In vitro expression, monoclonal antibody and bioactivity for capsid protein of porcine circovirus type II without nuclear localization signal

Journal of Biotechnology

Volumn 118, Issue 2, 2005, Pages 201-211

  Zhou, Ji Yong ^a,b   Shang, Shao Bin ^a   Gong, Hui ^a   Chen, Qing Xin ^a,c   Wu, Jian Xiang ^a   Shen, Hui Gang ^a   Chen, Ting Fei ^a   Guo, Jun Qing ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

^c ZHEJIANG UNIVERSITY   (China)

Author keywords

Immunorelevant epitope; Monoclonal antibody; Porcine circovirus type II; Recombinant Capsid protein defecting nuclear localization signal

Indexed keywords

ANTIGENS; BINDING ENERGY; CELLS; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; GENES; REACTION KINETICS; SIGNAL PROCESSING; VACCINES;

IN VITRO EXPRESSION; NUCLEAR LOCALIZATION SIGNAL (NLS); PORCINE CIRCOVIRUS TYPE II (PCV2); SERODIAGNOSTIC REAGENT;

PROTEINS;

ANTISERUM; CAPSID PROTEIN; EPITOPE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MONOCLONAL ANTIBODY; NEUTRALIZING ANTIBODY; PROTEIN ANTIBODY; RECOMBINANT PROTEIN; VACCINE; VIRUS ANTIBODY;

ANIMAL CELL; ANTIGEN BINDING; ARTICLE; CIRCOVIRIDAE; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; NUCLEAR LOCALIZATION SIGNAL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; VIRUS GENE; VIRUS PARTICLE;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CAPSID PROTEINS; CELL LINE; CIRCOVIRUS; NUCLEAR LOCALIZATION SIGNALS; RECOMBINANT FUSION PROTEINS; SWINE; VIRAL VACCINES;

ESCHERICHIA COLI; MIRIDAE; PORCINE CIRCOVIRUS; SUS SCROFA;

EID: 21244479395     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.02.017     Document Type: Article

References (38)

