Effector kinase coupling enables high-throughput screens for direct HIV-1 Nef antagonists with antiretroviral activity

Chemistry and Biology

Volumn 20, Issue 1, 2013, Pages 82-91

  Emert Sedlak, Lori A ^a   Narute, Purushottam ^a   Shu, Sherry T ^a   Poe, Jerrod A ^a   Shi, Haibin ^a   Yanamala, Naveena ^a   Alvarado, John Jeff ^a   Lazo, John S ^a,b   Yeh, Joanne I ^a   Johnston, Paul A ^a,c   Smithgall, Thomas E ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF VIRGINIA   (United States)

^c UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HEMATOPOIETIC CELL KINASE; NEF PROTEIN;

ARTICLE; CONTROLLED STUDY; DIMERIZATION; HIGH THROUGHPUT SCREENING; HIGHLY ACTIVE ANTIRETROVIRAL THERAPY; HOST CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR DOCKING; PRIORITY JOURNAL; VIRUS REPLICATION;

ANTI-RETROVIRAL AGENTS; CELL LINE; HIV INFECTIONS; HIV-1; HUMANS; MOLECULAR DOCKING SIMULATION; NEF GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; PROTEIN MULTIMERIZATION; PROTO-ONCOGENE PROTEINS C-HCK; PYRAZOLES; SMALL MOLECULE LIBRARIES; VIRUS REPLICATION;

EID: 84872898071     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chembiol.2012.11.005     Document Type: Article

References (40)

