Enzymatic neutralization of the chemical warfare agent VX: Evolution of phosphotriesterase for phosphorothiolate hydrolysis

Journal of the American Chemical Society

Volumn 135, Issue 28, 2013, Pages 10426-10432

  Bigley, Andrew N ^a   Xu, Chengfu ^a   Henderson, Terry J ^b   Harvey, Steven P ^b   Raushel, Frank M ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

^b EDGEWOOD CHEMICAL BIOLOGICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL WARFARE AGENTS; DIRECTED EVOLUTION; ENVIRONMENTAL PERSISTENCES; ENZYMATIC EFFICIENCY; ORDERS OF MAGNITUDE; PHOSPHOTRIESTERASES; TOXIC SUBSTANCES; TURNOVER NUMBER;

CATALYST ACTIVITY; CHEMICAL WARFARE; LIBRARIES;

HYDROLYSIS;

CHEMICAL WARFARE AGENT; CHEMICAL WARFARE AGENT VX; ENZYME VARIANT; MUTANT PROTEIN; PHOSPHATE; PHOSPHONIC ACID DERIVATIVE; PHOSPHOROTHIOIC ACID DERIVATIVE; PHOSPHOTRIESTERASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYST; CHIRALITY; CONTROLLED STUDY; ENANTIOMER; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME KINETICS; ENZYME STRUCTURE; HYDROLYSIS; MOLECULAR LIBRARY; MUTAGENESIS; POLYMERASE CHAIN REACTION; RACEMIC MIXTURE; SCREENING; STEREOCHEMISTRY; WILD TYPE;

BIOCATALYSIS; HYDROLYSIS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; ORGANOTHIOPHOSPHORUS COMPOUNDS; PHOSPHATES; PHOSPHORIC TRIESTER HYDROLASES;

EID: 84880392305     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja402832z     Document Type: Article

References (30)

1. Maxwell, D. M.; Brecht, K. M.; Koplovitz, I.; Sweeney, R. E. Mol. Toxicol. 2006, 80, 756
2. Leikin, J. B.; Thomas, R. G.; Walter, F. G.; Klein, R.; Meislin, H. W. Crit. Care Med. 2002, 30, 2346
3. Columbus, I.; Waysbort, D.; Marcovitch, I.; Yehezkel, L.; Mizrahi, D. M. Environ. Sci. Technol. 2012, 46, 3921
4. Yang, Y. C. Chem. Ind. 1995, 9, 334
5. Yang, Y. C. Acc. Chem. Res. 1999, 32, 109
6. Saxina, A.; Sun, W.; Fedorko, J. M.; Koplovitz, I.; Doctor, B. P. Biochem. Pharmacol. 2011, 81, 164
7. Ashani, Y.; Pistinner, S. Toxicol. Sci. 2004, 77, 358
8. Saxena, A.; Tipparaju, P.; Luo, C.; Doctor, B. P. Process Biochem. 2010, 45, 1313
9. Cheng, T.-C.; Liu, L.; Wang, B.; Wu, J.; DeFrank, J. J.; Anderson, D. M.; Rastogi, V. K.; Hamilton, A. B. J. Ind. Microbiol. Biotechnol. 1997, 18, 49
10. Melzer, M.; Chen, J. C.-H.; Heidenreich, A.; Gab, J.; Koller, M.; Kehe, K.; Blum, M.-M. J. Am. Chem. Soc. 2009, 131, 17226
11. Kirby, S. D.; Norris, J. R.; Smith, J. R.; Bahnson, B. J.; Cerasoli, D. M. Chem. Biol. Interact. 2012, 203, 181
12. Gupta, R. D.; Goldsmith, M.; Ashani, Y.; Simo, Y.; Mullokandov, G.; Bar, H.; Ben-David, M.; Leader, H.; Margalit, R.; Silman, I.; Sussman, J. L.; Tawfik, D. S. Nat. Chem. Biol. 2011, 7, 120
13. Tsai, P.-C.; Fox, N.; Bigley, A. N.; Harvey, S. P.; Barondeau, D. P.; Raushel, F. M. Biochemistry 2012, 51, 6463
14. Lai, K.; Grimsley, J. K.; Kuhlmann, B. D.; Scapozza, L.; Harvey, S. P.; DeFrank, J. J.; Kolakowski, J. E.; Wild, J. R. Chimia 1996, 50, 430
15. Caldwell, S. R.; Newcomb, J. R.; Schlecht, K. A.; Raushel, F. M. Biochemistry 1991, 30, 7438
16. Benschop, H. P.; De Jong, L. P. A. Acc. Chem. Res. 1988, 21, 368
17. Ordentlich, A.; Barak, D.; Sod-Moriah, G.; Kaplan, D.; Mizrahi, D.; Segall, Y.; Kronman, C.; Karton, Y.; Lazar, A.; Marcus, D.; Velan, B.; Shafferman, A. Biochemistry 2004, 43, 11255
18. Tsai, P. C.; Bigley, A, N.; Li, Y.; Ghanem, E.; Cadieux, C. L.; Kasten, S. A.; Reeves, T. E.; Cerasoli, D. M.; Raushel, F. M. Biochemistry 2010, 49, 7978
19. Rastogi, V. K.; DeFrank, J. J.; Cheng, T.; Wild, J. R. Biochem. Biophys. Res. Commun. 1997, 241, 294
20. Hong, S.-B.; Raushel, F. M. Biochemistry 1996, 35, 10904
21. Vanhooke, J. L.; Benning, M. M.; Raushel, F. M.; Holden, H. M. Biochemistry 1996, 35, 6020
22. Chen-Goodspeed, M.; Sogorb, M. A.; Wu, F.; Hong, S.-B.; Raushel, F. M. Biochemistry 2001, 40, 1325
23. Afriat-Jurnou, L.; Jackson, C. J.; Tawfik, D. S. Biochemistry 2012, 51, 6047
24. Reeves, T. E.; Wales, M. E.; Grimsley, J. K.; Li, P.; Cerasoli, D. M.; Wild, J. R. Protein Eng. Des. Sel. 2008, 21, 405
25. Schofield, D. A.; DiNovo, A. A. J. Appl. Microbiol. 2010, 109, 548
26. Horton, R. M.; Hunt, H. D.; Ho, S. N.; Pullen, J. K.; Pease, L. R. Gene 1989, 77, 61
27. Goldsmith, M.; Kiss, C.; Bradbury, A. R. M.; Tawfik, D. S. Protein Eng. Des. Sel. 2007, 20, 315
28. Cho, C. M.; Mulchandani, A.; Chen, W. Protein Eng. Des. Sel. 2006, 19, 99
29. Roodveldt, C.; Tawfik, D. S. Protein Eng. Des. Sel. 2005, 18, 51
30. Aubert, S. D.; Li, Y.; Raushel, F. M. Biochemistry 2004, 43, 5707