Design and validation of a homogeneous time-resolved fluorescence-based leptin receptor binding assay

Analytical Biochemistry

Volumn 436, Issue 1, 2013, Pages 1-9

  Vauthier, Virginie ^a,b,c   Derviaux, Carine ^a,b,c   Douayry, Najim ^d   Roux, Thomas ^d   Trinquet, Eric ^d   Jockers, Ralf ^a,b,c   Dam, Julie ^a,b,c  

^a INSERM   (France)

^b CNRS   (France)

^c UNIVERSITÉ PARIS DESCARTES   (France)

^d Cisbio Bioassays   (France)

Author keywords

Binding; High throughput screening; HTRF; Leptin antagonist; Leptin receptor

Indexed keywords

DIAGNOSIS; DISSOCIATION; ENERGY TRANSFER; FLUORESCENCE; HORMONES; LIGANDS; SIGNAL TO NOISE RATIO;

BINDING; HIGH THROUGHPUT SCREENING; HTRF; LEPTIN ANTAGONIST; LEPTIN RECEPTORS;

BINDING ENERGY;

FLUORESCENT DYE; LEPTIN RECEPTOR;

ARTICLE; BINDING ASSAY; DISSOCIATION CONSTANT; ENERGY TRANSFER; FLUORESCENCE ANALYSIS; PRIORITY JOURNAL; PROCESS DESIGN; RECEPTOR BINDING; REPRODUCIBILITY; SIGNAL NOISE RATIO; VALIDATION PROCESS;

ALKALINE PHOSPHATASE; BINDING SITES; CELLS, CULTURED; FLUORESCENCE; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HORMONE ANTAGONISTS; HUMANS; LEPTIN; LIGANDS; PROTEIN BINDING; RECEPTORS, LEPTIN; REPRODUCIBILITY OF RESULTS; TIME FACTORS;

EID: 84875196370     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.12.013     Document Type: Article

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