NMR identification of epitopes of lyme disease antigen OspA to monoclonal antibodies

Journal of Molecular Biology

Volumn 281, Issue 1, 1998, Pages 61-67

  Huang, Xiaolin ^a   Yang, Xiaohua ^b   Luft, Benjamin J ^b   Koide, Shohei ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b STONY BROOK UNIVERSITY   (United States)

Author keywords

Antigen antibody interactions; Deuteration; Epitope mapping; Heteronuclear NMR; Outer surface protein A

Indexed keywords

BACTERIAL ANTIGEN; DEUTERIUM; EPITOPE; IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY; OUTER MEMBRANE PROTEIN;

ARTICLE; BORRELIA BURGDORFERI; CARBOXY TERMINAL SEQUENCE; LYME DISEASE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; VACCINE PRODUCTION;

BACTERIA (MICROORGANISMS); BORRELIA; BORRELIA BURGDORFERI; SPIROCHAETALES;

EID: 0032493678     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1998.1930     Document Type: Article

References (36)

1. Anglister J., Naider F. Nuclear magnetic resonance for studying peptide-antibody complexes by transferred nuclear Overhauser effect difference spectroscopy. Methods Enzymol. 203:1991;228-241
2. Barbour A.G., Fish D. The biological and social phenomenon of Lyme disease. Science. 260:1993;1610-1616
3. Chen Y., Reizer J., Saier M.H., Fairbrother W.J., Wright P.E. Mapping the binding interfaces of the proteins of the bacterial phaphotransferase system, HPr and IIAglc. Biochemistry. 32:1993;32-37
4. Clackson T., Wells J.A. A hot spot of binding energy in a hormone-receptor interface. Science. 267:1995;383-386
5. Cunningham B.C., Wells J.A. Comparison of a structural and a functional epitope. J. Mol. Biol. 234:1993;554-563
6. Davies D.R., Cohen G.H. Interactions of protein antigens with antibodies. Proc. Natl Acad. Sci. USA. 93:1996;7-12
7. Delaglio F., Grzesiek S., Vuister G.W., Zhu G., Pfeifer J., Bax A. NMRPipe a multidimensional pectral processing system based on UNIX pipes. J. Biomol. NMR. 6:1995;277-293
8. Dunn J.J., Lade B.N., Barbour A.G. Outer surface protein A (OspA) from the Lyme disease spirochete, Borrelia burgdorferi high level expression and purification of a soluble recombinant form of OspA. Protein Expr. Purif. 1:1990;159-168
9. + binding site on the MurB enzyme by NMR. Nature Struct. Biol. 3:1996;995-997
10. Fikrig E., Barthold S.W., Kantor F.S., Flavell R.A. Protection of mice against the Lyme disease agent by immunizing with recombinant OspA. Science. 250:1990;553-556
11. Fikrig E., Barthold S.W., Persing D.H., Sun X., Kantor F.S., Flavell R.A. Borrelia burgdorferi strain 25015 characterization ofouter surface protein A and vaccination against infection. J. Immunol. 148:1992;2256-2260
12. Grzesiek S., Bax A., Clore G.M., Gronenborn A.M., Hu J.S., Kaufman J., Palmer I., Stahl S.J., Wingfield P.T. The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase. Nature Struct. Biol. 3:1996;340-345
13. Jiang W., Gorevic P.D., Dattwyler R.J., Dunn J.J., Luft B.J. Purification of Borrelia burgdorferi outer surface protein A (OspA) and analysis of antibody binding domains. Clin. Diagn. Lab. Immunol. 1:1994;406-412
14. Johnson B.A., Blevins R.A. NMRView a computer program or the visualization and analysis of NMR data. J. Biomol. NMR. 4:1994;603-614
15. Johnson B.J.B., Sviat S.L., Happ C.M., Dunn J.J., Frantz J.C., Mayer L.W., Piesman J. Incomplete protection of hamsters vaccinated with unlipidated OspA from Borrelia burgdorferi infection is associated with low levels of antibody to an epitope defined by mAb LA-2. Vaccine. 13:1995;1086-1094
