Advances in genetic engineering of marine algae

Biotechnology Advances

Volumn 30, Issue 6, 2012, Pages 1602-1613

  Qin, Song ^a   Lin, Hanzhi ^b,c   Jiang, Peng ^b  

^a YANTAI INSTITUTE OF COASTAL ZONE RESEARCH   (China)

^b INSTITUTE OF OCEANOLOGY   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Biosafety; Expression system; Genetic engineering; Genomics; Marine algae; Photobioreactor; Transgenic algae; Vector

Indexed keywords

BIOSAFETY; EXPRESSION SYSTEM; GENOMICS; MARINE ALGAE; PHOTOBIORE-ACTOR; TRANSGENICS;

CHEMICAL INDUSTRY; GENETIC ENGINEERING; INDUSTRIAL APPLICATIONS; PROFITABILITY; VECTORS;

ALGAE;

SEA WATER;

ALGA; BAIT; BIOMASS; BIOREACTOR; BIOSAFETY; GENETIC ENGINEERING; GENETICALLY MODIFIED ORGANISM; GENOMICS; INDUSTRIAL PRODUCTION; MARICULTURE; MARINE TECHNOLOGY;

AQUATIC SPECIES; EUKARYOTE; GENETIC ENGINEERING; GENETIC TRANSFORMATION; GENETICS; GENOMICS; HUMAN; METHODOLOGY; PHOTOBIOREACTOR; REVIEW;

AQUATIC ORGANISMS; EUKARYOTA; GENETIC ENGINEERING; GENOMICS; HUMANS; PHOTOBIOREACTORS; SEAWATER; TRANSFORMATION, GENETIC;

ALGAE; ANIMALIA;

EID: 84867696055     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biotechadv.2012.05.004     Document Type: Review

References (188)

