Conversion of Lactococcus lactis from homolactic to homoalanine fermentation through metabolic engineering

Nature Biotechnology

Volumn 17, Issue 6, 1999, Pages 588-592

  Hols, Pascal ^a,b   Kleerebezem, Michiel ^a   Schanck, André N ^c   Ferain, Thierry ^b   Hugenholtz, Jeroen ^a   Delcour, Jean ^b   De Vos, Willem M ^a  

^a NIZO FOOD RESEARCH   (Netherlands)

^b Lab de Genet Microbienne   (Belgium)

^c UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

Author keywords

Alanine; Alanine dehydrogenase; Alanine racemase; Lactococcus lactis

Indexed keywords

ALANINE; ALANINE DEHYDROGENASE; AMINO ACID; LACTATE DEHYDROGENASE; RACEMASE;

AMINO ACID SEQUENCE; ARTHROBACTER; ARTICLE; BREVIBACTERIUM; CLOSTRIDIUM; CORYNEBACTERIUM; ENZYME ACTIVITY; ENZYME MECHANISM; FERMENTATION; GENE EXPRESSION; LACTOCOCCUS LACTIS; PRIORITY JOURNAL; PYROCOCCUS; STEREOISOMERISM;

ALANINE; ALANINE DEHYDROGENASE; ALANINE RACEMASE; AMINO ACID OXIDOREDUCTASES; BACILLUS; BASE SEQUENCE; CATALYSIS; DNA PRIMERS; FERMENTATION; ISOMERISM; LACTATES; LACTOCOCCUS LACTIS; MOLECULAR SEQUENCE DATA;

EID: 0033031823     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/9902     Document Type: Article

References (40)

