Engineering the steroid-specificity of an anti-17β-estradiol Fab by random mutagenesis and competitive phage panning

Protein Engineering

Volumn 11, Issue 2, 1998, Pages 143-152

  Saviranta, Petri ^a   Pajunen, Maria ^a,b   Jauria, Piitu ^a   Karp, Matti ^a   Pettersson, Kim ^a   Mäntsälä, Pekka ^a   Lövgren, Timo ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b TURKU CENTRE FOR BIOTECHNOLOGY   (Finland)

Author keywords

Competitive panning; Error prone PCR; Estradiol; Mutation rate; Phage display

Indexed keywords

ESTRADIOL; IMMUNOGLOBULIN F(AB) FRAGMENT; TESTOSTERONE;

AMINO ACID SEQUENCE; ANTIBODY SPECIFICITY; ARTICLE; BINDING AFFINITY; CHEMICAL STRUCTURE; CLONE; CONTROLLED STUDY; CROSS REACTION; DNA SEQUENCE; GENE MUTATION; GENETIC ENGINEERING; MOLECULAR CLONING; MUTAGENESIS; MUTATION RATE; NONHUMAN; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; STEROID BINDING;

AMINO ACID SEQUENCE; ANTIBODY SPECIFICITY; BACTERIOPHAGES; BINDING, COMPETITIVE; CLONING, MOLECULAR; ESCHERICHIA COLI; ESTRADIOL; GENE LIBRARY; IMMUNOGLOBULIN FAB FRAGMENTS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; TESTOSTERONE;

EID: 0031917485     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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