1. Agapakis C.M., Niederholtmeyer H., Noche R.R., Lieberman T.D., Megason S.G., Way J.C. Towards a synthetic chloroplast. PLoS One 2011, 6.
2. Allen J.F., de Paula W.B.M., Puthiyaveetil S., Nield J. A structural phylogenetic map for chloroplast photosynthesis. Trends Plant Sci 2011, 16:645-655.
3. Angaji S.A., Hedayati S.S., Poor R.H., Poor S.S., Shiravi S., Madani S. Application of RNA interference in plants. Plant Omics 2010, 3:77-84.
4. Anila N., Chandrashekar A., Ravishankar G.A., Sarada R. Establishment of Agrobacterium tumefaciens-mediated genetic transformation in Dunaliella bardawil. Eur J Phycol 2011, 46:36-44.
5. Apt K.E., Behrens P.W. Commercial developments in microalgal biotechnology. J Phycol 1999, 35:215-226.
6. Apt K.E., KrothPancic P.G., Grossman A.R. Stable nuclear transformation of the diatom Phaeodactylum tricornutum. Mol Gen Genet 1996, 252:572-579.
7. Archibald J.M., Rogers M.B., Toop M., Ishida K., Keeling P.J. Lateral gene transfer and the evolution of plastid-targeted proteins in the secondary plastid-containing alga Bigelowiella natans. Proc Natl Acad Sci U S A 2003, 100:7678-7683.
8. Armbrust E.V., Berges J.A., Bowler C., Green B.R., Martinez D., Putnam N.H., et al. The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. Science 2004, 306:79-86.
9. Ashby M.K., Houmard J. Cyanobacterial two-component proteins: structure, diversity, distribution, and evolution. Microbiol Mol Biol Rev 2006, 70. 472-+.
10. Baulcombe D. RNA silencing in plants. Nature 2004, 431:356-363.
11. Baweja P., Sahoo D., Garcia-Jimenez P., Robaina R.R. Review: seaweed tissue culture as applied to biotechnology: problems, achievements and prospects. Phycol Res 2009, 57:45-58.
12. Berry J.P., Gantar M., Perez M.H., Berry G., Noriega F.G. Cyanobacterial toxins as allelochemicals with potential applications as algaecides, herbicides and insecticides. Mar Drugs 2008, 6:117-146.
13. Brahamsha B. A genetic manipulation system for oceanic cyanobacteria of the genus Synechococcus. Appl Environ Microbiol 1996, 62:1747-1751.
14. Brown L.E., Sprecher S.L., Keller L.R. Introduction of Exogenous DNA into Chlamydomonas-Reinhardtii by Electroporation. Mol Cell Biol 1991, 11:2328-2332.
15. Buzby J.S., Porter R.D., Stevens S.E. Expression of the Escherichia coli lacZ gene on a plasmid vector in a cyanobacterium. Science 1985, 230:805-807.
16. Cerutti H., Ma X.R., Msanne J., Repas T. RNA-mediated silencing in algae: biological roles and tools for analysis of gene function. Eukaryot Cell 2011, 10:1164-1172.
17. Cha T.S., Chen C.F., Yee W., Aziz A., Loh S.H. Cinnamic acid, coumarin and vanillin: alternative phenolic compounds for efficient Agrobacterium-mediated transformation of the unicellular green alga, Nannochloropsis sp. J Microbiol Methods 2011, 84:430-434.
18. Chan C.X., Reyes-Prieto A., Bhattacharya D. Red and green algal origin of diatom membrane transporters: insights into environmental adaptation and Cell evolution. PLoS One 2011, 6.
19. Cheney D., Kurtzman Progress in protoplast fusion and gene transfer in red algae. Proceedings of the XIV International Seaweed Symposium. Brittany, France 1992, 68.
20. Cheney D., Metz B., Stiller J. Agrobacterium-mediated genetic transformation in the macroscopic marine red alga Porphyra yezoensis. J Phycol 2001, 37:11.
21. Chisti Y. Biodiesel from microalgae. Biotechnol Adv 2007, 25:294-306.
22. Cock J.M., Sterck L., Rouze P., Scornet D., Allen A.E., Amoutzias G., et al. The Ectocarpus genome and the independent evolution of multicellularity in brown algae. Nature 2010, 465:617-621.
23. Coll J.M. Methodologies for transferring DNA into eukaryotic microalgae. Span J Agric Res 2006, 4:316-330.
24. Cotsaftis O., Sallaud C., Breitler J.C., Meynard D., Greco R., Pereira A., et al. Transposon-mediated generation of T-DNA- and marker-free rice plants expressing a Bt endotoxin gene. Mol Breed 2002, 10:165-180.
25. Darbani B., Eimanifar A., Stewart C.N., Camargo W.N. Methods to produce marker-free transgenic plants. Biotechnol J 2007, 2:83-90.
26. De Riso V., Raniello R., Maumus F., Rogato A., Bowler C., Falciatore A. Gene silencing in the marine diatom Phaeodactylum tricornutum. Nucleic Acids Res 2009, 37.
27. DeGray G., Rajasekaran K., Smith F., Sanford J., Daniell H. Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi. Plant Physiol 2001, 127:852-862.
28. Delaroque N., Muller D.G., Bothe G., Pohl T., Knippers R., Boland W. The complete DNA sequence of the Ectocarpus siliculosus virus EsV-1 genome. Virology 2001, 287:112-132.
