Epidermal keratinocytes form a functional skin barrier in the absence of Atg7 dependent autophagy

Journal of Dermatological Science

Volumn 71, Issue 1, 2013, Pages 67-75

  Rossiter, Heidemarie ^a   König, Ulrich ^a   Barresi, Caterina ^a   Buchberger, Maria ^a   Ghannadan, Minoo ^a   Zhang, Cheng Feng ^a,b   Mlitz, Veronika ^a   Gmeiner, Ramona ^a   Sukseree, Supawadee ^a,c   Födinger, Dagmar ^a   Eckhart, Leopold ^a   Tschachler, Erwin ^a  

^a Universitatsklinik fur Dermatologie   (Austria)

^b FUDAN UNIVERSITY   (China)

^c SRINAKHARINWIROT UNIVERSITY   (Thailand)

Author keywords

Autophagy; Epidermis; GFP LC3; Keratinocytes

Indexed keywords

CELL PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; AUTOPHAGOSOME; AUTOPHAGY; AUTOPHAGY ASSOCIATED GENE 7; CELL FUNCTION; CONTROLLED STUDY; EPIDERMIS; GENE; GENE INACTIVATION; HISTOLOGY; KERATINOCYTE; MEMBRANE FORMATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SKIN FUNCTION; SKIN STRUCTURE; SKIN WATER LOSS; WESTERN BLOTTING;

ANIMALS; AUTOPHAGY; CELL DIFFERENTIATION; CELLS, CULTURED; DIFFUSION; EPIDERMIS; GREEN FLUORESCENT PROTEINS; KERATIN-14; KERATINOCYTES; MICE; MICE, INBRED CBA; MICE, KNOCKOUT; MICE, TRANSGENIC; MICROTUBULE-ASSOCIATED PROTEINS; PERMEABILITY; PROMOTER REGIONS, GENETIC; SKIN ABSORPTION; WATER LOSS, INSENSIBLE;

EID: 84879031086     PISSN: 09231811     EISSN: 1873569X     Source Type: Journal    
DOI: 10.1016/j.jdermsci.2013.04.015     Document Type: Article

References (43)

