Overproduction of threonine aldolase circumvents the biosynthetic role of pyruvate decarboxylase in glucose-limited chemostat cultures of Saccharomyces cerevisiae

Applied and Environmental Microbiology

Volumn 69, Issue 4, 2003, Pages 2094-2099

  Van Maris, Antonius J A ^a   Luttik, Marijke A H ^a   Winkler, Aaron A ^b   Van Dijken, Johannes P ^a,b   Pronk, Jack T ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^b Bird Engineering BV   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

COENZYMES; ETHANOL; FRACTIONATION; GLUCOSE; GROWTH KINETICS;

CYTOSOLIC LOCALIZATION;

MICROBIOLOGY;

ACETALDEHYDE; ACETYL COENZYME A; CARBON; CARNITINE; FRUCTOSE BISPHOSPHATE ALDOLASE; GLUCOSE; GLYCINE; PYRUVATE DECARBOXYLASE; THREONINE; THREONINE ALDOLASE; UNCLASSIFIED DRUG;

ENZYME; MICROBIOLOGY;

ARTICLE; CHEMOSTAT; CONCENTRATION RESPONSE; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME LOCALIZATION; ENZYME SYNTHESIS; FUNGAL STRAIN; FUNGUS CULTURE; FUNGUS GROWTH; FUNGUS MUTANT; GENE OVEREXPRESSION; NONHUMAN; SACCHAROMYCES CEREVISIAE;

ACETYL COENZYME A; CARNITINE; CULTURE MEDIA; CYTOSOL; GENE EXPRESSION REGULATION, FUNGAL; GENETIC ENGINEERING; GLUCOSE; GLYCINE HYDROXYMETHYLTRANSFERASE; PYRUVATE DECARBOXYLASE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

FUNGI; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 0037394829     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.4.2094-2099.2003     Document Type: Article

References (41)

