Quantitative aerobic physiology of the yeast Dekkera bruxellensis, A major contaminant in bioethanol production plants

FEMS Yeast Research

Volumn 13, Issue 1, 2013, Pages 34-43

  Leite, Fernanda Cristina Bezerra ^a   Basso, Thiago Olitta ^b   Pita, Will de Barros ^a   Gombert, Andreas Karoly ^b   Simões, Diogo Ardaillon ^a   De Morais, Marcos Antonio ^a  

^a FEDERAL UNIVERSITY OF PERNAMBUCO   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Carbon limited chemostat; Crabtree effect; Dekkera bruxellensis; Ethanol and acetate yields; Yeast physiology

Indexed keywords

BIOETHANOL; BIOFUEL; DISACCHARIDE; GLUCOSE; HEXOSE; SUCROSE; UNCLASSIFIED DRUG; ACETIC ACID DERIVATIVE; ALCOHOL; BETA FRUCTOFURANOSIDASE; CARBON; CULTURE MEDIUM; FUNGAL PROTEIN; OXYGEN;

ARTICLE; BIOFUEL PRODUCTION; BIOMASS; CARBON SOURCE; CHEMOSTAT; DEKKERA; DEKKERA BRUXELLENSIS; FUNGAL CONTAMINATION; FUNGAL METABOLISM; FUNGAL STRAIN; FUNGUS GROWTH; GROWTH RATE; NONHUMAN; STOICHIOMETRY; AEROBIC METABOLISM; CELL RESPIRATION; CHEMISTRY; CULTURE MEDIUM; FERMENTATION; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MICROBIOLOGY; PHYSIOLOGY; SACCHAROMYCES CEREVISIAE;

DEKKERA BRUXELLENSIS;

ACETATES; AEROBIOSIS; BETA-FRUCTOFURANOSIDASE; BIOMASS; CARBON; CELL RESPIRATION; CULTURE MEDIA; DEKKERA; ETHANOL; FERMENTATION; FUNGAL PROTEINS; GLUCOSE; INDUSTRIAL MICROBIOLOGY; OXYGEN; SACCHAROMYCES CEREVISIAE; SUCROSE;

EID: 84872402050     PISSN: 15671356     EISSN: 15671364     Source Type: Journal    
DOI: 10.1111/1567-1364.12007     Document Type: Article

References (40)

