Debottlenecking recombinant protein production in Bacillus megaterium under large-scale conditions-targeted precursor feeding designed from metabolomics

Biotechnology and Bioengineering

Volumn 109, Issue 6, 2012, Pages 1538-1550

  Korneli, Claudia ^a   Bolten, Christoph Josef ^a   Godard, Thibault ^a   Franco Lara, Ezequiel ^a   Wittmann, Christoph ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

Author keywords

Central metabolism; Dynamics; Free amino acids; GFP; Scale down

Indexed keywords

ASPARTATES; BACILLUS MEGATERIUM; BUILDING BLOCKES; CARBON LOSS; DEBOTTLENECKING; FEED SOLUTION; FEEDING CONDITIONS; FREE AMINO ACIDS; GFP; GREEN FLUORESCENT PROTEIN; IN-LINE; MEGATERIUM; METABOLOMICS; NUTRIENT SUPPLY; OVERFLOW METABOLISM; PRODUCTION SCALE; PYRUVATES; RECOMBINANT MICROORGANISM; RECOMBINANT PROTEIN PRODUCTIONS; RECOMBINANT STRAINS; SCALE DOWN; SMALL-SCALE PROCESS;

BACTERIOLOGY; CARBON DIOXIDE; DYNAMICS; METABOLISM; PHYSIOLOGY; RECOMBINANT PROTEINS; VOLATILE FATTY ACIDS;

AMINO ACIDS;

ACETIC ACID; ALANINE; AMINO ACID; ASPARTIC ACID; CARBON; CARBON DIOXIDE; GLUTAMINE; GREEN FLUORESCENT PROTEIN; HISTIDINE; LYSINE; RECOMBINANT PROTEIN; TRYPTOPHAN;

ARTICLE; BACILLUS MEGATERIUM; BACTERIAL METABOLISM; CONTROLLED STUDY; FEEDING; FILTRATION; METABOLOMICS; NONHUMAN; NUTRIENT SUPPLY; QUANTITATIVE ANALYSIS; VALIDATION PROCESS;

ACETATES; AMINO ACIDS; BACILLUS MEGATERIUM; BIOREACTORS; BIOTECHNOLOGY; CARBON; CARBON DIOXIDE; CULTURE MEDIA; GREEN FLUORESCENT PROTEINS; METABOLOMICS; RECOMBINANT PROTEINS;

BACILLUS MEGATERIUM;

EID: 84859891750     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24434     Document Type: Article

References (47)

