LGP2 Plays a Critical Role in Sensitizing mda-5 to Activation by Double-Stranded RNA

PLoS ONE

Volumn 8, Issue 5, 2013, Pages

  Childs, Kay S ^a   Randall, Richard E ^b   Goodbourn, Stephen ^a  

^a ST GEORGE'S UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY OF ST ANDREWS   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BETA INTERFERON; DEAD BOX PROTEIN; DOUBLE STRANDED RNA; HELICASE; LABORATORY OF GENETICS AND PHYSIOLOGY 2 PROTEIN; MELANOMA DIFFERENTIATION ASSOCIATED GENE 5 PROTEIN; POLYINOSINIC POLYCYTIDYLIC ACID; RETINOIC ACID INDUCIBLE PROTEIN I; UNCLASSIFIED DRUG; VIRUS PROTEIN V;

ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTERFERON INDUCTION; PARAMYXOVIRUS; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION;

BINDING SITES; DEAD-BOX RNA HELICASES; HEK293 CELLS; HUMANS; IMMUNOBLOTTING; INTERFERON TYPE I; POLY I-C; PROTEIN BINDING; RNA HELICASES; RNA INTERFERENCE; RNA, DOUBLE-STRANDED; TRANSCRIPTIONAL ACTIVATION; TWO-HYBRID SYSTEM TECHNIQUES; VIRAL PROTEINS;

EID: 84877326407     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0064202     Document Type: Article

References (39)