1. Candi E., Schmidt R., Melino G. The cornified envelope: a model of cell death in the skin. Nat Rev Mol Cell Biol 2005, 6:328-340.
2. Elias P.M. Stratum corneum defensive functions: an integrated view. J Invest Dermatol 2005, 125:183-200.
3. He C., Klionsky D.J. Regulation mechanisms and signaling pathways of autophagy. Annu Rev Genet 2009, 43:67-93.
4. Komatsu M., Waguri S., Koike M., Sou Y.S., Ueno T., Hara T., et al. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 2007, 131:1149-1163.
5. Choi A.M., Ryter S.W., Levine B. Autophagy in human health and disease. N Engl J Med 2013, 368:651-662.
6. Mizushima N., Komatsu M. Autophagy: renovation of cells and tissues. Cell 2011, 147:728-741.
7. Komatsu M., Waguri S., Ueno T., Iwata J., Murata S., Tanida I., et al. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol 2005, 169:425-434.
8. Kuma A., Hatano M., Matsui M., Yamamoto A., Nakaya H., Yoshimori T., et al. The role of autophagy during the early neonatal starvation period. Nature 2004, 432:1032-1036.
9. Hara T., Nakamura K., Matsui M., Yamamoto A., Nakahara Y., Suzuki-Migishima R., et al. Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice. Nature 2006, 441:885-889.
10. Komatsu M., Waguri S., Chiba T., Murata S., Iwata J., Tanida I., et al. Loss of autophagy in the central nervous system causes neurodegeneration in mice. Nature 2006, 441:880-884.
11. Masiero E., Agatea L., Mammucari C., Blaauw B., Loro E., Komatsu M., et al. Autophagy is required to maintain muscle mass. Cell Metab 2009, 10:507-515.
12. Ebato C., Uchida T., Arakawa M., Komatsu M., Ueno T., Komiya K., et al. Autophagy is important in islet homeostasis and compensatory increase of beta cell mass in response to high-fat diet. Cell Metab 2008, 8:325-332.
13. Jung H.S., Chung K.W., Won Kim J., Kim J., Komatsu M., Tanaka K., et al. Loss of autophagy diminishes pancreatic beta cell mass and function with resultant hyperglycemia. Cell Metab 2008, 8:318-324.
14. Mizushima N., Levine B. Autophagy in mammalian development and differentiation. Nat Cell Biol 2010, 12:823-830.
15. Haruna K., Suga Y., Muramatsu S., Taneda K., Mizuno Y., Ikeda S., et al. Differentiation-specific expression and localization of an autophagosomal marker protein (LC3) in human epidermal keratinocytes. J Dermatol Sci 2008, 52:213-215.
16. Aymard E., Barruche V., Naves T., Bordes S., Closs B., Verdier M., et al. Autophagy in human keratinocytes: an early step of the differentiation?. Exp Dermatol 2011, 20:263-268.
17. Wang R.C., Levine B. Calcipotriol induces autophagy in HeLa cells and keratinocytes. J Invest Dermatol 2011, 131:990-993.
18. Lee H.M., Shin D.M., Yuk J.M., Shi G., Choi D.K., Lee S.H., et al. Autophagy negatively regulates keratinocyte inflammatory responses via scaffolding protein p62/SQSTM1. J Immunol 2011, 186:1248-1258.
19. Mizushima N., Yamamoto A., Matsui M., Yoshimori T., Ohsumi Y. In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker. Mol Biol Cell 2004, 15:1101-1111.
20. Rossiter H., Barresi C., Ghannadan M., Gruber F., Mildner M., Fodinger D., et al. Inactivation of VEGF in mammary gland epithelium severely compromises mammary gland development and function. FASEB J 2007, 21:3994-4004.
21. Lippens S., Kockx M., Knaapen M., Mortier L., Polakowska R., Verheyen A., et al. Epidermal differentiation does not involve the pro-apoptotic executioner caspases, but is associated with caspase-14 induction and processing. Cell Death Differ 2000, 7:1218-1224.
22. Marino G., Salvador-Montoliu N., Fueyo A., Knecht E., Mizushima N., Lopez-Otin C. Tissue-specific autophagy alterations and increased tumorigenesis in mice deficient in Atg4C/autophagin-3. J Biol Chem 2007, 282:18573-18583.
23. Rendl M., Ban J., Mrass P., Mayer C., Lengauer B., Eckhart L., et al. Caspase-14 expression by epidermal keratinocytes is regulated by retinoids in a differentiation-associated manner. J Invest Dermatol 2002, 119:1150-1155.
24. Swierenga S.H.H., MacManus J.P. Preparation of low calcium growth medium suitable for determination of tumorigenicity of cultured cells. J Tissue Cult Methods 1982, 7:1-3.
25. Caldelari R., Suter M.M., Baumann D., de B.A., Muller E. Long-term culture of murine epidermal keratinocytes. J Invest Dermatol 2000, 114:1064-1065.
26. Koch P.J., de Viragh P.A., Scharer E., Bundman D., Longley M.A., Bickenbach J., et al. Lessons from loricrin-deficient mice: compensatory mechanisms maintaining skin barrier function in the absence of a major cornified envelope protein. J Cell Biol 2000, 151:389-400.
27. List K., Haudenschild C.C., Szabo R., Chen W., Wahl S.M., Swaim W., et al. Matriptase/MT-SP1 is required for postnatal survival, epidermal barrier function, hair follicle development, and thymic homeostasis. Oncogene 2002, 21:3765-3779.
28. Hardman M.J., Sisi P., Banbury D.N., Byrne C. Patterned acquisition of skin barrier function during development. Development 1998, 125:1541-1552.
29. Mizushima N., Yoshimori T., Levine B. Methods in mammalian autophagy research. Cell 2010, 140:313-326.
30. Ni H.M., Bockus A., Wozniak A.L., Jones K., Weinman S., Yin X.M., et al. Dissecting the dynamic turnover of GFP-LC3 in the autolysosome. Autophagy 2011, 7:188-204.
31. Eckhart L., Declercq W., Ban J., Rendl M., Lengauer B., Mayer C., et al. Terminal differentiation of human keratinocytes and stratum corneum formation is associated with caspase-14 activation. J Invest Dermatol 2000, 115:1148-1151.
32. Fischer H., Stichenwirth M., Dockal M., Ghannadan M., Buchberger M., Bach J., et al. Stratum corneum-derived caspase-14 is catalytically active. FEBS Lett 2004, 577:446-450.
33. Bjorkoy G., Lamark T., Brech A., Outzen H., Perander M., Overvatn A., et al. p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death. J Cell Biol 2005, 171:603-614.
34. Schäfer M., Farwanah H., Willrodt A.H., Huebner A.J., Sandhoff K., Roop D., et al. Nrf2 links epidermal barrier function with antioxidant defense. EMBO Mol Med 2012, 4:364-379.
35. Mortensen M., Ferguson D.J., Edelmann M., Kessler B., Morten K.J., Komatsu M., et al. Loss of autophagy in erythroid cells leads to defective removal of mitochondria and severe anemia in vivo. Proc Natl Acad Sci USA 2010, 107:832-837.
36. Pua H.H., Guo J., Komatsu M., He Y.W. Autophagy is essential for mitochondrial clearance in mature T lymphocytes. J Immunol 2009, 182:4046-4055.
37. Mizushima N., Kuma A. Autophagosomes in GFP-LC3 transgenic mice. Methods Mol Biol 2008, 445:119-124.
38. Sukseree S., Mildner M., Rossiter H., Pammer J., Zhang C.-F., Watanapokasin R., et al. Autophagy in the thymic epithelium is dispensable for the development of self-tolerance in a novel mouse model. PLoS ONE 2012, 7:e38933.
39. Machado M., Hadgraft J., Lane M.E. Assessment of the variation of skin barrier function with anatomic site, age, gender and ethnicity. Int J Cosmet Sci 2010, 32:397-409.
40. Wittkopf N., Gunther C., Martini E., Waldner M., Amann K.U., Neurath M.F., et al. Lack ofintestinal epithelial Atg7 affects paneth cell granule formation but does not compromise immune homeostasis in the gut. Clin Dev Immunol 2012, Article ID: 278059.
41. Fujishima Y., Nishiumi S., Masuda A., Inoue J., Nguyen N.M., Irino Y., et al. Autophagy in the intestinal epithelium reduces endotoxin-induced inflammatory responses by inhibiting NF-kappaB activation. Arch Biochem Biophys 2011, 506:223-235.
42. Zhao Y., Zhang C.F., Rossiter H., Eckhart L., Konig U., Karner S., et al. Autophagy is induced by UVA and promotes removal of oxidized phospholipids and protein aggregates in epidermal keratinocytes. J Invest Dermatol 2013, 10.1038/jid.2013.26.
43. Nishida Y., Arakawa S., Fujitani K., Yamaguchi H., Mizuta T., Kanaseki T., et al. Discovery of Atg5/Atg7-independent alternative macroautophagy. Nature 2009, 461:654-658.