Proteomic analysis of Clostridium thermocellum core metabolism: Relative protein expression profiles and growth phase-dependent changes in protein expression

BMC Microbiology

Volumn 12, Issue , 2012, Pages

  Rydzak, Thomas ^a   McQueen, Peter D ^b   Krokhin, Oleg V ^b   Spicer, Vic ^a   Ezzati, Peyman ^b   Dwivedi, Ravi C ^b   Shamshurin, Dmitry ^b   Levin, David B ^a   Wilkins, John A ^b   Sparling, Richard ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

^b UNIVERSITY OF MANITOBA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; ALDEHYDE DEHYDROGENASE; CELLOBIOSE; FERREDOXIN NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE REDUCTASE; FERREDOXIN NITRITE REDUCTASE; PYRUVIC ACID;

ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL METABOLISM; CLOSTRIDIUM THERMOCELLUM; CONTROLLED STUDY; ENERGY CONSERVATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN METABOLISM; PROTEIN SYNTHESIS; PROTEOMICS;

BACTERIAL PROTEINS; CELLOBIOSE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLOSTRIDIUM THERMOCELLUM; CULTURE MEDIA; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GENE EXPRESSION PROFILING; PROTEOME; TANDEM MASS SPECTROMETRY;

CLOSTRIDIUM THERMOCELLUM;

EID: 84866481432     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-12-214     Document Type: Article

References (98)

1. The cellulosomes: multienzyme machines for degradation of plant cell wall polysaccharides. Bayer EA, Belaich JP, Shoham Y, Lamed R, Annu Rev Microbiol 2004 58 521 554 10.1146/annurev.micro.57.030502.091022 15487947 (Pubitemid 39551996)
2. Characterization of Clostridium thermocellum JW20. Freier D, Mothershed CP, Wiegel J, Appl Environ Microbiol 1988 54 1 204 211 16347527
3. Effect of substrate loading on hydrogen production during anaerobic fermentation by Clostridium thermocellum 27405. Islam R, Cicek N, Sparling R, Levin D, Appl Microbiol Biotechnol 2006 72 3 576 583 10.1007/s00253-006-0316-7 16685495 (Pubitemid 44273086)
4. Growth phase-dependant enzyme profile of pyruvate catabolism and end-product formation in Clostridium thermocellum ATCC 27405. Rydzak T, Levin DB, Cicek N, Sparling R, J Biotechnol 2009 140 3-4 169 175 19428711
5. Formate synthesis by Clostridium thermocellum during anaerobic fermentation. Sparling R, Islam R, Cicek N, Carere C, Chow H, Levin DB, Can J Microbiol 2006 52 7 681 688 10.1139/w06-021 16917525 (Pubitemid 44525749)
6. Consolidated bioprocessing of cellulosic biomass: an update. Lynd LR, van Zyl WH, McBride JE, Laser M, Curr Opin Biotechnol 2005 16 5 577 583 10.1016/j.copbio.2005.08.009 16154338 (Pubitemid 41393839)
7. Energy conservation in chemotrophic anaerobic bacteria. Thauer RK, Jungermann K, Decker K, Bacteriol Rev 1977 41 1 100 180 860983 (Pubitemid 8082287)
8. Hydrolysis of dilute acid pretreated mixed hardwood and purified microcrystalline cellulose by cell-free broth from Clostridium thermocellum. Lynd LR, Grethlein HE, Biotechnol Bioeng 1987 29 1 92 100 10.1002/bit.260290114 18561134 (Pubitemid 17059300)
9. Fermentation of Cellulosic Substrates in Batch and Continuous Culture by Clostridium thermocellum. Lynd LR, Grethlein HE, Wolkin RH, Appl Environ Microbiol 1989 55 12 3131 3139 16348075 (Pubitemid 20002124)
