-
1
-
-
36749044744
-
pro fusion technology to produce proteins with authentic N termini in E. coli
-
DOI 10.1038/nmeth1116, PII NMETH1116
-
Achmüller C, Kaar W, Ahrer K, Wechner P, Hahn R, Werther F, Schmidinger H, Cserjan-Puschmann M, Clementschitsch F, Striedner G, Bayer K, Jungbauer A, Auer B. 2007. Npro fusion technology to produce proteins with authentic N termini in E. coli. Nat Methods 4(12):1037-1043. (Pubitemid 350201776)
-
(2007)
Nature Methods
, vol.4
, Issue.12
, pp. 1037-1043
-
-
Achmuller, C.1
Kaar, W.2
Ahrer, K.3
Wechner, P.4
Hahn, R.5
Werther, F.6
Schmidinger, H.7
Cserjan-Puschmann, M.8
Clementschitsch, F.9
Striedner, G.10
Bayer, K.11
Jungbauer, A.12
Auer, B.13
-
2
-
-
24044492592
-
Effect of pH on thermal- And chemical-induced denaturation of GFP
-
DOI 10.1385/ABAB:126:2:149
-
Alkaabi KM, Yafea A, Ashraf SS. 2005. Effect of pH on thermal- and chemical-induced denaturation of GFP. Appl Biochem Biotechnol 126(2):149-156. (Pubitemid 41213294)
-
(2005)
Applied Biochemistry and Biotechnology
, vol.126
, Issue.2
, pp. 149-156
-
-
Alkaabi, K.M.1
Yafea, A.2
Ashraf, S.S.3
-
3
-
-
1842454986
-
Fast and faster: A designed variant of the B-domain of protein a folds in 3 msec
-
DOI 10.1110/ps.03541304
-
Arora P, Oas TG, Myers JK. 2004. Fast and faster: A designed variant of the B-domain of protein A folds in 3 msec. Protein Sci 13(4):847-853. (Pubitemid 38429208)
-
(2004)
Protein Science
, vol.13
, Issue.4
, pp. 847-853
-
-
Arora, P.1
Oas, T.G.2
Myers, J.K.3
-
4
-
-
0029871341
-
Role of the N terminus in RNase a homologues: Differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity
-
DOI 10.1006/jmbi.1996.0218
-
Boix E, Wu Y, Vasandani VM, Saxena SK, Ardelt W, Ladner J, Youle RJ. 1996. Role of the N terminus in RNase A homologues: Differences in catalytic activity, ribonuclease inhibitor interaction and cytotoxicity. J Mol Biol 257(5):992-1007. (Pubitemid 26117085)
-
(1996)
Journal of Molecular Biology
, vol.257
, Issue.5
, pp. 992-1007
-
-
Boix, E.1
Wu, Y.N.2
Vasandani, V.M.3
Saxena, S.K.4
Ardelt, W.5
Ladner, J.6
Youle, R.J.7
-
5
-
-
0032534048
-
Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step
-
Chong S, Montello G, Zhang A, Cantor E, Liao W, Xu M, Benner J. 1998. Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step. Nucleic Acids Res 26(22):5109-5115. (Pubitemid 28517566)
-
(1998)
Nucleic Acids Research
, vol.26
, Issue.22
, pp. 5109-5115
-
-
Chong, S.1
Montello, G.E.2
Zhang, A.3
Cantor, E.J.4
Liao, W.5
Xu, M.-Q.6
Benner, J.7
-
6
-
-
26444526644
-
Sensor combination and chemometric modelling for improved process monitoring in recombinant E. coli fed-batch cultivations
-
DOI 10.1016/j.jbiotec.2005.05.030, PII S0168165605002932
-
Clementschitsch F, Kern J, Pötschacher F, Bayer K. 2005. Sensor combination and chemometric modelling for improved process monitoring in recombinant E. coli fed-batch cultivations. J Biotechnol 120(2):183-196. (Pubitemid 41429280)
-
(2005)
Journal of Biotechnology
, vol.120
, Issue.2
, pp. 183-196
-
-
Clementschitsch, F.1
Jurgen, K.2
Florentina, P.3
Karl, B.4
-
7
-
-
0030023486
-
The roles of partly folded intermediates in protein folding
-
Creighton TE, Darby NJ, Kemmink J. 1996. The roles of partly folded intermediates in protein folding. FASEB J 10:110-118. (Pubitemid 26035513)
-
(1996)
FASEB Journal
, vol.10
, Issue.1
, pp. 110-118
-
-
Creighton, T.E.1
Darby, N.J.2
Kemmink, J.3
-
8
-
-
1642290710
-
