thyA as a Selection Marker in Construction of Food-Grade Host-Vector and Integration Systems for Streptococcus thermophilus

Applied and Environmental Microbiology

Volumn 70, Issue 3, 2004, Pages 1858-1864

  Sasaki, Yasuko ^a   Ito, Yoshiyuki ^a   Sasaki, Takashi ^a  

^a MEIJI DAIRIES CORPORATION   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMARKERS; CELL CULTURE; CHROMOSOMES; CLONING; GENES;

DOUBLE CROSSOVER EVENTS; FOOD APPLICATIONS;

MICROBIOLOGY;

AMYLASE; ERYTHROMYCIN; GENE PRODUCT; PROTEIN AMYA; PROTEIN THYA; THYMIDYLATE SYNTHASE; TRIMETHOPRIM; UNCLASSIFIED DRUG;

BACTERIUM;

ANTIBIOTIC RESISTANCE; ARTICLE; AUTOTROPHY; BACTERIUM CULTURE; CHROMOSOME; CONTROLLED STUDY; FOOD; GENE CONSTRUCT; GENE EXPRESSION; GENETIC SELECTION; HOST; LACTOBACILLUS DELBRUECKII; MARKER GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; STREPTOCOCCUS THERMOPHILUS; TEMPERATURE DEPENDENCE;

BASE SEQUENCE; DNA, BACTERIAL; FOOD MICROBIOLOGY; FOOD TECHNOLOGY; GENES, BACTERIAL; GENETIC MARKERS; GENETIC VECTORS; HUMANS; LACTOBACILLUS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; RESTRICTION MAPPING; STREPTOCOCCUS; THYMIDYLATE SYNTHASE; TRANSFORMATION, GENETIC; YOGURT;

BACTERIA (MICROORGANISMS); LACTOBACILLUS; LACTOBACILLUS DELBRUECKII SUBSP. BULGARICUS; POSIBACTERIA; STREPTOCOCCUS THERMOPHILUS;

EID: 1642267393     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.3.1858-1864.2004     Document Type: Article

References (39)

