Determination of viable Salmonellae from potable and source water through PMA assisted qPCR

Ecotoxicology and Environmental Safety

Volumn 93, Issue , 2013, Pages 121-127

  Singh, Gulshan ^a   Vajpayee, Poornima ^a   Bhatti, Saurabh ^a   Ronnie, Nirmala ^b   Shah, Nimish ^b   McClure, Peter ^b   Shanker, Rishi ^a  

^a CSIR INDIAN INSTITUTE OF TOXICOLOGY RESEARCH   (India)

^b Hindustan Lever Limited   (India)

Author keywords

Propidium monoazide assisted qPCR; Source and potable water; Viable salmonellae detection in low number

Indexed keywords

DRINKING WATER; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE; UNCLASSIFIED DRUG;

BACTERIUM; DNA; DRINKING WATER; PATHOGENICITY; RISK ASSESSMENT; WATER MANAGEMENT; WATER POLLUTION; WATER QUALITY; WATER SUPPLY;

ARTICLE; BACTERIAL COUNT; BACTERIAL GENE; BACTERIAL VIABILITY; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA CROSS LINKING; INDIA; NONHUMAN; POLYMERASE CHAIN REACTION; SALMONELLA; TTR GENE; URBAN AREA; WATER CONTAMINATION; WATER SUPPLY;

AZIDES; DRINKING WATER; MICROBIAL VIABILITY; PROPIDIUM; REAL-TIME POLYMERASE CHAIN REACTION; SALMONELLA; WATER; WATER MICROBIOLOGY;

INDIA;

SALMONELLA;

EID: 84878548485     PISSN: 01476513     EISSN: 10902414     Source Type: Journal    
DOI: 10.1016/j.ecoenv.2013.02.017     Document Type: Article

References (37)