Microsecond folding dynamics of the F13W G29A mutant of the B domain of staphylococcal protein a by laser-induced temperature jump
-
DOI 10.1073/pnas.0306433101
-
Dimitriadis G, Drysdale A, Myers JK, Arora P, Radford SE, Oas TG, Smith DA. 2004. Microsecond folding dynamics of the F13W G29A mutant of the B domain of staphylococcal protein A by laser-induced temperature jump. Proc Natl Acad Sci USA 101(11):3809-3814. (Pubitemid 38381060)
-
(2004)
Proceedings of the National Academy of Sciences of the United States of America
, vol.101
, Issue.11
, pp. 3809-3814
-
-
Dimitriadis, G.1
Drysdale, A.2
Myers, J.K.3
Arora, P.4
Radford, S.E.5
Oas, T.G.6
Smith, D.A.7
-
9
-
-
0035969980
-
The additional methionine residue at the N-terminus of bacterially expressed human interleukin-2 affects the interaction between the N- And C-termini
-
DOI 10.1021/bi001170r
-
Endo S, Yamamoto Y, Sugawara T, Nishimura O, Fujino M. 2001. The additional methionine residue at the N-terminus of bacterially expressed human interleukin-2 affects the interaction between the N- and C-termini. Biochemistry 40(4):914-919. (Pubitemid 32108583)
-
(2001)
Biochemistry
, vol.40
, Issue.4
, pp. 914-919
-
-
Endo, S.1
Yamamoto, Y.2
Sugawara, T.3
Nishimura, O.4
Fujino, M.5
-
11
-
-
0032832463
-
Isolating inclusion bodies from bacteria
-
Georgiou G, Valax P. 1999. Isolating inclusion bodies from bacteria. Methods Enzymol 309:48-58.
-
(1999)
Methods Enzymol
, vol.309
, pp. 48-58
-
-
Georgiou, G.1
Valax, P.2
-
12
-
-
0032007853
-
Strategies for optimizing heterologous protein expression in Escherichia coli
-
DOI 10.1016/S0167-7799(97)01155-4, PII S0167779997011554
-
Hannig G, Makrides SC. 1998. Strategies for optimizing heterologous protein expression in Escherichia coli. Trends Biotechnol 16(2):54-60. (Pubitemid 28055472)
-
(1998)
Trends in Biotechnology
, vol.16
, Issue.2
, pp. 54-60
-
-
Hannig, G.1
Makrides, S.C.2
-
13
-
-
1842410676
-
Oxidative renaturation of lysozyme at high concentrations
-
DOI 10.1002/(SICI)1097-0290(19970505)54:3<221::AID-BIT3>3.0.CO;2-H
-
Hevehan DL, De Bernardez Clark E. 1997. Oxidative renaturation of lysozyme at high concentrations. Biotechnol Bioeng 54(3):221-230. (Pubitemid 27176594)
-
(1997)
Biotechnology and Bioengineering
, vol.54
, Issue.3
, pp. 221-230
-
-
Hevehan, D.L.1
De Bernardez Clark, E.2
-
14
-
-
0344815737
-
Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid
-
Hirel P-H, Schmitter J-M, Dessen P, Fayat G, Blanquet S. 1989. Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid. Proc Natl Acad Sci USA 86(21):8247-8251. (Pubitemid 19286036)
-
(1989)
Proceedings of the National Academy of Sciences of the United States of America
, vol.86
, Issue.21
, pp. 8247-8251
-
-
Hirel, P.-H.1
Schmitter, J.-M.2
Dessen, P.3
Fayat, G.4
Blanquet, S.5
-
15
-
-
33846371209
-
Current status of technical protein refolding
-
DOI 10.1016/j.jbiotec.2006.12.004, PII S0168165606010273
-
Jungbauer A, Kaar W. 2007. Current status of technical protein refolding. J Biotechnol 128(3):587-596. (Pubitemid 46135798)
-
(2007)
Journal of Biotechnology
, vol.128
, Issue.3
, pp. 587-596
-
-
Jungbauer, A.1
Kaar, W.2
-
16
-
-
14744271625
-
Protein aggregation in vitro and in vivo: A quantitative model of the kinetic competition between folding and aggregation
-
Kiefhaber T, Rudolph R, Kohler HH, Buchner J. 1991. Protein aggregation in vitro and in vivo: A quantitative model of the kinetic competition between folding and aggregation. Biotechnology 9(9):825-829.
