Flow cytometric 96-well microplate-based in vitro micronucleus assay with human TK6 cells: Protocol optimization and transferability assessment

Environmental and Molecular Mutagenesis

Volumn 54, Issue 3, 2013, Pages 180-194

  Bryce, Steven M ^a   Avlasevich, Svetlana L ^a   Bemis, Jeffrey C ^a   Tate, Matthew ^b   Walmsley, Richard M ^b   Saad, Frédéric ^c   Van Dijck, Kris ^c   De Boeck, Marlies ^c   Van Goethem, Freddy ^c   Lukamowicz Rajska, Magdalena ^d   Elhajouji, Azeddine ^d   Dertinger, Stephen D ^a  

^a LITRON LABORATORIES   (United States)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^d NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Automation; Flow cytometry; Genotoxicity; Hazard assessment; Micronuclei; Safety assessment; Screening; TK6 cells

Indexed keywords

AMPICILLIN; ANTHRANILIC ACID; APHIDICOLIN; BLEOMYCIN; CADMIUM CHLORIDE; CAMPTOTHECIN; CARBAMIC ACID METHYL ESTER; CARBENDAZIM; CISPLATIN; COLCHICINE; CYTARABINE; DEXAMETHASONE; DIETHYLSTILBESTROL; ERYTHROMYCIN; ETHIDIUM; ETHIONAMIDE; ETOPOSIDE; FLUOROURACIL; HYDROQUINONE; HYDROXYUREA; MESYLIC ACID METHYL ESTER; METHOTREXATE; NALIDIXIC ACID; PHTHALIC ANHYDRIDE; PYRENE; SODIUM CHLORIDE; SULFAFURAZOLE; TUNICAMYCIN; UNINDEXED DRUG; VINCRISTINE SULFATE;

APOPTOSIS; ARTICLE; AUTOMATION; CLINICAL PROTOCOL; CONTROLLED STUDY; CYTOTOXICITY; FLOW CYTOMETRY; GENOTOXICITY; HUMAN; HUMAN CELL; IN VITRO STUDY; MICRONUCLEUS; PROCESS OPTIMIZATION; REPRODUCIBILITY;

APOPTOSIS; CELL COUNT; CELL LINE; FLOW CYTOMETRY; HUMANS; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; MUTAGENS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84875050640     PISSN: 08936692     EISSN: 10982280     Source Type: Journal    
DOI: 10.1002/em.21760     Document Type: Article

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