Applications of Microfluidics in Stem Cell Biology

BioNanoScience

Volumn 2, Issue 4, 2012, Pages 277-286

  Zhang, Qiucen ^a   Austin, Robert H ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

Cancer; Microfluidics; Stem cells

Indexed keywords

CANCER; DECISION MAKING PROCESS; HIGH-THROUGHPUT; KEY FEATURE; MICRO-FLUIDIC DEVICES; MICROFLUIDICS TECHNOLOGY; PHYSIOLOGICAL ENVIRONMENT; RECENT PROGRESS; STEM CELL BIOLOGISTS; STEM CELL BIOLOGY; STEM CELL RESEARCH;

CYTOLOGY; FLUIDIC DEVICES; MEDICAL APPLICATIONS; MICROFLUIDICS; RESEARCH; TISSUE CULTURE;

STEM CELLS;

DIMETICONE; REACTIVE OXYGEN METABOLITE;

ARTICLE; BRAIN CORTEX; CELL DIFFERENTIATION; CULTURE MEDIUM; CYTOLOGY; DECISION MAKING; ELECTRONICS; EVOLUTION; FLUORESCENCE MICROSCOPY; HIGH THROUGHPUT SCREENING; HUMAN; MECHANICAL STRESS; MESENCHYMAL STEM CELL; MICROENVIRONMENT; MICROFLUIDIC ANALYSIS; NONHUMAN; STEM CELL; STEM CELL RESEARCH; TISSUE CULTURE;

EID: 84868701701     PISSN: 21911630     EISSN: 21911649     Source Type: Journal    
DOI: 10.1007/s12668-012-0051-8     Document Type: Article

References (113)