10. Microbial cellulose utilization: fundamentals and biotechnology. Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS, Microbiol Mol Biol Rev 2002 66 3 506 577 table of contents 10.1128/MMBR.66.3.506-577.2002 12209002 (Pubitemid 35005827)
11. Production of bioenergy and biochemicals from industrial and agricultural wastewater. Angenent LT, Karim K, Al-Dahhan MH, Wrenn BA, Domiguez-Espinosa R, Trends Biotechnol 2004 22 9 477 485 10.1016/j.tibtech.2004.07.001 15331229 (Pubitemid 39141162)
12. Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant. Tripathi SA, Olson DG, Argyros DA, Miller BB, Barrett TF, Murphy DM, McCool JD, Warner AK, Rajgarhia VB, Lynd LR, et al. Appl Environ Microbiol 2010 76 19 6591 6599 10.1128/AEM.01484-10 20693441
13. Electro transformation of Clostridium thermocellum. Tyurin MV, Desai SG, Lynd LR, Appl Environ Microbiol 2004 70 2 883 890 10.1128/AEM.70.2.883-890.2004 14766568 (Pubitemid 38568212)
14. Role of spontaneous current oscillations during high-efficiency electrotransformation of thermophilic anaerobes. Tyurin MV, Sullivan CR, Lynd LR, Appl Environ Microbiol 2005 71 12 8069 8076 10.1128/AEM.71.12.8069-8076.2005 16332787 (Pubitemid 41805091)
15. Make way for ethanol. Lynd LR, Cruz CH, Science 2010 330 6008 1176 21109651
16. Improvement of cellulolytic properties of Clostridium cellulolyticum by metabolic engineering. Guedon E, Desvaux M, Petitdemange H, Appl Environ Microbiol 2002 68 1 53 58 10.1128/AEM.68.1.53-58.2002 11772608 (Pubitemid 34031162)
17. The H2-sensing complex of Ralstonia eutropha: interaction between a regulatory [NiFe] hydrogenase and a histidine protein kinase. Buhrke T, Lenz O, Porthun A, Friedrich B, Mol Microbiol 2004 51 6 1677 1689 10.1111/j.1365-2958. 2003.03933.x 15009894 (Pubitemid 38406541)
18. The surprising diversity of clostridial hydrogenases: a comparative genomic perspective. Calusinska M, Happe T, Joris B, Wilmotte A, Microbiology 2010 156 Pt 6 1575 1588 20395274
19. A multisubunit membrane-bound [NiFe] hydrogenase and an NADH-dependent Fe-only hydrogenase in the fermenting bacterium Thermoanaerobacter tengcongensis. Soboh B, Linder D, Hedderich R, Microbiology 2004 150 Pt 7 2451 2463 15256587 (Pubitemid 39117657)
20. Clostridium thermocellum ATCC 27405 transcriptonomic, metabolomic, and proteomic profiles after ethanol stress. Yang S, Giannone RJ, Dice L, Yang ZK, Engle NL, Tschaplinski JT, Hettich RL, Brown SD, BMC Genomics 2012 13 335 n press
21. Lactate formation in Caldicellulosiruptor saccharolyticus is regulated by the energy carriers pyrophosphate and ATP. Willquist K, van Niel EW, Metab Eng 2010 12 3 282 290 10.1016/j.ymben.2010.01.001 20060925
22. Pyruvate catabolism and hydrogen synthesis pathway genes of Clostridium thermocellum ATCC 27405. Carere CR, Kalia V, Sparling R, Cicek N, Levin DB, Indian J Microbiol 2008 48 252 266 10.1007/s12088-008-0036-z
23. Purification and characterization of acetate kinase from Clostridium thermocellum. Lin WR, Peng Y, Lew S, Lee CC, Hsu JJ, Jean-Francois H, Demain AL, Tetrahedron 1988 54 15915 15925
24. Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization. Ozkan M, Yilmaz EI, Lynd LR, Ozcengiz G, Can J Microbiol 2004 50 10 845 851 10.1139/w04-071 15644899 (Pubitemid 40293247)
