Rapid construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering

Applied Microbiology and Biotechnology

Volumn 97, Issue 13, 2013, Pages 5731-5741

  Lee, Kyoung Ho ^a   Onitsuka, Masayoshi ^b   Honda, Kohsuke ^a   Ohtake, Hisao ^a   Omasa, Takeshi ^a,b  

^a OSAKA UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKUSHIMA   (Japan)

Author keywords

ATR; Chinese hamster ovary (CHO) cells; Gene amplification; Monoclonal antibody

Indexed keywords

ATR; CHINESE HAMSTER OVARY CELLS; GENE AMPLIFICATION; GREEN FLUORESCENT PROTEIN; MONOCLONAL ANTIBODIES (MAB); SMALL INTERFERING RNA (SIRNA); SPECIFIC PRODUCTION RATE; VOLUMETRIC PRODUCTIVITY;

CELL CULTURE; CLONE CELLS; CLONING; GENE EXPRESSION; LAKES; MONOCLONAL ANTIBODIES; PRODUCTIVITY; PROTEINS; RNA;

CELL ENGINEERING;

ATR PROTEIN; GREEN FLUORESCENT PROTEIN; METHOTREXATE; MONOCLONAL ANTIBODY; SMALL INTERFERING RNA;

ANTIBODY; CLONE; GENETIC ENGINEERING; GROWTH RATE; PROTEIN; RODENT;

ANIMAL CELL; ARTICLE; CELL CYCLE CHECKPOINT; CELL ENGINEERING; CHO CELL; CHROMOSOME ABERRATION; COPY NUMBER VARIATION; DOWN REGULATION; GENE AMPLIFICATION; GENE EXPRESSION; NONHUMAN; OVARY CELL; PROTEIN EXPRESSION; TRANSGENE; VOLUMETRY;

ANIMALS; BIOTECHNOLOGY; CELL CYCLE CHECKPOINTS; CELL CYCLE PROTEINS; CHO CELLS; CRICETULUS; GENE EXPRESSION; GENE SILENCING; GENES, REPORTER; GENETIC ENGINEERING; GREEN FLUORESCENT PROTEINS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TRANSGENES;

CHINA;

ATAXIA TELANGIECTASIA; CRICETULUS GRISEUS;

EID: 84879418366     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4923-9     Document Type: Article

References (48)

