Autoprotease Npro: Analysis of self-cleaving fusion protein

Journal of Chromatography A

Volumn 1304, Issue , 2013, Pages 92-100

  Wellhoefer, Martin ^a   Sprinzl, Wolfgang ^a   Hahn, Rainer ^a,b   Jungbauer, Alois ^a,b  

^a AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Autoprotease fusion protein; Inclusion body; Refolding kinetics; Reversed phase chromatography

Indexed keywords

FUSION PROTEINS; IMMOBILIZED METALS; INCLUSION BODIES; LINEAR CORRELATION; LOWER LIMIT OF DETECTIONS; LOWER LIMIT OF QUANTIFICATIONS; REFOLDING KINETICS; REVERSED PHASE CHROMATOGRAPHY;

HIGH PRESSURE LIQUID CHROMATOGRAPHY; METAL IONS; PEPTIDES;

CHROMATOGRAPHY;

EDDIE PEP6HIS PROTEIN; EDDIE PROTEIN; HYBRID PROTEIN; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; CELL INCLUSION; GEL PERMEATION CHROMATOGRAPHY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; LIMIT OF DETECTION; LIMIT OF QUANTITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN REFOLDING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; REVERSED PHASE LIQUID CHROMATOGRAPHY; VALIDATION PROCESS;

AUTOPROTEASE FUSION PROTEIN; INCLUSION BODY; REFOLDING KINETICS; REVERSED PHASE CHROMATOGRAPHY;

CALIBRATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; CLONING, MOLECULAR; ESCHERICHIA COLI; HISTIDINE; INCLUSION BODIES; KINETICS; OLIGOPEPTIDES; PEPTIDE HYDROLASES; PEPTIDES; PROTEIN REFOLDING; RECOMBINANT FUSION PROTEINS;

EID: 84881026555     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2013.06.062     Document Type: Article

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