29. Deng X., Zhang Q., Jiang P., Cui Y., Qin S. The optimization for cell cultivation of transgenic Laminaria japonica gametophytes in a bubble-column bioreactor. J Biotechnol 2008, 136:559-560.
30. Deng X.Y., Qin S., Zhang Y.C., Jiang P. Comparison of photobioreactors and optimal light regime for rapid vegetative propagation of transgenic Undaria pinnatifida gametophytes. J Chem Technol Biotechnol 2009, 84:1486-1492.
31. Depicker A., VanMontagu M. Post-transcriptional gene silencing in plants. Curr Opin Cell Biol 1997, 9:373-382.
32. Derelle E., Ferraz C., Rombauts S., Rouze P., Worden A.Z., Robbens S., et al. Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features. Proc Natl Acad Sci U S A 2006, 103:11647-11652.
33. Douglas S., Zauner S., Fraunholz M., Beaton M., Penny S., Deng L.T., et al. The highly reduced genome of an enslaved algal nucleus. Nature 2001, 410:1091-1096.
34. Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., et al. Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome. Proc Natl Acad Sci U S A 2003, 100:10020-10025.
35. Dunahay T.G. Transformation of Chlamydomonas-Reinhardtii with Silicon-Carbide Whiskers. Biotechniques 1993, 15. 452-&.
36. Dunahay T.G., Jarvis E.E., Roessler P.G. Genetic transformation of the diatoms Cyclotella cryptica and Navicula saprophila. J Phycol 1995, 31:1004-1012.
37. El Alaoui S., Diez J., Humanes L., Toribio F., Partensky F., Garcia-Fernandez J.M. In vivo regulation of glutamine synthetase activity in the marine chlorophyll b-containing cyanobacterium Prochlorococcus sp strain PCC 9511 (oxyphotobacteria). Appl Environ Microbiol 2001, 67:2202-2207.
38. Falciatore A., Casotti R., Leblanc C., Abrescia C., Bowler C. Transformation of nonselectable reporter genes in marine diatoms. Mar Biotechnol 1999, 1:239-251.
39. Falkowski P.G., Barber R.T., Smetacek V. Biogeochemical controls and feedbacks on ocean primary production. Science 1998, 281:200-206.
40. Farrokhi N., Whitelegge J.P., Brusslan J.A. Plant peptides and peptidomics. Plant Biotechnol J 2008, 6:105-134.
41. Feng S.Y., Xue L.X., Liu H.T., Lu P.J. Improvement of efficiency of genetic transformation for Dunaliella salina by glass beads method. Mol Biol Rep 2009, 36:1433-1439.
42. Fischer H., Robl I., Sumper M., Kroger N. Targeting and covalent modification of cell wall and membrane proteins heterologously expressed in the diatom Cylindrotheca fusiformis (Bacillariophyceae). J Phycol 1999, 35:113-120.
43. Franklin S.E., Mayfield S.P. Prospects for molecular farming in the green alga Chlamydomonas reinhardtii. Curr Opin Plant Biol 2004, 7:159-165.
44. Franklin S.E., Mayfield S.P. Recent developments in the production of human therapeutic proteins in eukaryotic algae. Expert Opin Biol Ther 2005, 5:225-235.
45. Garcia-Gonzalez M., Moreno J., Canavate J.P., Anguis V., Prieto A., Manzano C., et al. Conditions for open-air outdoor culture of Dunaliella salina in southern Spain. J Appl Phycol 2003, 15:177-184.
46. Garcia-Gonzalez M., Moreno J., Manzano J.C., Florencio F.J., Guerrero M.G. Production of Dunaliella salina biomass rich in 9-cis-beta-carotene and lutein in a closed tubular photobioreactor. J Biotechnol 2005, 115:81-90.
47. Gelvin S.B. Agrobacterium and plant genes involved in T-DNA transfer and integration. Annu Rev Plant Phys 2000, 51:223-256.
48. Gilson P.R., Su V., Slamovits C.H., Reith M.E., Keeling P.J., McFadden G.I. Complete nucleotide sequence of the chlorarachniophyte nucleomorph: nature's smallest nucleus. Proc Natl Acad Sci U S A 2006, 103:9566-9571.
49. Gray M.W. The Endosymbiont Hypothesis Revisited. Int Rev Cytol 1992, 141:233-357.
50. Green B.R. Chloroplast genomes of photosynthetic eukaryotes. Plant J 2011, 66:34-44.
51. Griesbeck C., Kobl I., Heitzer M. Chlamydomonas reinhardtii. Mol Biotechnol 2006, 34:213-223.
52. Grobbelaar J.U. Factors governing algal growth in photobioreactors: the "open" versus "closed" debate. J Appl Phycol 2009, 21:489-492.
53. Grobbelaar J.U., Nedbal L., Tichy L., Setlik I. Variation in some photosynthetic characteristics of microalgae cultured in outdoor thin-layered sloping reactors. J Appl Phycol 1995, 7:175-184.
54. Grossman A.R. Chlamydomonas reinhardtii and photosynthesis: genetics to genomics. Curr Opin Plant Biol 2000, 3:132-137.
55. Grzebyk D., Schofield O., Vetriani C., Falkowski P.G. The mesozoic radiation of eukaryotic algae: the portable plastid hypothesis. J Phycol 2003, 39:259-267.
56. Gustafsson C., Govindarajan S., Minshull J. Codon bias and heterologous protein expression. Trends Biotechnol 2004, 22:346-353.