-
(1991)
Biotechnology
, vol.9
, Issue.9
, pp. 825-829
-
-
Kiefhaber, T.1
Rudolph, R.2
Kohler, H.H.3
Buchner, J.4
-
17
-
-
0036772580
-
Preparative protein refolding
-
Middelberg APJ. 2002. Preparative protein refolding. Trends Biotechnol 20(10):437-443.
-
(2002)
Trends Biotechnol
, vol.20
, Issue.10
, pp. 437-443
-
-
Middelberg, A.P.J.1
-
18
-
-
2442718804
-
Protein splicing of a pyrococcus abyssi intein with a C-terminal glutamine
-
DOI 10.1074/jbc.M400887200
-
Mills KV, Manning JS, Garcia AM, Wuerdeman LA. 2004. Protein splicing of a Pyrococcus abyssi intein with a C-terminal glutamine. J Biol Chem 279(20):20685-20691. (Pubitemid 38656464)
-
(2004)
Journal of Biological Chemistry
, vol.279
, Issue.20
, pp. 20685-20691
-
-
Mills, K.V.1
Manning, J.S.2
Garcia, A.M.3
Wuerdeman, L.A.4
-
19
-
-
0031259833
-
Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins
-
DOI 10.1006/prep.1997.0767
-
Nilsson J, Stahl S, Lundeberg J, Uhlen M, Nygren PA. 1997. Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins. Protein Expr Purif 11(1):1-16. (Pubitemid 27434126)
-
(1997)
Protein Expression and Purification
, vol.11
, Issue.1
, pp. 1-16
-
-
Nilsson, J.1
Stahl, S.2
Lundeberg, J.3
Uhlen, M.4
Nygren, P.-A.5
-
20
-
-
0036203366
-
Mutational analysis of a blood coagulation factor VIII-binding peptide
-
DOI 10.1034/j.1399-3011.2002.1o965.x
-
Pflegerl K, Hahn R, Berger E, Jungbauer A. 2002. Mutational analysis of a blood coagulation factor VIII-binding peptide. J Pept Res 59(4):174-182. (Pubitemid 34275837)
-
(2002)
Journal of Peptide Research
, vol.59
, Issue.4
, pp. 174-182
-
-
Pflegerl, K.1
Hahn, R.2
Berger, E.3
Jungbauer, A.4
-
21
-
-
0031006611
-
Chromophore formation in green fluorescent protein
-
DOI 10.1021/bi970281w
-
Reid BG, Flynn GC. 1997. Chromophore formation in green fluorescent protein. Biochemistry 36(22):6786-6791. (Pubitemid 27242339)
-
(1997)
Biochemistry
, vol.36
, Issue.22
, pp. 6786-6791
-
-
Reid, B.G.1
Flynn, G.C.2
-
22
-
-
0030046574
-
In vitro folding of inclusion body proteins
-
Rudolph R, Lilie H. 1996. In vitro folding of inclusion body proteins. FASEB J 10(1):49-56.
-
(1996)
FASEB J 10
, vol.1
, pp. 49-56
-
-
Rudolph, R.1
Lilie, H.2
-
23
-
-
0031935816
-
N-terminal protease of pestiviruses: Identification of putative catalytic residues by site-directed mutagenesis
-
Rümenapf T, Stark R, Heimann M, Thiel HJ. 1998. N-terminal protease of pestiviruses: Identification of putative catalytic residues by site-directed mutagenesis. J Virol 72(3):2544-2547. (Pubitemid 28100822)
-
(1998)
Journal of Virology
, vol.72
, Issue.3
, pp. 2544-2547
-
-
Rumenapf, T.1
Stark, R.2
Heimann, M.3
Thiel, H.-J.4
-
24
-
-
0043135136
-
Continuous matrix-assisted refolding of proteins
-
Schlegl R, Iberer G, Machold C, Necina R, Jungbauer A. 2003. Continuous matrix-assisted refolding of proteins. J Chromatogr A 1009(1-2):119-132.
