Contamination of surface and potable water in South Asia by Salmonellae: Culture-independent quantification with molecular beacon real-time PCR

Science of the Total Environment

Volumn 408, Issue 6, 2010, Pages 1256-1263

  Jyoti, Anurag ^a   Ram, Siya ^a   Vajpayee, Poornima ^a   Singh, Gulshan ^a   Dwivedi, Premendra D ^a   Jain, Swatantra K ^b   Shanker, Rishi ^a  

^a CSIR INDIAN INSTITUTE OF TOXICOLOGY RESEARCH   (India)

^b HAMDARD UNIVERSITY   (India)

Author keywords

Culture independent; Molecular Beacon; Quantitative enumeration; Real time PCR; Salmonella spp.; Surface and potable water

Indexed keywords

CONSERVED REGIONS; DETECTION PROBABILITIES; E. COLI; ESSENTIAL COMPONENT; MOLECULAR BEACON; NATURAL WATER RESOURCES; PATHOGENIC E. COLI; PROTEIN SECRETION; PUBLIC HEALTH RISKS; QUANTITATIVE ENUMERATION; REAL-TIME PCR; REAL-TIME PCR ASSAY; REFERENCE STRAINS; S. TYPHIMURIUM; SALMONELLA SPP; SALMONELLA TYPHIMURIUM; SEROTYPES; SOUTH ASIA;

ESCHERICHIA COLI; GENE ENCODING; HEALTH RISKS; REAL TIME SYSTEMS; SALMONELLA; WATER DISTRIBUTION SYSTEMS; WATER POLLUTION; WATER RESOURCES; WATER SUPPLY;

POTABLE WATER;

BACTERIAL PROTEIN; DRINKING WATER; PROTEIN INVA; SURFACE WATER; UNCLASSIFIED DRUG;

BIOASSAY; COLIFORM BACTERIUM; DRINKING WATER; HEALTH RISK; POLYMERASE CHAIN REACTION; SURFACE WATER; WATER POLLUTION;

ARTICLE; BACTERIAL COUNT; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; COLONY FORMING UNIT; CONTROLLED STUDY; ESCHERICHIA COLI; INDIA; MOLECULAR BEACON; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SECRETION; PUBLIC HEALTH; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA; SALMONELLA TYPHIMURIUM; WATER CONTAMINATION; WATER SUPPLY;

BACTERIAL PROTEINS; COLONY COUNT, MICROBIAL; ENVIRONMENTAL MONITORING; FRESH WATER; INDIA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SALMONELLA; SENSITIVITY AND SPECIFICITY; WATER POLLUTION;

INDIA;

SALMONELLA; SALMONELLA TYPHIMURIUM;

EID: 74849103914     PISSN: 00489697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.scitotenv.2009.11.056     Document Type: Article

References (32)