57. Gutu A., Kehoe D.M. Emerging Perspectives on the Mechanisms, Regulation, and Distribution of Light Color Acclimation in Cyanobacteria. Mol Plant 2012, 5:1-13.
58. Haapa S., Taira S., Heikkinen E., Savilahti H. An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications. Nucleic Acids Res 1999, 27:2777-2784.
59. Hamilton C.M., Frary A., Lewis C., Tanksley S.D. Stable transfer of intact high molecular weight DNA into plant chromosomes. Proc Natl Acad Sci U S A 1996, 93:9975-9979.
60. Hannon M., Gimpel J., Tran M., Rasala B., Mayfield S. Biofuels from algae: challenges and potential. Biofuels 2010, 1:763-784.
61. Hansen F.C., Reckermann M., Breteler W., Riegman R. Phaeocystis blooming enhanced by copepod predation on protozoa - evidence from incubation experiments. Mar Ecol Prog Ser 1993, 102:51-57.
62. Heidorn T., Camsund D., Huang H.H., Lindberg P., Oliveira P., Stensjo K. Synthetic biology in cyanobacteria : engineering and analyzing novel functions. Vol 497: Synthetic Biology, Methods for Part/Device Characterization and Chassis Engineering, Pt A 2011, 539-579. Elsevier Academic Press Inc., San Diego. C. Voigt (Ed.).
63. Heitzer M., Eckert A., Fuhrmann M., Griesbeck C. Influence of codon bias on the expression of foreign genes in microalgae. Adv Exp Med Biol 2007, 616:46-53.
64. Henry E.C., Meints R.H. Recombinant Viruses as Transformation Vectors of Marine Macroalgae. J Appl Phycol 1994, 6:247-253.
65. Hirakawa Y., Kofuji R., Ishida K. Transient transformation of a chlorarachniophyte alga, Lotharella amoebiformis (chlorarachniophyceae), with uidA and egfp reporter genes. J Phycol 2008, 44:814-820.
66. Hirata R., Takahashi M., Saga N., Mikami K. Transient Gene Expression System Established in Porphyra yezoensis Is Widely Applicable in Bangiophycean Algae. Mar Biotechnol 2011, 13:1038-1047.
67. Huang X., Weber J.C., Hinson T.K., Mathieson A.C., Minocha S.C. Transient expression of the GUS reporter gene in the protoplasts and partially digested cells of Ulva lactuca L (Chlorophyta). Bot Marina 1996, 39:467-474.
68. Jan P.S., Huang H.Y., Chen H.M. Expression of a Synthesized Gene Encoding Cationic Peptide Cecropin B in Transgenic Tomato Plants Protects against Bacterial Diseases. Appl Environ Microbiol 2010, 76:769-775.
69. Jia Y.L., Xue L.X., Liu H.T., Li J. Characterization of the Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Gene from the Halotolerant Alga Dunaliella salina and Inhibition of Its Expression by RNAi. Curr Microbiol 2009, 58:426-431.
70. Jiang P., Qin S., Tseng C.K. Expression of the lacZ reporter gene in sporophytes of the seaweed Laminaria japonica (Phaeophyceae) by gametophyte-targeted transformation. Plant Cell Rep 2003, 21:1211-1216.
71. John R.P., Anisha G.S., Nampoothiri K.M., Pandey A. Micro and macroalgal biomass: a renewable source for bioethanol. Bioresour Technol 2011, 102:186-193.
72. Kawata Y., Yano S., Kojima H., Toyomizu M. Transformation of Spirulina platensis strain C1 (Arthrospira sp PCC9438) with Tn5 transposase-transposon DNA-cation liposome complex. Mar Biotechnol 2004, 6:355-363.
73. Keeling P.J., Palmer J.D. Horizontal gene transfer in eukaryotic evolution. Nat Rev Genet 2008, 9:605-618.
74. Kehoe D.M., Gutu A. Responding to color: the regulation of complementary chromatic adaptation. Annu Rev Plant Biol 2006, 57:127-150.
75. Kikuchi A., Watanabe K., Tanaka Y., Kamada H. Recent progress on environmental biosafety assessment of genetically modified trees and floricultural plants in Japan. Plant Biotechnol 2008, 25:9-15.
76. Kilian O., Benemann C.S.E., Niyogi K.K., Vick B. High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp. Proc Natl Acad Sci U S A 2011, 108:21265-21269.
77. Kindle K.L. High-Frequency Nuclear Transformation of Chlamydomonas-Reinhardtii. Proc Natl Acad Sci U S A 1990, 87:1228-1232.
78. Kopertekh L., Juttner G., Schiemann J. PVX-Cre-mediated marker gene elimination from transgenic plants. Plant Mol Biol 2004, 55:491-500.
79. Koski M., Dutz J., Breteler W. Selective grazing of Temora longicornis in different stages of a Phaeocystis globosa bloom - a mesocosm study. Harmful Algae 2005, 4:915-927.
80. Kuang M., Wang S.-j., Li Y., Shen D.-l., Zeng C.-k. Transient expression of exogenous gus gene in Porphyra yezoensis (Rhodophyta). Chin J Oceanol Limnol 1998, 16:56-61.
81. Kumar S.V., Misquitta R.W., Reddy V.S., Rao B.J., Rajam M.V. Genetic transformation of the green alga - Chlamydomonas reinhardtii by Agrobacterium tumefaciens. Plant Sci 2004, 166:731-738.
82. Kurtzman A.L., Cheney D.P. Direct gene transfer and transient gene expression in a marine red alga using the biolistic method. J Phycol 1991, 27:42.