1. C. Aiken, and D. Trono Nef stimulates human immunodeficiency virus type 1 proviral DNA synthesis J. Virol. 69 1995 5048 5056
2. S.T. Arold, and A.S. Baur Dynamic Nef and Nef dynamics: how structure could explain the complex activities of this small HIV protein Trends Biochem. Sci. 26 2001 356 363 (Pubitemid 32530653)
3. K.M. Atkins, L. Thomas, R.T. Youker, M.J. Harriff, F. Pissani, H. You, and G. Thomas HIV-1 Nef binds PACS-2 to assemble a multikinase cascade that triggers major histocompatibility complex class I (MHC-I) down-regulation: analysis using short interfering RNA and knock-out mice J. Biol. Chem. 283 2008 11772 11784
4. S. Betzi, A. Restouin, S. Opi, S.T. Arold, I. Parrot, F. Guerlesquin, X. Morelli, and Y. Collette Protein protein interaction inhibition (2P2I) combining high throughput and virtual screening: application to the HIV-1 Nef protein Proc. Natl. Acad. Sci. USA 104 2007 19256 19261
5. M.Y. Chowers, C.A. Spina, T.J. Kwoh, N.J. Fitch, D.D. Richman, and J.C. Guatelli Optimal infectivity in vitro of human immunodeficiency virus type 1 requires an intact nef gene J. Virol. 68 1994 2906 2914 (Pubitemid 24129738)
6. N. Chutiwitoonchai, M. Hiyoshi, P. Mwimanzi, T. Ueno, A. Adachi, H. Ode, H. Sato, O.T. Fackler, S. Okada, and S. Suzu The identification of a small molecule compound that reduces HIV-1 Nef-mediated viral infectivity enhancement PLoS One 6 2011 e27696
7. N.J. Deacon, A. Tsykin, A. Solomon, K. Smith, M. Ludford-Menting, D.J. Hooker, D.A. McPhee, A.L. Greenway, A. Ellett, and C. Chatfield Genomic structure of an attenuated quasi species of HIV-1 from a blood transfusion donor and recipients Science 270 1995 988 991
8. C.A. Derdeyn, J.M. Decker, J.N. Sfakianos, X. Wu, W.A. O'Brien, L. Ratner, J.C. Kappes, G.M. Shaw, and E. Hunter Sensitivity of human immunodeficiency virus type 1 to the fusion inhibitor T-20 is modulated by coreceptor specificity defined by the V3 loop of gp120 J. Virol. 74 2000 8358 8367 (Pubitemid 30666686)
9. J.D. Dikeakos, K.M. Atkins, L. Thomas, L. Emert-Sedlak, I.J. Byeon, J. Jung, J. Ahn, M.D. Wortman, B. Kukull, and M. Saito Small molecule inhibition of HIV-1-induced MHC-I down-regulation identifies a temporally regulated switch in Nef action Mol. Biol. Cell 21 2010 3279 3292
10. W.B. Dyer, A.F. Geczy, S.J. Kent, L.B. McIntyre, S.A. Blasdall, J.C. Learmont, and J.S. Sullivan Lymphoproliferative immune function in the Sydney Blood Bank Cohort, infected with natural nef/long terminal repeat mutants, and in other long-term survivors of transfusion-acquired HIV-1 infection AIDS 11 1997 1565 1574 (Pubitemid 27460245)
11. L. Emert-Sedlak, T. Kodama, E.C. Lerner, W. Dai, C. Foster, B.W. Day, J.S. Lazo, and T.E. Smithgall Chemical library screens targeting an HIV-1 accessory factor/host cell kinase complex identify novel antiretroviral compounds ACS Chem. Biol. 4 2009 939 947
12. M. Geyer, O.T. Fackler, and B.M. Peterlin Structure - function relationships in HIV-1 Nef EMBO Rep. 2 2001 580 585 (Pubitemid 32707703)
13. R.K. Gupta, A. Hill, A.W. Sawyer, A. Cozzi-Lepri, V. von Wyl, S. Yerly, V.D. Lima, H.F. Günthard, C. Gilks, and D. Pillay Virological monitoring and resistance to first-line highly active antiretroviral therapy in adults infected with HIV-1 treated under WHO guidelines: a systematic review and meta-analysis Lancet Infect. Dis. 9 2009 409 417
14. Z. Hanna, D.G. Kay, N. Rebai, A. Guimond, S. Jothy, and P. Jolicoeur Nef harbors a major determinant of pathogenicity for an AIDS-like disease induced by HIV-1 in transgenic mice Cell 95 1998 163 175 (Pubitemid 28473778)
15. L. Jason-Moller, M. Murphy, and J. Bruno Overview of Biacore systems and their applications Curr. Protoc. Protein Sci. 2006 Chapter 19, Unit 19.13
16. P. Jolicoeur The CD4C/HIV(Nef)transgenic model of AIDS Curr. HIV Res. 9 2011 524 530
17. A.M. Joseph, M. Kumar, and D. Mitra Nef: "necessary and enforcing factor" in HIV infection Curr. HIV Res. 3 2005 87 94 (Pubitemid 40081288)
18. H.W. Kestler 3rd, D.J. Ringler, K. Mori, D.L. Panicali, P.K. Sehgal, M.D. Daniel, and R.C. Desrosiers Importance of the nef gene for maintenance of high virus loads and for development of AIDS Cell 65 1991 651 662
19. W.M. Kim, A.B. Sigalov, and L.J. Stern Pseudo-merohedral twinning and noncrystallographic symmetry in orthorhombic crystals of SIVmac239 Nef core domain bound to different-length TCRzeta fragments Acta Crystallogr. D Biol. Crystallogr. 66 2010 163 175
