Monitoring of transcriptome and proteome profiles to investigate the cellular response of E. coli towards recombinant protein expression under defined chemostat conditions

Journal of Biotechnology

Volumn 135, Issue 1, 2008, Pages 34-44

  Dürrschmid, Karin ^a   Reischer, Helga ^a   Schmidt Heck, Wolfgang ^b   Hrebicek, Thomas ^c   Guthke, Reinhard ^b   Rizzi, Andreas ^c   Bayer, Karl ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b HANS KNÖLL INSTITUTE   (Germany)

^c UNIVERSITY OF VIENNA   (Austria)

Author keywords

E. coli; Ettan DIGE; MALDI; Microarray; Monitoring; Recombinant proteins; Stress response; Two dimensional gel electrophoresis

Indexed keywords

CHEMOSTATS; ELECTROPHORESIS; ESCHERICHIA COLI; GENE EXPRESSION; METABOLISM; STRESS ANALYSIS;

ADVERSE CONDITIONS; RECOMBINANT PROTEINS; STRESS RESPONSE;

PROTEINS;

AEROBIC RESPIRATION CONTROL PROTEIN A; AEROBIC RESPIRATION CONTROL PROTEIN B; GREEN FLUORESCENT PROTEIN; GUANOSINE 3' DIPHOSPHATE 5' DIPHOSPHATE; ISOPROPYL THIOGALACTOSIDE; NUCLEIC ACID BINDING PROTEIN; PROTEOME; RECOMBINANT PROTEIN; SUPEROXIDE DISMUTASE; TRANSCRIPTOME;

AEROBIC METABOLISM; ARTICLE; CHEMOSTAT; CONTROLLED STUDY; DOWN REGULATION; ESCHERICHIA COLI; GEL ELECTROPHORESIS; MARKER GENE; MICROARRAY ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; OXIDATIVE STRESS; POSTSYNAPTIC POTENTIAL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN TARGETING; PROTON MOTIVE FORCE; REGULATORY MECHANISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS CULTURE;

ALGORITHMS; BIOREACTORS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION PROFILING; MONITORING, PHYSIOLOGIC; PROTEOME; RECOMBINANT PROTEINS; TRANSCRIPTION FACTORS;

ARCA; ESCHERICHIA COLI;

EID: 43049096460     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2008.02.013     Document Type: Article

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