1. Ajdic, D., W. M. McShan, R. E. McLaughlin, G. Savic, J. Chang, M. B. Carson, C. Primeaux, R. Tian, S. Kenton, H. Jia, S. Lin, Y. Qian, S. Li, H. Zhu, F. Najar, H. Lai, J. White, B. A. Roe, and J. J. Ferrett. 2002. Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen. Proc. Natl. Acad. Sci. USA 99:14434-14439.
2. Anastasiou, R., M. Papadelli, M. D. Georgalaki, G. Kalantzopoulos, and E. Tsakalidou. 2002. Cloning and sequencing of the gene encoding X-prolyl-dipeptidyl aminopeptidase (PepX) from Streptococcus thermophilus strain ACA-DC 4. J. Appl. Microbiol. 93:52-59.
3. Baccigalupi, L., G. Naclerio, M. D. Felice, and E. Ricca. 2000. Efficient insertional mutagenesis in Streptococcus thermophilus. Gene 258:9-14.
4. Biswas, I., A. Gruss, S. D. Ehrlich, and E. Maguin. 1993. High-efficiency gene inactivation and replacement system for gram-positive bacteria. J. Bacteriol. 175:3628-3635.
5. Bolotin, A., P. Wincker, S. Mauger, O. Jaillon, K. Malarme, J. Weissenbach, S. D. Ehrlich, and A. Sorokin. 2001. The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403. Genome Res. 11:731-753.
6. Cohen, S. N., A. C. Y. Chang, and L. Hsu. 1972. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. USA 69:2110.
7. Coll, P. F., V. R. Ausina, J. V. Vernis, B. O. Mirelis, and G. P. Prats. 1984. Exogenous thymidine and reversal of the inhibitory effect of sulfamethoxazole-trimethoprim on streptococci. Eur. J. Microbiol. 3:424-426.
8. de Ruyter, P. G. G. A., O. P. Kuipers, and W. M. de Vos. 1996. Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin. Appl. Environ. Microbiol. 62:3662-3666.
9. de Vos, W. M. 1999. Safe and sustainable systems for food-grade fermentations by genetically modified lactic acid bacteria. Int. Dairy J. 9:3-10.
10. Dickely, F., D. Nilsson, E. B. Hansen, and E. Johansen. 1995. Isolation of Lactococcus lactis nonsense suppressors and construction of a food-grade cloning vector. Mol. Microbiol. 15:839-847.
11. El Demerdash, H. A. M., K. J. Heller, and A. Geis. 2003. Application of the shsp gene, encoding a small heat shock protein, as a food-grade selection marker for lactic acid bacteria. Appl. Environ. Microbiol. 69:4408-4412.
12. Farmer, J. L., and F. Rothman. 1965. Transformable thymine-requiring mutant of Bacillus subtilis. J. Bacteriol. 89:262-263.
13. Fu, X., and J.-G. Xu. 2000. Development of a chromosome-plasmid balanced lethal system for Lactobacillus acidophilus with thyA gene as selective marker. Microbiol. Immunol. 44:551-556.
14. Hashiba, H., R. Takiguchi, K. Jyoho, and K. Aoyama. 1992. Establishment of a host-vector system in Lactobacillus helveticus with beta-galactosidase activity as a selection marker. Biosci. Biotechnol. Biochem. 56:190-194.
15. Herman, R. E., and L. L. McKay. 1986. Cloning and expression of the β-D-galactosidase gene from Streptococcus thermophilus in Escherichia coli. Appl. Environ. Microbiol. 52:45-50.
16. Holo, H., and I. F. Nes. 1989. High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl. Environ. Microbiol. 55:3119-3123.
17. Hols, P., T. Ferain, D. Garmyn, N. Bernard, and J. Delcour. 1994. Use of homologous expression-secretion signals and vector-free stable integration in engineering of Lactobacillus plantarum for α-amylase and levanase expression. Appl. Environ. Microbiol. 60:1401-1413.
18. Hoskins, J., W. E. Alborn, Jr., J. Arnold, L. C. Blaszczak, S. Burgett, B. S. DeHoff, S. T. Estrem, L. Fritz, D.-J. Fu, W. Fuller, C. Geringer, R. Gilmour, J. S. Glass, H. Khoja, A. R. Kraft, R. E. Lagace, D. J. LeBlanc, L. N. Lee, E. J. Lefkowitz, J. Lu, P. Matsushima, S. M. McAhren, M. McHenney, K. McLeaster, C. W. Mundy, T. I. Nicas, F. H. Norris, M. O'Gara, R. B. Peery, G. T. Robertson, P. Rockey, P.-M. Sun, M. E. Winkler, Y. Yang, M. Young-Bellido, G. Zhao, C. A. Zook, R. H. Baltz, S. R. Jaskunas, P. R. Rosteck, Jr., P. L. Skatrud, and J. I. Glass. 2001. Genome of the bacterium Streptococcus pneumoniae strain R6. J. Bacteriol. 183:5709-5717.
19. Huovinen, P. 1987. Trimethoprim resistance. Antimicrob. Agents Chemother. 31:1451-1456.
20. Ito, Y., Y. Sasaki, and T. Sasaki. June 1991. Novel plasmid pBUL1 derived from Lactobacillus and derivative thereof. Japanese patent P8103275.