1. Leuchtenberger, W. in Products of primary metabolism Vol. 6 (vol. ed. Roehr, M.). Biotechnology (eds Rhem, H.-J., Reed, G., Pühler, A. & Stadler, P.J.W.) 465-502 (VCH Verlagsgesellschaft mbH, Weinheim, Germany; 1996).
2. Hirose, Y. & Okada, H. in Microbial technology, microbial process (eds Peppler, H.J. & Perlman, D.) 211-240 (Academic, New York; 1979).
3. Yamamoto, T., Kato, T., Matsuo, R., Kawamura, Y. & Yoshida, M. Gustatory reaction time to various sweeteners in human adults. Physiol. Behav. 35, 411-415 (1985).
4. Kitai, A. in The microbial production of amino acids (eds Yamada, K., Kinoshita, S., Tsunoda, T. & Aida, K.) 325-337 (Kodansha Ltd, Tokyo, John Wiley and Sons, New York; 1972).
5. Ravot, G. et al. L-alanine production from glucose fermentation by hyperthermophilic members of the domains bacteria and archaea: a remnant of an ancestral metabolism? Appl. Environ. Microbiol. 62, 2657-2659 (1996).
6. Orlygsson, J., Anderson, R. & Svensson, B.H. Alanine as an end product during fermentation of monosaccharides by Clostridium strain P2. Antonie Van Leeuwenhoek 68, 273-280 (1995).
7. Hashimoto, S.-I. & Katsumata, R. Overproduction of alanine by Arthrobacter strains with glucose-nonrepressible L-alanine dehydrogenase. Biotechnol. Lett. 15, 1117-1122 (1993).
8. Furui, M. & Yamashita, K. Pressurized reaction method for continious production of L-alanine by immobilized Pseudomonas dacunhae cells. J. Ferment. Technol. 61, 587-591 (1983).
9. Galkin, A., Kulakova, L., Yoshimura, T., Soda, K. & Esaki, N. Synthesis of optically active amino acids from α-keto acids with Eschericihia coli cells expressing heterologous genes. Appl. Environ. Microbiol. 63, 4651-4656 (1997).
10. Uhlenbusch, I., Sahm, H. & Sprenger, G.A. Expression of the L-alanine dehydrogenasse gene in Zymomonas mobilis and excretion of L-alanine. Appl. Environ. Microbiol 57, 1360-1366 (1991).
11. Eikmanns, B., Sham, H. & Sprenger, G. Metabolic design bei Bakterien: gezielte Entwicklung von aminosäureproduzierenden Bakterien mit Hilfe der Gentechnik Bioscope 2/95, 39-45 (1995).
12. de Vos, W.M. Metabolic engineering of sugar catabolism in lactic acid bacteria. Antonie Van Leeuwenhoek 70, 223-242 (1996).
13. Ohashima, T. & Soda, K. Purification and properties of alanine dehydrogenase from Bacillus sphaericus. Eur. J. Biochem. 100, 29-39 (1979).
14. Hillier, A.J. & Jago, G.R. L-lactate dehydrogenase, FDP-activated, from Streptococcus cremoris. Methods Enzymol. 89, 362-367 (1982).
15. de Ruyter, P.G.G.A., Kuipers, O.P. & de Vos, W.M. Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl. Environ. Microbiol. 62, 3662-3667 (1996).
16. Kuipers, O.P., de Ruyter, P.G.G.A., Kleerebezem, M. & de Vos, W.M. Controlled overproduction of proteins by lactic acid bacteria. Trends Biotechnol. 15, 135-140 (1997).
17. +-dependent dehydrogenases. Biochemistry 29, 1009-1015 (1990).
18. Platteeuw, C., Hugenholtz, J., Starrenburg, M., Alen-Boerrigter, I. & de Vos, W.M. Metabolic engineering of Lactococcus lactis: influence of the overproduction of α-acetolactate synthase in strains deficient in lactate dehydrogenase as a function of culture conditions. Appl. Environ. Microbiol. 61, 3967-3971 (1995).
19. 13C nuclear magnetic resonance analysis of glucose and citrate end products in a ldhL-ldhD double knockout strain of Lactobacillus plantarum. Appl. Environ. Microbiol. 178, 7311-7315 (1996).
20. Foucaud, C., Herve, M., Neumann, J.M. & Hemme, D. 1995. Glucose metabolism and internal pH of Lactococcus lactis subsp. lactis cells utilizing NMR spectroscopy. Lett. Appl. Microbiol. 21, 10-13 (1995).
21. Snoep, J.L., de Mattos, M.J.T., Starrenburg, M.J.C. & Hugenholtz, J. Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and α-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis. J. Bacteriol. 174, 4838-4841 (1992).
22. Mou, L., Sullivan, J.J. & Jago, G.R. Autolysis of Streptococcus cremons. J. Dairy Res. 43, 275-282 (1976).
23. Vegarud, G., Castberg, H.B. & Langsrud, T. Autolysis of group N streptococci. Effects of media composition modifications and temperature. J. Dairy Sci. 66, 2294-2302 (1983).
24. Buist, G. et al. Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of lactococcus lactis, a muramidase needed for cell separation. J. Bacteriol. 177, 1554-1563 (1995).
25. Wasserman, S.A., Daub, E., Grisafi, P., Botstein, D. & Walsh, C.T. Catabolic alanine racemase from Salmonella typhimurium: DNA sequence, enzyme purification and characterization. Biochemistry 23, 5182-5187 (1984).
26. Wang, E. & Walsh, C. Suicides subtrates for the alanine racemase of Escherichia coli B. Biochemistry 17, 1313-1321 (1978).
27. Lobocka, M., Henning, J., Wild, J. & Klopotowski, T. Organization and expression of the Escherichia coli K-12 dad operon encoding the smaller subunit of the D-amino acid dehydrogenase and the catabolic alanine racemase. J. Bacteriol. 176, 1500-1510 (1994).
28. Ferrari, E., Henner, D.J. & Yang, M.Y. Isolation of an alanine gene from Bacillus subtilis and its use for plasmid maintenance in B. subtilis. Bio/Technology 3, 1003-1007 (1985).
29. Hols, P. et al. The alanine racemase gene is essential for growth of Lactobacillus plantarum, J. Bacteriol. 179, 3804-3807 (1997).
30. Ohta, K., Beall, D.S., Mejia, J.P., Shanmugan, K.T. & Ingram, L.O. Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl. Environ. Microbiol. 57, 893-900 (1991).
31. Ohta, T., Ogboma, J.C., Tanaka, H. & Yajima, M. Development of a fermentation method using immobilized cells under unsterile conditions. 2. Ethanol and L-lactic acid production without heat and filter sterilization. Appl. Microbiol. Biotechnol. 42, 246-250 (1994).
32. Casadaban, M.J. & Cohen, S.N. Analysis of gene control signals by DNA fusion cloning in Escherichia coli. J. Mol. Biol. 138, 179-207 (1980).
33. Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular cloning: a laboratory manual 2nd edn (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY; 1989).
34. Vos, P. et al. A maturation protein essential for the production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope. J. Bacteriol. 171, 2795-2802 (1989).
35. Leenhouts, K.J., Kok, J. & Venema, G. Campbell-like integration of heterologous plasmid DNA into the chromosome of Lactococcus lactis subsp. lactis. Appl. Environ. Microbiol. 55, 394-400 (1989).
36. Wells, J.M., Wilson, P.W. & Le Page, R.W.F. Improved cloning vectors and transformation procedure for Lactococcus lactis. J. Appl. Bacteriol. 74, 629-636 (1993).
37. Ferain, T., Garmyn, D., Bernard, N., Hols, P. & Delcour, J. Lactobacillus plantarum idhL gene: overproduction and deletion. J. Bacteriol. 176, 596-601 (1994).
38. Chen, J.-D. & Morisson, D.A. Construction and properties of a new insertion vector, pJDC9, that is protected by transcriptional terminators and useful for the cloning of DNA from Streptococcus pneumoniae. Gene 64, 155-164 (1988).
39. Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 27, 680-685 (1970).
40. Starrenburg, M.J.C. & Hugenholtz, J. Citrate fermentation by Lactococcus and Leuconostoc spp. Appl. Environ. Microbiol. 57, 3535-3540 (1991).