Construction and application of a food-grade expression system for lactococcus lactis

Molecular Biotechnology

Volumn 54, Issue 2, 2013, Pages 170-176

  Lu, Wenwei ^a   Kong, Jian ^a   Kong, Wentao ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Alanine racemase gene; Complementation marker; Food grade expression system; Lactococcus lactis; Live vaccine

Indexed keywords

ALANINE RACEMASE; COMPLEMENTATION; FOOD-GRADE EXPRESSION SYSTEMS; LACTOCOCCUS LACTIS; LIVE VACCINES;

AMINO ACIDS; ANTIBIOTICS; AUTOMOBILE MANUFACTURE;

GENE EXPRESSION;

ALANINE RACEMASE; ANTIGEN; CAPSID PROTEIN; DNA; GREEN FLUORESCENT PROTEIN;

ARTICLE; AUXOTROPHIC MUTANT; CIRCOVIRUS; FOOD; FOOD GRADE EXPRESSION SYSTEM; FOOD INDUSTRY; GENE; GENE EXPRESSION SYSTEM; HOST; INTEGRATION; LACTOBACILLUS CASEI; LACTOCOCCUS LACTIS; NONHUMAN; PLASMID; REPLICON; TEMPERATURE;

ALANINE RACEMASE; BACTERIAL PROTEINS; CAPSID PROTEINS; CIRCOVIRUS; FOOD INDUSTRY; FOOD MICROBIOLOGY; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LACTOBACILLUS CASEI; LACTOCOCCUS LACTIS; MUTATION; NISIN; PLASMIDS; PROMOTER REGIONS, GENETIC;

LACTOBACILLUS CASEI; LACTOCOCCUS LACTIS; MIRIDAE; PORCINE CIRCOVIRUS;

EID: 84882851026     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-012-9558-z     Document Type: Article

References (38)

