Dissecting the catalytic mechanism of trypanosoma brucei trypanothione synthetase by kinetic analysis and computational modeling

Journal of Biological Chemistry

Volumn 288, Issue 33, 2013, Pages 23751-23764

  Leroux, Alejandro E ^a   Haanstra, Jurgen R ^b,c   Bakker, Barbara M ^b,c   Krauth Siegel, R Luise ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

^c VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; MATHEMATICAL MODELS;

CATALYTIC MECHANISMS; COMPUTATIONAL MODEL; KINETIC ANALYSIS; KINETIC PROFILES; MODEL APPROACH; PRODUCT INHIBITION; TRYPANOSOMA BRUCEI; TRYPANOTHIONE SYNTHETASE;

KINETICS;

ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; AMIDASE; GLUTATHIONE; GLUTATHIONYLSPERMIDINE; SPERMIDINE; SYNTHETASE; TRYPANOTHIONE SYNTHETASE; UNCLASSIFIED DRUG; AMIDE SYNTHASE; BUFFER; TRYPANOTHIONE;

ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME BINDING; ENZYME MECHANISM; HYDROLYSIS; IN VIVO STUDY; MATHEMATICAL COMPUTING; MOLECULAR DYNAMICS; MOLECULAR MODEL; NONHUMAN; PARASITE IDENTIFICATION; PREDICTION; PRIORITY JOURNAL; STEADY STATE; TRYPANOSOMA BRUCEI; ANALOGS AND DERIVATIVES; ANTAGONISTS AND INHIBITORS; BIOCATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; CYTOSOL; ENZYME SPECIFICITY; ENZYMOLOGY; KINETICS; METABOLISM; PH; TEMPERATURE; TIME; TRYPANOSOMA BRUCEI BRUCEI;

TRYPANOSOMA BRUCEI;

ADENOSINE TRIPHOSPHATASES; AMIDE SYNTHASES; AMIDOHYDROLASES; BIOCATALYSIS; BUFFERS; COMPUTER SIMULATION; CYTOSOL; ENZYME ASSAYS; GLUTATHIONE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; SPERMIDINE; SUBSTRATE SPECIFICITY; TEMPERATURE; TIME FACTORS; TRYPANOSOMA BRUCEI BRUCEI;

EID: 84882298171     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.483289     Document Type: Article

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