-
(2003)
J Chromatogr A
, vol.1009
, Issue.1-2
, pp. 119-132
-
-
Schlegl, R.1
Iberer, G.2
Machold, C.3
Necina, R.4
Jungbauer, A.5
-
25
-
-
0027424003
-
Processing of pestivirus polyprotein: Cleavage site between autoprotease and nucleocapsid protein of classical swine fever virus
-
Stark R, Meyers G, Rümenapf T, Thiel H-J. 1993. Processing of pestivirus polyprotein: Cleavage site between autoprotease and nucleocapsid protein of classical swine fever virus. J Virol 67(12):7088-7095. (Pubitemid 23343834)
-
(1993)
Journal of Virology
, vol.67
, Issue.12
, pp. 7088-7095
-
-
Stark, R.1
Meyers, G.2
Rumenapf, T.3
Thiel, H.-J.4
-
26
-
-
0034665455
-
Design of high-throughput methods of protein production for structural biology
-
Stevens RC. 2000. Design of high-throughput methods of protein production for structural biology. Structure 8(9):R177-R185.
-
(2000)
Structure
, vol.8
, Issue.9
-
-
Stevens, R.C.1
-
27
-
-
0036518147
-
Identification of in vitro folding conditions for procathepsin S and cathepsin S using fractional factorial screens
-
DOI 10.1006/prep.2001.1573
-
Tobbell DA, Middleton BJ, Raines S, Needham MRC, Taylor IWF, Beveridge JY, Abbott WM. 2002. Identification of in vitro folding conditions for Procathepsin S and Cathepsin S using fractional factorial screens. Protein Expr Purif 24(2):242-254. (Pubitemid 40047441)
-
(2002)
Protein Expression and Purification
, vol.24
, Issue.2
, pp. 242-254
-
-
Tobbell, D.A.1
Middleton, B.J.2
Raines, S.3
Needham, M.R.C.4
Taylor, I.W.F.5
Beveridge, J.Y.6
Abbott, W.M.7
-
28
-
-
0033015460
-
Rapid protein-folding assay using green fluorescent protein
-
DOI 10.1038/10904
-
Waldo GS, Standish BM, Berendzen J, Terwilliger TC. 1999. Rapid protein-folding assay using green fluorescent protein. Nat Biotechnol 17(7):691-695. (Pubitemid 29316148)
-
(1999)
Nature Biotechnology
, vol.17
, Issue.7
, pp. 691-695
-
-
Waldo, G.S.1
Standish, B.M.2
Berendzen, J.3
Terwilliger, T.C.4
-
29
-
-
0020482348
-
Reversible denaturation of Aequorea green-fluorescent protein: Physical separation and characterization of the renatured protein
-
Ward WW, Bokman SH. 1982. Reversible denaturation of Aequorea green-fluorescent protein: Physical separation and characterization of the renatured protein. Biochemistry 21(19):4535-4540.
-
(1982)
Biochemistry
, vol.21
, Issue.19
, pp. 4535-4540
-
-
Ward, W.W.1
Bokman, S.H.2
-
30
-
-
0018788361
-
Reconstitution of lactic dehydrogenase. Noncovalent aggregation vs. reactivation. 1. Physical properties and kinetics of aggregation
-
Zettlmeissl G, Rudolph R, Jaenicke R. 1979. Reconstitution of lactic dehydrogenase. Noncovalent aggregation vs. reactivation. 1. Physical properties and kinetics of aggregation. Biochemistry 18(25):5567-5571.
-
(1979)
Biochemistry
, vol.18
, Issue.25
, pp. 5567-5571
-
-
Zettlmeissl, G.1
Rudolph, R.2
Jaenicke, R.3
|