1. Atomi, H., M. Ueda, J. Suzuki, Y. Kamada, and A. Tanaka. 1993. Presence of carnitine acyltransferase in peroxisomes and in mitochondria of oleic acid-grown Saccharomyces cerevisiae. FEMS Microbiol. Lett. 112:31-34.
2. Bianchi, M. M., L. Tizzani, M. Destruelle, L. Frontali, and M. WésolowskiLouvel. 1996. The "petite-negative" yeast Kluyveromyces lactis has a single gene expressing pyruvate decarboxylase activity. Mol. Microbiol. 19:27-36.
3. Chen, S., J. S. Brockenbrough, J. E. Dove, and J. P. Aris. 1997. Homocitrate synthase is located in the nucleus in the yeast Saccharomyces cerevisiae. J. Biol. Chem 272:10839-10846.
4. Diderich, J. A., L. M. Raamsdonk, A. Kuiper, A. L. Kruckeberg, M. J. Teixeira de Mattos, and K. van Dam. 2002. Effects of a hexokinase II deletion on the dynamics of glycolysis in continuous cultures of Saccaromyces cerevisiae. FEMS Yeast Res. 2:165-172.
5. Douma, A. C., M. Veenhuis, W. de Koning, M. Evers, and W. Harder. 1985. Dihydroxyacetone synthase is localized in the peroxisomal matrix of methanol-grown Hansenula polymorpha. Arch. Microbiol. 143:237-243.
6. Ehrlich, F. 1907. Über die Bedingungen der Fuselölbindung und über ihren Zusammenhang mit dem Eiweissenaufbau der Hefe. Ber. Deutsch. Chem. Ges. 40:1027-1047.
7. Elgersma, Y., C. W. T. van Roermund, R. J. A. Wanders, and H. F. Tabak. 1995. Peroxisomal and mitochondrial carnitine acetyltransferases of Saccharomyces cerevisiae are encoded by a single gene. EMBO J. 14:3472-3479.
8. Evans, C. T., A. H. Scragg, and C. Ratledge. 1983. A comparative study of citrate efflux from mitochondria of oleaginous and non-oleaginous yeasts. Eur. J. Biochem. 130:195-204.
9. Feller, A., F. Ramos, A. Piérard, and E. Dubois. 1999. In Saccharomyces cerevisiae, feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p. Eur. J. Biochem. 261:163-170.
10. Flikweert, M. T., M. de Swaaf, J. P. van Dijken, and J. T. Pronk. 1999. Growth requirements of pyruvate-decarboxylase-negative Saccharomyces cerevisiae. FEMS Microbiol. Lett. 174:73-79.
11. Flikweert, M. T., M. Kuyper, A. J. A. van Maris, P. Kötter, J. P. van Dijken, and J. T. Pronk. 1999. Steady-state and transient-state analysis of growth and metabolite production in a Saccharomyces cerevisiae strain with reduced pyruvate-decarboxylase activity. Biotechnol. Bioeng. 66:42-50.
12. Flikweert, M. T., L. van der Zanden, W. M. T. M. Janssen, H. Y. Steensma, J. P. van Dijken, and J. T. Pronk. 1996. Pyruvate decarboxylase: an indispensable enzyme for growth of Saccharomyces cerevisiae on glucose. Yeast 12:247-257.
13. Flikweert, M. T., J. P. van Dijken, and J. T. Pronk. 1997. Metabolic response of pyruvate-decarboxylase-negative Saccharomyces cerevisiae to glucose excess. Appl. Environ. Microbiol. 63:3399-3404.
14. Flores, C.-L., C. Rodriguez, T. Petit, and C. Gancedo. 2000. Carbohydrate and energy-yielding metabolism in non-conventional yeasts. FEMS Microbiol. Rev. 24:507-529.
15. Gietz, R. D., and A. Sugino. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-534.
16. Hans, M. A., E. Heinzle, and C. Wittmann. 2001. Quantification of intracellular amino acids in batch cultures of Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 26:776-779.
17. Holzer, H., and W. H. Goedde. 1957. Zwei Wege von Pyruvat zu Acetylcoenzym A in Hefe. Biochem. Z. 329:175-191.
18. Kastanos, E. K., Y. Y. Woldman, and D. R. Appling. 1997. Role of mitochondrial and cytoplasmic serine hydroxymethyltransferase isoenzymes in de novo purine synthesis in Saccharomyces cerevisiae. Biochemistry 36:14956-14964.
19. Kohlhaw, G. B., and A. Tan-Wilson. 1977. Carnitine acetyltransferase: candidate for the transfer of acetyl groups through the mitochondrial membrane of yeast. J. Bacteriol. 129:1159-1161.
20. Lievense, J. C., J. M. Modak, and H. C. Lim. 1989. A mathematical model for the asymmetric dynamic response in microbial systems. J. Biotechnol. 11:49-66.
21. Liu, J.-Q., S. Nagata, T. Dairi, H. Misono, S. Shimizu, and H. Yamada. 1997. The GLY1 gene of Saccharomyces cerevisiae encodes a low-specific L-threonine aldolase that catalyzes cleavage of L-allo-threonine and L-threonine to glycine. Eur. J. Biochem. 245:289-293.
22. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
