Attainment of 15-fold higher affinity of a fusarium-specific single-chain antibody by directed molecular evolution coupled to phage display

Molecular Biotechnology

Volumn 52, Issue 2, 2012, Pages 111-122

  Liu, Jin Long ^a   Hu, Zu Quan ^a   Xing, Shu ^a   Xue, Sheng ^a   Li, He Ping ^a   Zhang, Jing Bo ^a   Liao, Yu Cai ^a,b  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b National Center of Plant Gene Research Wuhan   (China)

Author keywords

Affinity maturation; DNA shuffling; Fusarium head blight; Single chain antibody; Surface plasmon resonance

Indexed keywords

AFFINITY MATURATION; COMBINATORIAL LIBRARY; CONFORMATIONAL CHANGE; DIRECTED EVOLUTION; DNA SHUFFLING; ENZYME LINKED IMMUNOSORBENT ASSAY; ERROR PRONE PCR; F. GRAMINEARUM; FUSARIUM GRAMINEARUM; FUSARIUM HEAD BLIGHTS; MOLECULAR EVOLUTION; PHAGE CLONES; PHAGE DISPLAY; SINGLE-CHAIN ANTIBODIES; SINGLE-CHAIN FV ANTIBODIES; SURFACE PLASMONS; THREE-DIMENSION;

CLONING; DIAGNOSIS; DNA; PLASMONS; POLYMERASE CHAIN REACTION; SURFACE PLASMON RESONANCE;

ANTIBODIES;

SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY AFFINITY; ARTICLE; BIOPANNING; COMBINATORIAL LIBRARY; CONTROLLED STUDY; DIRECTED MOLECULAR EVOLUTION; DNA SEQUENCE; DNA SHUFFLING; ENZYME LINKED IMMUNOSORBENT ASSAY; FUSARIUM GRAMINEARUM; FUSARIUM HEAD BLIGHT; IMMUNOBLOTTING; IMMUNOFLUORESCENCE TEST; MOLECULAR CLONING; MOLECULAR MODEL; NONHUMAN; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN FOLDING;

ANIMALS; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; BIOTECHNOLOGY; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; ENZYME-LINKED IMMUNOSORBENT ASSAY; FUSARIUM; MODELS, MOLECULAR; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION; SINGLE-CHAIN ANTIBODIES; SURFACE PLASMON RESONANCE;

FUSARIUM; GIBBERELLA ZEAE; TRITICUM AESTIVUM; ZEA MAYS;

EID: 84867405100     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-011-9478-3     Document Type: Article

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