25. Regulation of the cellulosomal CelS (cel48A) gene of Clostridium thermocellum is growth rate dependent. Dror TW, Morag E, Rolider A, Bayer EA, Lamed R, Shoham Y, J Bacteriol 2003 185 10 3042 3048 10.1128/JB.185.10.3042- 3048.2003 12730163 (Pubitemid 36539100)
26. Regulation of expression of scaffoldin-related genes in Clostridium thermocellum. Dror TW, Rolider A, Bayer EA, Lamed R, Shoham Y, J Bacteriol 2003 185 17 5109 5116 10.1128/JB.185.17.5109-5116.2003 12923083 (Pubitemid 37021972)
27. Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs from that of a prominent cellulosomal xylanase. Dror TW, Rolider A, Bayer EA, Lamed R, Shoham Y, J Bacteriol 2005 187 7 2261 2266 10.1128/JB.187.7.2261-2266.2005 15774868 (Pubitemid 40389124)
28. Transcription of Clostridium thermocellum endoglucanase genes celF and celD. Mishra S, Beguin P, Aubert JP, J Bacteriol 1991 173 1 80 85 1987137
29. Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis. Gold ND, Martin VJ, J Bacteriol 2007 189 19 6787 6795 10.1128/JB.00882-07 17644599 (Pubitemid 47525422)
30. Impact of pretreated Switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a quantitative proteomic analysis. Raman B, Pan C, Hurst GB, Rodriguez M Jr, McKeown CK, Lankford PK, Samatova NF, Mielenz JR, PLoS One 2009 4 4 5271 10.1371/journal.pone.0005271 19384422
31. Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum. Zhang YH, Lynd LR, J Bacteriol 2005 187 1 99 106 10.1128/JB.187.1.99-106.2005 15601693 (Pubitemid 40024185)
32. Bacterial cellulose hydrolysis in anaerobic environmental subsystems-Clostridium thermocellum and Clostridium stercorarium, thermophilic plant-fiber degraders. Zverlov VV, Schwarz WH, Ann N Y Acad Sci 2008 1125 298 307 10.1196/annals.1419.008 18378600 (Pubitemid 351451154)
33. The unique set of putative membrane-associated anti-sigma factors in Clostridium thermocellum suggests a novel extracellular carbohydrate-sensing mechanism involved in gene regulation. Kahel-Raifer H, Jindou S, Bahari L, Nataf Y, Shoham Y, Bayer EA, Borovok I, Lamed R, FEMS Microbiol Lett 2010 308 1 84 93 10.1111/j.1574-6968.2010.01997.x 20487018
34. Clostridium thermocellum cellulosomal genes are regulated by extracytoplasmic polysaccharides via alternative sigma factors. Nataf Y, Bahari L, Kahel-Raifer H, Borovok I, Lamed R, Bayer EA, Sonenshein AL, Shoham Y, Proc Natl Acad Sci U S A 2009 107 43 18646 18651
35. Expression of 17 genes in Clostridium thermocellum ATCC 27405 during fermentation of cellulose or cellobiose in continuous culture. Stevenson DM, Weimer PJ, Appl Environ Microbiol 2005 71 8 4672 4678 10.1128/AEM.71.8.4672- 4678.2005 16085862 (Pubitemid 41129524)
36. Global gene expression patterns in Clostridium thermocellum as determined by microarray analysis of chemostat cultures on cellulose or cellobiose. Riederer A, Takasuka TE, Makino S, Stevenson DM, Bukhman YV, Elsen NL, Fox BG, Appl Environ Microbiol 2011 77 4 1243 1253 10.1128/AEM.02008-10 21169455
37. Transcriptomic analysis of Clostridium thermocellum ATCC 27405 cellulose fermentation. Raman B, McKeown CK, Rodriguez M Jr, Brown SD, Mielenz JR, BMC Microbiol 2011 11 134 10.1186/1471-2180-11-134 21672225
38. Carbon and electron flow in Clostridium cellulolyticum grown in chemostat culture on synthetic medium. Guedon E, Payot S, Desvaux M, Petitdemange H, J Bacteriol 1999 181 10 3262 3269 10322031 (Pubitemid 29236935)