1. G.M. Allan, and J.A. Ellis Porcine circoviruses: a review J. Vet. Diagn. Invest. 12 2000 3 14
2. G.M. Allan, S. Kennedy, F. McNeilly, J.C. Foster, J.A. Ellis, S.J. Krakowka, B.M. Meehan, and B.M. Adair Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus J. Comp. Pathol. 121 1999 1 11
3. G.M. Allan, F. McNeilly, J.P. Cassidy, G.A. Reilly, B. Adair, W.A. Ellis, and M.S. McNulty Pathogenesis of porcine circovirus: experimental infections of colostrum deprived piglets and examination of pig foetal material Vet. Microbiol. 44 1995 49 64
4. G.M. Allan, F. McNeilly, S. Kennedy, B. Daft, E.G. Clarke, J.A. Ellis, D.M. Haines, B.M. Meehan, and B.M. Adair Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the USA and Europe J. Vet. Diagn. Invest. 10 1998 3 10
5. M. Balasch, J. Segales, C. Rosell, M. Domingo, A. Mankertz, A. Urniza, and J. Plana-Duran Experimental inoculation of conventional pigs with tissue homogenates from pigs with post-weaning multisystemic wasting syndrome J. Comp. Pathol. 121 1999 139 148
6. P. Blanchard, D. Mahe, R. Cariolet, C. Truong, M. Le Dimna, C. Arnauld, N. Rose, E. Eveno, E. Albina, F. Madec, and A. Jestin An ORF2 protein-based ELISA for porcine circovirus type 2 antibodies in post-weaning multisystemic wasting syndrome Vet. Microbiol. 94 2003 183 194
7. C. Chae Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology Vet. J. 168 2004 41 49
8. G.F. Chen, and M. Inouye Suppression of the negative effect of minor arginine codonson gene expression, preferential usage of minor codons with in the first 25 codons of the Escherichia coli genes Nucleic Acids Res. 18 1990 1465 1473
9. A.K. Cheung, and S.R. Bolin Kinetics of porcine circovirus type 2 replication Arch. Virol. 147 2002 43 58
10. E.G. Clark Post-weaning wasting syndrome Proc. Am. Assoc. Swine Pract. 28 1997 499 501
11. J. Ellis, L. Hassard, E. Clark, J. Harding, G. Allan, P. Willson, J. Strokappe, K. Martin, F. McNeilly, B. Meehan, D. Todd, and D. Haines Isolation of circoviruses from lesions of pigs with postweaning multisystemic wasting syndrome Can. Vet. J. 39 1998 44 51
12. M. Fenaux, P.G. Halbur, M. Gill, T.E. Toth, and X.J. Meng Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2 J. Clin. Microbiol. 38 2000 2494 2503
13. A.L. Hamel, L.L. Lin, and G.P. Nayar Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs J. Virol. 72 1998 5262 5267
14. J. Harding Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation Proc. Am. Assoc. Swine Pract. 28 1997 503
15. C.J. Huang, J.J. Hung, C.Y. Wu, and M.S. Chien Multiplex PCR for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses Vet. Microbiol. 101 2004 209 214
16. S. Kennedy, D. Moffett, F. McNeilly, B. Meehan, J. Ellis, S. Krakowka, and G.M. Allan Reproduction of lesions of postweaning multisystemic wasting syndrome by infection of conventional pigs with porcine circovirus type 2 alone or in combination with porcine parvovirus J. Comp. Pathol. 122 2000 9 24
17. P. Lekcharoensuk, I. Morozov, P.S. Paul, N. Thangthumniyom, W. Wajjawalku, and X.J. Meng Epitope mapping of the major capsid protein of type 2 porcine circovirus (PCV2) by using chimeric PCV1 and PCV2 J. Virol. 78 2004 8135 8145
18. C. Liu, T. Ihara, T. Nunoya, and S. Ueda Development of an ELISA based on the baculovirus-expressed capsid protein of porcine circovirus type 2 as antigen J. Vet. Med. Sci. 66 2004 237 242
19. Q. Liu, S.K. Tikoo, and L.A. Babiuk Nuclear localization of the ORF2 protein encoded by porcine circovirus type 2 Virology 285 2001 91 99
20. Q. Liu, P. Willson, S. Attoh-Poku, and L.A. Babiuk Bacterial expression of an immunologically reactive PCV2 ORF2 fusion protein Protein Exp. Purif. 21 2001 115 120
21. D. Mahe, P. Blanchard, C. Truong, C. Arnauld, P. Le Cann, R. Cariolet, F. Madec, E. Albina, and A. Jestin Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes J. Gen. Virol. 81 2000 1815 1824
22. A. Mankertz, J. Mankertz, K. Wolf, and H.J. Buhk Identification of a protein essential for replication of porcine circovirus J. Gen. Virol. 79 1998 381 384
23. F. McNeilly, I. McNair, D.P. Mackie, B.M. Meehan, S. Kennedy, D. Moffett, J. Ellis, S. Krakowka, and G.M. Allan Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2 Arch. Virol. 146 2001 909 922
24. B.M. Meehan, J.L. Creelan, M.S. McNulty, and D. Todd Sequence of porcine circovirus DNA: affinities with plant circoviruses J. Gen. Virol. 78 1997 221 227
25. B.M. Meehan, F. McNeilly, D. Todd, S. Kennedy, A. Jewhurst, J.A. Ellis, L.E. Hassard, E.G. Clark, D.M. Haines, and G.M. Allan Characterization of novel circovirus DNAs associated with wasting syndromes in pigs J. Gen. Virol. 79 1998 2171 2179
26. I. Morozov, T. Sirinarumitr, S.D. Sorden, P.G. Halbur, M.K. Morgan, K.J. Yoon, and P.S. Paul Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome J. Clin. Microbiol. 36 1998 2535 2541
27. P. Nawagitgul, P.A. Harms, I. Morozov, B.J. Thacker, S.D. Sorden, C. Lekcharoensuk, and P.S. Paul Modified indirect porcine circovirus(PCV) type 2-based and recombinant capsid protein (ORF2)-based enzyme-linked immunosorbent assays for detection of antibodies to PCV Clin. Diagn. Lab. Immunol. 9 2002 33 40
28. P. Nawagitgul, I. Morozov, S.R. Bolin, P.A. Harms, S.D. Sorden, and P.S. Paul Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein J. Gen. Virol. 81 2000 2281 2287
29. F.D. Niagro, A.N. Forsthoefel, R.P. Lawther, L. Kamalanathan, B.W. Ritchie, K.S. Latimer, and P.D. Lukert Beak and feather disease virus and porcine circovirus genomes: intermediates between geminiviruses and plant circoviruses Arch. Virol. 143 1998 1723 1744
30. J. Sambrook, and D.W. Russell SDS-polyacrylamide gel electrophoresis of proteins, staining SDS-polyacrylamide gels and immunoblotting Molecular Cloning: A Laboratory Manual 3rd ed. 2001 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY pp. A8.40-A8.54
31. J. Segales, and M. Domingo Postweaning multisystemic wasting syndrome (PMWS) in pigs. A review Vet. Quart. 24 2002 109 124
32. I. Tischer, H. Gelderblom, W. Vettermann, and M.A. Koch A very small porcine virus with circular single-stranded DNA Nature 295 1982 64 66
33. I. Tischer, W. Mields, D. Wolff, M. Vagt, and W. Greim Studies on epidemiology and pathogenicity of porcine circovirus Arch. Virol. 91 1986 271 276
34. C. Truong, D. Mahe, P. Blanchard, M. Le Dimna, F. Madec, A. Jestin, and E. Albina Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection Arch. Virol. 146 2001 1197 1211
35. S.P. Zhang, G. Zubay, and E. Goldman Low-usage codons in Escherichia coli, yeast, fruit fly and primates Gene 105 1991 61 72
36. J.Y. Zhou, Q.X. Chen, J.X. Ye, H.G. Shen, T.F. Chen, and S.B. Shang Serological investigation and genomic characterization of PCV2 isolates from different geographic regions of Zhejiang province in China Vet. Res. Commun. 2005 29
37. J.Y. Zhou, J.X. Wu, L.Q. Cheng, X.J. Zheng, H. Gong, S.B. Shang, and E.M. Zhou Expression of immunogenic S1 glycoprotein of infectious bronchitis virus in transgenic potatoes J. Virol. 77 2003 9090 9093
38. J.Y. Zhou, L.Q. Cheng, X.J. Zheng, J.X. Wu, S.B. Shang, J.Y. Wang, and J.G. Chen Generation of the transgenic potato expressing full-length spike protein of infectious bronchitis virus J. Biotechnol. 111 2004 121 130