A small bispecific protein selected for orthogonal affinity purification

Biotechnology Journal

Volumn 5, Issue 6, 2010, Pages 605-617

  Alm, Tove ^a   Yderland, Louise ^a   Nilvebrant, Johan ^a   Halldin, Anneli ^a   Hober, Sophia ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

Author keywords

Albumin binding domain; Bispecific binder; Orthogonal purification; Phage display; Z domain

Indexed keywords

ALBUMIN BINDING DOMAIN; BINDING DOMAIN; BISPECIFIC BINDER; PHAGE DISPLAY; Z-DOMAIN;

AMINO ACIDS; BINDERS; BINDING ENERGY; LIGANDS; MOLECULES; ORGANIC ACIDS; PROTEINS;

PURIFICATION;

ALBUMIN BINDING DOMAIN; BINDING PROTEIN; HUMAN SERUM ALBUMIN; PROTEIN A; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; GENE FUSION; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE; PROTEIN VARIANT; RANDOMIZATION;

AMINO ACID SEQUENCE; BINDING SITES; CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PEPTIDES; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SERUM ALBUMIN;

EID: 77953157561     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000041     Document Type: Article

References (28)

1. Hedhammar, M., Graslund, T., Hober, S., Protein engineering strategies for selective protein purification. Chem. Eng. Technol. 2005, 28, 1315-1325. (Pubitemid 41694144)
2. Nilsson, J., Stahl, S., Lundeberg, J., Uhlen, M. et al., Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr. Purif. 1997, 11, 1-16. (Pubitemid 27434126)
3. Kraulis, P. J., Jonasson, P., Nygren, P. A., Uhlen, M. et al., The serum albumin-binding domain of streptococcal protein G is a three-helical bundle:A heteronuclear NMR study. FEBS Lett. 1996, 378, 190-194. (Pubitemid 26015572)
4. Olsson, A., Eliasson, M., Guss, B., Nilsson, B. et al., Structure and evolution of the repetitive gene encoding streptococcal protein G. Eur. J. Biochem. 1987, 168, 319-324.
5. Gulich, S., Linhult, M., Nygren, P., Uhlen, M. et al., Stability towards alkaline conditions can be engineered into a protein ligand. J. Biotechnol. 2000, 80, 169-178. (Pubitemid 30450150)
6. Linhult, M., Binz, H. K., Uhlen, M., Hober, S., Mutational analysis of the interaction between albumin-binding domain from streptococcal protein G and human serum albumin. Protein Sci. 2002, 11, 206-213. (Pubitemid 34075783)
7. Johansson, M. U., Frick, I. M., Nilsson, H., Kraulis, P. J. et al., Structure, specificity, and mode of interaction for bacterial albumin-binding modules. J. Biol. Chem. 2002, 277, 8114-8120. (Pubitemid 34975650)
8. Lejon, S., Frick, I. M., Bjorck, L., Wikstrom, M. et al., Crystal structure and biological implications of a bacterial albumin binding module in complex with human serum albumin. J. Biol. Chem. 2004, 279, 42924-42928. (Pubitemid 39372183)
9. Uhlen, M., Guss, B., Nilsson, B., Gatenbeck, S. et al., Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications. J. Biol. Chem. 1984, 259, 1695-1702. (Pubitemid 14146555)
10. Hober, S., Nord, K., Linhult, M., Protein A chromatography for antibody purification. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2007, 848, 40-47.
11. Ruther, U., pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts. Nucleic Acids Res. 1982, 10, 5765-5772.
12. Sambrook, J., Fritsch, E. F., Maniatis, T., Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York 1989.
13. Nord, K., Nilsson, J., Nilsson, B., Uhlen, M. et al., A combinatorial library of an alpha-helical bacterial receptor domain. Protein Eng. 1995, 8, 601-608.
14. Nilsson, B., Moks, T., Jansson, B., Abrahmsen, L. et al., A synthetic IgG-binding domain based on staphylococcal protein A. Protein Eng. 1987, 1, 107-113.
15. Alm, T., Steen, J., Ottosson, J., Hober, S., High-throughput protein purification under denaturating conditions by the use of cation exchange chromatography. Biotechnol. J. 2007, 2, 709-716. (Pubitemid 47062230)
16. Studier, F. W., Moffatt, B. A., Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 1986, 189, 113-130.
17. Hochuli, E., Bannwarth, W., Dobeli, H., Gentz, R. et al., Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Bio/Technology 1988, 6, 1321-1325. (Pubitemid 18266749)
18. Griffiths, A. D., Williams, S. C., Hartley, O., Tomlinson, I. M. et al., Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 1994, 13, 3245-3260.
19. Schreiber, G., Fersht, A., R. Rapid, electrostatically assisted association of proteins. Nat. Struct. Biol. 1996, 3, 427-431.
20. Albeck, S., Schreiber, G., Biophysical characterization of the interaction of the beta-lactamase TEM-1 with its protein inhibitor BLIP. Biochemistry 1999, 38, 11-21. (Pubitemid 29035666)
21. Bishop, B., Koay, D. C., Sartorelli, A. C., Regan, L., Reengineering granulocyte colony-stimulating factor for enhanced stability. J. Biol. Chem. 2001, 276, 33465-33470. (Pubitemid 37384556)
22. Hecht, M. H., Hehir, K. M., Nelson, H. C., Sturtevant, J. M. et al., Increasing and decreasing protein stability: Effects of revertant substitutions on the thermal denaturation of phage lambda repressor. J. Cell. Biochem. 1985, 29, 217-224.
23. Pace, C. N., Scholtz, J. M., A helix propensity scale based on experimental studies of peptides and proteins. Biophys. J. 1998, 75, 422-427.
24. O'Neil, K. T., DeGrado, W. F., A thermodynamic scale for the helix-forming tendencies of the commonly occurring amino acids. Science 1990, 250, 646-651.
25. Nord, K., Gunneriusson, E., Ringdahl, J., Stahl, S. et al., Binding proteins selected from combinatorial libraries of an alpha- helical bacterial receptor domain. Nat. Biotechnol. 1997, 15, 772-777. (Pubitemid 27329481)
26. Wahlberg, E., Lendel, C., Helgstrand, M., Allard, P. et al., An affibody in complex with a target protein: Structure and coupled folding. Proc. Natl. Acad. Sci. USA 2003, 100, 3185-3190. (Pubitemid 36358200)
27. Hedhammar, M., Jung, H. R., Hober, S., Enzymatic cleavage of fusion proteins using immobilised protease 3C. Protein Expr. Purif. 2006, 47, 422-426. (Pubitemid 43729030)
28. Hedhammar, M., Stenvall, M., Lonneborg, R., Nord, O. et al., A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein. J. Biotechnol. 2005, 119, 133-146. (Pubitemid 41219542)