83. Langridge P., Brown J.W.S., Pintor-Toro J.A., Feix G., Neuhaus G., Neuhaus-Url G. Expression of zein genes in Acetabulara mditerranea. Eur J Cell Biol 1985, 257-264.
84. Lee L.Y., Gelvin S.B. T-DNA binary vectors and systems. Plant Physiol 2008, 146:325-332.
85. Lee S.B., Li B.C., Jin S.X., Daniell H. Expression and characterization of antimicrobial peptides Retrocyclin-101 and Protegrin-1 in chloroplasts to control viral and bacterial infections. Plant Biotechnol J 2011, 9:100-115.
86. Leon R., Fernandez E. Nuclear transformation of eukaryotic microalgae - Historical overview, achievements and problems. Adv Exp Med Biol 2007, 616:1-11.
87. León-Bañares R., Gonzalez-Ballester D., Galvan A., Fernandez E. Transgenic microalgae as green cell-factories. Trends Biotechnol 2004, 22:45-52.
88. Lerche K., Hallmann A. Stable nuclear transformation of Gonium pectorale. BMC Biotechnol 2009, 9.
89. Li F.C., Qin S., Jiang P., Wu Y., Zhang W. The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta). J Appl Phycol 2009, 21:287-293.
90. Li J., Lu Y.M., Xue L.X., Xie H. A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina. Mol Biol Rep 2010, 37:1143-1154.
91. Liang C.W., Zhang X.W., Zou J., Xu D., Su F., Ye N.H. Identification of miRNA from Porphyra yezoensis by High-Throughput Sequencing and Bioinformatics Analysis. PLoS One 2010, 5.
92. Lin H., Qin S., Jiang P. Biotechnology of seaweeds: facing the coming decade. Handbook of Marine Macroalgae 2011, 424-430. John Wiley & Sons, Ltd.
93. Liu H.Q., Yu W.G., Dai J.X., Gong Q.H., Yang K.F., Zhang Y.P. Increasing the transient expression of GUS gene in Porphyra yezoensis by 18S rDNA targeted homologous recombination. J Appl Phycol 2003, 15:371-377.
94. Lowe B.A., Prakash N.S., Way M., Mann M.T., Spencer T.M., Boddupalli R.S. Enhanced single copy integration events in corn via particle bombardment using low quantities of DNA. Transgenic Res 2009, 18:831-840.
95. Lu Y.M., Li J., Xue L.X., Yan H.X., Yuan H.J., Wang C. A duplicated carbonic anhydrase 1 (DCA1) promoter mediates the nitrate reductase gene switch of Dunaliella salina. J Appl Phycol 2011, 23:673-680.
96. Maliga P., Maliga Z.S. Biosafety evaluation of plastid transgenes in tobacco. Plant Biol (Rockville) 2006, 2006:315.
97. Manimaran P., Ramkumar G., Sakthivel K., Sundaram R.M., Madhav M.S., Balachandran S.M. Suitability of non-lethal marker and marker-free systems for development of transgenic crop plants: present status and future prospects. Biotechnol Adv 2011, 29:703-714.
98. Marenkova T.V., Deineko E.V. Transcriptional Gene Silencing in Plants. Russ J Genet 2010, 46:511-520.
99. Mary I., Vaulot D. Two-component systems in Prochlorococcus MED4: genomic analysis and differential expression under stress. FEMS Microbiol Lett 2003, 226:135-144.
100. Matsunaga T., Takeyama H. Genetic-Engineering in Marine Cyanobacteria. J Appl Phycol 1995, 7:77-84.
101. Matsunaga T., Takeyama H., Nakamura N. Characterization of Cryptic Plasmids from Marine Cyanobacteria and Construction of a Hybrid Plasmid Potentially Capable of Transformation of Marine Cyanobacterium, Synechococcus Sp and Its Transformation. Appl Biochem Biotechnol 1990, 24-5:151-160.
102. Matsunaga T., Sakaguchi T., Tadokoro F. Magnetite Formation by a Magnetic Bacterium Capable of Growing Aerobically. Appl Microbiol Biotechnol 1991, 35:651-655.
103. McArthur G.H., Fong S.S. Toward Engineering Synthetic Microbial Metabolism. J Biomed Biotechnol 2010, Article ID 459760, 10 pages. http://dx.doi.org/10.1155/2010/459760.
104. Meyers B., Zaltsman A., Lacroix B., Kozlovsky S.V., Krichevsky A. Nuclear and plastid genetic engineering of plants: comparison of opportunities and challenges. Biotechnol Adv 2010, 28:747-756.
105. Miki B., McHugh S. Selectable marker genes in transgenic plants: applications, alternatives and biosafety. J Biotechnol 2004, 107:193-232.
106. Miyagawa A., Okami T., Kira N., Yamaguchi H., Ohnishi K., Adachi M. Research note: high efficiency transformation of the diatom Phaeodactylum tricornutum with a promoter from the diatom Cylindrotheca fusiformis. Phycol Res 2009, 57:142-146.
107. Miyagawa-Yamaguchi A., Okami T., Kira N., Yamaguchi H., Ohnishi K., Adachi M. Stable nuclear transformation of the diatom Chaetoceros sp. Phycol Res 2011, 59:113-119.
108. Moheimani N.R., Borowitzka M.A. The long-term culture of the coccolithophore Pleurochrysis carterae (Haptophyta) in outdoor raceway ponds. J Appl Phycol 2006, 18:703-712.