1. Bae S., Wuertz S. Discrimination of viable and dead fecal Bacteroidales bacteria by quantitative PCR with propidium monoazide. Appl. Environ. Microbiol. 2009, 75:2940-2944.
2. Bae S., Wuertz S. Survival of host-associated Bacteroidales cells and their relationship with Enterococcus spp., Campylobacter jejuni, Salmonella enteric Serovar Typhimurium, and adenovirus in freshwater microcosms as measured by propidium monoazide-quantitative PCR. Appl. Environ. Microbiol. 2012, 78:922-932.
3. Bahl R., Sinha A., Poulos C., Whittington D., Sazawal S., Kumar R., Mahalanabis D., Acosta C.J., Clemens J.D., Bhan M.K. Costs of illness due to typhoid fever in an Indian urban slum community: implications for vaccination policy. J. Health Popul. Nutr. 2004, 22:304-310.
4. Chen S., Wang F., Beaulieu J.C., Stein R.E., Ge B. Rapid detection of viable salmonellae in produce by coupling propidium monoazide with loop-mediated isothermal amplification. Appl. Environ. Microbiol. 2011, 77:4008-4016.
5. Clever M., Jordt F., Knauf R., Räbiger N., Rüdebusch M., Hilker-Scheibel R. Process water production from river water by ultra filtration and reverse osmosis. Desalination 2000, 131:325-336.
6. Garcia T. Simultaneous detection and enumeration of viable lactic acid bacteria and Bifidobacteria in fermented milk by using propidium monoazide and real time PCR. Int. Dairy J. 2009, 19:405-409.
7. Gomez K.A., Gomez A.A. Statistical Procedures for Agricultural Research 1984, Wiley, New York.
8. Hadjinicolaou A.V., Demetriou V.L., Emmanuel M.A., Kakoyiannis C.K., Kostrikis L.G. Molecular beacon-based real-time PCR detection of primary isolates of Salmonella Typhimurium and Salmonella Enteritidis in environmental and clinical samples. BMC Microbiol. 2009, 9:97.
9. Hensel M., Hinsley A.P., Nikolaus T., Sawers G., Berks B.C. The genetic basis of tetrathionate respiration in Salmonella Typhimurium. Mol. Microbiol. 1999, 32:275-287.
10. Josefsen M.H., Krause M., Hansen F., Hoorfar J. Optimization of a 12-h TaqMan PCR-based method for detection of Salmonella bacteria in meat. Appl. Environ. Microbiol. 2007, 73:3040-3048.
11. Jyoti A., Ram S., Vajpayee P., Singh G., Dwivedi P.D., Jain S.K., Shanker R. Contamination of surface and potable water in South Asia by Salmonellae: culture-independent quantification with molecular beacon real-time PCR. Sci. Total Environ. 2010, 408:1256-1263.
12. Jyoti A., Vajpayee P., Singh G., Patel C.B., Gupta K.C., Shanker R. Identification of environmental reservoirs of nontyphoidal salmonellosis: aptamer-assisted bioconcentration and subsequent detection of Salmonella Typhimurium by quantitative polymerase chain reaction. Environ. Sci. Technol. 2011, 45:8996-9002.
13. Levantesi C., Bonadonna L., Briancesco R., Grohmann E., Toze S., Tandoi V. Salmonella in surface and drinking water: occurrence and water-mediated transmission. Food Res. Int. 2012, 45:587-602.
14. Liang N., Dong J., Luo L., Li Y. Detection of viable Salmonella in lettuce by propidium monoazide real-time PCR. J. Food Sci. 2011, 76:M234-7.
15. Li B., Chen J.-Q. Real-time PCR methodology for selective detection of viable Escherichia coli O157:H7 cells by targeting Z3276 as a genetic marker. Appl. Environ. Microbiol. 2012, 78:5297-5304.
16. Lu Y., Yang W., Shi L., Li L., Alam M.J., Guo S., Miyoshi S.-I. Specific detection of viable Salmonella cells by an ethidium monoazide-loop mediated isothermal amplification (EMA-LAMP). J. Health Sci. 2009, 55:820-824.
17. Malorny B., Paccassoni E., Fach P., Bunge C., Martin A., Helmuth R. Diagnostic real-time PCR for detection of Salmonella in food. Appl. Environ. Microbiol. 2004, 70:7046-7052.
18. Malorny B., Bunge C., Helmuth R. A real-time PCR for the detection of Salmonella Enteritidis in poultry meat and consumption eggs. J. Microbiol. Methods 2007, 70:245-251.
19. Malorny B., Löfström C., Wagner M., Krämer N, Hoorfar J. Enumeration of Salmonella bacteria in food and feed samples by real-time PCR for quantitative microbial risk assessment. Appl. Environ. Microbiol. 2008, 74:1299-1304.
20. Martinez U.J., Liebana E., Garcia M.L., Perez P.P., Saco M. Characterization of Salmonella enterica Serovar Typhimurium from marine environments in coastal waters of Galicia (Spain). Appl. Environ. Microbiol. 2004, 70:4030-4034.
21. Mikkelson B.J., Lee, C.M., Ponce, A., 2011. Optimization of PMA-PCR Protocol for Viability Detection of Pathogens. NASA USRP-Internship Final Report, Summer Session. pp. 1-7.
22. Nocker A., Camper A.K. Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl. Environ. Microbiol. 2006, 72:1997-2004.
23. Nocker A., Camper A.K. Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiol. Lett. 2009, 291:137-142.
24. Nocker A., Cheung C.Y., Camper A.K. Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J. Microbiol. Methods 2006, 67:310-320.
25. Nocker A., Sossa K., Camper A.K. Molecular monitoring of disinfection efficacy. J. Microbiol. Methods 2007, 70:252-260.
26. Ochiai R.L., Acosta C.J., Danovaro-Holliday M.C., Baiqing D., Bhattacharya S.K., Agtini M.D., Bhutta Z.A., Canh do G., Ali M., Shin S., Wain J., Page A.L., Albert M.J., Farrar J., Abu-Elyazeed R., Pang T., Galindo C.M., von Seidlein L., Clemens J.D. A study of typhoid fever in five Asian countries: disease burden and implications for controls. Bull. World Health Organ. 2008, 86:260-268. Domi. Typhoid Study Group.
27. Patel C.B., Vajpayee P., Singh G., Upadhyay R.S., Shanker R. Contamination of potable water by enterotoxigenic Escherichia coli: qPCR based culture-free detection and quantification. Ecotoxicol. Environ. Saf. 2011, 74:2292-2298.
28. Ram S., Vajpayee P., Shanker R. Contamination of potable water distribution systems by multi antimicrobial-resistant enterohemorrhagic Escherichia coli. Environ. Health Perspect. 2008, 116:448-452.
29. Ram S., Vajpayee P., Shanker R. Rapid culture-independent quantitative detection of enterotoxigenic Escherichia coli in surface waters by real-time PCR with molecular beacon. Environ. Sci. Technol. 2008, 42:4577-4582.
30. Schets F.M., During M., Italiaander R, Heijnen L., Rutjes S.A., van der Zwaluw W.K., de Roda Husman A.M. Escherichia coli O157: H7 in drinking water from private water supplies in the Netherlands. Water Res. 2005, 39:4485-4493.
31. Singh G., Vajpayee P., Ram S., Shanker R. Environmental reservoirs for enterotoxigenic Escherichia coli in South Asian Gangetic Riverine System. Environ. Sci. Technol. 2010, 44:6475-6480.
32. Setti I., Rodriguez-Castro A., Pata M.P., Cadarso-Suarez C., Yacoubi B., Bensmael L., Moukrim A., Martinez-Urtaza J. Characteristics and dynamics of Salmonella contamination along the coast of Agadir, Morocco. Appl. Environ. Microbiol. 2009, 75:7700-7709.
33. Singh G., Vajpayee P., Khatoon I., Jyoti A., Dhawan A., Gupta K.C. Chromium oxide nano-particles induce stress in bacteria: probing cell viability. J. Biomed. Nanotechnol. 2011, 7:166-167.
34. Singh G., Vajpayee P., Rani N., Jyoti A., Gupta KC., Shanker R. Bio-capture of S. Typhimurium from surface water by aptamer for culture-free quantification. Ecotoxicol. Environ. Saf. 2012, 78:320-326.
35. Taskin B., Gozen A.G., Duran M. Selective quantification of viable Escherichia coli bacteria in biosolids by quantitative PCR with propidium monoazide modification. Appl. Environ. Microbiol. 2011, 77:4329-4335.
36. Voetsch A.C., Gilder T.J.V., Angulo F.J., Farley M.M., Shallow S., Marcus R. Food net estimate of the burden of illness caused by non-typhoidal Salmonella infections in the United States. Clin. Infect. Dis. 2004, 38:27-34.
37. Wagner A.O., Malin C., Knapp B.A., Illmer P. Removal of free extracellular DNA from environmental samples by ethidium monoazide and propidium monoazide. Appl. Environ Microbiol. 2008, 74:2537-2539.