Engineering Fusarium head blight resistance in wheat by expression of a fusion protein containing a Fusarium-specific antibody and an antifungal peptide

Molecular Plant-Microbe Interactions

Volumn 21, Issue 9, 2008, Pages 1242-1248

  Li, He Ping ^a   Zhang, Jing Bo ^a   Shi, Run Ping ^a   Huang, Tao ^a   Fischer, Rainer ^b   Liao, Yu Cai ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

F. asiaticum

Indexed keywords

ANTIFUNGAL PROTEIN, ASPERGILLUS; FUNGAL PROTEIN; FUNGUS ANTIBODY; HYBRID PROTEIN;

ANIMAL; ARTICLE; CHICKEN; FUSARIUM; GENETIC ENGINEERING; GENETICS; GROWTH, DEVELOPMENT AND AGING; IMMUNOLOGY; INNATE IMMUNITY; METHODOLOGY; MICROBIOLOGY; PHYSIOLOGY; PLANT DISEASE; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TRANSGENIC PLANT; WESTERN BLOTTING; WHEAT;

ANIMALS; ANTIBODIES, FUNGAL; BLOTTING, SOUTHERN; BLOTTING, WESTERN; CHICKENS; FUNGAL PROTEINS; FUSARIUM; GENETIC ENGINEERING; IMMUNITY, INNATE; PLANT DISEASES; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRITICUM;

ASPERGILLUS; ASPERGILLUS GIGANTEUS; FUSARIUM; FUSARIUM ASIATICUM; TRITICUM AESTIVUM;

EID: 51949095320     PISSN: 08940282     EISSN: None     Source Type: Journal    
DOI: 10.1094/MPMI-21-9-1242     Document Type: Article

References (38)