1. Moeini, H., Rahim, R. A., Omar, A. R., Shafee, N., & Yusoff, K. (2011). Lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus. Applied Microbiology and Biotechnology, 90, 77-88.
2. Drouault, S., Corthier, G., Ehrlich, S. D., & Renault, P. (1999). Survival, physiology, and lysis of Lactococcus lactis in the digestive tract. Applied and Environmental Microbiology, 65, 4881.
3. Kuipers, O. P., de Ruyter, P. G., Kleerebezem, M., & de Vos, W. M. (1997). Controlled overproduction of proteins by lactic acid bacteria. Trends in Biotechnology, 15, 135-140.
4. Mierau, I., & Kleerebezem, M. (2005). 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Applied Microbiology and Biotechnology, 68, 705-717.
5. Bermúdez-Humarán, L. G., Cortes-Perez, N. G., Lefèvre, F., Guimar es, V., Rabot, S., Alcocer-Gonzalez, J. M., et al. (2005). A novel mucosal vaccine based on live Lactococci expressing E7 antigen and IL-12 induces systemic and mucosal immune responses and protects mice against human papillomavirus type 16-induced tumors. The Journal of Immunology, 175, 7297.
6. Ribeiro, L. A., Azevedo, V., Le Loir, Y., Oliveira, S. C., Dieye, Y., Piard, J. C., et al. (2002). Production and targeting of the Brucella abortus antigen L7/L12 in Lactococcus lactis: A first step towards food-grade live vaccines against brucellosis. Applied and Environmental Microbiology, 68, 910.
7. Zhou, J. Y., Shang, S. B., Gong, H., Chen, Q. X., Wu, J. X., Shen, H. G., et al. (2005). In vitro expression, monoclonal antibody and bioactivity for capsid protein of porcine circovirus type II without nuclear localization signal. Journal of Biotechnology, 118, 201-211.
8. Enouf, V., Langella, P., Commissaire, J., Cohen, J., & Corthier, G. (2001). Bovine rotavirus nonstructural protein 4 produced by Lactococcus lactis is antigenic and immunogenic. Applied and Environment Microbiology, 67, 1423-1428.
9. Bermudez-Humaran, L. G., Langella, P., Commissaire, J., Gilbert, S., Le Loir, Y., L'Haridon, R., et al. (2003). Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis. FEMS Microbiology Letters, 224, 307-313.
10. Hazebrouck, S., Pothelune, L., Azevedo, V., Corthier, G., Wal, J. M., & Langella, P. (2007). Efficient production and secretion of bovine beta-lactoglobulin by Lactobacillus casei. Microbial Cell Factories, 6, 12.
11. de Vos, W. M. (1999). Safe and sustainable systems for food-grade fermentations by genetically modified lactic acid bacteria. International Dairy Journal, 9, 3-10.
12. Posno, M., Heuvelmans, P. T., van Giezen, M. J., Lokman, B. C., Leer, R. J., & Pouwels, P. H. (1991). Complementation of the inability of Lactobacillus strains to utilize d-xylose with d-xylose catabolism-encoding genes of Lactobacillus pentosus. Applied and Environment Microbiology, 57, 2764-2766.
13. Gosalbes, M. J., Esteban, C. D., Galan, J. L., & Perez-Martinez, G. (2000). Integrative food-grade expression system based on the lactose regulon of Lactobacillus casei. Applied and Environment Microbiology, 66, 4822-4828.
14. Boucher, I., Parrot, M., Gaudreau, H., Champagne, C. P., Vadeboncoeur, C., & Moineau, S. (2002). Novel food-grade plasmid vector based on melibiose fermentation for the genetic engineering of Lactococcus lactis. Applied and Environment Microbiology, 68, 6152-6161.
15. Takala, T. M., & Saris, P. E. (2002). A food-grade cloning vector for lactic acid bacteria based on the nisin immunity gene nisI. Applied Microbiology and Biotechnology, 59, 467-471.
16. Liu, C. Q., Su, P., Khunajakr, N., Deng, Y. M., Sumual, S., Kim, W. S., et al. (2005). Development of food-grade cloning and expression vectors for Lactococcus lactis. Journal of Applied Microbiology, 98, 127-135.
17. Sorensen, K. I., Larsen, R., Kibenich, A., Junge, M. P., & Johansen, E. (2000). A food-grade cloning system for industrial strains of Lactococcus lactis. Applied and Environment Microbiology, 66, 1253-1258.
18. Sasaki, Y., Ito, Y., & Sasaki, T. (2004). ThyA as a selection marker in construction of food-grade host-vector and integration systems for Streptococcus thermophilus. Applied and Environment Microbiology, 70, 1858-1864.
19. Platteeuw, C., van Alen-Boerrigter, I., van Schalkwijk, S., & de Vos, W. M. (1996). Food-grade cloning and expression system for Lactococcus lactis. Applied and Environment Microbiology, 62, 1008-1013.
20. Takala, T. M., Saris, P. E., & Tynkkynen, S. S. (2003). Food-grade host/vector expression system for Lactobacillus casei based on complementation of plasmid-associated phospho-beta-galactosidase gene lacG. Applied Microbiology and Biotechnology, 60, 564-570.
21. Hols, P., Kleerebezem, M., Schanck, A. N., Ferain, T., Hugenholtz, J., Delcour, J., et al. (1999). Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering. Nature Biotechnology, 17, 588-592.
22. Bron, P. A., Benchimol, M. G., Lambert, J., Palumbo, E., Deghorain, M., Delcour, J., et al. (2002). Use of the alr gene as a food-grade selection marker in lactic acid bacteria. Applied and Environmental Microbiology, 68, 5663.
23. Nguyen, T. T., Mathiesen, G., Fredriksen, L., Kittl, R., Nguyen, T. H., Eijsink, V. G., et al. (2011). A food-grade system for inducible gene expression in Lactobacillus plantarum using an alanine racemase-encoding selection marker. Journal of Agriculture and Food Chemistry, 59, 5617-5624.
24. Rijnen, L., Courtin, P., Gripon, J. C., & Yvon, M. (2000). Expression of a heterologous glutamate dehydrogenase gene in Lactococcus lactis highly improves the conversion of amino acids to aroma compounds. Applied and Environment Microbiology, 66, 1354-1359.
25. Tangney, M., & Fitzgerald, G. F. (2002). AbiA, a lactococcal abortive infection mechanism functioning in Streptococcus thermophilus. Applied and Environment Microbiology, 68, 6388-6391.
26. Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology, 166, 557-580.
27. Kuipers, O. P., de Ruyter, P. G. G. A., Kleerebezem, M., & de Vos, W. M. (1998). Quorum sensing-controlled gene expression in lactic acid bacteria. Journal of Biotechnology, 64, 15-21.
28. de Vos, W. M., Vos, P., de Haard, H., & Boerrigter, I. (1989). Cloning and expression of the Lactococcus lactis subsp. cremoris SK11 gene encoding an extracellular serine proteinase. Gene, 85, 169-176.
29. Ferain, T., Hobbs, J., Jr., Richardson, J., Bernard, N., Garmyn, D., Hols, P., et al. (1996). Knockout of the two ldh genes has a major impact on peptidoglycan precursor synthesis in Lactobacillus plantarum. Journal of Bacteriology, 178, 5431.
30. Holo, H., & Nes, I. F. (1989). High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Applied and Environmental Microbiology, 55, 3119.
31. Maguin, E., Prevost, H., Ehrlich, S. D., & Gruss, A. (1996). Efficient insertional mutagenesis in lactococci and other gram-positive bacteria. Journal of Bacteriology, 178, 931-935.
32. Biswas, I., Gruss, A., Ehrlich, S. D., & Maguin, E. (1993). High-efficiency gene inactivation and replacement system for gram-positive bacteria. Journal of Bacteriology, 175, 3628.
33. Waldo, G. S., Standish, B. M., Berendzen, J., & Terwilliger, T. C. (1999). Rapid protein-folding assay using green fluorescent protein. Nature Biotechnology, 17, 691-695.
34. Wang, K., Huang, L., Kong, J., & Zhang, X. (2008). Expression of the capsid protein of porcine circovirus type 2 in Lactococcus lactis for oral vaccination. Journal of Virological Methods, 150, 1-6.
35. Mori, H., & Ito, K. (2001). The Sec protein-translocation pathway. Trends in Microbiology, 9, 494-500.
36. Martin, M. C., Fernandez, M., Martin-Alonso, J. M., Parra, F., Boga, J. A., & Alvarez, M. A. (2004). Nisin-controlled expression of Norwalk virus VP60 protein in Lactobacillus casei. FEMS Microbiology Letters, 237, 385-391.
37. Le Loir, Y., Azevedo, V., Oliveira, S. C., Freitas, D. A., Miyoshi, A., Bermudez-Humaran, L. G., et al. (2005). Protein secretion in Lactococcus lactis: An efficient way to increase the overall heterologous protein production. Microbial Cell Factories, 4, 2.
38. Beasley, S. S., & Saris, P. E. (2004). Nisin-producing Lactococcus lactis strains isolated from human milk. Applied and Environment Microbiology, 70, 5051-5053.