21. Ito, Y., Y. Sasaki, and T. Sasaki. December 1991. Novel plasmid pSY1 derived from Lactococcus. Japanese patent P5176776.
22. Leenhouts, K., A. Bolhuis, G. Venema, and J. Kok. 1998. Construction of a food-grade multiple-copy integration system for Lactococcus lactis. Appl. Microbiol. Biotechnol. 49:417-423.
23. Maguin, E., H. Prévost, S. D. Ehrlich, and A. Gruss. 1996. Efficient insertional mutagenesis in lactococci and other gram-positive bacteria. J. Bacteriol. 178:931-935.
24. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
25. Mollet, B., J. Knol, B. Poolman, O. Marciset, and M. Delley. 1993. Directed genenomic integration, gene replacement, and integrative gene expression in Streptococcus thermophilus. J. Bacteriol. 175:4315-4324.
26. Nauta, A., D. van Sinderen, H. Karsens, E. Smit, G. Venema, and J. Kok. 1996. Inducible gene expression mediated by a repressor-operator system isolated from Lactococcus lactis bacteriophage rlt. Mol. Microbiol. 19:1331-1341.
27. Okada, T., K. Yanagisawa, and F. J. Ryan. 1960. Elective production of thymine-less mutants. Nature 188:340-341.
28. O'Sullivan, D., S. A. Walker, S. G. West, and T. R. Klaenhammer. 1996. Development of an expression strategy using a lytic phage to trigger explosive plasmid amplification and gene expression. Bio/Technology 14:82-87.
29. Pikis, A., J. A. Donkersloot, W. J. Rodriguez, and J. M. Keith. 1998. A conservative amino acid mutation in the chromosome-encoded dihydrofolate reductase confers trimethoprim resistance. J. Infect. Dis. 178:700-706.
30. Pinter, K., V. J. Davisson, and D. V. Santi. 1988. Cloning, sequencing, and expression of the Lactobacillus casei thymidylate synthase gene. DNA 7:235-241.
31. Posno, M., P. T. H. M. Heuvelmans, M. J. F. van Giezen, B. C. Lokman, R. J. Leer, and P. H. Pouwels. 1991. Complementation of the inability of Lactobacillus strains to utilize D-xylose with D-xylose catabolism-encoding genes of Lactobacillus pentosus. Appl. Environ. Microbiol. 57:2764-2766.
32. Ross, P., F. O'Gara, and S. Condon. 1990. Cloning and characterization of the thymidylate synthase gene from Lactococcus lactis subsp. lactis. Appl. Environ. Microbiol. 56:2156-2163.
33. Ross, P., F. O'Gara, and S. Condon. 1990. Thymidylate synthetase gene from Lactococcus lactis as a genetic marker: an alternative to antibiotic resistance genes. Appl. Environ. Microbiol. 56:2164-2169.
34. Sanders, J. W., G. Venema, and J. Kok. 1997. A chloride-inducible gene expression cassette and its use in induced lysis of Lactococcus lactis. Appl. Environ. Microbiol. 63:4877-4882.
35. Satoh, E., Y. Ito, Y. Sasaki, and T. Sasaki. 1997. Application of the extracellular α-amylase gene from Streptococcus bovis 148 to construction of a secretion vector for yogurt starter strains. Appl. Environ. Microbiol. 63: 4593-4596.
36. Schroeder, C. J., C. Robert, G. Lenzen, L. L. McKay, and A. Mercenier. 1991. Analysis of the lacZ sequences from two Streptococcus thermophilus strains: comparison with the Escherichia coli and Lactobacillus bulgaricus beta-galactosidase sequences. J. Gen. Microbiol. 137:369-380.
37. Steidler, L., S. Neirynck, N. Huyghebaert, V. Snoeck, A. Vermeire, B. Goddeeris, E. Cox, J. P. Remon, and E. Remaut. 2003. Biological containment of genetically modified Lactococcus lactis for intestinal delivery of human interleukin 10. Nat. Biotechnol. 21:785-789.
38. Tettelin, H., V. Masignani, M. J. Cieslewicz, J. A. Eisen, S. Peterson, M. R. Wessels, I. T. Paulsen, K. E. Nelson, I. Margarit, T. D. Read, L. C. Madoff, A. M. Wolf, M. J. Beanan, L. M. Brinkac, S. C. Daugherty, R. T. DeBoy, S. Durkin, J. F. Kolonay, L. A. Umayam, R. Madupu, M. R. Lewis, D. Radune, N. B Fedorova, D. Scanlan, H. Khouri, S. Mulligan, H. A. Carty, R. T. Cline, J. Gill, M. Scarselli, M. Mora, E. T. Iacobini, C. Brettoni, G. Galli, M. Mariani, F. Vegni, D. Maione, D. Rinaudo, R. Rappuoli, J. L. Telford, D. L. Kasper, G. Grandi, and C. M. Fraser. 2002. Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae. Proc. Natl. Acad. Sci. USA 99:12391-12396.
39. Wong, W. Y., P. Su, G. E. Allison, C.-Q. Liu, and N. W. Dunn. 2003. A potential food-grade cloning vector for Streptococcus thermophilus that uses cadmium resistance as the selectable marker. Appl. Environ. Microbiol. 69:5767-5771.