Matrix-assisted refolding of autoprotease fusion proteins on an ion exchange column: A kinetic investigation

Journal of Chromatography A

Volumn 1217, Issue 38, 2010, Pages 5950-5956

  Schmoeger, Elisabeth ^a,b   Wellhoefer, Martin ^a,b   Dürauer, Astrid ^a,b   Jungbauer, Alois ^a,b   Hahn, Rainer ^a,b  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

Author keywords

Autoprotease; Cleavage; Ion exchange chromatography; Productivity; Refolding

Indexed keywords

AUTOPROTEASE; BATCH REFOLDING PROCESS; CATION EXCHANGERS; CLEAVAGE; CONTACT TIME; FUSION PROTEINS; HIGH CONCENTRATION; ION EXCHANGE CHROMATOGRAPHY; ION-EXCHANGE COLUMNS; KINETIC INVESTIGATIONS; MATRIX; PROTEIN CONCENTRATIONS; PROTEIN LOADINGS; RECOMBINANT PROTEIN; REFOLDING; SELF-CLEAVAGE; SHORT CONTACT TIME;

CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; GELS; ION CHROMATOGRAPHY; ION EXCHANGERS; IONS; PRODUCTIVITY; PROTEINS;

ION EXCHANGE;

AUTOPROTEASE; ENZYME; HYBRID PROTEIN; MUTANT PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ELUTION; ENZYME ENGINEERING; ION EXCHANGE CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN CLEAVAGE; PROTEIN DENATURATION; PROTEIN FOLDING; TIME;

CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ESCHERICHIA COLI; KINETICS; PROTEIN REFOLDING; RECOMBINANT FUSION PROTEINS; VIRAL PROTEINS;

EID: 77956172307     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.07.053     Document Type: Article

References (28)

1. Burgess R.R. Methods Enzymol 2009, vol. 463:259. Academic Press, New York.
2. Creighton T.E., Darby N.J., Kemmink J. FASEB J. 1996, 10:110.
3. De Bernardez Clark E. Curr. Opin. Biotechnol. 1998, 9:157.
4. Jungbauer A., Kaar W. J. Biotechnol. 2007, 128:587.
5. Middelberg A.P.J. Trends Biotechnol. 2002, 20:437.
6. Varnerin J.P., Smith T., Rosenblum C.I., Vongs A., Murphy B.A., Nunes C., Mellin T.N., King J.J., Burgess B.W., Junker B., Chou M., Hey P., Frazier E., MacIntyre D.E., Van der Ploeg L.H.T., Tota M.R. Protein Expr. Purif. 1998, 14:335.
7. Yoshii H., Furuta T., Yonehara T., Ito D., Linko Y.Y., Linko P. Biosci. Biotechnol. Biochem. 2000, 64:1159.
8. West S.M., Chaudhuri J.B., Howell J.A. Biotechnol. Bioeng. 1998, 57:590.
9. Garza-Ramos G., Tuena De Gomez-Puyou A., Gracy R.W. Eur. J. Biochem. 1992, 208:389.
10. Forciniti D. J. Chromatogr. A 1994, 668:95.
11. Geng X., Wang C. J. Chromatogr. B 2007, 849:69.
12. Jungbauer A., Kaar W., Schlegl R. Curr. Opin. Biotechnol. 2004, 15:487.
13. Li M., Su Z.-G., Janson J.-C. Protein Expr. Purif. 2004, 33:1.
14. Park B.J., Lee C.H., Mun S., Koo Y.M. Process Biochem. 2006, 41:1072.
15. Haught C., Davis G.D., Subramanian R., Jackson K.W., Harrison R.G. Biotechnol. Bioeng. 1998, 57:55.
16. Hochuli E., Bannwarth W., Dobeli H., Gentz R., Stuber D. Biotechnology 1988, 6:1321.
17. Skosyrev V.S., Kulesskiy E.A., Yakhnin A.V., Temirov Y.V., Vinokurov L.M. Protein Expr. Purif. 2003, 28:350.
18. Smith D.B., Johnson K.S. Gene 1988, 67:31.
19. Achmüller C., Kaar W., Ahrer K., Wechner P., Hahn R., Werther F., Schmidinger H., Cserjan-Puschmann M., Clementschitsch F., Striedner G., Bayer K., Jungbauer A., Auer B. Nat. Methods 2007, 4:1037.
20. Rumenapf T., Stark R., Heimann M., Thiel H.J. J. Virol. 1998, 72:2544.
21. Kaar W., Ahrer K., Dürauer A., Greinstetter S., Sprinzl W., Wechner P., Clementschitsch F., Bayer K., Achmüller C., Auer B., Hahn R., Jungbauer A. Biotechnol. Bioeng. 2009, 104:774.
22. Schmoeger E., Berger E., Trefilov A., Jungbauer A., Hahn R. J. Chromatogr. A 2009, 1216:8460.
23. Machold C., Schlegl R., Buchinger W., Jungbauer A. J. Biotechnol. 2005, 117:83.
24. Chen Y., Leong S.S.J. J. Chromatogr. A 2009, 1216:4877.
25. Grillari J., Ajuh P., Stadler G., Löscher M., Voglauer R., Ernst W., Chusainow J., Eisenhaber F., Pokar M., Fortschegger K., Grey M., Lamond A.I., Katinger H. Nucleic Acids Res. 2005, 33:6868.
26. Clementschitsch F., Kern J., Pötschacher F., Bayer K. J. Biotechnol. 2005, 120:183.
27. Ueberbacher R., Dürauer A., Ahrer K., Mayer S., Sprinzl W., Jungbauer A., Hahn R. Process Biochem. 2009, 44:1217.
28. Kiefhaber T., Rudolph R., Kohler H.H., Buchner J. Biotechnology 1991, 9:825.