Split intein facilitated tag affinity purification for recombinant proteins with controllable tag removal by inducible auto-cleavage

Journal of Chromatography A

Volumn 1218, Issue 18, 2011, Pages 2553-2560

  Lu, Wei ^a   Sun, Ziyong ^a,b   Tang, Yanchun ^a,b   Chen, Junyong ^a   Tang, Fengyuan ^a   Zhang, Jing ^a,b   Liu, Jian Ning ^a,b  

^a EAST CHINA NORMAL UNIVERSITY   (China)

^b NANJING UNIVERSITY   (China)

Author keywords

Immobilization; Protein expression; Protein splicing; Ssp DnaB derived mini intein; Trans splicing

Indexed keywords

IMMOBILIZATION; PROTEIN EXPRESSION; PROTEIN SPLICING; SSP DNAB DERIVED MINI-INTEIN; TRANS-SPLICING;

PURIFICATION;

PROTEINS;

BRAIN NATRIURETIC PEPTIDE; DNAB HELICASE; ERYTHRINA TRYPSIN INHIBITOR; HYBRID PROTEIN; INTEIN; MALTOSE BINDING PROTEIN; RECOMBINANT PROTEIN; THIOREDOXIN; TRYPSIN INHIBITOR; UNCLASSIFIED DRUG;

AFFINITY LABELING; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN TARGETING; PURIFICATION; TRANS SPLICING;

AFFINITY LABELS; CHROMATOGRAPHY, AFFINITY; DNAB HELICASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; IMMOBILIZED PROTEINS; INTEINS; MASS SPECTROMETRY; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

EID: 79954422992     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.02.053     Document Type: Article

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