109. Moheimani N.R., Isdepsky A., Lisec J., Raes E., Borowitzka M.A. Coccolithophorid Algae Culture in Closed Photobioreactors. Biotechnol Bioeng 2011, 108:2078-2087.
110. Montsant A., Allen A.E., Coesel S., De Martino A., Falciatore A., Mangogna M., et al. Identification and comparative genomic analysis of signaling and regulatory components in the diatom Thalassiosira pseudonana. J Phycol 2007, 43:585-604.
111. Muers M. SYNTHETIC BIOLOGY Simplifying design. Nat Rev Genet 2012, 13:72.
112. Neuhaus G., Spangenberg G. Plant Transformation by Microinjection Techniques. Physiol Plant 1990, 79:213-217.
113. Neuhaus G., Neuhausurl G., Degroot E.J., Schweiger H.G. High-yield and stable transformation of the unicellular green-alga Acetabularia by microinjection of SV40 and PSV2NEO. EMBO J 1986, 5:1437-1444.
114. Neumann E., Schaeferridder M., Wang Y., Hofschneider P.H. Gene-Transfer into Mouse Lyoma Cells by Electroporation in High Electric-Fields. EMBO J 1982, 1:841-845.
115. Oh S.-D., Shin H., Sohn S.-I., Lee K., Kim H.-J., Ryu T.-H., et al. Evaluation and assessment of biosafety for Bacillus thuringiensis (Bt)-transgenic rice: responses of Daphnia magna Fed on Bt-transgenic rice variety. J Appl Biol Chem 2011, 54:296-302.
116. Opabode J.T. Agrobacterium-mediated transformation of plants: emerging factors that influence efficiency. Biotechnol Mol Biol Rev 2006, 1:12-20.
117. Ow D.W. Recombinase-directed plant transformation for the post-genomic era. Plant Mol Biol 2002, 48:183-200.
118. Pan K., Qin J.J., Li S., Dai W.K., Zhu B.H., Jin Y.C., et al. Nuclear Monoploidy and Asexual Propagation of Nannochloropsis Oceanica (Eustigmatophyceae) as Revealed by Its Genome Sequence. J Phycol 2011, 47:1425-1432.
119. Parker M.S., Mock T., Armbrust E.V. Genomic Insights into Marine Microalgae. Annu Rev Genet 2008, 42:619-645.
120. Paszkowski J., Whitham S.A. Gene silencing and DNA methylation processes. Curr Opin Plant Biol 2001, 4:123-129.
121. Pfeiffer A., Kunkel T., Hiltbrunner A., Neuhaus G., Wolf I., Speth V., et al. A cell-free system for light-dependent nuclear import of phytochrome. Plant J 2009, 57:680-689.
122. Polnefuller M., Gibor A. Developmental studies in Porphyra. 1. Blade differentiation in Porphyra perforata as expressed by morphology, enzymatic digestion, and protoplast regeneration. J Phycol 1984, 20:609-616.
123. Porter R.D. Transformation in Cyanobacteria. CRC Crit Rev Microbiol 1986, 13:111-132.
124. Potvin G., Zhang Z.S. Strategies for high-level recombinant protein expression in transgenic microalgae: a review. Biotechnol Adv 2010, 28:910-918.
125. Poulsen N., Kröger N. A new molecular tool for transgenic diatoms - Control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette. FEBS J 2005, 272:3413-3423.
126. Poulsen N., Chesley P.M., Kroger N. Molecular genetic manipulation of the diatom Thalassiosira pseudonana (Bacillariophyceae). J Phycol 2006, 42:1059-1065.
127. Price D.C., Chan C.X., Yoon H.S., Yang E.C., Qiu H., Weber A.P.M., et al. Cyanophora paradoxa Genome Elucidates Origin of Photosynthesis in Algae and Plants. Science 2012, 335:843-847.
128. Pulz O., Gross W. Valuable products from biotechnology of microalgae. Appl Microbiol Biotechnol 2004, 65:635-648.
129. Purnick P.E.M., Weiss R. The second wave of synthetic biology: from modules to systems. Nat Rev Mol Cell Biol 2009, 10:410-422.
130. Qin S., Zhang J., Li W.B., Wang X.H., Tong S., Sun Y.R., et al. Transient expression of GUS gene in Phaeophytes using Biolistic Particle Delivery System. Oceanol Limnol Sin 1994, 25:353-356.
131. Qin S., Jiang P., Li X.P., Wang X.H., Zeng C.K. A transformation model for Laminaria japonica (Phaeophyta, Laminariales). Chin J Oceanol Limnol 1998, 16:50-55.
132. Qin S., Sun G.Q., Jiang P., Zou L.H., Wu Y., Tseng C.K. Review of genetic engineering of Laminaria japonica (Laminariales, Phaeophyta) in China. Hydrobiologia 1999, 399:469-472.
133. Qin S., Jiang P., Tseng C.K. Molecular biotechnology of marine algae in China. Hydrobiologia 2004, 512:21-26.
134. Qin S., Jiang P., Tseng C. Transforming kelp into a marine bioreactor. Trends Biotechnol 2005, 23:264-268.
135. Rahnamaeian M., Vilcinskas A. Defense gene expression is potentiated in transgenic barley expressing antifungal peptide metchnikowin throughout powdery mildew challenge. J Plant Res 2012, 125:115-124.
136. Ramessar K., Peremarti A., Gomez-Galera S., Naqvi S., Moralejo M., Munoz P., et al. Biosafety and risk assessment framework for selectable marker genes in transgenic crop plants: a case of the science not supporting the politics. Transgenic Res 2007, 16:261-280.