1. Aguilar Uscanga M, Delia ML & Strehaiano P (2003) Brettanomyces bruxellensis: effect of oxygen on growth and acetic acid production. Appl Microbiol Biotechnol 61: 157-162.
2. Basílio ACM, de Araújo PRL, de Morais JOF, Da Silva Filho E, de Morais M & Simões D (2008) Detection and identification of wild yeast contaminants of the industrial fuel ethanol fermentation process. Curr Microbiol 56: 322-326.
3. Basso TO, Dario MG, Tonso A, Stambuk BU & Gombert AK (2010) Insufficient uracil supply in fully aerobic chemostat cultures of Saccharomyces cerevisiae leads to respiro-fermentative metabolism and double nutrient-limitation. Biotechnol Lett 32: 973-977.
4. Bellaver LH, Carvalho NMB, Abrahão Neto J & Gombert AK (2004) Ethanol formation and enzyme activities around glucose 6 phosphate in Kluyveromyces marxianus CBS 6556 exposed to glucose or lactose excess. FEMS Yeast Res 4: 691-698.
5. Blomqvist J, Eberhard T, Schnürer J & Passoth V (2010) Fermentation characteristics of Dekkera bruxellensis strains. Appl Microbiol Biotechnol 87: 1487-1497.
6. Brandam CBC, Castro-Martínez CCMC, Marie-Line Délia MLD, Ramón-Portugal FRPF & Strehaiano PSP (2007) Effect of temperature on Brettanomyces bruxellensis: metabolic and kinetic aspects. Can J Microbiol 54: 11-18.
7. Carnicer M, Baumann K, Töplitz I, Sánchez-Ferrando F, Mattanovich D, Ferrer P & Albiol J (2009) Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels. Microb Cell Fact 8: 65.
8. Conterno L, Joseph CML, Arvik TJ, Henick-Kling T & Bisson LF (2006) Genetic and physiological characterization of Brettanomyces bruxellensis strains isolated from wines. Am J Enol Vitic 57: 139-147.
9. Da Silva Filho EA, De Melo HF, Antunes DF, Dos Santos SK, Do Monte Resende A, Simões DA & de Morais MA Jr (2005) Isolation by genetic and physiological characteristics of a fuel-ethanol fermentative Saccharomyces cerevisiae strain with potential for genetic manipulation. J Ind Microbiol Biotechnol 32: 481-486.
10. De Barros Pita W, Leite FCB, De Souza Liberal AT, Simões DA & De Morais MA (2011) The ability to use nitrate confers advantage to Dekkera bruxellensis over S. cerevisiae and can explain its adaptation to industrial fermentation processes. Antonie Van Leeuwenhoek 100: 99-107.
11. De Souza Liberal AT, Basílio AC, Do Monte Resende A, Brasileiro BT, Da Silva-Filho EA, de Morais JO, Simões DA & De Morais Jr MA (2007) Identification of Dekkera bruxellensis as a major contaminant yeast in continuous fuel ethanol fermentation. J Appl Microbiol 102: 538-547.
12. Diderich JA, Schepper M, Van Hoek P et al. (1999) Glucose uptake kinetics and transcription of HXT genes in chemostat cultures of Saccharomyces cerevisiae. J Biol Chem 274: 15350-15359.
13. Galafassi S, Merico A, Pizza F, Hellborg L, Molinari F, Piskur J & Compagno C (2011) Dekkera/Brettanomyces yeasts for ethanol production from renewable sources under oxygen limited and low-pH conditions. J Ind Microbiol Biotechnol 38: 1079-1088.
14. Hellinga C (1992) Macrobal 2.02. Delft University of Technology, Delft, The Netherlands. Available at http://www.tnw.tudelft.nl/en/about-faculty/departments/biotechnology/research/download-centre/macrobal/.
15. Jansen ML, Diderich JA, Mashego M, Hassane A, de Winde JH, Daran-Lapujade P & Pronk JT (2005) Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity. Microbiology 151: 1657-1669.
16. Jensen SL, Umiker NL, Arneborg N & Edwards CG (2009) Identification and characterization of Dekkera bruxellensis, Candida pararugosa and Pichia guilliermondii isolated from commercial red wines. Food Microbiol 26: 915-921.
17. Kiers J, Zeeman AM, Luttik M et al. (1998) Regulation of alcoholic fermentation in batch and chemostat cultures of Kluyveromyces lactis CBS 2359. Yeast 14: 459-469.
18. Kresnowati MT, van Winden WA, Almering MJ, ten Pierick A, Ras C, Knijnenburg TA, Daran-Lapujade P, Pronk JT, Heijnen JJ & Daran JM (2006) When transcriptome meets metabolome: fast cellular responses of yeast to sudden relief of glucose limitation. Mol Syst Biol 2: 49.
