Gene and genome construction in yeast

Current Protocols in Molecular Biology

Volumn , Issue SUPPL.94, 2011, Pages

  Gibson, Daniel G ^a  

^a J CRAIG VENTER INSTITUTE   (United States)

Author keywords

DNA assembly; DNA construction; Gene construction; Genome construction; In vivo recombination; Synthetic biology; Yeast recombination; Yeast transformation

Indexed keywords

AMPICILLIN; BUFFER; CARBENICILLIN; DNA FRAGMENT; DOUBLE STRANDED DNA; ENZYME; NOTI RESTRICTION ENZYME; OLIGONUCLEOTIDE; PLASMID DNA; PRIMER DNA; RESTRICTION ENDONUCLEASE; SINGLE STRANDED DNA; UNCLASSIFIED DRUG; ZYMOLYASE;

AGAR GEL ELECTROPHORESIS; ARTICLE; AT RICH SEQUENCE; BACTERIUM TRANSFORMATION; CELL REGENERATION; CELL SUSPENSION; CENTRIFUGATION; CLONING VECTOR; DNA REPLICATION; DNA SYNTHESIS; DNA TRANSFER; ESCHERICHIA COLI; FREEZING; GENE CONSTRUCT; GENOMIC INSTABILITY; INCUBATION TEMPERATURE; INOCULATION; LOW MELTING POINT AGAROSE GEL ELECTROPHORESIS; MELTING POINT; MICROCENTRIFUGATION; MULTIPLEX POLYMERASE CHAIN REACTION; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRECIPITATION; PRIORITY JOURNAL; ROOM TEMPERATURE; SHUTTLE VECTOR; SPHEROPLAST; THAWING; YEAST; YEAST CELL;

BACTERIA (MICROORGANISMS); SACCHAROMYCES CEREVISIAE;

EID: 84857658214     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb0322s94     Document Type: Article

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