16. Kabsch W., Sander C. Dictionary of protein secondary structure pattern recognition of hydrogen-bonded and geometrical features. Biopolymers. 22:1983;2577-2637
17. Kay L.E., Gardner K.H. Solution NMR spectroscopy beyond 25 kDa. Curr. Opin. Struct. Biol. 7:1997;722-731
18. Kay L.E., Keifer P., Saarinen T. Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity. J. Am. Chem. Soc. 114:1992;10663-10665
19. Kraulis P. MOLSCRIPT a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallog. 24:1991;946-950
20. Li H., Lawson C.L. Crystallization and preliminary X-ray analysis of Borrelia burgdorferi outer surface protein A (OspA) complexed with a murine monoclonal antibody Fab fragment. J. Struct. Biol. 115:1995;335-337
21. Li H., Dunn J.J., Luft B.J., Lawson C.L. Crystal structure of Lyme disease antigen outer surface protein A complexed with an Fab. Proc. Natl Acad. Sci. USA. 94:1997;3584-3589
22. Markus M.A., Dayie K.T., Matsudaira P., Wagner G. Effect of deuteration on the amide proton relaxation rates in proteins. Heteronuclear NMR experiments on Villin 4T. J. Magn. Reson. ser. B. 105:1994;192-195
23. McGrath B.C., Dunn J.J., Gorgone G., Luft B.J., Guttman D., Dykhuizen D. Identification of an immunologically important hypervariable domain of major outer surface protein A of Borrelia burgdorferi. Infect. Immunol. 63:1995;1356-1361
24. Muchmore D.C., McIntosh L.P., Russell C.B., Anderson D.E., Dahlquist F.W. Expression and nitrogen-15 labeling of proteins for proton and nitrogen-15 nuclear magnetic resonance. Methods Enzymol. 177:1989;44-73
25. Nicholls A., Sharp K.A., Honig B. Protein folding and association insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins: Struct. Funct. Genet. 11:1991;281-296
26. Paterson Y., Englander S.W., Roder H. An antibody binding site on cytochrome c defined by hydrogen exchange and two-dimensional NMR. Science. 249:1990;755-759
27. Pham T.N., Koide S. NMR studies of Borrelia burgdorferi OspA, a 28 kDa protein containing a single-layer β-sheet. J. Biomol. NMR. 1998;. in the press
28. Pham T.-N., Koide A., Koide S. A stable single-layer β-sheet without a hydrophobic core. Nature Struct. Biol. 5:1998;115-119
29. Saito N.G., Paterson Y. Nuclear magnetic resonance spectroscopy for the study of B-cell epitopes. Methods. 9:1996;516-524
30. Schaible U.E., Kramer M.D., Eichmann K., Modolell M., Museteanu C., Simon M.M. Monoclonal antibodies specific for the outer surface protein A (OspA) of Borrelia burgdorferi prevent Lyme borreliosis in severe combined immunodeficiency (scid) mice. Proc. Natl Acad. Sci. USA. 87:1990;3768-3772
31. Sears J.E., Fikrig E., Nakagawa T.Y., Deponte K., Marcantonio N., Kantor F.S., Flavell R.A. Molecular mapping of Osp-A medicated immunity against Borrelia burgdorferi, the agent of Lyme disease. J. Immunol. 147:1991;1995-2000
32. Steere A.C. Lyme disease. New Engl. J. Med. 321:1989;586-596
33. Tashiro M., Montelione G.T. Structures of bacterial immunoglobulin-binding doamins and their complexes with immunoglobulins. Curr. Opin. Struct. Biol. 5:1995;471-481
34. Tsang P., Rance M., Wright P.E. Isotope-edited nuclear magnetic resonance studies of Fab-peptide complexes. Methods Enzymol. 203:1991;241-261
35. Webster D.M., Henry A.H., Rees A.R. Antibody-antigen interactions. Curr. Opin. Struct. Biol. 4:1994;123-129
36. Wilson I.A., Stanfield R.L. Antibody-antigen interactions new structures and new conformational changes. Curr. Opin. Struct. Biol. 4:1994;857-867