Icariin induces osteoblast proliferation, differentiation and mineralization through estrogen receptor-mediated ERK and JNK signal activation

European Journal of Pharmacology

Volumn 714, Issue 1-3, 2013, Pages 15-22

  Song, Lige ^a   Zhao, Jiashen ^a   Zhang, Xiuzhen ^a   Li, Hong ^a   Zhou, Yun ^a  

^a TONGJI UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

Estrogen receptor; Icariin; Mitogen activated protein kinases; Osteoblasts; Osteoporosis

Indexed keywords

ALKALINE PHOSPHATASE; CASPASE 3; COLLAGEN TYPE 1; CYCLIN DEPENDENT KINASE INHIBITOR 2B; CYCLIN E; CYCLINE; ESTROGEN RECEPTOR; ICARIIN; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE P38; STRESS ACTIVATED PROTEIN KINASE;

ANIMAL CELL; APOPTOSIS; ARTICLE; CDKN2B GENE; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; CYCLIN E GENE; DRUG STRUCTURE; MINERALIZATION; MOUSE; NONHUMAN; OSSIFICATION; OSTEOBLAST; PCNA GENE; PRIORITY JOURNAL;

ESTROGEN RECEPTOR; ICARIIN; MITOGEN-ACTIVATED PROTEIN KINASES; OSTEOBLASTS; OSTEOPOROSIS;

ANABOLIC AGENTS; ANIMALS; APOPTOSIS; CALCIFICATION, PHYSIOLOGIC; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME ACTIVATION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FEMALE; FLAVONOIDS; HUMANS; JNK MITOGEN-ACTIVATED PROTEIN KINASES; MAP KINASE SIGNALING SYSTEM; MICE; OSTEOBLASTS; RATS; RECEPTORS, ESTROGEN;

EID: 84879478532     PISSN: 00142999     EISSN: 18790712     Source Type: Journal    
DOI: 10.1016/j.ejphar.2013.05.039     Document Type: Article

References (38)