1. Whitesides, G. (2006). The origins and the future of microfluidics. Nature, 442(7101), 368.
2. El-Ali, J., Sorger, P., Jensen, K. (2006). Cells on chips. Nature, 442(7101), 403.
3. Austin, R., Tung, C., Lambert, G., Liao, D., Gong, X. (2010). An introduction to micro-ecology patches. Chemical Society Reviews, 39(3), 1049.
4. Weissman, I. (2000). Stem cells: units of development, review Units of regeneration, and units in evolution. Cell, 100, 157.
5. Thomson, J., Itskovitz-Eldor, J., Shapiro, S., Waknitz, M., Swiergiel, J., Marshall, V., Jones, J. (1998). Embryonic stem cell lines derived from human blastocysts. Science, 282(5391), 1145.
6. Fuchs, E., & Segre, J. (2000). Stem cells: review a new lease on life. Cell, 100, 143.
7. Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Scholer, H., Smith, A. (1998). Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell, 95(3), 379.
8. Brignier, A., & Gewirtz, A. (2010). Embryonic and adult stem cell therapy. Journal of Allergy and Clinical Immunology, 125(2), S336.
9. Yamanaka, S. (2007). Strategies and new developments in the generation of patient-specific pluripotent stem cells. Cell Stem Cell, 1(1), 39.
10. Takahashi, K., & Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 126(4), 663.
11. Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., Yamanaka, S. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 131(5), 861.
12. Johansson, C., Momma, S., Clarke, D., Risling, M., Lendahl, U., Frisén, J. (1999). Identification of a neural stem cell in the adult mammalian central nervous system. Cell, 96(1), 25.
13. Jiang, Y., Jahagirdar, B., Reinhardt, R., Schwartz, R., Keene, C., Ortiz-Gonzalez, X., Reyes, M., Lenvik, T., Lund, T., Blackstad, M., et al. (2002). Pluripotency of mesenchymal stem cells derived from adult marrow. Nature, 418(6893), 41.
14. Potten, C., & Loeffler, M. (1990). Stem cells: attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt. Development, 110(4), 1001.
15. Mery, E., Ricoul, F., Sarrut, N., Constantin, O., Delapierre, G., Garin, J., Vinet, F. (2008). A silicon microfluidic chip integrating an ordered micropillar array separation column and a nano-electrospray emitter for LC/MS analysis of peptides. Sensors and Actuators B: Chemical, 134(2), 438.
16. Kalkandjiev, K., Riegger, L., Kosse, D., Welsche, M., Gutzweiler, L., Zengerle, R., Koltay, P. (2011). Microfluidics in silicon/polymer technology as a cost-efficient alternative to silicon/glass. Journal of Micromechanics and Microengineering, 21, 025008.
17. Lin, Y., Yang, C., Wang, C., Chang, F., Huang, K., Hsieh, W. (2012). An aluminum microfluidic chip fabrication using a convenient micromilling process for fluorescent poly (DL-lactide-co-glycolide) microparticle generation. Sensors, 12(2), 1455.
18. Xia, Y., & Whitesides, G. (1998). Soft lithography. Annual Review of Materials Science, 28(1), 153.
19. Unger, M., Chou, H., Thorsen, T., Scherer, A., Quake, S. (2000). Monolithic microfabricated valves and pumps by multilayer soft lithography. Science, 288(5463), 113.
20. Yu, L., Li, C., Liu, Y., Gao, J., Wang, W., Gan, Y. (2009). Flow-through functionalized PDMS microfluidic channels with dextran derivative for ELISAs. Lab Chip, 9(9), 1243.
21. Fiddes, L., Raz, N., Srigunapalan, S., Tumarkan, E., Simmons, C., Wheeler, A., Kumacheva, E. (2010). A circular cross-section PDMS microfluidics system for replication of cardiovascular flow conditions. Biomaterials, 31(13), 3459.
22. Wang, M., Cui, D., Wang, L., Chen, X., Zhao, Q. (2011). Fabrication of microfluidic electrocontrolled chip in polydimethylsiloxane (PDMS). International Journal of Nonlinear Sciences and Numerical Simulation, 3(3-4), 207.
23. Knight, J., Vishwanath, A., Brody, J., Austin, R. (1998). Hydrodynamic focusing on a silicon chip: mixing nanoliters in microseconds. Physical Review Letters, 80(17), 3863.