1. Anand, A., Zhou, T., Trick, H. N., Gill, B. S., and Muthukrishnan, S. 2003. Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum. J. Exp. Bot. 54:1001-1111.
2. Altpeter, F., Vasil, V., Saivastava, V., Stöger, E., and Vasil, I. K. 1996. Accelerated production of transgenic wheat (Triticum aestivum L.) plants. Plant Cell Rep. 16:12-17.
3. Bai, G.-H., and Shaner, G. 2004. Management and resistance in wheat and barley to Fusarium head blight. Annu. Rev. Phytopathol. 4:135-161.
4. Bai, Q. Y., 1997. The contamination of fungal mycotoxins in products. J. Agro-Environ. Sci. 16:40-43.
5. Bohlmann, H. 2004. The best of both worlds in plant protection. Nat. Biotechnol. 22:682-683.
6. Boonrod, K., Galetzka, D., Nagy, P. D., Conrad, U., and Krczal, G. 2004. Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance. Nat. Biotechnol. 22:856-862.
7. Bradford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem.72:248-254.
8. Buerstmayr, H., Steiner, B., Lemmens, M., and Ruckenbauer, P. 2000. Resistance to Fusarium head blight in winter wheat: Heritability and trait associations. Crop Sci. 40:1012-1018.
9. Chen, L. F., Bai, G. H., and Desjardins, A. E. 2000. Recent advances in wheat head scab research in China. National Agricultural Library Internet Publication, U. S. Department of Agriculture, Beltsville, MD, U.S.A. Published online
10. Chen, W. P., Chen, P. D., Liu, D. J., Kynast, R., Friebe, B., Velazhahan, R., Muthukrishnan, S., and Gill, B. S. 1999. Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene. Theor. Appl. Genet. 99:755-760.
11. Christensen, A. H., and Quail, P. H. 1996. Ubiquitin promoter-based vectors for high level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res. 5:213-218.
12. Dahleen, L. S., Okubara, P. A., and Blech, A. E. 2001. Transgenic approaches to combat Fusarium head blight in wheat and barley. Crop Sci. 41:628-637.
13. Di, R., and Tumer, N. E. 2005. Expression of a truncated form of ribosomal protein L3 confers resistance to pokeweed antiviral protein and the Fusarium mycotoxin deoxynivalenol. Mol. Plant-Microbe Interact. 18:762-770.
14. Gale, L. R., Chen, L. F., Hernick, C. A., Takamura, K., and Kistler, H. C. 2002. Population analysis of Fusarium graminearum from wheat fields in eastern China. Phytopathology 92:1315-1322.
15. Hiatt, E. E., Hill, N. S., and Hiatt, E. N. 2001. Monoclonal antibodies incorporated into Neotyphodium coenophialum fungal cultures: Inhibition of fungal growth and stability of antibodies. Fungal Genet. Biol. 33:107-114.
16. Jobling, S. A., Jarman, C., Teh, M.-M., Holmberg, N., Blake, C., and Verhoeyen, M. E. 2003. Immunomodulation of enzyme function in plants by single-domain antibody fragments. Nat. Biotechnol. 21:77-80.
17. Lacadena, J., Martinez del Pozo, A., Gasset, M., Patino, B., Campos-Olivas, R., Vazquez, C., Martinez-Ruiz, A., Mancheno, J. M., Onaderra, M., and Gavilanes, J. G. 1995. Characterization of the antifungal protein secreted by the mould Aspergillus giganteus. Arch. Biochem. Biophys. 324:273-281.
18. Li, B., Liu, F., Xu, R., Huang, C., Cheng, F., Liu, J., Meng, J., and Mou, J. 2000. Sumai3: Its development, genetic characteristics and applications in wheat breeding for Fusarium head blight resistance. Pages 187-193 in: Proc. Int. Symp. Wheat Improvement for Scab Resistance. Suzhou and Nanjing, China.
19. Liu, D. J. 2002. Genome analysis in wheat breeding for disease resistance. Acta Bot. Sin. 44:1096-1114.
20. Liu, S., and Anderson, J. A. 2003. Marker assisted evaluation of Fusarium head blight resistant wheat germplasm. Crop Sci. 43:760-766.
21. Mackintosh, C., Lewis, J., Radmer, L., Shin, S., Heinen, S., Smith, L., Wyckoff M, Dill-Macky, R., Evans, C., Kravchenko, S., Baldridge, G., Zeyen, R., and Muehlbauer, G. 2007. Overexpression of defense response genes in transgenic wheat enhances resistance to Fusarium head blight. Plant Cell Rep. 26:479-488.
22. Makandar, R., Essig, J. S., Schapaugh, M. A., Trick, H. N., and Shah, J. 2006. Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1. Mol. Plant-Microbe Interact. 19:123-129.
23. Nehra, N. S., Chibbar, R. N., Leung, N., Caswell, K., Mallard, C., Steinhauer, L., Baga, M., and Kartha, K. K. 1994. Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J. 5:285-297.
24. Nicholson, P., and Parry, D. W.1996. Development and use of a PCR assay to detect Rhizoctonia cerealis, the cause of sharp eyespot in wheat. Plant Pathol. 45:872-883.
25. O'Donnell, K., Ward, T. J., Geiser, D. M., Kistler, H. C., and Aoki, T. 2004. Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade. Fungal Genet. Biol. 41:600-623.
26. Okubara, P. A., Blech, A. E., McCormick, S. P., Alexander, N. J., Dill-Macky, R., and Hohn, T. M. 2002. Engineering deoxynivalenol metabolism in wheat through the expression of a fungal trichothecene acetyltransferase gene. Theor. Appl. Genet. 106:74-83.
27. Peschen, D., Li, H. P., Fischer, R., Kreuzaler, F., and Liao, Y. C. 2004. Fusion proteins comprising a Fusarium-specific antibody linked to antifungal peptides protect plants against fungal pathogens. Nat. Biotechnol. 22:732-738.
28. Qu, B., Li, H. P., Zhang, J. B., Xu, Y. B., Huang, T., Wu, A. B., Carter, J., Nicholson, P., and Liao, Y. C. 2008. Geographical distribution and genetic diversity of the Fusarium graminearum and F. asiaticum on wheat spikes throughout China. Plant Pathol. 57:15-24.
29. Sambrook, J., Fritsch, E. F., and Maniatis, T. 1996. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, U.S.A.
30. Steed, A., Chandler, E., Thomsett, M., Gosman, N., Faure, S., and Nicholson, P. 2005. Identification of type I resistance to Fusarium head blight controlled by a major gene located on chromosome 4A of Triticum macha. Theor. Appl. Genet. 111:521-529.
31. Tavladoraki, P., Benvenuto, E., Trinca, S., Demartinis, D., Cattaneo, A., and Galeffi, P. 1993. Transgenic plants expressing a functional single-chain Fv-antibody are specifically protected from virus attack. Nature 366:469-472.
32. Voss, A., Niersback, M., Hain, R., Hirsch, H. J., Liao, Y. C., Kreuzaler, F., and Fischer, R. 1995. Reduced virus infectivity in N. tabacum secreting a TMV-specific full size antibody. Mol. Breed. 1:39-50.
33. Waldron, B. L., Moreno-Sevilla, B., Anderson, J. A., Stack, R. W., and Frohberg, R. C. 1999. RFLP mapping of QTL for Fusarium head blight resistance in wheat. Crop Sci. 39:805-811.
34. Windels, C. E., 2000. Economic and social impacts of Fusarium head blight: Changing farms and rural communities in the Northern Great Plains. Phytopathology 90:17-21.
35. Wnendt, S., Ulbrich, N., and Stahl, U. 1990. Cloning and nucleotide sequence of a cDNA encoding the antifungal protein of Aspergillus giganteus and preliminary characterization of the native gene. Nucleic Acid Res. 18:3987.
36. Wu, A. B., Li, H. P., Zhao, C. S., and Liao, Y. C. 2005. Comparative pathogenicity of Fusarium graminearum from China revealed by wheat coleoptile and floret inoculations. Mycopathologia 160:75-83.
37. Zhang, J. B., Li, H. P., Dang, F. J., Qu, B., Xu, Y. B., Zhao, C. S., and Liao, Y. C. 2007. Determination of the trichothecene mycotoxin chemotypes and associated geographical distribution and phylogenetic species of the Fusarium graminearum clade from China. Mycol. Res. 111:967-975.
38. Zimmermann, S., Schillberg, S., Liao, Y. C., and Fischer, R. 1998. Intracellular expression of TMV-specific single-chain Fv fragments leads to improved virus resistance in Nicotiana tabacum. Mol. Breed. 4:369-379.