1. Aleem, E., Kiyokawa, H., Kaldis, P., 2005. Cdc2-cyclin E complexes regulate the G1/S phase transition. Nat. Cell Biol. 7, 831-836. (Pubitemid 41079056)
2. Bone, H., 2012. Future directions in osteoporosis therapeutics. Endocrinol. Metab. Clin. North Am. 41, 655-661.
3. Bosetti, M., Leigheb, M., Brooks, R.A., Boccafoschi, F., Cannas, M.F., 2010. Regulation of osteoblast and osteoclast functions by FGF-6. J. Cell Physiol. 225, 466-471.
4. Cao, H., Ke, Y., Zhang, Y., Zhang, C.J., Qian, W., Zhang, G.L., 2012. Icariin stimulates MC3T3-E1 cell proliferation and differentiation through up-regulation of bone morphogenetic protein-2. Int. J. Mol. Med. 29, 435-439.
5. Chau, J.F., Leong, W.F., Li, B., 2009. Signaling pathways governing osteoblast proliferation, differentiation and function. Histol. Histopathol. 24, 1593-1606.
6. Chen, K.M., Ge, B.F., Ma, H.P., Liu, X.Y., Bai, M.H., Wang, Y., 2005. Icariin, a flavonoid from the herb Epimedium enhances the osteogenic differentiation of rat primary bone marrow stromal cells. Pharmazie 60, 939-942. (Pubitemid 41820762)
7. Fulzele, K., DiGirolamo, D.J., Liu, Z., Xu, J., Messina, J.L., Clemens, T.L., 2007. Disruption of the insulin-like growth factor type 1 receptor in osteoblasts enhances insulin signaling and action. J. Biol. Chem. 282, 25649-25658. (Pubitemid 47372844)
8. Guicheux, J., Lemonnier, J., Ghayor, C., Suzuki, A., Palmer, G., Caverzasio, J., 2003. Activation of p38 mitogen-activated protein kinase and c-Jun-NH2-terminal kinase by BMP-2 and their implication in the stimulation of osteoblastic cell differentiation. J. Bone Miner. Res. 18, 2060-2068. (Pubitemid 37377457)
9. Horsch, K., De Wet, H., Schuurmans, M.M., Allie-Reid, F., Cato, A.C., Cunningham, J., Burrin, J.M., Hough, F.S., Hulley, P.A., 2007. Mitogen-activated protein kinase phosphatase 1/dual specificity phosphatase 1 mediates glucocorticoid inhibition of osteoblast proliferation. Mol. Endocrinol. 21, 2929-2940. (Pubitemid 350225576)
10. Hsieh, C.P., Chiou, Y.L., Lin, C.Y., 2010. Hyperbaric oxygen-stimulated proliferation and growth of osteoblasts may be mediated through the FGF-2/MEK/ERK 1/2/NF-kappaB and PKC/JNK pathways. Connect. Tissue Res. 51, 497-509.
11. Hsieh, T.P., Sheu, S.Y., Sun, J.S., Chen, M.H., Liu, M.H., 2010. Icariin isolated from Epimedium pubescens regulates osteoblasts anabolism through BMP-2, SMAD4, and Cbfa1 expression. Phytomedicine 17, 414-423.
12. Hsieh, T.P., Sheu, S.Y., Sun, J.S., Chen, M.H., 2011. Icariin inhibits osteoclast differentiation and bone resorption by suppression of MAPKs/NF-kappaB regulated HIF-1alpha and PGE(2) synthesis. Phytomedicine 18, 176-185.
13. Johnson, G.L., Lapadat, R., 2002. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298, 1911-1912. (Pubitemid 35425238)
14. Jensen, E.D., Gopalakrishnan, R., Westendorf, J.J., 2010. Regulation of gene expression in osteoblasts. Biofactors 36, 25-32.
15. Kong, X.H., Niu, Y.B., Song, X.M., Zhao, D.D., Wang, J., Wu, X.L., Zhang, R., Mei, Q.B., 2012. Astragaloside II induces osteogenic activities of osteoblasts through the bone morphogenetic protein-2/MAPK and Smad1/5/8 pathways. Int. J. Mol. Med. 29, 1090-1098.
16. Lorenzo, J., 2003. A new hypothesis for how sex steroid hormones regulate bone mass. J. Clin. Invest. 111, 1641-1643. (Pubitemid 38057743)
17. Ma, D.F., Qin, L.Q., Wang, P.Y., Katoh, R., 2008. Soy isoflavone intake inhibits bone resorption and stimulates bone formation in menopausal women: meta-analysis of randomized controlled trials. Eur. J. Clin. Nutr. 62, 155-161. (Pubitemid 351234248)
18. Marie, P.J., 2008. Transcription factors controlling osteoblastogenesis. Arch. Bio-chem. Biophys. 473, 98-105.
19. Marino, M., Pellegrini, M., La Rosa, P., Acconcia, F., 2012. Susceptibility of estrogen receptor rapid responses to xenoestrogens: physiological outcomes. Steroids 77, 910-917.
