Improved production of monoclonal antibodies through oxygen-limited cultivation of glycoengineered yeast

Journal of Biotechnology

Volumn 155, Issue 2, 2011, Pages 217-224

  Berdichevsky, Marina ^a   d'Anjou, Marc ^b   Mallem, Muralidhar R ^b   Shaikh, Seemab S ^b   Potgieter, Thomas I ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b GlycoFi Incorporated   (United States)

Author keywords

Antibody; DoE; Glycosylation; Oxygen limitation; Pichia pastoris

Indexed keywords

ANTIBODY CONCENTRATION; BIOMASS CONCENTRATIONS; DOE; GALACTOSYLATION; HEAT AND MASS TRANSFER; HEAT REMOVAL RATES; LIMITING FACTORS; METHANOL FEED; N-GLYCAN; NONLINEAR FUNCTIONS; OXYGEN LIMITATION; OXYGEN UPTAKE RATES; P. PASTORIS; PICHIA PASTORIS; PILOT SCALE; SCALE-UP; SPECIFIC GROWTH RATE; SPECIFIC PRODUCTIVITY; STRUCTURE-FUNCTION RELATIONSHIP; THERAPEUTIC PROTEIN; YEAST PROTEINS;

FUNCTIONS; HEAT TRANSFER; MASS TRANSFER; METHANOL; MONOCLONAL ANTIBODIES; PROTEINS; YEAST;

OXYGEN;

GLYCAN; METHANOL; MONOCLONAL ANTIBODY; OXYGEN;

ANTIBODY PRODUCTION; ARTICLE; BIOENGINEERING; BIOMASS PRODUCTION; GLYCOENGINEERING; HEAT; NONHUMAN; OXYGEN CONSUMPTION; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; YEAST;

ANTIBODIES, MONOCLONAL; BIOMASS; BIOREACTORS; HOT TEMPERATURE; METHANOL; OXYGEN CONSUMPTION; PICHIA; POLYSACCHARIDES; PROTEIN ENGINEERING;

PICHIA PASTORIS;

EID: 80051471566     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.06.021     Document Type: Article

References (25)