23. Luttik, M. A. H., K. M. Overkamp, P. Kötter, S. de Vries, J. P. van Dijken, and J. T. Pronk. 1998. The Saccharomyces cerevisiae NDE1 and NDE2 genes encode seperate mitochondrial NADH dehydrogenases catalyzing the oxidation of cytosolic NADH. J. Biol. Chem. 273:24529-24534.
24. Messenguy, F., D. Colin, and J. P. ten Have. 1980. Regulation of compartmentation of amino acid pools in Saccharomyces cerevisiae and its effects on metabolic control. Eur. J. Biochem. 180:439-447.
25. Monschau, N., K.-P. Stahmann, H. Sahm, J. B. Neil, and A. L. Bognar. 1997. Identification of Saccharomyces cerevisiae GLY1 as a threonine aldolase: a key enzyme in glycine biosynthesis. FEMS Microbiol. Lett. 150:55-60.
26. Oura, E. 1972. Reactions leading to the formation of yeast cell material from glucose and ethanol. Ph.D. thesis. University of Helsinki, Helsinki, Finland.
27. Palmieri, L., F. M. Lasorsa, V. Iacobazzi, M. J. Runswick, F. Palmieri, and J. E. Walker. 1999. Identification of the mitochondrial carnitine carrier in Saccharomyces cerevisiae. FEBS Lett. 462:472-476.
28. Piper, M. D., S. P. Hong, G. E. Ball, and I. W. Dawes. 2000. Regulation of the balance of one-carbon metabolism in Saccharomyces cerevisiae. J. Biol. Chem. 275:30987-30995.
29. Porro, D., L. Brambilla, B. M. Ranzi, E. Martegani, and L. Alberghina. 1995. Development of metabolically engineered Saccharomyces cerevisiae cells for the production of lactic acid. Biotechnol. Prog. 11:294-298.
30. Postma, E., C. Verduyn, W. A. Scheffers, and J. P. van Dijken. 1989. Enzymic analysis of the Crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae. Appl. Environ. Microbiol. 53:468-477.
31. Pronk, J. T., H. Y. Steensma, and J. P. van Dijken. 1996. Pyruvate metabolism in Saccharomyces cerevisiae. Yeast 12:1607-1633.
32. Pronk, J. T., T. J. Wenzel, M. A. H. Luttik, C. C. M. Klaassen, W. A. Scheffers, and J. P. van Dijken. 1994. Energetic aspects of glucose metabolism in a pyruvate-dehydrogenase-negative mutant of Saccharomyces cerevisiae. Microbiol. 140:601-610.
33. Ratledge, C., and C. T. Evans. 1989. Lipids and their metabolism, p. 367-455. In A. H. Rose and J. S. Harrison (ed.), The yeasts. vol. 3. Academic Press, San Diego, Calif.
34. Schaaff, I., J. B. A. Green, D. Gozalbo, and S. Hohmann. 1989. A deletion of the PDC1 gene for pyruvate decarboxylase of yeast causes a different phenotype than previously isolated point mutations. Curr. Genet. 15:75-81.
35. Schmitt, H. D., and F. K. Zimmermann. 1982. Genetic analysis of the pyruvate decarboxylase reaction in yeast glycolysis. J. Bacteriol. 151:1146-1152.
36. Swiegers, J. H., N. Dippenaar, I. S. Pretorius, and F. F. Bauer. 2001. Carnitine-dependent metabolic activities in Saccharomyces cerevisiae: three carnitine acyltransferases are essential in a carnitine-dependent strain. Yeast 18:585-595.
37. ter Linde, J. J., H. Liang, R. W. Davis, H. Y. Steensma, J. P. van Dijken, and J. T. Pronk. 1999. Genome-wide transcriptional analysis of aerobic and anaerobic chemostat cultures of Saccharomyces cerevisiae. J. Bacteriol. 181:7409-7413.
38. van Dijken, J. P., J. Bauer, L. Brambilla, P. Duboc, J. M. Francois, C. Gancedo, M. L. F. Giuseppin, J. J. Heijnen, M. Hoare, H. C. Lange, E. A. Madden, P. Niederberger, J. Nielsen, J. L. Parrou, T. Petit, D. Porro, M. Reuss, N. van Riel, M. Rizzi, H. Y. Steensma, C. T. Verrips, J. Vindeløv, and J. T. Pronk. 2000. An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains. Enzyme Microb. Technol. 26:706-714.
39. van Roermund, C. W. T., E. H. Hettema, M. van den Berg, H. F. Tabak, and R. J. A. Wanders. 1999. Molecular characterization of carnitine dependent transport of acetyl-CoA from peroxisomes to mitochondria in Saccharomyces cerevisiae and identification of a plasma membrane carnitine transporter, Agp2. EMBO J. 18:5843-5852.
40. van Urk, H., P. R. Mak, W. A. Scheffers, and J. P. van Dijken. 1988. Metabolic responses of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 upon transition from glucose limitation to glucose excess. Yeast 4:283-291.
41. Verduyn, C., T. P. L. Zomerdijk, J. P. van Dijken, and W. A. Scheffers. 1992. Effect of benzoic acid on metabolic fluxes in yeasts: a continuous culture study on the regulation of respiration and alcoholic fermentation. Yeast 8:501-517.