1. Bi JX, Shuttleworth J, Al-Rubeai M (2004) Uncoupling of cell growth and proliferation results in enhancement of productivity in p21CIP1-arrested CHO cells. Biotechnol Bioeng 85(7):741-749. doi: 10.1002/bit.20025
2. Brummelkamp TR, Bernards R, Agami R (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296(5567):550-553. doi: 10.1126/science.1068999
3. Cacciatore JJ, Chasin LA, Leonard EF (2010) Gene amplification and vector engineering to achieve rapid and high-level therapeutic protein production using the Dhfr-based CHO cell selection system. Biotechnol Adv 28(6):673-681. doi: 10.1016/j.biotechadv.2010.04.003
4. Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T (2012a) Construction of BAC-based physical map and analysis of chromosome rearrangement in Chinese hamster ovary cell lines. Biotechnol Bioeng 109(6):1357-1367. doi: 10.1002/bit.24347
5. Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T (2012b) Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells. Methods 56:418-423. doi: 10.1016/j.ymeth.2011.11.002
6. Casper AM, Nghiem P, Arlt MF, Glover TW (2002) ATR regulates fragile site stability. Cell 111(6):779-789
7. Chuck AS, Palsson BO (1992) Population balance between producing and nonproducing hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition. Biotechnol Bioeng 39(3):354-360. doi: 10.1002/bit.260390315
8. Chung JY, Lim SW, Hong YJ, Hwang SO, Lee GM (2004) Effect of doxycycline-regulated calnexin and calreticulin expression on specific thrombopoietin productivity of recombinant Chinese hamster ovary cells. Biotechnol Bioeng 85(5):539-546. doi: 10.1002/bit.10919
9. Cimprich KA, Cortez D (2008) ATR: an essential regulator of genome integrity. Nat Rev Mol Cell Biol 9(8):616-627. doi: 10.1038/nrm2450
10. Coquelle A, Pipiras E, Toledo F, Buttin G, Debatisse M (1997) Expression of fragile sites triggers intrachromosomal mammalian gene amplification and sets boundaries to early amplicons. Cell 89(2):215-225
11. Durkin SG, Glover TW (2007) Chromosome fragile sites. Annu Rev Genet 41:169-192. doi: 10.1146/annurev.genet.41.042007.165900
12. Gandor C, Leist C, Fiechter A, Asselbergs FA (1995) Amplification and expression of recombinant genes in serum-independent Chinese hamster ovary cells. FEBS Lett 377(3):290-294. doi: 10.1016/0014-5793(95)01328-8
13. Glover TW, Berger C, Coyle J, Echo B (1984) DNA polymerase alpha inhibition by aphidicolin induces gaps and breaks at common fragile sites in human chromosomes. Hum Genet 67(2):136-142
14. Glover TW, Coyle-Morris J, Morgan R (1986) Fragile sites: overview, occurrence in acute nonlymphocytic leukemia and effects of caffeine on expression. Cancer Genet Cytogenet 19(1-2):141-150
15. Guarna MM, Fann CH, Busby SJ, Walker KM, Kilburn DG, Piret JM (1995) Effect of cDNA copy number on secretion rate of activated protein C. Biotechnol Bioeng 46(1):22-27. doi: 10.1002/bit.260460104
16. Gupta S, Schoer RA, Egan JE, Hannon GJ, Mittal V (2004) Inducible, reversible, and stable RNA interference in mammalian cells. Proc Natl Acad Sci U S A 101(7):1927-1932. doi: 10.1073/pnas.0306111101
17. Hammond S, Swanberg JC, Kaplarevic M, Lee KH (2011) Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology. BMC Genomics 12:67. doi: 10.1186/1471-2164-12-67
18. Jeon MK, Yu DY, Lee GM (2011) Combinatorial engineering of ldh-a and bcl-2 for reducing lactate production and improving cell growth in dihydrofolate reductase-deficient Chinese hamster ovary cells. Appl Microbiol Biotechnol 92(4):779-790. doi: 10.1007/s00253-011-3475-0
19. Jiang Z, Huang Y, Sharfstein ST (2006) Regulation of recombinant monoclonal antibody production in chinese hamster ovary cells: a comparative study of gene copy number, mRNA level, and protein expression. Biotechnol Prog 22(1):313-318. doi: 10.1021/bp0501524
20. Kappel S, Matthess Y, Kaufmann M, Strebhardt K (2007) Silencing of mammalian genes by tetracycline-inducible shRNA expression. Nat Protoc 2(12):3257-3269. doi: 10.1038/nprot.2007.458
21. Kaufman RJ (1990) Selection and coamplification of heterologous genes in mammalian cells. Methods Enzymol 185:537-566
22. Kuystermans D, Al-Rubeai M (2009) cMyc increases cell number through uncoupling of cell division from cell size in CHO cells. BMC Biotechnol 9:76. doi: 10.1186/1472-6750-9-76
23. Kim JY, Kim YG, Lee GM (2012) CHO cells in biotechnology for production of recombinant proteins: current state and further potential. Appl Microbiol Biotechnol 93(3):917-930. doi: 10.1007/s00253-011-3758-5
24. Kim WD, Tokunaga M, Ozaki H, Ishibashi T, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Omasa T, Ohtake H (2010) Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells. Appl Microbiol Biotechnol 85:535-542. doi: 10.1007/s00253-009-2152-z
25. Lukusa T, Fryns JP (2008) Human chromosome fragility. Biochim Biophys Acta 1779(1):3-16. doi: 10.1016/j.bbagrm.2007.10.005