1. Bailey J., Ollis D. Biochemical Engineering Fundamentals 1986, McGraw-Hill, New York.
2. Baumann K., Maurer M., Dragosits M., Cos O., Ferrer P., Mattanovich D. Hypoxic fed-batch cultivation of Pichia pastoris increases specific and volumetric productivity of recombinant proteins. Biotechnol. Bioeng. 2008, 100:177-183.
3. Birch J.R., Racher A.J. Antibody production. Adv. Drug Deliv. Rev. 2006, 58:671-685.
4. Bobrowicz P., Davidson R.C., Li H., Potgieter T.I., Nett J.H., Hamilton S.R., Stadheim T.A., Miele R.G., Bobrowicz B., Mitchell T., Rausch S., Renfer E., Wildt S. Engineering of an artificial glycosylation pathway blocked in core oligosaccharide assembly in the yeast Pichia pastoris: production of complex humanized glycoproteins with terminal galactose. Glycobiology 2004, 14:757-766.
5. Boettner M., Prinz B., Holz C., Stahl U., Lang C. Highthroughput screening for expression of heterologous proteins in the yeast Pichia pastoris. J. Biotechnol. 2002, 99:51-62.
6. Celik E., Calik P., Oliver S.G. Fed-batch methanol feeding strategy for recombinant protein production by Pichia pastoris in the presence of co-substrate sorbitol. Yeast 2009, 26:473-484.
7. Charoenrat T., Ketudat-Cairns M., Stendahl-Andersen H., Jahic M., Enfors S.O. Oxygen-limited fed-batch process: an alternative control for Pichia pastoris recombinant protein processes. Bioproc. Biosyst. Eng. 2005, 27:399-406.
8. Durocher Y., Butler M. Expression systems for therapeutic glycoprotein production. Curr. Opin. Biotechnol. 2009, 20:700-707.
9. Jahic M., Wallberg F., Bollok M., Garcia P., Enfors S.O. Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures. Microb. Cell Factories 2003, 2.
10. Jiang Y., Li F., Zha D., Potgieter T.I., Mitchell T., Moore R., Cukan M., Houston-Cummings N.R., Nylen A., Drummond J.E., McKelvey T.W., d'Anjou M., Stadheim T.A., Sethuraman N., Li H. Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris. Protein Expr. Purif. 2011, 76:7-14.
11. Jungo C., Schenk J., Pasquier M., Marison I.W., von Stockar U. A quantitative analysis of the benefits of mixed feeds of sorbitol and methanol for the production of recombinant avidin with Pichia pastoris. J. Biotechnol. 2007, 131:57-66.
12. Junker B.H. Scale-up methodologies for Escherichia coli and yeast fermentation processes. J. Biosci. Bioeng. 2004, 97:347-364.
13. Khatri N.K., Hoffmann F. Impact of methanol concentration on secreted protein production in oxygen-limited cultures of recombinant Pichia pastoris. Biotechnol. Bioeng. 2005, 93:871-879.
14. Khatri N.K., Hoffmann F. Oxygen-limited control of methanol uptake for improved production of a single-chain antibody fragment with recombinant Pichia pastoris. Appl. Microbiol. Biotechnol. 2006, 72:492-498.
15. Li H., Sethuraman N., Stadheim T.A., Zha D., Prinz B., Ballew N., Bobrowicz P., Gong B., Hamilton S.R., Hoops J.P., Jiang Y., Kim N., Mansfield R., Nett J.H., Rios S., Strawbridge R., Wildt S., Gerngross T.U. Optimization of humanized IgGs in glycoengineered Pichia pastoris. Nat. Biotechnol. 2006, 24:210.
16. Natsume A., Niwa R., Satoh M. Improving effector functions of antibodies for cancer treatment: enhancing ADCC and CDC. Drug Des. Dev. Ther. 2009, 3:7-16.
17. Potgieter T.I., Kersey S.D., Mallem M.R., Nylen A.C., d'Anjou M. Antibody expression kinetics in glycoengineered Pichia Pastoris. Biotechnol. Bioeng. 2010, 106:918.
18. Potgieter T.I., Cukan M., Drummond J.E., Houston-Cummings N.R., Jiang Y., Li F., Lynaugh h., Mallem M.R., McKelvey T.W., Mitchell T., Nylen A.C., Rittenhour A., Stadheim T.A., Zha D., d'Anjou M. Production of monoclonal antibodies by glycoengineered Pichia pastoris. J. Biotechnol. 2009, 139:318-325.
19. Ramon R., Ferrer P., Valero F. Sorbitol co-feeding reduces metabolic burden caused by the overexpression of a Rhizopus oryzae lipase in Pichia pastoris. J. Biotechnol. 2007, 130:39-46.
20. Rustandi R.R., Washabaugh M.W., Wang Y. Applications of CE SDS gel in development of biopharmaceutical antibody-based products. Electrophoresis 2008, 29:3612-3620.
21. Sinha J., Plantz B.A., Inan M., Meagher M.M. Causes of proteolytic degradation of secreted recombinant proteins produced in methylotrophic yeast Pichia pastoris: case study with recombinant ovine interferon-t. Biotechnol. Bioeng. 2005, 89:102-112.
22. Sinha J., Plantz B.A., Zhang W., Gouthro M., Schlegel V., Liu C.P., Meagher M.M. Improved production of recombinant ovine interferon-t by Mut+ strain of Pichia pastoris using an optimized methanol feed profile. Biotechnol. Bioeng. 2003, 19:794-802.
23. Trentmann O., Khatri N.K., Hoffmann F. Reduced oxygen supply increases process stability and product yield with recombinant Pichia pastoris. Biotechnol. Prog. 2004, 20:1766-1775.
24. Umana P., Jean-Mairet J., Moudry R., et al. Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody dependent cellular cytotoxicity activity. Nat. Biotechnol. 1999, 17:176-180.
25. Walsh G. Biopharmaceutical benchmarks 2010. Nat. Biotechnol. 2010, 28:917-924.