Interlaboratory evaluation of a flow cytometric, high content in vitro micronucleus assay

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 650, Issue 2, 2008, Pages 181-195

  Bryce, Steven M ^a   Avlasevich, Svetlana L ^a   Bemis, Jeffrey C ^a   Lukamowicz, Magdalena ^b   Elhajouji, Azeddine ^b   Van Goethem, Freddy ^c   De Boeck, Marlies ^c   Beerens, Dominiek ^c   Aerts, Hilde ^c   Van Gompel, Jacky ^c   Collins, Joanne E ^d   Ellis, Patricia C ^d   White, Angela T ^d   Lynch, Anthony M ^d   Dertinger, Stephen D ^a  

^a LITRON LABORATORIES   (United States)

^b NOVARTIS PHARMA AG   (Switzerland)

^c JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^d GLAXOSMITHKLINE   (United Kingdom)

Author keywords

Automation; Cytotoxicity; Flow cytometry; Genotoxicity; L5178Y; Micronuclei

Indexed keywords

DEXAMETHASONE; ETOPOSIDE; MITOMYCIN C; STAUROSPORINE; SUCROSE; VINBLASTINE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; FLOW CYTOMETRY; GENOTOXICITY; IN VITRO STUDY; MICRONUCLEUS; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; APOPTOSIS; AUTOMATION; CELL LINE, TUMOR; DEXAMETHASONE; FLOW CYTOMETRY; MICE; MICRONUCLEUS TESTS; MITOMYCIN; STAUROSPORINE; SUCROSE; VINBLASTINE;

EID: 39149095273     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2007.11.006     Document Type: Article

References (30)

