The Sec protein-translocation pathway

Trends in Microbiology

Volumn 9, Issue 10, 2001, Pages 494-500

  Mori, Hiroyuki ^a   Ito, Koreaki ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CARRIER PROTEIN; PROTEIN PRECURSOR; PROTEIN SEC; UNCLASSIFIED DRUG;

BACTERIUM; CELL MEMBRANE; CHANNEL GATING; CYTOSOL; DEGRADATION; ENZYME REGULATION; GENE CONTROL; MEMBRANE POTENTIAL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS; PROTEIN TRANSPORT; REGULATORY MECHANISM; REVIEW; RIBOSOME; SIGNAL TRANSDUCTION;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BIOLOGICAL TRANSPORT, ACTIVE; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; PROTEIN PRECURSORS; PROTEIN TRANSPORT;

EID: 0035477117     PISSN: 0966842X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0966-842X(01)02174-6     Document Type: Review

References (63)

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2. The Tat protein export pathway
3. Multiple pathways allow protein secretion across the bacterial outer membrane
4. Biogenesis of the Gram-negative bacterial envelope
5. YidC mediates membrane protein insertion in bacteria
6. Evolutionarily related insertion pathways of bacterial, mitochondrial, and thylakoid membrane proteins
7. Translocation, folding, and stability of the HflKC complex with signal anchor topogenic sequences
8. Sec-dependent membrane protein insertion: Sequential interaction of nascent FtsQ with SecY and YidC
9. Reconstitution of Sec-dependent membrane protein insertion: Nascent FtsQ interacts with YidC in a SecYEG-dependent manner
10. In vitro analysis of the stop-transfer process during translocation across the cytoplasmic membrane of Escherichia coli
11. Sec-dependent membrane protein biogenesis: SecYEG, preprotein hydrophobicity and translocation kinetics control the stop-transfer function
12. SecB, a molecular chaperone with two faces
13. The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins
14. A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery
15. The targeting pathway of Escherichia coli presecretory and integral membrane proteins is specified by the hydrophobicity of the targeting signal
16. Export of protein in bacteria
17. Biogenesis of inner membrane proteins in Escherichia coli
18. Secretion monitor, SecM, undergoes self-translation arrest in the cytosol
19. Physiological basis for conservation of the signal recognition particle targeting pathway in Escherichia coli
20. Evolutionarily conserved binding of ribosomes to the translocation channel via the large ribosomal RNA
21. A 30-residue-long 'export initiation domain' adjacent to the signal sequence is critical for protein translocation across the inner membrane of Escherichia coli
22. SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coli
23. A mutation in secY that causes enhanced SecA insertion and impaired late functions in protein translocation
24. The early interaction of the outermembrane protein PhoE with the periplasmic chaperone Skp occurs at the cytoplasmic membrane
25. Distinct membrane binding properties of N- and C-terminal domains of Escherichia coli SecA ATPase
26. Genetic dissection of SecA: Suppressor mutations against the secY205 translocase defect
27. Two independent mechanisms down-regulate the intrinsic SecA ATPase activity
28. Cross-talk between catalytic and regulatory elements in a DEAD motor domain is essential for SecA function
29. A molecular switch in SecA protein couples ATP hydrolysis to protein translocation
30. Nucleotide binding activity of SecA homodimer is conformationally regulated by temperature and altered by prlD and azi mutations
31. Conformational stabilization and crystallization of the SecA translocation ATPase from Bacillus subtilis
32. Escherichia coli SecA shape and dimensions
33. Topology of the integral membrane form of Escherichia coli SecA protein reveals multiple periplasmically exposed regions and modulation by ATP binding
34. Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state
35. SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane
36. The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phase
37. Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation
38. Targeting of signal sequenceless proteins for export in Escherichia coli with altered protein translocase
39. Signal sequence recognition in posttranslational protein transport across the yeast ER membrane
40. Syd, a SecY-interacting protein, excludes SecA from the SecYE complex with an altered SecY24 subunit
41. An essential amino acid residue in the protein translocation channel revealed by targeted random mutagenesis of SecY
42. PrlA4 prevents the rejection of signal sequence defective preproteins by stabilizing the SecA-SecY interaction during the initiation of translocation
43. Coupled structure change of SecA and SecG revealed by the synthetic lethality of the secAcsR11 and ΔsecG::kan double mutant
44. SecG function and phospholipid metabolism in Escherichia coli
45. Roles of SecG in ATP- and SecA-dependent protein translocation
46. Dislocation of membrane proteins in FtsH-mediated proteolysis
47. Genetic analysis of an essential cytoplasmic domain of Escherichia coli SecY based on resistance to Syd, a SecY-interacting protein
48. Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking
49. Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighboring SecE
50. Mapping the sites of interaction between SecY and SecE by cysteine scanning mutagenesis
51. The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits
52. Projection structure and oligomeric properties of a bacterial core protein translocase
53. The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex
54. SecYEG assembles into a tetramer to form the active protein translocation channel
55. Evaluating the oligomeric state of SecYEG in preprotein translocase
56. Two SecG molecules present in a single protein translocation machinery are functional even after crosslinking
57. YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase
58. SecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particle
59. Dissecting the translocase and integrase functions of the Escherichia coli SecYEG translocon
60. Revised translation start site for secM defines an atypical signal peptide that regulates Escherichia coli secA expression
61. Enhancing effect of Bacillus subtilis Ffh, a homologue of the SRP54 subunit of the mammalian signal recognition particle, on the binding of SecA to precursors of secretory proteins in vitro
62. A mutation in the Escherichia coli secY gene that produces distinct effects on inner membrane protein insertion and protein export
63. The engagement of Sec61p in the ER dislocation process