137. Rastogi R.P., Sinha R.P. Biotechnological and industrial significance of cyanobacterial secondary metabolites. Biotechnol Adv 2009, 27:521-539.
138. Reddy C., Gupta M., Mantri V., Jha B. Seaweed protoplasts: status, biotechnological perspectives and needs. J Appl Phycol 2008, 20:619-632.
139. Remacle C., Cline S., Boutaffala L., Gabilly S., Larosa V., Barbieri M.R., et al. The ARG9 Gene Encodes the Plastid-Resident N-Acetyl Ornithine Aminotransferase in the Green Alga Chlamydomonas reinhardtii. Eukaryot Cell 2009, 8:1460-1463.
140. Reyes-Prieto A., Bhattacharya D. Phylogeny of nuclear-encoded plastid-targeted proteins supports an early divergence of glaucophytes within plantae. Mol Biol Evol 2007, 24:2358-2361.
141. Reznikoff W.S. Transposon Tn5. Annu Rev Genet 2008, 42:269-286.
142. Richmond A., Cheng-Wu Z. Optimization of a flat plate glass reactor for mass production of Nannochloropsis sp outdoors. J Biotechnol 2001, 85:259-269.
143. Rocap G., Larimer F.W., Lamerdin J., Malfatti S., Chain P., Ahlgren N.A., et al. Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation. Nature 2003, 424:1042-1047.
144. Rorrer G.L., Cheney D.P. Bioprocess engineering of cell and tissue cultures for marine seaweeds. Aquacult Eng 2004, 32:11-41.
145. Rorrer G.L., Mullikin R.K., Huang B., Gerwick W.H., Maliakal S., CD P. Production of bioactive metabolites by cell and tissue cultures of marine macroalgae in bioreactor systems. Plant Cell and Tissue Culture for the Production of Food Ingredients 1998, 165-184. Kluwer Academic/Plenum Publishing, New York. T.-J. Fu, G. Singh, W.R. Curtis (Eds.).
146. Sakaguchi T., Nakajima K., Matsuda Y. Identification of the UMP Synthase Gene by Establishment of Uracil Auxotrophic Mutants and the Phenotypic Complementation System in the Marine Diatom Phaeodactylum tricornutum. Plant Physiol 2011, 156:78-89.
147. Scanlan D.J., Ostrowski M., Mazard S., Dufresne A., Garczarek L., Hess W.R. Ecological Genomics of Marine Picocyanobacteria. Microbiol Mol Biol Rev 2009, 73. 249-+.
148. Schmidt B.J., Lin-Schmidt X., Chamberlin A., Salehi-Ashtiani K., Papin J.A. Metabolic systems analysis to advance algal biotechnology. Biotechnol J 2010, 5:660-670.
149. Schnorf M., Neuhaus-Url G., Galli A., Iida S., Potrykus I., Neuhaus G. An improved approach for transformation of plant cells by microinjection: molecular and genetic analysis. Transgenic Res 1991, 1:23-30.
150. Schramke V., Allshire R. Retraction - Hairpin RNAs and retrotransposon LTRs effect RNAi and chromatin-based gene silencing (vol 301, pg 1069, 2003). Science 2005, 49.
151. Shimogawara K., Fujiwara S., Grossman A., Usuda H. High-efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics 1998, 148:1821-1828.
152. Sode K., Tatara M., Takeyama H., Burgess J.G., Matsunaga T. Conjugative gene-transfer in marine cyanobacteria - Synechococcus sp., Synechocystis sp. and Pseudanabeaena sp. Appl Microbiol Biotechnol 1992, 37:369-373.
153. Sripriya R., Raghupathy V., Veluthambi K. Generation of selectable marker-free sheath blight resistant transgenic rice plants by efficient co-transformation of a cointegrate vector T-DNA and a binary vector T-DNA in one Agrobacterium tumefaciens strain. Plant Cell Rep 2008, 27:1635-1644.
154. Steinbrenner J., Sandmann G. Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis. Appl Environ Microbiol 2006, 72:7477-7484.
155. Stevens S.E., Porter R.D. Transformation in Agmenellum quadruplicatum. Proc Natl Acad Sci U S A-Biol Sci 1980, 77:6052-6056.
156. Sullivan M.B., Lindell D., Lee J.A., Thompson L.R., Bielawski J.P., Chisholm S.W. Prevalence and evolution of core photosystem II genes in marine cyanobacterial viruses and their hosts. PLoS Biol 2006, 4:1344-1357.
157. Sun G.H., Zhang X.C., Sui Z.H., Mao Y.X. Inhibition of pds gene expression via the RNA interference approach in Dunaliella salina (Chlorophyta). Mar Biotechnol 2008, 10:219-226.
158. Surzycki R., Greenham K., Kitayama K., Dibal F., Wagner R., Rochaix J.D., et al. Factors effecting expression of vaccines in microalgae. Biologicals 2009, 37:133-138.
159. Takahashi M., Uji T., Saga N., Mikami K. Isolation and regeneration of transiently transformed protoplasts from gametophytic blades of the marine red alga Porphyra yezoensis. Electron J Biotechnol 2010, 13.
160. Tan C.P., Qin S., Zhang Q., Jiang P., Zhao F.Q. Establishment of a micro-particle bombardment transformation system for Dunaliella saliva. J Microbiol 2005, 43:361-365.