39. Sander R, Compilation of Henry's law constants for inorganic and organic species of potential importance in environmental chemistry (Version 3) Air chemistry department, Max-Planck Institute of Chemistry, Mainz, Germany 1999 23035638
40. Darrett RH, Grisham CM, Biochemistry Saunders College Publishing, New York, NY 1995
41. Shotgun proteomics using the iTRAQ isobaric tags. Aggarwal K, Choe LH, Lee KH, Brief Funct Genomic Proteomic 2006 5 2 112 120 10.1093/bfgp/ell018 16772272 (Pubitemid 46403004)
42. A perspective on the use of iTRAQ reagent technology for protein complex and profiling studies. Zieske LR, J Exp Bot 2006 57 7 1501 1508 10.1093/jxb/erj168 16574745
43. Two-dimensional separation of peptides using RP-RP-HPLC system with different pH in first and second separation dimensions. Gilar M, Olivova P, Daly AE, Gebler JC, J Sep Sci 2005 28 14 1694 1703 10.1002/jssc.200500116 16224963 (Pubitemid 43055013)
44. Practical implementation of 2D HPLC scheme with accurate peptide retention prediction in both dimensions for high-throughput bottom-up proteomics. Dwivedi RC, Spicer V, Harder M, Antonovici M, Ens W, Standing KG, Wilkins JA, Krokhin OV, Anal Chem 2008 80 18 7036 7042 10.1021/ac800984n 18686972
45. Probability-based protein identification by searching sequence databases using mass spectrometry data. Perkins DN, Pappin DJ, Creasy DM, Cottrell JS, Electrophoresis 1999 20 18 3551 3567 10.1002/(SICI)1522-2683(19991201)20: 18<3551::AID-ELPS3551>3.0.CO;2-2 10612281 (Pubitemid 30007252)
46. ProteoWizard: open source software for rapid proteomics tools development. Kessner D, Chambers M, Burke R, Agus D, Mallick P, Bioinformatics 2008 24 21 2534 2536 10.1093/bioinformatics/btn323 18606607
47. Open source system for analyzing, validating, and storing protein identification data. Craig R, Cortens JP, Beavis RC, J Proteome Res 2004 3 6 1234 1242 10.1021/pr049882h 15595733 (Pubitemid 40040378)
48. Information-dependent LC-MS/MS acquisition with exclusion lists potentially generated on-the-fly: Case study using a whole cell digest of Clostridium thermocellum. McQueen P, Spicer V, Rydzak T, Sparling R, Levin D, Wilkins JA, Krokhin O, Proteomics 2012 12 1 10 22223622
49. The Paragon Algorithm, a next generation search engine that uses sequence temperature values and feature probabilities to identify peptides from tandem mass spectra. Shilov IV, Seymour SL, Patel AA, Loboda A, Tang WH, Keating SP, Hunter CL, Nuwaysir LM, Schaeffer DA, Mol Cell Proteomics 2007 6 9 1638 1655 10.1074/mcp.T600050-MCP200 17533153 (Pubitemid 47506173)
50. Ethanol production by thermophilic bacteria: relationship between fermentation product yields of and catabolic enzyme activities in Clostridium thermocellum and Thermoanaerobium brockii. Lamed R, Zeikus JG, J Bacteriol 1980 144 2 569 578 7430065 (Pubitemid 11164174)
51. Growth of the thermophilic bacterium Clostridium thermocellum on continuous culture. Strobel HJ, Curr Microbiol 1995 31 210 214 10.1007/BF00298375
52. Ethanol production by thermophilic bacteria: metabolic control of end product formation in Thermoanaerobium brockii. Ben-Bassat A, Lamed R, Zeikus JG, J Bacteriol 1981 146 1 192 199 7217000 (Pubitemid 11116472)