24. Loutherback, K., Puchalla, J., Austin, R., Sturm, J. (2009). Deterministic microfluidic ratchet. Physical Review Letters, 102(4), 45301.
25. Loutherback, K., Chou, K., Newman, J., Puchalla, J., Austin, R., Sturm, J. (2010). Improved performance of deterministic lateral displacement arrays with triangular posts. Microfluidics and Nanofluidics, 9(6), 1143.
26. Lambert, G., Liao, D., Austin, R. (2010). Collective escape of chemotactic swimmers through microscopic ratchets. Physical Review Letters, 104(16), 168102.
27. Zhang, Q., Lambert, G., Liao, D., Kim, H., Robin, K., Tung, C., Pourmand, N., Austin, R. (2011). Acceleration of emergence of bacterial antibiotic resistance in connected microenvironments. Science, 333(6050), 1764.
28. Hong, J., Quake, S., et al. (2003). Integrated nanoliter systems. Nature Biotechnology, 21(10), 1179.
29. Mark, D., Weber, P., Lutz, S., Focke, M., Zengerle, R., von Stetten, F. (2011). Aliquoting on the centrifugal microfluidic platform based on centrifugo-pneumatic valves. Microfluidics and Nanofluidics, 10(6), 1279.
30. Araci, I., & Quake, S. (2012). Microfluidic very large scale integration (mVLSI) with integrated micromechanical valves. Lab Chip, 12, 2803-2806. doi: 10. 1039/C2LC40258K.
31. Tsai, J., & Lin, L. (2002). Active microfluidic mixer and gas bubble filter driven by thermal bubble micropump. Sensors and Actuators A: Physical, 97, 665.
32. Kannappan, K., Bogle, G., Travas-Sejdic, J., Williams, D. (2011). Computational design of mixers and pumps for microfluidic systems, based on electrochemically-active conducting polymers. Chemical Physics, 13(12), 5450.
33. Lee, C., Chang, C., Wang, Y., Fu, L. (2011). Microfluidic mixing: a review. International Journal of Molecular Sciences, 12(5), 3263.
34. Graydon, O. (2011). Microfluidics: laser-induced bubbles create valves and pumps. Nature Photonics, 5(5), 256.
35. Kim, J., Kang, M., Jensen, E., Mathies, R. (2012). Lifting gate PDMS microvalves and pumps for microfluidic control. Analytical Chemistry doi: 10. 1021/ac202934x.
36. Morrison, S., & Spradling, A. (2008). Stem cells and niches: mechanisms that promote stem cell maintenance throughout life. Cell, 132(4), 598.
37. Barker, N., Ridgway, R., van Es, J., M. van de Wetering, Begthel, H., M. van den Born, Danenberg, E., Clarke, A., Sansom, O., Clevers, H. (2008). Crypt stem cells as the cells-of-origin of intestinal cancer. Nature, 457(7229), 608.
38. Aguiari, P., Leo, S., Zavan, B., Vindigni, V., Rimessi, A., Bianchi, K., Franzin, C., Cortivo, R., Rossato, M., Vettor, R., et al. (2008). High glucose induces adipogenic differentiation of muscle-derived stem cells. Science, 105(4), 1226.
39. Ivanovic, Z. (2009). Hypoxia or in situ normoxia: the stem cell paradigm. Journal of Cellular Physiology, 219(2), 271.
40. De Filippis, L., & ZDelia, D. (2011). Hypoxia in the regulation of neural stem cells. Cellular and Molecular Life Sciences, 68(17), 2831-2844.
41. Gómez-Sjöberg, R., Leyrat, A., Pirone, D., Chen, C., Stephen, R. (2007). Versatile, fully automated, microfluidic cell culture system. Analytical Chemistry, 79(22), 8557.
42. Lecault, V., VanInsberghe, M., Sekulovic, S., Knapp, D., Wohrer, S., Bowden, W., Viel, F., McLaughlin, T., Jarandehei, A., Miller, M., et al. (2011). High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays. Nature Methods, 8(7), 581.
43. Taylor, R., Falconnet, D., Niemistö, A., Ramsey, S., Prinz, S., Shmulevich, I., Galitski, T., Hansen, C. (2009). Dynamic analysis of MAPK signaling using a high-throughput microfluidic single-cell imaging platform. Proceedings of the National Academy of Sciences, 106(10), 3758.
44. Wang, Z., Kim, M., Marquez, M., Thorsen, T. (2007). High-density microfluidic arrays for cell cytotoxicity analysis. Lab Chip, 7(6), 740.
45. Tay, S., Hughey, J., Lee, T., Lipniacki, T., Quake, S., Covert, M. (2010). Single-cell NF-[kgr] B dynamics reveal digital activation and analogue information processing. Nature, 466(7303), 267.