20. Matsuguchi, T., Chiba, N., Bandow, K., Kakimoto, K., Masuda, A., Ohnishi, T., 2009. JNK activity is essential for Atf4 expression and late-stage osteoblast differentiation. J. Bone Miner. Res. 24, 398-410.
21. Matsushita, T., Chan, Y.Y., Kawanami, A., Balmes, G., Landreth, G.E., Murakami, S., 2009. Extracellular signal-regulated kinase 1 (ERK1) and ERK2 play essential roles in osteoblast differentiation and in supporting osteoclastogenesis. Mol. Cell Biol. 29, 5843-5857.
22. Matsumoto, T., Kuriwaka-Kido, R., Kondo, T., Endo, I., Kido, S., 2012. Regulation of osteoblast differentiation by interleukin-11 via AP-1 and Smad signaling. Endocr. J 59, 91-101.
23. Mok, S.K., Chen, W.F., Lai, W.P., Leung, P.C., Wang, X.L., Yao, X.S., Wong, M.S., 2010. Icariin protects against bone loss induced by estrogen deficiency and activates estrogen receptor-dependent osteoblastic functions in UMR 106 cells. Br. J. Pharmacol. 159, 939-949.
24. Nian, H., Ma, M.H., Nian, S.S., Xu, L.L., 2009. Antiosteoporotic activity of icariin in ovariectomized rats. Phytomedicine 16, 320-326.
25. Pan, Y., Wang, F.M., Qiang, L.Q., Zhang, D.M., Kong, L.D., 2010. Icariin attenuates chronic mild stress-induced dysregulation of the LHPA stress circuit in rats. Psychoneuroendocrinology 35, 272-283.
26. Pantouli, E., Boehm, M.M., Koka, S., 2005. Inflammatory cytokines activate p38 MAPK to induce osteoprotegerin synthesis by MG-63 cells. Biochem. Biophys. Res. Commun. 329, 224-229. (Pubitemid 40255444)
27. Rachner, T.D., Khosla, S., Hofbauer, L.C., 2011. Osteoporosis: now and the future. Lancet 377, 1276-1287.
28. Strzalka, W., Ziemienowicz, A., 2011. Proliferating cell nuclear antigen (PCNA): a key factor in DNA replication and cell cycle regulation. Ann. Bot. 107, 1127-1140.
29. Wagner, E.F., 2010. Bone development and inflammatory disease is regulated by AP-1 (Fos/Jun). Ann. Rheum. Dis. 69 (Suppl. 1), i86-88.
30. Wang, X., Goh, C.H., Li, B., 2007. p38 mitogen-activated protein kinase regulates osteoblast differentiation through osterix. Endocrinology 148, 1629-1637. (Pubitemid 46457114)
31. Wo, Y., Zhu, D., Yu, Y., Lou, Y., 2008. Involvement of NF-kappaB and AP-1 activation in icariin promoted cardiac differentiation of mouse embryonic stem cells. Eur. J. Pharmacol 586, 59-66.
32. Wu, R.W., Lin, T.P., Ko, J.Y., Yeh, D.W., Chen, M.W., Ke, H.C., Wu, S.L., Wang, F.S., 2011. Cannabinoid receptor 1 regulates ERK and GSK-3beta-dependent glucocorticoid inhibition of osteoblast differentiation in murine MC3T3-E1 cells. Bone 49, 1255-1263.
33. Xiao, Q., Chen, A., Guo, F., 2005. Effects of Icariin on expression of OPN mRNA and type I collagen in rat osteoblasts in vitro. J. Huazhong Univ. Sci. Technol. Med. Sci. 25, 690-692.
34. Yang, J., Zhang, X., Wang, W., Liu, J., 2010. Insulin stimulates osteoblast proliferation and differentiation through ERK and PI3K in MG-63 cells. Cell Biochem. Funct. 28, 334-341.
35. Yang, S.H., Sharrocks, A.D., Whitmarsh, A.J., 2013. MAP kinase signalling cascades and transcriptional regulation. Gene 513, 1-13.
36. Zaidi, M., Blair, H.C., Iqbal, J., Zhu, L.L., Kumar, T.R., Zallone, A., Sun, L., 2007. Proresorptive actions of FSH and bone loss. Ann. N.Y. Acad. Sci. 1116, 376-382. (Pubitemid 350274338)
37. Zhang, G., Qin, L., Shi, Y., 2007. Epimedium-derived phytoestrogen flavonoids exert beneficial effect on preventing bone loss in late postmenopausal women: a 24-month randomized, double-blind and placebo-controlled trial. J. Bone Miner. Res. 22, 1072-1079. (Pubitemid 351343696)
38. Zhao, J., Ohba, S., Shinkai, M., Chung, U.I., Nagamune, T., 2008. Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner. Biochem. Biophys. Res. Commun. 369, 444-448.