53. Effects of Stirring and Hydrogen on Fermentation Products of Clostridium thermocellum. Lamed RJ, Lobos JH, Su TM, Appl Environ Microbiol 1988 54 5 1216 1221 16347632
54. Metabolic selectivity and growth of Clostridium thermocellum in continuous culture under elevated hydrostatic pressure. Bothun GD, Knutson BL, Berberich JA, Strobel HJ, Nokes SE, Appl Microbiol Biotechnol 2004 65 149 157 14752579 (Pubitemid 39062476)
55. End-product induced metabolic shifts in Clostridium thermocellum ATCC 27405. Rydzak T, Levin DB, Cicek N, Sparling R, Appl Microbiol Biotechnol 2011 92 1 199 209 10.1007/s00253-011-3511-0 21837436
56. Continuous hydrogen production during fermentation of alpha-cellulose by the thermophillic bacterium Clostridium thermocellum. Magnusson L, Cicek N, Sparling R, Levin D, Biotechnol Bioeng 2009 102 3 759 766 10.1002/bit.22092 18828175
57. A model for random sampling and estimation of relative protein abundance in shotgun proteomics. Liu H, Sadygov RG, Yates JR 3rd, Anal Chem 2004 76 14 4193 4201 10.1021/ac0498563 15253663 (Pubitemid 38943698)
58. Comparison of label-free methods for quantifying human proteins by shotgun proteomics. Old WM, Meyer-Arendt K, Aveline-Wolf L, Pierce KG, Mendoza A, Sevinsky JR, Resing KA, Ahn NG, Mol Cell Proteomics 2005 4 10 1487 1502 10.1074/mcp.M500084-MCP200 15979981 (Pubitemid 41555475)
59. Mass spectrometry-based label-free quantitative proteomics. Zhu W, Smith JW, Huang CM, J Biomed Biotechnol 2010 2010 840518 19911078
60. Correlation of relative abundance ratios derived from peptide ion chromatograms and spectrum counting for quantitative proteomic analysis using stable isotope labeling. Zybailov B, Coleman MK, Florens L, Washburn MP, Anal Chem 2005 77 19 6218 6224 10.1021/ac050846r 16194081 (Pubitemid 41436955)
61. Large-scale proteomic analysis of the human spliceosome. Rappsilber J, Ryder U, Lamond AI, Mann M, Genome Res 2002 12 8 1231 1245 10.1101/gr.473902 12176931 (Pubitemid 41264425)
62. Proteomic analysis by multidimensional protein identification technology. Florens L, Washburn MP, Methods Mol Biol 2006 328 159 175 16785648
63. Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae. Zybailov B, Mosley AL, Sardiu ME, Coleman MK, Florens L, Washburn MP, J Proteome Res 2006 5 9 2339 2347 10.1021/pr060161n 16944946 (Pubitemid 44330827)
64. Cellulase, clostridia, and ethanol. Demain AL, Newcomb M, Wu JH, Microbiol Mol Biol Rev 2005 69 1 124 154 10.1128/MMBR.69.1.124-154.2005 15755956 (Pubitemid 40360711)
65. Pectinolytic activity of Clostridium thermocellum: Its use for anaerobic fermentation of sugar beet pulp. Spinnler HE, Lavigne B, Blanchere H, Appl Microbiol Biotechnol 1986 23 434 437 10.1007/BF02346055 (Pubitemid 16145717)
66. Co-transcription of the celC gene cluster in Clostridium thermocellum. Newcomb M, Millen J, Chen CY, Wu JH, Appl Microbiol Biotechnol 2011 90 2 625 634 10.1007/s00253-011-3121-x 21318364
67. Induction of the celC operon of Clostridium thermocellum by laminaribiose. Newcomb M, Chen CY, Wu JH, Proc Natl Acad Sci U S A 2007 104 10 3747 3752 10.1073/pnas.0700087104 17360424 (Pubitemid 47181526)