46. Chin, M., Mason, M., Xie, W., Volinia, S., Singer, M., Peterson, C., Ambartsumyan, G., Aimiuwu, O., Richter, L., Zhang, J., et al. (2009). Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures. Cell Stem Cell, 5(1), 111.
47. Kenis, P., Ismagilov, R., Whitesides, G. (1999). Microfabrication inside capillaries using multiphase laminar flow patterning. Science, 285(5424), 83.
48. Takayama, S., Ostuni, E., LeDuc, P., Naruse, K., Ingber, D., Whitesides, G., et al. (2001). Subcellular positioning of small molecules. Nature, 411(6841), 1016.
49. Mark, D., Haeberle, S., Roth, G., Von Stetten, F., Zengerle, R. (2010). Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. Chemical Society Reviews, 39(3), 1153.
50. Oppegard, S., Nam, K., Carr, J., Skaalure, S., Eddington, D. (2009). Modulating temporal and spatial oxygenation over adherent cellular cultures. PLoS One, 4(9), e6891.
51. Toley, B., Park, J., Kim, B., Venkatasubramanian, R., Maharbiz, M., Forbes, N. (2011). Micrometer-scale oxygen delivery rearranges cells and prevents necrosis in tumor tissue in vitro. Biotechnology Progress, 28(2), 515-525.
52. Chung, B., Flanagan, L., Rhee, S., Schwartz, P., Lee, A., Monuki, E., Jeon, N. (2005). Human neural stem cell growth and differentiation in a gradient-generating microfluidic device. Lab Chip, 5(4), 401.
53. Lo, J., Sinkala, E., Eddington, D. (2010). Oxygen gradients for open well cellular cultures via microfluidic substrates. Lab Chip, 10(18), 2394.
54. Lucchetta, E., Lee, J., Fu, L., Patel, N., Ismagilov, R. (2005). Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidics. Nature, 434(7037), 1134.
55. Houchmandzadeh, B., Wieschaus, E., Leibler, S., et al. (2002). Establishment of developmental precision and proportions in the early Drosophila embryo. Nature, 415, 798-801.
56. Jaeger, J., Surkova, S., Blagov, M., Janssens, H., Kosman, D., Kozlov, K., et al. (2004). Dynamic control of positional information in the early Drosophila embryo. Nature, 430(6997), 368.
57. Blagovic, K., Kim, L., Voldman, J. (2011). Microfluidic perfusion for regulating diffusible signaling in stem cells. PloS One, 6(8), e22892.
58. Androutsellis-Theotokis, A., Leker, R., Soldner, F., Hoeppner, D., Ravin, R., Poser, S., Rueger, M., Bae, S., Kittappa, R., McKay, R. (2006). Notch signalling regulates stem cell numbers in vitro and in vivo. Nature, 442(7104), 823.
59. Skelley, A., Kirak, O., Suh, H., Jaenisch, R., Voldman, J. (2009). Microfluidic control of cell pairing and fusion. Nature Methods, 6(2), 147.
60. Park, J., Cho, C., Parashurama, N., Li, Y., Berthiaume, F., Toner, M., Tilles, A., Yarmush, M. (2007). Microfabrication-based modulation of embryonic stem cell differentiation. Lab Chip, 7(8), 1018.
61. Justice, B., Badr, N., Felder, R. (2009). 3D cell culture opens new dimensions in cell-based assays. Drug Discovery Today, 14(1-2), 102.
62. Haycock, J. (2011). 3D cell culture: a review of current approaches and techniques. Methods in Molecular Biology, 695, 1.
63. Huh, D., Hamilton, G., Ingber, D. (2011). Trends in Cell Biology, 21(12), 745-754.
64. Leclerc, E., Sakai, Y., Fujii, T. (2003). Cell culture in 3-dimensional microfluidic structure of PDMS (polydimethylsiloxane). Biomedical Microdevices, 5(2), 109.
65. Albrecht, D., Underhill, G., Wassermann, T., Sah, R., Bhatia, S. (2006). Probing the role of multicellular organization in three-dimensional microenvironments. Nature Methods, 3(5), 369.
66. Toh, Y., Zhang, C., Zhang, J., Khong, Y., Chang, S., Samper, V., van Noort, D., Hutmacher, D., Yu, H. (2007). A novel 3D mammalian cell perfusion-culture system in microfluidic channels. Lab Chip, 7(3), 302.
67. Paguirigan, A., & Beebe, D. (2006). Gelatin-based microfluidic devices for cell culture. Lab Chip, 6(3), 407.