1. Ahlawat S, Dhiman SS, Battan B, Mandhan RP, Sharma J. 2009. Pectinase production by Bacillus subtilis and its potential application in biopreparation of cotton and micropoly fabric. Process Biochem 44(5): 521- 526.
2. Akashi H, Gojobori T. 2002. Metabolic efficiency and amino acid composition in the proteomes of Escherichia coli and Bacillus subtilis Proc Natl Acad Sci USA 99(6): 3695- 3700.
3. Ball WJ, Jr, Atkinson DE. 1975. Adenylate energy charge in Saccharomyces cerevisiae during starvation. J Bacteriol 121(3): 975- 982.
4. Becker J, Klopprogge C, Schröder H, Wittmann C. 2009. Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum Appl Environ Microbiol 75(24): 7866- 7869.
5. Biedendieck R, Borgmeier C, Bunk B, Stammen S, Scherling C, Meinhardt F, Wittmann C, Jahn D. 2011. Systems biology of recombinant protein production using Bacillus megaterium Methods Enzymol 500: 165- 195.
6. Bolten CJ, Kiefer P, Letisse F, Portais J-C, Wittmann C. 2007. Sampling for metabolome analysis of microorganisms. Anal Chem 79(10): 3843- 3849.
7. Bylund F, Guillard F, Enfors SO, Trägårdh C, Larsson G. 1999. Scale down of recombinant protein production: A comparative study of scaling performance. Bioprocess Biosyst Eng 20(5): 377- 389.
8. Bylund F, Collet E, Enfors SO, Larsson G. 1998. Substrate gradient formation in the large-scale bioreactor lowers cell yield and increases by-product formation. Bioprocess Biosyst Eng 18(3): 171- 180.
9. Chapman AG, Fall L, Atkinson DE. 1971. Adenylate energy charge in Escherichia coli during growth and starvation. J Bacteriol 108(3): 1072- 1086.
10. Cheng JJ, Timilsina GR. 2011. Status and barriers of advanced biofuel technologies: A review. Renewable Energy 36(12): 3541- 3549.
11. Cormack BP, Valdivia RH, Falkow S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173(1 Spec No): 33- 38.
12. Cutayar JM, Poillon D. 1989. High cell density culture of Escherichia coli in a fed-batch system with dissolved oxygen as substrate feed indicator. Biotechnol Lett 11(3): 155- 160.
13. David F, Westphal R, Bunk B, Jahn D, Franco-Lara E. 2010. Optimization of antibody fragment production in Bacillus megaterium: The role of metal ions on protein secretion. J Biotechnol 150(1): 115- 124.
14. Enfors SO, Jahic M, Rozkov A, Xu B, Hecker M, Jürgen B, Krüger E, Schweder T, Hamer G, O'Beirne D, Noisommit-Rizzi N, Reuss M, Boone L, Hewitt C, McFarlane C, Nienow A, Kovacs T, Trägårdh C, Fuchs L, Revstedt J, Friberg PC, Hjertager B, Blomsten G, Skogman H, Hjort S, Hoeks F, Lin HY, Neubauer P, van der Lans R, Luyben K, Vrabel P, Manelius A. 2001. Physiological responses to mixing in large scale bioreactors. J Biotechnol 85(2): 175- 185.
15. Enjalbert B, Jourdan F, Portais JC. 2011. Intuitive visualization and analysis of multi-omics data and application to Escherichia coli carbon metabolism. PLoS One 6(6): e21318.
16. Hans MA, Heinzle E, Wittmann C. 2003. Free intracellular amino acid pools during autonomous oscillations in Saccharomyces cerevisiae Biotechnol Bioeng 82(2): 143- 151.
17. Hebeda RE, Styrlund CR, Teague WM. 1988. Benefits of Bacillus megaterium amylase in dextrose production. Starch 40(1): 33- 36.
18. Hensing MC, Rouwenhorst RJ, Heijnen JJ, van Dijken JP, Pronk JT. 1995. Physiological and technological aspects of large-scale heterologous-protein production with yeasts. Antonie Van Leeuwenhoek 67(3): 261- 279.
19. Heyland J, Fu J, Blank LM, Schmid A. 2011. Carbon metabolism limits recombinant protein production in Pichia pastoris Biotechnol Bioeng 108(8): 1942- 1953.
20. Kar T, Delvigne F, Masson M, Destain J, Thonart P. 2008. Investigation of the effect of different extracellular factors on the lipase production by Yarrowia lipolityca on the basis of a scale-down approach. J Ind Microbiol Biotechnol 35(9): 1053- 1059.
21. Kim BS, Lee SC, Lee SY, Chang YK, Chang HN. 2004. High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat. Bioprocess Biosyst Eng 26(3): 147- 150.
22. Kohlstedt M, Becker J, Wittmann C. 2010. Metabolic fluxes and beyond-systems biology understanding and engineering of microbial metabolism. Appl Microbiol Biotechnol 88(5): 1065- 1075.
23. Korneli C, David F, Godard T, Franco-Lara E. 2011. Influence of fructose and oxygen gradients on fed-batch recombinant protein production using Bacillus megaterium Eng Life Sci 11(4): 338- 349.
