Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α2,6-sialyltransferase derived from Chinese hamster ovary cells

Applied Microbiology and Biotechnology

Volumn 94, Issue 1, 2012, Pages 69-80

  Onitsuka, Masayoshi ^a   Kim, Wook Dong ^b   Ozaki, Hiroyuki ^b   Kawaguchi, Akira ^b   Honda, Kohsuke ^b   Kajiura, Hiroyuki ^b   Fujiyama, Kazuhito ^b   Asano, Ryutaro ^c   Kumagai, Izumi ^c   Ohtake, Hisao ^b   Omasa, Takeshi ^a,b  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c TOHOKU UNIVERSITY   (Japan)

Author keywords

Antibody production; Beta galactosyl alpha 2,6 sialyltransferase (ST6Gal I); Bispecific diabody; Chinese hamster ovary (CHO) cell; Glycosylation control; Sialylation

Indexed keywords

ANTIBODY PRODUCTION; BETA-GALACTOSYL ALPHA-2,6 SIALYLTRANSFERASE (ST6GAL I); BISPECIFIC DIABODY; CHINESE HAMSTER OVARY CELLS; SIALYLATION;

CELL CULTURE; CELLS; CLONING; ESCHERICHIA COLI; ESTERIFICATION; GLYCOSYLATION;

ANTIBODIES;

BETA GALACTOSYL ALPHA 2,6 SIALYLTRANSFERASE; BISPECIFIC ANTIBODY; COMPLEMENTARY DNA; HUMAN IMMUNOGLOBULIN; IMMUNOGLOBULIN G; OLIGOSACCHARIDE; RECOMBINANT ANTIBODY; SIALIDASE; SIALYLTRANSFERASE; UNCLASSIFIED DRUG;

ANTIBODY; BIOASSAY; CLONE; CYTOLOGY; ENZYME ACTIVITY; GENE EXPRESSION; LIQUID CHROMATOGRAPHY;

ANIMAL CELL; ANIMAL TISSUE; ANTIINFLAMMATORY ACTIVITY; ARTICLE; BINDING ASSAY; CELL LINE; CHO CELL; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPRESSION VECTOR; FEMALE; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; IN VITRO GENE TRANSFER; INDUSTRIAL PRODUCTION; LECTIN BINDING; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; RAT; SIALYLATION;

ANIMALS; ANTIBODIES, BISPECIFIC; ANTIBODIES, MONOCLONAL, HUMANIZED; CHO CELLS; CRICETINAE; ESCHERICHIA COLI; FEMALE; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN G; OVARY; RECOMBINANT PROTEINS; SIALYLTRANSFERASES; UP-REGULATION;

CRICETULUS GRISEUS; ESCHERICHIA COLI;

EID: 84858441807     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3814-1     Document Type: Article

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