68. Carbohydrate transport by the anaerobic thermophile Clostridium thermocellum LQRI. Strobel HJ, Caldwell FC, Dawson KA, Appl Environ Microbiol 1995 61 11 4012 4015 16535164
69. Cellodextrin efflux by the cellulolytic ruminal bacterium Fibrobacter succinogenes and its potential role in the growth of nonadherent bacteria. Wells JE, Russell JB, Shi Y, Weimer PJ, Appl Environ Microbiol 1995 61 5 1757 1762 7646013
70. Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation. Zhang YH, Lynd LR, Proc Natl Acad Sci U S A 2005 102 20 7321 7325 10.1073/pnas.0408734102 15883376 (Pubitemid 40696377)
71. Bacterial glycogen synthesis and its regulation. Preiss J, Annu Rev Microbiol 1984 38 419 458 10.1146/annurev.mi.38.100184.002223 6093684
72. Physiology, biochemistry and genetics of bacterial glycogen synthesis. Preiss J, Romeo T, Adv Microb Physiol 1989 30 183 238 2700539
73. Kinetic analysis of Clostridium cellulolyticum carbohydrate metabolism: importance of glucose 1-phosphate and glucose 6-phosphate branch points for distribution of carbon fluxes inside and outside cells as revealed by steady-state continuous culture. Guedon E, Desvaux M, Petitdemange H, J Bacteriol 2000 182 7 2010 2017 10.1128/JB.182.7.2010-2017.2000 10715010 (Pubitemid 30165392)
74. Cell population heterogeneity during growth of Bacillus subtilis. Kearns DB, Losick R, Genes Dev 2005 19 24 3083 3094 10.1101/gad.1373905 16357223 (Pubitemid 41798122)
75. ATP versus pyrophosphate: glycolysis revisited in parasitic protists. Mertens E, Parasitol Today 1993 9 4 122 126 10.1016/0169-4758(93)90169-G 15463728 (Pubitemid 23080753)
76. Pyrophosphate-dependent phosphofructokinase from the amoeba Naegleria fowleri, an AMP-sensitive enzyme. Mertens E, De Jonckheere J, Van Schaftingen E, Biochem J 1993 292 Pt 3 797 803 8391256 (Pubitemid 23210212)
77. A pathway for the interconversion of hexose and pentose in the parasitic amoeba Entamoeba histolytica. Susskind BM, Warren LG, Reeves RE, Biochem J 1982 204 1 191 196 6180735
78. Comparative Genomics and Bioenergetics of Dark Fermentation (Chapter 10). Sparling R, Carere C, Rydzak T, Schellenberg J, Levin D, State of the Art and Progress in Production of Biohydrogen Bentham eBooks, Sharjah, UAE, Azbar N, Levin DB, 2012 160 188
79. Thermostable, ammonium-activated malic enzyme of Clostridium thermocellum. Lamed R, Zeikus JG, Biochim Biophys Acta 1981 660 2 251 255 10.1016/0005-2744(81)90167-4 7284402
80. Genome-scale metabolic model integrated with RNAseq data to identify metabolic states of Clostridium thermocellum. Gowen CM, Fong SS, Biotechnol J 2010 5 7 759 767 10.1002/biot.201000084 20665646
81. Purification and characterization of the pyruvate-ferredoxin oxidoreductase from Clostridium acetobutylicum. Meinecke B, Bertram J, Gottschalk G, Arch Microbiol 1989 152 3 244 250 10.1007/BF00409658 2774799
82. Influence of process conditions on end product formation from Clostridium thermocellum 27405 in solid substrate cultivation on paper pulp sludge. Chinn MS, Nokes SE, Strobel HJ, Bioresour Technol 2007 98 11 2184 2193 10.1016/j.biortech.2006.08.033 17107786 (Pubitemid 46402971)
83. Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12. Sawers G, Bock A, J Bacteriol 1988 170 11 5330 5336 3053657