68. Kim, M., Yeon, J., Park, J. (2007). A microfluidic platform for 3-dimensional cell culture and cell-based assays. Biomedical Microdevices, 9(1), 25.
69. Ong, S., Zhang, C., Toh, Y., Kim, S., Foo, H., Tan, C., van Noort, D., Park, S., Yu, H. (2008). A gel-free 3D microfluidic cell culture system. Biomaterials, 29(22), 3237.
70. Reya, T., Morrison, S., Clarke, M., Weissman, I. (2001). Stem cells, cancer, and cancer stem cells. Nature, 414, 105.
71. Singh, S., Clarke, I., Hide, T., Dirks, P. (2004). Cancer stem cells in nervous system tumors. Oncogene, 23(43), 7267.
72. Wang, J., & Dick, J. (2005). Cancer stem cells: lessons from leukemia. Trends in Cell Biology, 15(9), 494.
73. Soltysova, A., Altanerova, V., Altaner, C., et al. (2005). Cancer stem cells. Neoplasma, 52(6), 435.
74. Jordan, C., Guzman, M., Noble, M. (2006). Cancer stem cells. New England Journal of Medicine, 355(12), 1253.
75. Lugo, T., Pendergast, A., Muller, A., Witte, O. (1990). Tyrosine kinase activity and transformation potency of bcr-abl oncogene products. Science, 247(4946), 1079.
76. Rowley, J. (1973). A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by Quinacrine Fluorescence and Giemsa Staining. Nature, 243, 290.
77. Faley, S., Copland, M., Wlodkowic, D., Kolch, W., Seale, K., Wikswo, J., Cooper, J. (2009). Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells. Lab Chip, 9(18), 2659.
78. Zhang, Q., Robin, K., Liao, D., Lambert, G., Austin, R. (2011). The goldilocks principle and antibiotic resistance in bacteria. Molecular Pharmaceutics, 8(6), 2063-2068.
79. Zhang, Q., & Austin, R. (2012). Physics of cancer: the impact of heterogeneity. Annual Review of Condensed Matter Physics, 3, 363-382.
80. Lambert, G., Estévez-Salmeron, L., Oh, S., Liao, D., Emerson, B., Tlsty, T., Austin, R. (2011). An analogy between the evolution of drug resistance in bacterial communities and malignant tissues. Nature Reviews Cancer, 11(5), 375.
81. Perez, O., & Nolan, G. (2006). Phospho-proteomic immune analysis by flow cytometry: from mechanism to translational medicine at the single-cell level. Immunological Reviews, 210(1), 208.
82. Bøyum, A. (1974). Separation of blood leucocytes, granulocytes and lymphocytes. Tissue Antigens, 4(3), 269.
83. Wachtel, S., Sammons, D., Manley, M., Wachtel, G., Twitty, G., Utermohlen, J., Phillips, O., Shulman, L., Taron, D., Müller, U., et al. (1996). Fetal cells in maternal blood: recovery by charge flow separation. Human Genetics, 98(2), 162.
84. Baumgarth, N., & Roederer, M. (2000). A practical approach to multicolor flow cytometry for immunophenotyping. Journal of Immunological Methods, 243(1), 77.
85. Dittrich, P., & Schwille, P. (2003). An integrated microfluidic system for reaction, high-sensitivity detection, and sorting of fluorescent cells and particles. Analytical Chemistry, 75(21), 5767.
86. Pamme, N. (2006). Magnetism and microfluidics. Lab Chip, 6(1), 24.
87. Chen, C., Cho, S., Tsai, F., Erten, A., Lo, Y. (2009). Microfluidic cell sorter with integrated piezoelectric actuator. Biomedical Microdevices, 11(6), 1223.
88. Cho, S., Chen, C., Tsai, F., Godin, J., Lo, Y. (2010). Human mammalian cell sorting using a highly integrated micro-fabricated fluorescence-activated cell sorter (μFACS). Lab Chip, 10(12), 1567.
89. Baret, J., Miller, O., Taly, V., Ryckelynck, M., El-Harrak, A., Frenz, L., Rick, C., Samuels, M., Hutchison, J., Agresti, J., et al. (2009). Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. Lab Chip, 9(13), 1850.
90. Franke, T., Braunmüller, S., Schmid, L., Wixforth, A., Weitz, D. (2010). Surface acoustic wave actuated cell sorting (SAWACS). Lab Chip, 10(6), 789.
91. Huang, L., Cox, E., Austin, R., Sturm, J. (2004). Continuous particle separation through deterministic lateral displacement. Science, 304(5673), 987.
92. Takagi, J., Yamada, M., Yasuda, M., Seki, M. (2005). Continuous particle separation in a microchannel having asymmetrically arranged multiple branches. Lab Chip, 5(7), 778.