1. Kirsch-Volders M., Sofuni T., Aardema M., Albertini S., Eastmond D., Fenech M., Ishidate Jr. M., Kirchner S., Lorge E., Morita T., Norppa H., Surrallés J., Vanhauwaert A., and Wakata A. Report from the in vitro micronucleus assay working group. Mutat. Res. 540 (2003) 153-163
2. Matsushima T., Hayashi M., Matsuoka A., Ishidate Jr. M., Miura K.F., Shimizu H., Morimoto K., Ogura H., Mure K., Koshi K., and Sufuni T. Validation study of the in vitro micronucleus test in a Chinese hamster lung cell line (CHL/IU). Mutagenesis 14 (1999) 569-580
3. Miller B., Potter-Locher F., Seelbach A., Stopper H., Utesch D., and Madle S. Evaluation of the in vitro micronucleus test as an alternative to the in vitro chromosome aberration assay: position of the GUM working group on the in vitro micronucleus test. Mutat. Res. 410 (1998) 81-116
4. Lorge E., Thybaud V., Aardema M.J., Oliver J., Wakata A., Lorenzon G., and Marzin D. SFTG international collaborative study on in vitro micronucleus test. I: General conditions and overall conclusions of the study. Mutat. Res. 607 (2006) 13-36
5. Lorge E., Lambert C., Gervais V., Becourt-Lhote N., Delongeas J.-L., and Claude N. Genetic toxicity assessment: employing the best science for human safety evaluation. Part II: Performances of the in vitro micronucleus test compared to the mouse lymphoma assay and the in vitro chromosome aberration assay. Toxicol. Sci. 96 (2007) 214-217
6. Organisation for Economic Co-operation and Development (OECD), Guidelines for the Testing of Chemicals, Draft Guideline 487: In Vitro Micronucleus Test, December 21, 2006 (2nd version).
7. Nüsse M., and Kramer J. Flow cytometric analysis of micronuclei found in cells after irradiation. Cytometry 5 (1984) 20-25
8. Schreiber G.A., Beisker W., Bauchinger M., and Nüsse M. Multiparametric flow cytometric analysis of radiation-induced micronuclei in mammalian cell cultures. Cytometry 13 (1992) 90-102
9. Wessels J., and Nüsse M. Flow cytometric detection of micronuclei by combined staining of DNA and membranes. Cytometry 19 (1995) 201-208
10. 2+ subpopulation of human lymphocytes enriched by magnetic separation. Int. J. Radiat. Biol. 67 (1995) 193-202
11. Nüsse M., and Marx K. Flow cytometric analysis of micronuclei in cell cultures and human lymphocytes: advantages and disadvantages. Mutat. Res. 392 (1997) 109-115
12. Avlasevich S.L., Bryce S.M., Cairns S.E., and Dertinger S.D. In vitro micronucleus scoring by flow cytometry: differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliability. Environ. Mol. Mutagen. 47 (2006) 56-66
13. Bryce S.M., Bemis J.C., Avlasevich S.L., and Dertinger S.D. In vitro micronucleus assay scored by flow cytometry provides a comprehensive evaluation of cytogenetic damage and cytotoxicity. Mutat. Res. 630 (2007) 78-91
14. Greenwood S.K., Hill R.B., Sun J.T., Armstrong M.J., Johnson T.E., Gara J.P., and Galloway S.M. Population doubling: a simple and more accurate estimation of cell growth suppression in the in vitro assay for chromosomal aberrations that reduces irrelevant positive results. Environ. Mol. Mutagen. 43 (2004) 36-44
15. Fenech M., Chang W.P., Kirsch-Volders M., Holland N., Bonassi S., and Zeiger E. HUMN project: detailed description of the scoring criteria for the cytokinesis block micronucleus assay using isolated human lymphocyte cultures. Mutat. Res. 534 (2003) 65-75
16. Meintieres S., Biola A., Pallardy M., and Marzin D. Apoptosis can be a confusing factor in in vitro clastogenic assays. Mutagenesis 16 (2001) 243-250
17. Meintieres S., Biola A., Pallardy M., and Marzin D. Using CTLL-2 and CTLL-2 bcl2 cells to avoid interference by apoptosis in the in vitro micronucleus test. Environ. Mol. Mutagen. 41 (2003) 14-27
18. Kirkland D., Aardema M., Henderson L., and Müller L. Evaluation of the ability of a battery of three in vitro genotoxicity tests to discriminate rodent carcinogens and non-carcinogens. I: Sensitivity, specificity and relative predictivity. Mutat. Res. 584 (2005) 1-256
19. Kirkland D., Pfuhler S., Tweats D., Aardema M., Corvi R., Darroudi F., Elhajouji A., Glatt H., Hastwell P., Hayashi M., Kasper P., Kirchner S., Lynch A., Marzin D., Maurici D., Meunier J.-R., Müller L., Nohynek G., Parry J., Parry E., Thybaud V., Tice R., van Benthem J., Vanparys P., and White P. How to reduce false positive results when undertaking in vitro genotoxicity testing and thus avoid unnecessary follow-up animal tests: report of an ECVAM Workshop. Mutat. Res. 628 (2007) 31-55
20. Hilliard C.A., Armstrong M.J., Bradt C.I., Hill R.B., Greenwood S.K., and Galloway S.M. Chromosome aberrations in vitro related to cytotoxicity of nonmutagenic chemicals and metabolic poisons. Environ. Mol. Mutagen. 31 (1998) 316-326
21. Galloway S.M. Cytotoxicity and chromosome aberrations in vitro: experience in industry and the case for an upper limit on toxicity in the aberration assay. Environ. Mol. Mutagen. 35 (2000) 191-201
22. Fellows M.D., and O'Donovan M.R. Cytotoxicity in cultured mammalian cells is a function of the method used to estimate it. Mutagenesis 22 (2007) 275-280
23. ® Kit for the flow cytometric enumeration of micronuclei (MN) in mammalian cells, manuscript in preparation.
24. Matsuoka A., Yamazaki N., Suzuki T., Hayashi M., and Sofuni T. Evaluation of the micronucleus test using a Chinese hamster cell line as an alternative to the conventional in vitro chromosomal aberration test. Mutat. Res. 272 (1993) 223-236
25. Garriott M.L., Phelps J.B., and Hoffman W.P. A protocol for the in vitro micronucleus test. I: Contributions to the development of a protocol suitable for regulatory submissions from an examination of 16 chemicals with different mechanisms of action and different levels of activity. Mutat. Res. 517 (2002) 123-134
26. Phelps J.B., Garriott M.L., and Hoffman W.P. A protocol for the in vitro micronucleus test. II: Contributions to the validation of a protocol suitable for regulatory submissions from an examination of 10 chemicals with different mechanisms of action and different levels of activity. Mutat. Res. 521 (2002) 103-112
27. von der Hude W., Kalweit S., Engelhardt G., McKiernan S., Kasper P., Slacik-Erben R., Miltenburger H.G., Honarvar N., Fahrig R., Gorlitz B., Albertini S., Kirchner S., Utesch D., Potter-Locher F., Stopper H., and Madle S. In vitro micronucleus assay with Chinese hamster V79 cells-results of a collaborative study with in situ exposure to 26 chemical substances. Mutat. Res. 468 (2000) 137-163
28. Knasmüller S., Mersch-Sundermann V., Kevekordes S., Darroudi F., Huber W.W., Hoelzl C., Bichler J., and Majer B.J. Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants: current state of knowledge. Toxicology 198 (2004) 315-328
29. Zhan L., Sakamoto H., Sakurab M., Wu S., Zhang L.S., Suzuki T., Hayashi M., and Honma M. Genotoxicity of microcystin-LR in human lymphoblastoid TK6 cells. Mutat. Res. 557 (2004) 1-6
30. Hastwell P.W., Chai L.-L., Roberts K.J., Webster T.W., Harvey J.S., Rees R.W., and Walmsley R.M. High-specificity and high-sensitivity genotoxicity assessment in a human cell line: Validation of the GreenScreen HC GADD45a-GFP genotoxicity assay. Mutat. Res. 607 (2006) 160-175