20. F. Kirchhoff, T.C. Greenough, D.B. Brettler, J.L. Sullivan, and R.C. Desrosiers Brief report: absence of intact nef sequences in a long-term survivor with nonprogressive HIV-1 infection N. Engl. J. Med. 332 1995 228 232
21. K. Kuroki, and K. Maenaka Analysis of receptor-ligand interactions by surface plasmon resonance Methods Mol. Biol. 748 2011 83 106
22. C.H. Lee, K. Saksela, U.A. Mirza, B.T. Chait, and J. Kuriyan Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain Cell 85 1996 931 942 (Pubitemid 26192143)
23. L.X. Liu, N. Heveker, O.T. Fackler, S. Arold, S. Le Gall, K. Janvier, B.M. Peterlin, C. Dumas, O. Schwartz, S. Benichou, and R. Benarous Mutation of a conserved residue (D123) required for oligomerization of human immunodeficiency virus type 1 Nef protein abolishes interaction with human thioesterase and results in impairment of Nef biological functions J. Virol. 74 2000 5310 5319 (Pubitemid 30313873)
24. M.H. Malim, and M. Emerman HIV-1 accessory proteins - ensuring viral survival in a hostile environment Cell Host Microbe 3 2008 388 398 (Pubitemid 351784365)
25. M. Murphy, L. Jason-Moller, and J. Bruno Using Biacore to measure the binding kinetics of an antibody-antigen interaction Curr. Protoc. Protein Sci. 2006 Chapter 19, Unit 19.14
26. P.S. Narute, and T.E. Smithgall Nef alleles from all major HIV-1 clades activate Src-family kinases and enhance HIV-1 replication in an inhibitor-sensitive manner PLoS One 7 2012 e32561
27. E. O'Neill, L.S. Kuo, J.F. Krisko, D.R. Tomchick, J.V. Garcia, and J.L. Foster Dynamic evolution of the human immunodeficiency virus type 1 pathogenic factor, Nef J. Virol. 80 2006 1311 1320 (Pubitemid 43134088)
28. E.J. Platt, K. Wehrly, S.E. Kuhmann, B. Chesebro, and D. Kabat Effects of CCR5 and CD4 cell surface concentrations on infections by macrophagetropic isolates of human immunodeficiency virus type 1 J. Virol. 72 1998 2855 2864 (Pubitemid 28175522)
29. J.A. Poe, and T.E. Smithgall HIV-1 Nef dimerization is required for Nef-mediated receptor downregulation and viral replication J. Mol. Biol. 394 2009 329 342
30. R.D. Salter, D.N. Howell, and P. Cresswell Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids Immunogenetics 21 1985 235 246 (Pubitemid 15117182)
31. M.F. Sanner Python: a programming language for software integration and development J. Mol. Graph. Model. 17 1999 57 61 (Pubitemid 30029318)
32. A.L. Seman, W.F. Pewen, L.F. Fresh, L.N. Martin, and M. Murphey-Corb The replicative capacity of rhesus macaque peripheral blood mononuclear cells for simian immunodeficiency virus in vitro is predictive of the rate of progression to AIDS in vivo J. Gen. Virol. 81 2000 2441 2449
33. C.A. Spina, T.J. Kwoh, M.Y. Chowers, J.C. Guatelli, and D.D. Richman The importance of nef in the induction of human immunodeficiency virus type 1 replication from primary quiescent CD4 lymphocytes J. Exp. Med. 179 1994 115 123 (Pubitemid 24012976)
34. Z. Temesgen, D. Warnke, and M.J. Kasten Current status of antiretroviral therapy Expert Opin. Pharmacother. 7 2006 1541 1554 (Pubitemid 44209599)
35. R.P. Trible, L. Emert-Sedlak, and T.E. Smithgall HIV-1 Nef selectively activates Src family kinases Hck, Lyn, and c-Src through direct SH3 domain interaction J. Biol. Chem. 281 2006 27029 27038 (Pubitemid 44401731)
36. R.P. Trible, L. Emert-Sedlak, T.E. Wales, V. Ayyavoo, J.R. Engen, and T.E. Smithgall Allosteric loss-of-function mutations in HIV-1 Nef from a long-term non-progressor J. Mol. Biol. 374 2007 121 129 (Pubitemid 47600232)
37. O. Trott, and A.J. Olson AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading J. Comput. Chem. 31 2010 455 461
38. J. Vermeire, G. Vanbillemont, W. Witkowski, and B. Verhasselt The Nef-infectivity enigma: mechanisms of enhanced lentiviral infection Curr. HIV Res. 9 2011 474 489
39. H. Ye, H.J. Choi, J. Poe, and T.E. Smithgall Oligomerization is required for HIV-1 Nef-induced activation of the Src family protein-tyrosine kinase, Hck Biochemistry 43 2004 15775 15784 (Pubitemid 39665077)
40. J.H. Zhang, T.D.Y. Chung, and K.R. Oldenburg A simple statistical parameter for use in evaluation and validation of high throughput screening assays J. Biomol. Screen. 4 1999 67 73 (Pubitemid 29278954)