Selective quantification of viable Escherichia coli bacteria in biosolids by quantitative PCR with propidium monoazide modification

Applied and Environmental Microbiology

Volumn 77, Issue 13, 2011, Pages 4329-4335

  Taskin, Bilgin ^a,b   Gozen, Ayse Gul ^a   Duran, Metin ^b  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

^b Villanova University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ABSOLUTE QUANTIFICATION; ALTERNATIVE APPROACH; AZIDE GROUP; DEAD CELLS; DNA AMPLIFICATION; ENVIRONMENTAL SAMPLE; ESCHERICHIA COLI BACTERIA; ETHIDIUM MONOAZIDE; INDICATOR ORGANISMS; INTERCALATING DYE; LIVE CELL; MEMBRANE INTEGRITY; MODEL ORGANISMS; PCR AMPLIFICATION; PROPIDIUM; PUBLIC HEALTH RISKS; QUANTITATIVE PCR; REAL-TIME PCR; TARGET SEQUENCES; TOTAL SUSPENDED SOLIDS; VIABLE CELLS;

BIOLOGICAL MATERIALS; BIOSOLIDS; CELL DEATH; DNA; ESCHERICHIA COLI; EXPERIMENTS; GENES; HEALTH RISKS;

POLYMERASE CHAIN REACTION;

AZIDE; DRUG DERIVATIVE; ENZYME INHIBITOR; PROPIDIUM IODIDE; PROPIDIUM MONOAZIDE;

APOPTOSIS; BIOSOLID; COLIFORM BACTERIUM; DYE; FLUORESCENCE; GENE EXPRESSION; GENETIC ANALYSIS; HEALTH RISK; ORGANIC COMPOUND; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; SUSPENDED LOAD;

ARTICLE; BACTERIAL LOAD; ESCHERICHIA COLI; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIAL VIABILITY; MICROBIOLOGY; PHYSIOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

AZIDES; BACTERIAL LOAD; ENVIRONMENTAL MICROBIOLOGY; ENZYME INHIBITORS; ESCHERICHIA COLI; MICROBIAL VIABILITY; POLYMERASE CHAIN REACTION; PROPIDIUM; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 79960080969     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02895-10     Document Type: Article

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