26. Ma C, Martin S, Trask B, Hamlin JL (1993) Sister chromatid fusion initiates amplification of the dihydrofolate reductase gene in Chinese hamster cells. Genes Dev 7(4):605-620
27. Majors BS, Arden N, Oyler GA, Chiang GG, Pederson NE, Betenbaugh MJ (2008) E2F-1 overexpression increases viable cell density in batch cultures of Chinese hamster ovary cells. J Biotechnol 138(3-4):103-106. doi: 10.1016/j.jbiotec.2008.08.003
28. Martins MB, Sabatier L, Ricoul M, Pinton A, Dutrillaux B (1993) Specific chromosome instability induced by heavy ions: a step towards transformation of human fibroblasts? Mutat Res 285(2):229-237
29. Mohan C, Park SH, Chung JY, Lee GM (2007) Effect of doxycycline-regulated protein disulfide isomerase expression on the specific productivity of recombinant CHO cells: thrombopoietin and antibody. Biotechnol Bioeng 98(3):611-615. doi: 10.1002/bit.21453
30. Musio A, Montagna C, Mariani T, Tilenni M, Focarelli ML, Brait L, Indino E, Benedetti PA, Chessa L, Albertini A, Ried T, Vezzoni P (2005) SMC1 involvement in fragile site expression. Hum Mol Genet 14(4):525-533. doi: 10.1093/hmg/ddi049
31. Ng SK, Wang DI, Yap MG (2007) Application of destabilizing sequences on selection marker for improved recombinant protein productivity in CHO-DG44. Metab Eng 9(3):304-316. doi: 10.1016/j.ymben.2007.01.001
32. O'Callaghan PM, James DC (2008) Systems biotechnology of mammalian cell factories. Brief Funct Genomic Proteomic 7(2):95-110. doi: 10.1093/bfgp/eln012
33. Ohya T et al (2008) Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression. Biotechnol Bioeng 100(2):317-324. doi: 10.1002/Bit.21758
34. Omasa T (2002) Gene amplification and its application in cell and tissue engineering. J Biosci Bioeng 94(6):600-605
35. Omasa T, Higashiyama K, Shioya S, Suga K (1992) Effects of lactate concentration on hybridoma culture in lactate-controlled fed-batch operation. Biotechnol Bioeng 39(5):556-564
36. Onitsuka M, Kim WD, Ozaki H, Kawaguchi A, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Ohtake H, Omasa T (2012) Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of alpha2,6- sialyltransferase derived from Chinese hamster ovary cells. Appl Microbiol Biotechnol 94:69-80. doi: 10.1007/s00253-011-3814-1
37. Park JY, Yamatani M, Wadano S, Takagi Y, Honda K, Omasa T, Ohtake H (2010) Effects of palindrome structure on Dhfr amplification in Chinese hamster ovary cells. Process Biochem 45(12):1845-1851. doi: 10.1016/j.procbio.2009.11. 019
38. Pendse GJ, Karkare S, Bailey JE (1992) Effect of cloned gene dosage on cell growth and hepatitis B surface antigen synthesis and secretion in recombinant CHO cells. Biotechnol Bioeng 40(1):119-129. doi: 10.1002/bit.260400117
39. Peroni CN, Soares CR, Gimbo E, Morganti L, Ribela MT, Bartolini P (2002) High-level expression of human thyroid-stimulating hormone in Chinese hamster ovary cells by co-transfection of dicistronic expression vectors followed by a dual-marker amplification strategy. Biotechnol Appl Biochem 35(Pt 1):19-26
40. Roobol A, Roobol J, Carden MJ, Bastide A, Willis AE, Dunn WB, Goodacre R, Smales CM (2011) ATR (ataxia telangiectasia mutated- and Rad3-related kinase) is activated by mild hypothermia in mammalian cells and subsequently activates p53. Biochem J 435(2):499-508. doi: 10.1042/BJ20101303
41. Suzuki E, Ollis DF (1989) Cell cycle model for antibody production kinetics. Biotechnol Bioeng 34(11):1398-1402. doi: 10.1002/bit.260341109
42. Urlaub G, Chasin LA (1980) Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci U S A 77(7):4216-4220
43. Wurm FM (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22(11):1393-1398. doi: 10.1038/nbt1026
44. Xiong KH, Liang QC, Xiong H, Zou CX, Gao GD, Zhao ZW, Zhang H (2005) Expression of chimeric antibody in mammalian cells using dicistronic expression vector. Biotechnol Lett 27(21):1713-1717. doi: 10.1007/s10529-005-2736-3
45. Xu X, Nagarajan H, Lewis NE, Pan S, Cai Z, Liu X, Chen W, Xie M, Wang W, Hammond S, Andersen MR, Neff N, Passarelli B, Koh W, Fan HC, Wang J, Gui Y, Lee KH, Betenbaugh MJ, Quake SR, Famili I, Palsson BO (2011) The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nat Biotechnol 29(8):735-741. doi: 10.1038/nbt.1932
46. Yoshikawa T, Nakanishi F, Itami S, Kameoka D, Omasa T, Katakura Y, Kishimoto M, Suga K (2000a) Evaluation of stable and highly productive gene amplified CHO cell line based on the location of amplified genes. Cytotechnology 33(1-3):37-46. doi: 10.1023/A:1008111328771
47. Yoshikawa T, Nakanishi F, Ogura Y, Oi D, Omasa T, Katakura Y, Kishimoto M, Suga K (2000b) Amplified gene location in chromosomal DNA affected recombinant protein production and stability of amplified genes. Biotechnol Prog 16(5):710-715. doi: 10.1021/bp000114e
48. Yoshikawa T, Nakanishi F, Ogura Y, Oi D, Omasa T, Katakura Y, Kishimoto M, Suga KI (2001) Flow cytometry: an improved method for the selection of highly productive gene-amplified CHO cells using flow cytometry. Biotechnol Bioeng 74(5):435-442. doi: 10.1002/bit.1134