1. Kumagai Y, Akira S, (2010) Identification and functions of pattern-recognition receptors. J Allergy Clin Immunol. 125: 985-992.
2. Hornung V, Ellegast J, Kim S, Brzozka K, Jung A, et al. (2006) 5′-Triphosphate RNA is the ligand for RIG-I. Science 314: 994-997.
3. Pichlmair A, Schulz O, Tan CP, Naslund TI, Liljestrom P, et al. (2006) RIG-I-mediated antiviral responses to single-stranded RNA bearing 5′-phosphates. Science 314: 997-1001.
4. Schlee M, Roth A, Hornung V, Hagmann CA, Wimmenauer V, et al. (2009) Recognition of 5′ triphosphate by RIG-I helicase requires short blunt double-stranded RNA as contained in panhandle of negative-strand virus. Immunity 3: 25-34.
5. Kato H, Takeuchi O, Mikamo-Satoh E, Hirai R, Kawai T, et al. (2008) Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid-inducible gene-I and melanoma differentiation-associated gene 5. J Exp Med 205: 1601-1610.
6. Pichlmair A, Schulz O, Tan CP, Rehwinkel J, Kato H, et al. (2009) Activation of MDA5 requires higher-order RNA structures generated during virus infection. J Virol 83: 10761-10769.
7. Leung DW, Amarasinghe GK, (2012) Structural insights into RNA recognition and activation of RIG-I-like receptors. Curr Opin Struct Biol 22: 297-303.
8. Binder M, Eberle F, Seitz S, Mucke N, Huber CM, et al. (2011) Molecular mechanism of signal perception and integration by the innate immune sensor retinoic acid-inducible gene-I (RIG-I). J Biol Chem 286: 27278-27287.
9. Peisley A, Lin C, Wu B, Orme-Johnson M, Liu M, et al. (2011) Cooperative assembly and dynamic disassembly of MDA5 filaments for viral dsRNA recognition. Proc Natl Acad Sci U S A 108: 21010-21015.
10. Berke IC, Yu X, Modis Y, Egelman EH, (2012) MDA5 assembles into a polar helical filament on dsRNA. Proc Natl Acad Sci U S A 109: 18437-18441.
11. Goodbourn S, Randall RE, (2009) The regulation of type I interferon production by paramyxoviruses. J Interf Cyto Res 29: 539-547.
12. Levy DE, Marié IJ, Durbin JE, (2011) Induction and function of type I and III interferon in response to viral infection. Curr Opin Virol 1: 476-486.
13. Rothenfusser S, Goutagny N, DiPerna G, Gong M, Monks BG, et al. (2005) The RNA helicase Lgp2 inhibits TLR-independent sensing of viral replication by retinoic acid-inducible gene-I. J Immunol 175: 5260-5268.
14. Yoneyama M, Kikuchi M, Matsumoto K, Imaizumi T, Miyagishi M, et al. (2005) Shared and unique functions of the DExD/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity. J Immunol 175: 2851-2858.
15. Randall R, Goodbourn S, (2008) Interferons and viruses: an interplay between induction, signalling, antiviral responses and virus countermeasures. J Gen Virol 89: 1-47.
16. Johnston MD, (1981) The characteristics required for a Sendai virus preparation to induce high levels of interferon in human lymphoblastoid cells. J Gen Virol 56: 175-184.
17. Strahle L, Garcin D, Kolakofsky D, (2006) Sendai virus defective-interfering genomes and the activation of interferon-beta. Virology 20: 101-111.
18. Killip MJ, Young DF, Ross CS, Chen S, Goodbourn S, et al. (2011) Failure to activate the IFN-β promoter by a paramyxovirus lacking an interferon antagonist. Virology 415: 39-46.
19. Killip MJ, Young D, Gatherer D, Ross C, Short J, et al. (2013) Deep sequencing analysis of defective genomes of parainfluenza virus 5 and their role in interferon induction. J Virol in press.
20. Kato H, Takeuchi O, Sato S, Yoneyama M, Yamamoto M, et al. (2006) Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature 441: 101-105.
21. Loo YM, Gale M Jr, (2011) Immune signaling by RIG-I-like receptors. Immunity 34: 680-692.
22. Komuro A, Horvath CM, (2006) RNA- and virus-independent inhibition of antiviral signaling by RNA helicase LGP2. J Virol 80: 12332-12342.
23. Venkataraman T, Valdes M, Elsby R, Kakuta S, Caceres G, et al. (2007) Loss of DExD/H box RNA helicase LGP2 manifests disparate antiviral responses. J Immunol 178: 6444-6455.
24. Satoh T, Kato H, Kumagai Y, Yoneyama M, Sato S, et al. (2010) LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses. Proc Natl Acad Sci U S A 107: 1512-1517.
25. Loo YM, Fornek J, Crochet N, Bajwa G, Perwitasari O, et al. (2008) Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity. J Virol 82: 335-345.
26. King P, Goodbourn S, (1994) The beta-interferon promoter responds to priming through multiple independent regulatory elements. J Biol Chem 269: 30609-30615.
27. Masson N, Ellis M, Goodbourn S, Lee KA, (1992) Cyclic AMP response element-binding protein and the catalytic subunit of protein kinase A are present in F9 embryonal carcinoma cells but are unable to activate the somatostatin promoter. Mol Cell Biol 12: 1096-1106.
28. Marais R, Light Y, Paterson HF, Marshall CJ, (1995) Ras recruits Raf-1 to the plasma membrane for activation by tyrosine phosphorylation. EMBO J 14: 3136-3145.
29. Childs K, Stock N, Ross C, Andrejeva J, Hilton L, et al. (2007) mda-5, but not RIG-I, is a common target for paramyxovirus V proteins. Virology 359: 190-200.
30. Childs K, Randall R, Goodbourn S, (2012) Paramyxovirus V proteins interact with the RNA Helicase LGP2 to inhibit RIG-I-dependent interferon induction. J Virol 86: 3411-3421.
31. Childs KS, Andrejeva J, Randall RE, Goodbourn S, (2009) Mechanism of mda-5 Inhibition by paramyxovirus V proteins. J Virol 83: 1465-1473.
32. Didcock L, Young DF, Goodbourn S, Randall RE, (1999) The V protein of simian virus 5 inhibits interferon signalling by targeting STAT1 for proteasome-mediated degradation. J Virol 73: 9928-9933.
33. Lennon G, Auffray C, Polymeropoulos M, Soares MB, (1996) The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. Genomics 33: 151-152.
34. Pippig DA, Hellmuth JC, Cui S, Kirchhofer A, Lammens K, et al. (2009) The regulatory domain of the RIG-I family ATPase LGP2 senses double-stranded RNA. Nucl Acids Res 37: 2014-2025.
35. Bamming D, Horvath CM, (2009) Regulation of signal transduction by enzymatically inactive antiviral RNA helicase proteins MDA5, RIG-I, and LGP2. J Biol Chem 284: 9700-9712.
36. Li X, Ranjith-Kumar CT, Brooks MT, Dharmaiah S, Herr AB, et al. (2009) The RIG-I-like receptor LGP2 recognizes the termini of double-stranded RNA. J Biol Chem 284: 13881-13891.
37. Andrejeva J, Childs KS, Young DF, Carlos TS, Stock N, et al. (2004) The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter. Proc Natl Acad Sci U S A 101: 17264-17269.
38. Berke IC, Modis Y, (2012) MDA5 cooperatively forms dimers and ATP-sensitive filaments upon binding double-stranded RNA. EMBO J 31: 1714-1726.
39. Bruns AM, Pollpeter D, Hadizadeh N, Myong S, Marko JF, et al. (2013) ATP hydrolysis enhances RNA recognition and antiviral signal transduction by the innate immune sensor, Laboratory of Genetics and Physiology 2 (LGP2). J Biol Chem 288: 938-946.