Identification of the hydrophobia strand in the A-B loop of leptin as major binding site III: Implications for large-scale preparation of potent recombinant human and ovine leptin antagonists

Biochemical Journal

Volumn 391, Issue 2, 2005, Pages 221-230

  Niv Spector, Leonora ^a   Gonen Berger, Dana ^a   Gourdou, Isabelle ^b   Biener, Eva ^a   Gussakovsky, Eugene E ^c,d   Benomar, Yackir ^e   Ramanujan, Krishnan V ^f   Taouis, Mohammed ^e   Herman, Brian ^f   Callebaut, Isabelle ^g   Djiane, Jean ^b   Gertler, Arieh ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b CEMAGREF   (France)

^c BAR ILAN UNIVERSITY   (Israel)

^d VOLCANI CENTER   (Israel)

^e UNIVERSITÉ PARIS SACLAY   (France)

^f Mays Cancer Center at UT Health San Antonio   (United States)

^g UNIVERSITÉ PIERRE ET MARIE CURIE   (France)

Author keywords

A B loop of leptin; Antagonists; Fluorescence resonance energy transfer (FRET); Hydrophobic strand; Immunoglobulin like domain (IGD); Site directed mutagenesis

Indexed keywords

AMINO ACIDS; DIMERIZATION; ENERGY TRANSFER; FILTRATION; FLUORESCENCE; GELS; GENETIC ENGINEERING; HYDROPHOBICITY; MUTAGENESIS; PROTEINS; RESONANCE;

A-B LOOP OF LEPTIN; ANTAGONISTS; FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET); HYDROPHOBIC STRANDS; IMMUNOGLOBULIN-LIKE DOMAIN (IGD); SITE-DIRECTED MUTAGENESIS;

BIOCHEMISTRY;

AMINO ACID; IMMUNOGLOBULIN; LEPTIN RECEPTOR; RECOMBINANT LEPTIN ANTAGONIST; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; CELL SURFACE RECEPTOR; LEPTIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BETA SHEET; BINDING SITE; CLUSTER ANALYSIS; CONTROLLED STUDY; DRUG RECEPTOR BINDING; FLUORESCENCE RESONANCE ENERGY TRANSFER; GEL FILTRATION; HUMAN; HUMAN CELL; HYDROPHOBICITY; LARGE SCALE PRODUCTION; OLIGOMERIZATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PREDICTION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; RECEPTOR BINDING; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; ANIMAL; CHEMISTRY; DRUG ANTAGONISM; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; MOUSE; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; SHEEP; TUMOR CELL LINE;

OVIS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CELL LINE, TUMOR; GENE EXPRESSION REGULATION; HUMANS; HYDROPHOBICITY; LEPTIN; MICE; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; RECEPTORS, CELL SURFACE; RECOMBINANT PROTEINS; SHEEP;

EID: 25144489423     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20050457     Document Type: Article

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