161. Tanifuji G., Onodera N.T., Wheeler T.J., Dlutek M., Donaher N., Archibald J.M. Complete Nucleomorph Genome Sequence of the Nonphotosynthetic Alga Cryptomonas paramecium Reveals a Core Nucleomorph Gene Set. Genome Biol Evol 2011, 3:44-54.
162. ten Lohuis M.R., Miller D.J. Genetic transformation of dinoflagellates (Amphidinium and Symbiodinium): expression of GUS in microalgae using heterologous promoter constructs. Plant J 1998, 13:427-435.
163. Ting C.S., Rocap G., King J., Chisholm S.W. Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity. Microbiology-(UK) 2001, 147:3171-3182.
164. Tolonen A.C., Liszt G.B., Hess W.R. Genetic manipulation of Prochlorococcus strain MIT9313: green fluorescent protein expression from an RSF1010 plasmid and Tn5 transposition. Appl Environ Microbiol 2006, 72:7607-7613.
165. Tredici M.R. Mass production of microalgae: photobioreactors. Handbook of Microalgal Culture 2007, 178-214. Blackwell Publishing Ltd.
166. Tseng C.K. Algal biotechnology industries and research activities in China. J Appl Phycol 2001, 13:375-380.
167. Tzfira T., Citovsky V. Agrobacterium-mediated genetic transformation of plants: biology and biotechnology. Curr Opin Biotechnol 2006, 17:147-154.
168. Tzfira T., Tian G.W., Lacroix B., Vyas S., Li J.X., Leitner-Dagan Y., et al. pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants. Plant Mol Biol 2005, 57:503-516.
169. Van Etten J.L., Meints R.H. Giant viruses infecting algae. Annu Rev Microbiol 1999, 53:447-494.
170. Walker T.L., Collet C., Purton S. Algal transgenics in the genomic ERA. J Phycol 2005, 41:1077-1093.
171. Waller R.F., Slamovits C.H., Keeling P.J. Lateral gene transfer of a multigene region from cyanobacteria to dinotlagellates resulting in a novel plastid-targeted fusion protein. Mol Biol Evol 2006, 23:1437-1443.
172. Wang J.F., Jiang P., Cui Y.L., Guan X.Y., Qin S. Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation. Phycologia 2010, 49:355-360.
173. Wargacki A.J., Leonard E., Win M.N., Regitsky D.D., Santos C.N.S., Kim P.B., et al. An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae. Science 2012, 335:308-313.
174. Watanabe S., Ohnuma M., Sato J., Yoshikawa H., Tanaka K. Utility of a GFP reporter system in the red alga Cyanidioschyzon merolae. J Gen Appl Microbiol 2011, 57:69-72.
175. Waterhouse P.M., Graham H.W., Wang M.B. Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc Natl Acad Sci U S A 1998, 95:13959-13964.
176. Welch M., Villalobos A., Gustafsson C., Minshull J. You're one in a googol: optimizing genes for protein expression. J R Soc Interface 2009, 6.
177. Wise R.R. The diversity of plastid form and function: Springer, Po Box 17, 3300 Aa Dordrecht, Netherlands 2006.
178. Witvrouw M., DeClercq E. Sulfated polysaccharides extracted from sea algae as potential antiviral drugs. Gen Pharmacol 1997, 29:497-511.
179. Wu J., Du H.W., Liao X.W., Zhao Y., Li L.G., Yang L.Y. Tn5 transposase-assisted transformation of indica rice. Plant J 2011, 68:186-200.
180. Xiong J., Fischer W.M., Inoue K., Nakahara M., Bauer C.E. Molecular evidence for the early evolution of photosynthesis. Science 2000, 289:1724-1730.
181. Yao Q., Cong L., Chang J.L., Li K.X., Yang G.X., He G.Y. Low copy number gene transfer and stable expression in a commercial wheat cultivar via particle bombardment. J Exp Bot 2006, 57:3737-3746.
182. Yi Z.W., Huang H., Kuang T.Y., Sui S.F. Three-dimensional architecture of phycobilisomes from Nostoc flagelliforme revealed by single particle electron microscopy. FEBS Lett 2005, 579:3569-3573.
183. Yoon H.S., Hackett J.D., Ciniglia C., Pinto G., Bhattacharya D. A molecular timeline for the origin of photosynthetic eukaryotes. Mol Biol Evol 2004, 21:809-818.
184. Zaslavskaia L.A., Lippmeier J.C., Kroth P.G., Grossman A.R., Apt K.E. Transformation of the diatom Phaeodactylum tricornutum (Bacillariophyceae) with a variety of selectable marker and reporter genes. J Phycol 2000, 36:379-386.
185. Zaslavskaia L.A., Lippmeier J.C., Shih C., Ehrhardt D., Grossman A.R., Apt K.E. Trophic obligate conversion of an photoautotrophic organism through metabolic engineering. Science 2001, 292:2073-2075.
186. Zhang Z.D., Green B.R., Cavalier-Smith T. Single gene circles in dinoflagellate chloroplast genomes. Nature 1999, 400:155-159.
187. Zhang Z., Jiang P., Yang R., Fei X., Tang X., Qin S. Establishment of a concocelis-mediated transformation system for Porphyra by biolistic PDS-1000/He. Chin High Technol Lett 2010, 20:204-207.
188. Zimmermann U., Pilwat G., Riemann F. Preparation of erythrocyte ghosts by dielectric breakdown of the cell membrane. Biochim Biophys Acta (BBA) - Biomembr 1975, 375:209-219.