84. Structural basis for glycyl radical formation by pyruvate formate-lyase activating enzyme. Vey JL, Yang J, Li M, Broderick WE, Broderick JB, Drennan CL, Proc Natl Acad Sci U S A 2008 105 42 16137 16141 10.1073/pnas.0806640105 18852451
85. Molecular characteristics and transcription of the gene encoding a multifunctional alcohol dehydrogenase in relation to the deactivation of pyruvate formate-lyase in the ruminal bacterium Streptococcus bovis. Asanuma N, Yoshii T, Hino T, Arch Microbiol 2004 181 2 122 128 10.1007/s00203-003-0638-0 14676990 (Pubitemid 38221648)
86. Properties of acetate kinase activity in Clostridium thermocellum cell extracts. Lin WR, Lee CC, Hsu JJ, Hamel JF, Demain AL, Appl Biochem Biotechnol 1998 69 2 137 145 10.1007/BF02919395 18574732 (Pubitemid 28371379)
87. The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production. Schut GJ, Adams MW, J Bacteriol 2009 191 13 4451 4457 10.1128/JB.01582-08 19411328
88. Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout. Shaw AJ, Hogsett DA, Lynd LR, J Bacteriol 2009 191 20 6457 6464 10.1128/JB.00497-09 19648238
89. Induction of lactate production associated with a decrease in NADH cell content enables growth resumption of Clostridium cellulolyticum in batch cultures on cellobiose. Payot S, Guedon E, Gelhaye E, Petitdemange H, Res Microbiol 1999 150 7 465 473 10.1016/S0923-2508(99)00110-2 10540910
90. Metabolic flux in cellulose batch and cellulose-fed continuous cultures of Clostridium cellulolyticum in response to acidic environment. Desvaux M, Guedon E, Petitdemange H, Microbiology 2001 147 Pt 6 1461 1471 11390677 (Pubitemid 32547220)
91. A hydrogen-sensing multiprotein complex controls aerobic hydrogen metabolism in Ralstonia eutropha. Friedrich B, Buhrke T, Burgdorf T, Lenz O, Biochem Soc Trans 2005 33 Pt 1 97 101 15667276 (Pubitemid 40313771)
92. The H(2) sensor of Ralstonia eutropha is a member of the subclass of regulatory [NiFe] hydrogenases. Kleihues L, Lenz O, Bernhard M, Buhrke T, Friedrich B, J Bacteriol 2000 182 10 2716 2724 10.1128/JB.182.10.2716-2724.2000 10781538 (Pubitemid 30246900)
93. Thermoanaerobacter spp. control ethanol pathway via transcriptional regulation and versatility of key enzymes. Pei J, Zhou Q, Jiang Y, Le Y, Li H, Shao W, Wiegel J, Metab Eng 2010 12 5 420 428 10.1016/j.ymben.2010.06.001 20547239
94. Distribution of heat labile and heat stable inorganic pyrophosphatase. Blumenthal M, Johnson MK, Johnson EJ, Can J Microbiol 1967 13 12 1695 1699 10.1139/m67-219 4868878
95. Thermotoga maritima phosphofructokinases: expression and characterization of two unique enzymes. Ding YR, Ronimus RS, Morgan HW, J Bacteriol 2001 183 2 791 794 10.1128/JB.183.2.791-794.2001 11133978 (Pubitemid 32048142)
96. Phosphotransacetylase from Clostridium acidiurici. Robinson JR, Sagers RD, J Bacteriol 1972 112 1 465 473 16559158
97. Physiological characteristics of the extreme thermophile Caldicellulosiruptor saccharolyticus: an efficient hydrogen cell factory. Willquist K, Zeidan AA, van Niel EW, Microb Cell Fact 2010 9 89 10.1186/1475-2859-9-89 21092203
98. Heinonen J.K. Drake H.L. Comparative assessment of inorganic pyrophosphate and pyrophosphatase levels of Escherichia coli, Clostridium pasteurianum, and Clostridium thermoaceticum FEMS Microbiol Lett 1988 52 205-208 10.1111/j.1574-6968.1988.tb02596.x