93. Hsu, C., Di, D., Carlo, Chen, C., Irimia, D., Toner, M. (2008). Microvortex for focusing, guiding and sorting of particles. Lab Chip, 8(12), 2128.
94. Choi, S., Song, S., Choi, C., Park, J. (2007). Continuous blood cell separation by hydrophoretic filtration. Lab Chip, 7(11), 1532.
95. Toner, M., & Irimia, D. (2005). Blood-on-a-chip. Annual Review of Biomedical Engineering, 7, 77.
96. Vahey, M., & Voldman, J. (2008). An equilibrium method for continuous-flow cell sorting using dielectrophoresis. Analytical Chemistry, 80(9), 3135.
97. Cheng, I., Froude, V., Zhu, Y., Chang, H., Chang, H. (2009). A continuous high-throughput bioparticle sorter based on 3D traveling-wave dielectrophoresis. Lab Chip, 9(22), 3193.
98. Huang, R., Barber, T., Schmidt, M., Tompkins, R., Toner, M., Bianchi, D., Kapur, R., Flejter, W. (2008). A microfluidics approach for the isolation of nucleated red blood cells (NRBCs) from the peripheral blood of pregnant women. Prenatal Diagnosis, 28(10), 892.
99. Lau, A., Lee, L., Chan, J. (2008). An integrated optofluidic platform for Raman-activated cell sorting. Lab Chip, 8(7), 1116.
100. Pamme, N. (2007). Continuous flow separations in microfluidic devices. Lab Chip, 7(12), 1644.
101. Tsutsui, H., & Ho, C. (2009). Cell separation by non-inertial force fields in microfluidic systems. Mechanics Research Communications, 36(1), 92.
102. Lenshof, A., & Laurell, T. (2010). Continuous separation of cells and particles in microfluidic systems. Chemical Society Reviews, 39(3), 1203.
103. Gossett, D., Weaver, W., Mach, A., Hur, S., Tse, H., Lee, W., Amini, H., Di Carlo, D. (2010). Label-free cell separation and sorting in microfluidic systems. Analytical and Bioanalytical Chemistry, 397(8), 3249.
104. Bonnet, D., & Dick, J. (1997). Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. Nature Medicine, 3(7), 730.
105. Lapidot, T., Sirard, C., Vormoor, J., Murdoch, B., Hoang, T., Caceres-Cortes, J., Minden, M., Paterson, B., Caligiuri, M., Dick, J. (1994). A cell initiating human acute myeloid leukaemia after transplantation into SCID mice. Nature, 367(6464), 645.
106. Al-Hajj, M., Wicha, M., Benito-Hernandez, A., Morrison, S., Clarke, M. (2003). Prospective identification of tumorigenic breast cancer cells. Proceedings of the National Academy of Sciences, 100(7), 3983.
107. Singh, S., Hawkins, C., Clarke, I., Squire, J., Bayani, J., Hide, T., Henkelman, R., Cusimano, M., Dirks, P. (2004). Identification of human brain tumour initiating cells. Nature, 432(7015), 396.
108. Li, C., Heidt, D., Dalerba, P., Burant, C., Zhang, L., Adsay, V., Wicha, M., Clarke, M., Simeone, D. (2007). Identification of pancreatic cancer stem cells. Cancer Research, 67(3), 1030.
109. Balic, M., Lin, H., Young, L., Hawes, D., Giuliano, A., McNamara, G., Datar, R., Cote, R. (2006). Most early disseminated cancer cells detected in bone marrow of breast cancer patients have a putative breast cancer stem cell phenotype. Clinical Cancer Research, 12(19), 5615.
110. Aktas, B., Tewes, M., Fehm, T., Hauch, S., Kimmig, R., Kasimir-Bauer, S., et al. (2009). Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic breast cancer patients. Breast Cancer Research, 11(4), R46.
111. Nagrath, S., Sequist, L., Maheswaran, S., Bell, D., Irimia, D., Ulkus, L., Smith, M., Kwak, E., Digumarthy, S., Muzikansky, A., et al. (2007). Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature, 450(7173), 1235.
112. Meng, S., Tripathy, D., Frenkel, E., Shete, S., Naftalis, E., Huth, J., Beitsch, P., Leitch, M., Hoover, S., Euhus, D., et al. (2004). Circulating tumor cells in patients with breast cancer dormancy. Clinical Cancer Research, 10(24), 8152.
113. Lo utherback, K., D'Silva, J., Liu, L., Wu, A., Austin, R., Sturm, J. (2012). Deterministic separation of cancer cells from blood at 10 mL/min. Available from Nature Precedings. doi: 10101/npre. 2012. 6861. 1.