, Guilherme Menegon ^c   Verli, Hugo ^c   Squina, Fabio Marcio ^b   Prade, Rolf Alexander ^d   Murakami, Mario Tyago ^a  

^a BRAZILIAN CENTER FOR RESEARCH IN ENERGY AND MATERIALS CNPEM   (Brazil)

^b BRAZILIAN CENTER FOR RESEARCH IN ENERGY AND MATERIALS CNPEM   (Brazil)

^c FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^d OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

Crystal structure; Glycoside hydrolase family 10; Thermostability; Thermotoga petrophila RKU 1; Xylanase

Indexed keywords

BACTERIAL ENZYME; ENDO 1,4 BETA XYLANASE; UNCLASSIFIED DRUG; XYLANASE 10B; XYLOBIOSE; XYLOTRIOSE;

ARTICLE; BIOCHEMISTRY; BIOPHYSICS; CAPILLARY ZONE ELECTROPHORESIS; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; COUPLING FACTOR; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME MODIFICATION; ENZYME RELEASE; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME STRUCTURE; HIGH TEMPERATURE; HYDROLYSIS; HYPERTHERMOPHILIC BACTERIUM; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; THERMOSTABILITY; THERMOTOGA; THERMOTOGA PETROPHILA;

BACTERIA; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DISACCHARIDES; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; HOT TEMPERATURE; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY;

BACTERIA (MICROORGANISMS); THERMOTOGA PETROPHILA;

EID: 78649907582     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.11.010     Document Type: Article

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