19. Lange HC & Heijnen JJ (2001) Statistical reconciliation of the elemental and molecular biomass composition of Saccharomyces cerevisiae. Biotechnol Bioeng 75: 334-344.
20. Loureiro V & Malfeito-Ferreira M (2003) Spoilage yeasts in the wine industry. Int J Food Microbiol 86: 23-50.
21. Mashego M, Van Gulik W, Vinke J & Heijnen J (2003) Critical evaluation of sampling techniques for residual glucose determination in carbon limited chemostat culture of Saccharomyces cerevisiae. Biotechnol Bioeng 83: 395-399.
22. Olsson L & Nielsen J (1997) On-line and in situ monitoring of biomass in submerged cultivations. Trends Biotechnol 15: 517-522.
23. Passoth V, Blomqvist J & Schnurer J (2007) Dekkera bruxellensis and Lactobacillus vini form a stable ethanol-producing consortium in a commercial alcohol process. Appl Environ Microbiol 73: 4354-4356.
24. Pereira LF, Bassi AP, Avansini SH, Neto AG, Brasileiro BT, Ceccato-Antonini SR & De Morais MA Jr (2012) The physiological characteristics of the yeast Dekkera bruxellensis in fully fermentative conditions with cell recycling and in mixed cultures with Saccharomyces cerevisiae. Antonie Van Leeuwenhoek 101: 529-539.
25. Procházka E, Poláková S, Piškur J & Sulo P (2010) Mitochondrial genome from the facultative anaerobe and petite positive yeast Dekkera bruxellensis contains the NADH dehydrogenase subunit genes. FEMS Yeast Res 10: 545-557.
26. Rozpedowska E, Hellborg L, Ishchuk OP et al. (2011) Parallel evolution of the make-accumulate-consume strategy in Saccharomyces and Dekkera yeasts. Nat Commun 2: 302.
27. Silveira M, Carvajal E & Bon E (1996) Assay for in vivo yeast invertase activity using NaF. Anal Biochem 238: 26-28.
28. Stambuk BU (1999) A simple experiment illustrating metabolic regulation: induction versus repression of yeast α-glucosidase. Biochem Educ 27: 177-180.
29. Van der Heijden R, Romein B, Heijnen J, Hellinga C & Luyben K (1994) Linear constraint relations in biochemical reaction systems: II. Diagnosis and estimation of gross errors. Biotechnol Bioeng 43: 11-20.
30. van Dijken JP & Scheffers WA (1986) Redox balances in the metabolism of sugars by yeasts. FEMS Microbiol Rev 32: 199-224.
31. van Maris AJA, Luttik MAH, Winkler AA, van Dijken JP & Pronk JT (2003) Overproduction of threonine aldolase circumvents the biosynthetic role of pyruvate decarboxylase in glucose-limited chemostat cultures of Saccharomyces cerevisiae. Appl Environ Microbiol 69: 2094-2099.
32. Van Urk H, Mark PR, Scheffers WA & Van Dijken JP (1988) Metabolic responses of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 upon transition from glucose limitation to glucose excess. Yeast 4: 283-291.
33. Van Urk H, Voll WS, Scheffers WA & Van Dijken JP (1990) Transient-state analysis of metabolic fluxes in crabtree-positive and crabtree-negative yeasts. Appl Environ Microbiol 56: 281-287.
34. Vemuri GN, Eiteman MA, McEwen JE, Olsson L & Nielsen J (2007) Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae. P Natl Acad Sci USA 104: 2402-2407.
35. Verduyn C, Postma E, Scheffers WA & van Dijken JP (1990) Energetics of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures. J Gen Microbiol 136: 405-412.
36. Verduyn C, Stouthamer AH, Scheffers WA & Dijken JP (1991) A theoretical evaluation of growth yields of yeasts. Antonie Van Leeuwenhoek 59: 49-63.
37. Verduyn C, Postma E, Scheffers WA & Van Dijken JP (1992) Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8: 501-517.
38. Visser D, van Zuylen GA, van Dam JC et al. (2004) Analysis of in vivo kinetics of glycolysis in aerobic Saccharomyces cerevisiae by application of glucose and ethanol pulses. Biotechnol Bioeng 88: 157-167.
39. Wijsman MR, Dijken JP, Kleeff BHA & Scheffers WA (1984) Inhibition of fermentation and growth in batch cultures of the yeast Brettanomyces intermedius upon a shift from aerobic to anaerobic conditions (Custers effect). Antonie Van Leeuwenhoek 50: 183-192.
40. Woolfit M, Rozpedowska E, Piskur J & Wolfe KH (2007) Genome survey sequencing of the wine spoilage yeast Dekkera (Brettanomyces) bruxellensis. Eukaryot Cell 6: 721.