24. Krömer JO, Bolten CJ, Heinzle E, Schröder H, Wittmann C. 2008. Physiological response of Corynebacterium glutamicum to oxidative stress induced by deletion of the transcriptional repressor McbR. Microbiology 154(Pt 12): 3917- 3930.
25. Kumar M, Gayen K. 2011. Developments in biobutanol production: New insights. Appl Energy 88(6): 1999- 2012.
26. Lee JW, Kim TY, Jang Y-S, Choi S, Lee SY. 2011. Systems metabolic engineering for chemicals and materials. Trends Biotechnol 29(8): 370- 378.
27. 3). Afr J Biotechnol 9(42): 7186- 7191.
28. Metz RJ, Allen LN, Cao TM, Zeman NW. 1988. Nucleotide sequence of an amylase gene from Bacillus megaterium Nucleic Acids Res 16(11): 5203.
29. Mierau I, Leij P, van Swam I, Blommestein B, Floris E, Mond J, Smid EJ. 2005. Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: The case of lysostaphin. Microb Cell Factories 4: 15.
30. Neubauer P, Junne S. 2010. Scale-down simulators for metabolic analysis of large-scale bioprocesses. Curr Opin Biotechnol 21(1): 114- 121.
31. Oosterhuis NM, Kossen NW, Olivier AP, Schenk ES. 1985. Scale-down and optimization studies of the gluconic acid fermentation by Gluconobacter oxydans Biotechnol Bioeng 27(5): 711- 720.
32. Park SJ, Lee SY, Cho J, Kim TY, Lee JW, Park JH, Han MJ. 2005. Global physiological understanding and metabolic engineering of microorganisms based on omics studies. Appl Microbiol Biotechnol 68(5): 567- 579.
33. Ramírez DM, Bentley WE. 1995. Fed-batch feeding and induction policies that improve foreign protein synthesis and stability by avoiding stress responses. Biotechnol Bioeng 47(5): 596- 608.
34. Reiling HE, Laurila H, Fiechter A. 1985. Mass culture of Escherichia coli: Medium development for low and high density cultivation of Escherichia coli B/r in minimal and complex media. J Biotechnol 2(3-4): 191- 206.
35. Rygus T, Hillen W. 1991. Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon. Appl Microbiol Biotechnol 35: 594- 599.
36. Sandoval-Basurto EA, Gosset G, Bolivar F, Ramirez OT. 2005. Culture of Escherichia coli under dissolved oxygen gradients simulated in a two-compartment scale-down system: Metabolic response and production of recombinant protein. Biotechnol Bioeng 89(4): 453- 463.
37. Sauer U, Hatzimanikatis V, Hohmann HP, Manneberg M, Van Loon APGM, Bailey JE. 1996. Physiology and metabolic fluxes of wild-type and riboflavin-producing Bacillus subtilis Appl Environ Microbiol 62(10): 3687- 3696.
38. Shojaosadati SA, Varedi Kolaeil SM, Babaeipour V, Farnoud AM. 2008. Recent advances in high cell density cultivation for production of recombinant protein. Iran J Biotechnol 6(2): 63- 84.
39. Silva RG, Souza VR, Nucci ER, Pinotti LM, Cruz AJG, Giordano RC, Giordano RLC. 2006. Using a medium of free amino acids to produce penicillin G acylase in fed-batch cultivations of Bacillus megaterium ATCC 14945. Braz J Chem Eng 23(1): 37- 43.
40. Stammen S, Müller BK, Korneli C, Biedendieck R, Gamer M, Franco-Lara E, Jahn D. 2010. High-yield intra- and extracellular protein production using Bacillus megaterium Appl Environ Microbiol 76(12): 4037- 4046.
41. Vary PS, Biedendieck R, Fürch T, Meinhardt F, Rohde M, Deckwer WD, Jahn D. 2007. Bacillus megaterium-from simple soil bacterium to industrial protein production host. Appl Microbiol Biotechnol 76(5): 957- 967.
42. Wittchen KD, Meinhardt F. 1995. Inactivation of the major extracellular protease from Bacillus megaterium DSM319 by gene replacement. Appl Microbiol Biotechnol 42: 871- 877.
43. Wittmann C, Weber J, Betiku E, Krömer J, Böhm D, Rinas U. 2007. Response of fluxome and metabolome to temperature-induced recombinant protein synthesis in Escherichia coli J Biotechnol 132(4): 375- 384.
44. Wittmann C, Hans M, van Winden WA, Ras C, Heijnen JJ. 2005. Dynamics of intracellular metabolites of glycolysis and TCA cycle during cell-cycle-related oscillation in Saccharomyces cerevisiae Biotechnol Bioeng 89(7): 839- 847.
45. Wittmann C, Krömer JO, Kiefer P, Binz T, Heinzle E. 2004. Impact of the cold shock phenomenon on quantification of intracellular metabolites in bacteria. Anal Biochem 327(1): 135- 139.
46. Xie T, Fang HY, Zhuge B, Zhuge J. 2009. Promotional mechanism of high glycerol productivity in the aerobic batch fermentation of Candida glycerinogenes after feeding several amino acids. Appl Biochem Microbiol 45(3): 303- 308.
47. Yang Y, Biedendieck R, Wang W, Gamer M, Malten M, Jahn D, Deckwer W-D. 2006. High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium Microb Cell Factory 5: 36. DOI: 10.1186/1475-2859-5-36.