1. Alcaide E., and Garay E. R-plasmid transfer in Salmonella spp. isolated from wastewater and sewage-contaminated surface waters. Appl Environ Microbiol 48 (1984) 435-438
2. Bahl R., Sinha A., Poulos C., Whittington D., Sazawal S., Kumar R., et al. Costs of illness due to typhoid fever in an Indian urban slum community: implications for vaccination policy. J Health Popul Nutr 22 (2004) 304-310
3. Brooks W.A., Hossain A., Goswami D., Nahar K., Alam K., and Ahmed N. Bacteremic typhoid fever in children in an urban slum, Bangladesh. Emerg Infect Dis 11 (2005) 326-329
4. Crump J.A., Luby S.P., and Mintz E.D. The global burden of typhoid fever. Bull World Health Organ 82 (2004) 346-353
5. Cobbold R.N., Rice D.H., Davis M.A., Besser T.E., and Hancock D.D. Long-term persistence of multi-drug-resistant Salmonella enterica serovar Newport in two dairy herds. J Amer Vet Med Assoc 228 (2006) 585-591
6. CPCB. Annual Report 2001-2002. Chapter V: Air and Water Quality Monitoring Network. http://www.cpcb.nic.in/oldwebsite/ar2002/ar1-2ch5.htm [Online] (2002).
7. Daum L.T., Barnes W.J., McAvin J.C., Neidert M.S., Cooper L.A., Huff W.B., et al. Real-time PCR detection of Salmonella in suspect foods from a gastroenteritis outbreak in Kerr County, Texas. J Clin Microbiol 40 (2002) 3050-3052
8. Ferguson C., Husman A.M.R., Altavilla N., Deere D., and Ashbolt N. Fate and transport of surface water pathogens in watersheds. Crit Rev Environ Sci Tech 33 (2003) 299-361
9. Galan J.E. Molecular bases of Salmonella entry into host cells. Mol Microbiol 20 (1996) 263-271
10. Hadjinicolaou AV, Demetriou VL, Emmanuel MA, Kakoyiannis CK, Kostrikis LG. Molecular beacon-based real-time PCR detection of primary isolates of Salmonella Typhimurium and Salmonella Enteritidis in environmental and clinical samples. BMC Microbiology 2009; doi:10.1186/1471-2180-9-97.
11. Hu C.J., and Gibbs R.A. A comparison of culture methods for the detection of Salmonella in wastewater sludge. Wat Sci Technol 31 (1995) 303-306
12. Jiménez L., Muñiz I., Toranzos G.A., and Hazen T.C. Survival and activity of Salmonella typhimurium and Escherichia coli in tropical freshwater. J Appl Bacteriol 67 (1989) 61-69
13. Jenkins M.B., Endale D.M., and Fisher D.S. Most probable number methodology for quantifying dilute concentrations and fluxes of Salmonella in surface waters. J Appl Microbiol 104 (2008) 1562-1568
14. Josefsen M.H., Krause M., Hansen F., and Hoorfar J. Optimization of a 12-Hour TaqMan PCR-based method for detection of Salmonella bacteria in meat. Appl Environ Microbiol 73 (2007) 3040-3048
15. Khan I.U.H., Gannon V., Kent R., Koning W., Lapen D.R., Miller J., et al. Development of a rapid quantitative PCR assay for direct detection and quantification of culturable and non-culturable Escherichia coli from agricultural watersheds. J Appl Microbiol Meth 69 (2007) 480-488
16. Lehloesa L.J., and Muyima N.Y.O. Evaluation of the impact of household treatment procedures on the quality of groundwater supplies in the rural community of the Victoria district, Eastern Cape. Water SA 26 (2000) 285-290
17. Malorny B., Hoorfar J., Bunge C., and Helmuth R. Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard. Appl Environ Microbiol 69 (2003) 290-296
18. Malorny B., Bunge C., and Helmuth R. A real-time PCR for the detection of Salmonella Enteritidis in poultry meat and consumption eggs. J Microbiol Meth 70 (2007) 245-251
19. Malorny B., Löfström C., Wagner M., Krämer N., and Hoorfar J. Enumeration of Salmonella bacteria in food and feed samples by real-time PCR for quantitative microbial risk assessment. Appl Environ Microbiol 74 (2008) 1299-1304
20. McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., et al. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature 413 (2001) 852-856
21. Moganedi K.L.M., Goyvaerts E.M.A., Venter S.N., and Sibara M.M. Optimisation of the PCR-invA primers for the detection of Salmonella in drinking and surface waters following a pre-cultivation step. Water SA 33 (2007) 195-202
22. Ochiai R.L., Acosta C.J., Danovaro -Holliday M.C., Baiqing D., Bhattacharya S.K., Agtini M.D., et al. Domi Typhoid Study Group. A study of typhoid fever in five Asian countries: disease burden and implications for controls. Bull World Health Organ 86 (2008) 260-268
23. Qadri F., Svennerholm A.M., Faruque A.S.G., and Sack R.B. Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment and prevention. Clin Microbiol Rev 18 (2005) 465-483
24. Rahn K., De Grandis S.A., Clarke R.C., McEwen S.A., Gala'n J.E., Ginocchio C., et al. Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol Cell Probes 6 (1992) 271-279
25. Ram S., Vajpayee P., and Shanker R. Prevalence of multi-antimicrobial-agent resistant, Shiga toxin and enterotoxin producing Escherichia coli in surface waters of River Ganga. Environ Sci Technol 41 (2007) 7383-7388
26. Ram S., Vajpayee P., and Shanker R. Rapid culture-independent quantitative detection of enterotoxigenic Escherichia coli in surface waters by real-time PCR with molecular beacon. Environ Sci Technol 42 (2008) 4577-4582
27. Ram S., Vajpayee P., and Shanker R. Contamination of potable water distribution systems by multiantimicrobial -resistant enterohemorrhagic Escherichia coli. Environ Helth Perspect 116 (2008) 448-452
28. Ram S., Vajpayee P., Tripathi U., Singh R.L., Seth P.K., and Shanker R. Determination of antimicrobial resistance and virulence gene signatures in surface water isolates of Escherichia coli. J Appl Microbiol 105 (2008) 1899-1908
29. Ram S., Vajpayee P., Singh R.L., and Shanker R. Surface water of a perennial river exhibits multi-antimicrobial resistant shiga toxin and enterotoxin producing Escherichia coli Ecotoxicol Environ Saf 72 (2009) 490-495
30. Schefler W.C. Statistics for Biological Sciences (1969), Addison-Wesley Publishing Company, MenloPark, CA
31. Seo K.H., Valentin-Bon I.E., and Brackett R.E. Detection and enumeration of Salmonella enteritidis in homemade ice cream associated with an outbreak: comparison of conventional and real-time PCR methods. J Food Prot 69 (2006) 639-643
32. Voetsch A.C., Gilder T.J.V., Angulo F.J., Farley M.M., Shallow S., Marcus R., et al. Food Net estimate of the burden of illness caused by non-typhoidal Salmonella infections in the United States. Clin Infect Dis 38 (2004) S127-S134