Mechanistic analysis of allosteric and non-allosteric effects arising from nanobody binding to two epitopes of the dihydrofolate reductase of Escherichia coli

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1834, Issue 10, 2013, Pages 2147-2157

  Oyen, David ^a,b   Wechselberger, Rainer ^c   Srinivasan, Vasundara ^a,b   Steyaert, Jan ^a,b   Barlow, John N ^a,b  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^b DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^c UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Allostery; Antibody; Crystallography; Enzyme; NMR

Indexed keywords

DIHYDROFOLATE REDUCTASE; EPITOPE; NANOBODY;

ALLOSTERISM; ARTICLE; BINDING SITE; COMPETITIVE INHIBITION; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; EPITOPE MAPPING; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL PERMEATION CHROMATOGRAPHY; IMMUNIZATION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PHAGE DISPLAY; PRIORITY JOURNAL;

ALLOSTERY; ANTIBODY; CDR; COMPLEMENTARITY-DETERMINING REGION; CRYSTALLOGRAPHY; DHF; DHFR; DIHYDROFOLATE; DIHYDROFOLATE REDUCTASE; ELISA; ENZYME; ENZYME-LINKED IMMUNO SORBENT ASSAY; NADPH; NANOBODY; NB; NMR; NUCLEAR MAGNETIC RESONANCE; PBS; PDB; PHOSPHATE-BUFFERED SALINE; PROTEIN DATA BANK; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TETRAHYDROFOLATE; THF;

ALLOSTERIC REGULATION; ALLOSTERIC SITE; ANIMALS; BIOCATALYSIS; CAMELIDS, NEW WORLD; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; EPITOPE MAPPING; EPITOPES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; KINETICS; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PEPTIDE LIBRARY; PROTEIN BINDING; SINGLE-DOMAIN ANTIBODIES; TETRAHYDROFOLATE DEHYDROGENASE;

ESCHERICHIA COLI;

EID: 84881501905     PISSN: 15709639     EISSN: 18781454     Source Type: Journal    
DOI: 10.1016/j.bbapap.2013.07.010     Document Type: Article

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