Carbon and electron flow in Clostridium cellulolyticum grown in chemostat culture on synthetic medium

Journal of Bacteriology

Volumn 181, Issue 10, 1999, Pages 3262-3269

  Guedon, E ^a   Payot, S ^a   Desvaux, M ^a   Petitdemange, H ^a  

^a UNIVERSITÉ DE LORRAINE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; ALCOHOL DEHYDROGENASE; CARBON; CELLOBIOSE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; LACTATE DEHYDROGENASE; LACTIC ACID; PYRUVIC ACID;

ARTICLE; BACTERIAL METABOLISM; BIOMASS; CARBON DIOXIDE FIXATION; CATABOLISM; CELL LEVEL; CELL TRANSPORT; CHEMOSTAT; CLOSTRIDIUM CELLULOLYTICUM; CULTURE MEDIUM; ELECTRON TRANSPORT; ENZYME ACTIVITY; FERMENTATION; GLYCOLYSIS; METABOLIC RATE; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM CELLULOLYTICUM; NEGIBACTERIA; TRIXIS;

EID: 0032912348     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.10.3262-3269.1999     Document Type: Article

References (45)

1. Bayer, E. A., Y. Shoham, J. Tormo, and R. Lamed. 1996. The cellulosome: a cell surface organelle for the adhesion to and degradation of cellulose, p. 155-182. In M. Fletcher (ed.), Bacterial adhesion molecular and ecological diversity. Wiley-Liss, New York, N.Y.
2. Bayer, E. A., E. Morag, and R. Lamed. 1994. The cellulosome - a treasuretrove for biotechnology. Trends Biotechnol. 12:379-386.
3. Béguin, P., and J. P. Aubert. 1994. The biological degradation of cellulose. FEMS Microbiol. Rev. 13:25-28.
4. Benoit, L., C. Cailliez, E. Petitdemange, and J. Gitton. 1992. Isolation of cellulolytic mesophilic clostridia from a municipal solid waste digestor. Microb. Ecol. 23:117-125.
5. Bradford, M. M. 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
6. Bryant, M. P. 1972. Commentary on the Hungate technique for culture of anaerobic bacteria. Am. J. Clin. Nutr. 25:1324-1328.
7. Cailliez, C., L. Benoit, J. P. Thirion, and H. Petitdemange. 1992. Characterization of 10 mesophilic cellulolytic clostridia isolated from a municipal solid waste digestor. Curr. Microbiol. 25:105-112.
8. Chaney, A. L., and E. P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8:130-132.
9. Chen, J. S., and L. E. Mortenson. 1974. Purification and properties of hydrogenase from Clostridium pasteurianum W5. Biochim. Biophys. Acta 371:283-298.
10. 2 on growth of Clostridium cellobioparum. Appl. Environ. Microbiol. 31:342-348.
11. Church, F. C., H. P. David, G. L. Catagniani, and H. G. Swaigsgood. 1985. An O-phthalaldehyde spectrometric assay for proteinase. Anal. Biochem. 146:343-348.
12. Felix, C. R., and L. G. Ljungdahl. 1993. The cellulosome: the exocellular organelle of Clostridium. Annu. Rev. Microbiol. 47:791-819.
13. Gelhaye, E., A. Gehin, and H. Petitdemange. 1993. Colonization of crystalline cellulose by Clostridium cellulolyticum ATCC 35319. Appl. Environ. Microbiol. 59:3154-3156.
14. Gelhaye, E., H. Petitdemange, and R. Gay. 1993. Adhesion and growth rate of Clostridium cellulolyticum ATCC 35319 on crystalline cellulose. J. Bacteriol. 175:3452-3458.
15. Giallo, J., C. Gaudin, J. P. Belaich, E. Petitdemange, and F. Caillet-Mangin. 1983. Metabolism of glucose and cellobiose by cellulolytic mesophilic Clostridium sp. strain H10. Appl. Environ. Microbiol. 45:843-849.
16. Giallo, J., C. Gaudin, and J. P. Belaich. 1985. Metabolism and solubilization of cellulose by Clostridium cellulolyticum H10. Appl. Environ. Microbiol. 45:1215-1221.
17. Giles, K. W., and A. Myers. 1965. An improved diphenylamine method for the estimation of deoxyribonucleic acid. Nature 206:93.
18. Huang, L., and C. W. Forsberg. 1990. Cellulose digestion and cellulase regulation and distribution in Fibrobacler succinogenes subsp. succinogenes S85. Appl. Environ. Microbiol. 56:1221-1228.
19. Hungate, R. E. 1950. The anaerobic mesophilic cellulolytic bacteria. Bacteriol. Rev. 14:1-49.
20. Hungate, R. E. 1969. A roll tube method for cultivation of strict anaerobes. Methods Microbiol. 33:117-132.
21. +, NADH, NADPH). Spectrophotometric and fluorimetric methods, p. 2045-2059. In H. Y. Bergmeyer (ed.), Methods in enzymatic analysis. Academic Press, Inc., New York, N.Y.
22. Koch, A. L. 1997. Microbial physiology and ecology of slow growth. Microbiol. Mol. Biol. Rev. 61:305-318.
23. Leschine, S. B. 1995. Cellulose degradation in anaerobic environments. Annu. Rev. Microbiol. 49:399-426.
24. London, J., and M. Knight. 1966. Concentrations of nicotinamide nucleotide coenzymes in micro-organisms. J. Gen. Microbiol. 44:241-254.
25. Lovenberg, W., B. B. Buchanan, and J. C. Rabinowitz. 1963. Studies on the chemical nature of clostridial ferredoxin. J. Biol. Chem. 238:3899-3913.
26. Lowry, O. H., N. J. Rosebrough, A. L. Fair, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
27. Maglione, G., and J. B. Russel. 1997. The adverse effect of nitrogen limitation and excess cellobiose on Fibrobacler succinogenes S85. Appl. Microbiol. Biotechnol. 48:720-725.
28. Meinecke, B., J. Bertram, and G. Gottschalk. 1989. Purification and characterization of the pyruvate-ferredoxin oxidoreductase from Clostridium acetobutylicum. Arch. Microbiol. 152:244-250.
29. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31:426-428.
30. Miller, G. L., R. Blum, W. Glennon, and A. L. Burton. 1960. Measurement of carboxymethylcellulase activity. Anal. Chem. 138:481-487.
31. Mortenson, L. E., R. C. Valentine, and J. E. Carnahan. 1963. Ferredoxin in the phosphoroclastic reaction of pyruvic acid and its relation to nitrogen fixation in Clostridium pasteurianum. J. Biol. Chem. 238:794-800.
32. Ng, T., and J. G. Zeikus. 1982. Differential metabolism of cellobiose and glucose by Clostridium thermocellum and Clostridium thermohydrosulfuricum. J. Bacteriol. 150:1391-1399.
33. Papoutsakis, E. T., and C. L. Meyer. 1985. Equations and calculations of product yields and preferred pathways for butanediol and mixed-acid fermentations. Biotechnol. Bioeng. 27:50-66.
34. Payot, S., E. Guedon, C. Cailliez, E. Gelhaye, and H. Petitdemange. 1998. Metabolism of cellobiose by Clostridium cellulolyticum growing in continuous culture: evidence for decreased NADH reoxidation as a factor limiting growth. Microbiology 144:375-384.
35. Petitdemange, E., F. Caillet, J. Giallo, and C. Gaudin. 1984. Clostridium cellulolyticum sp. nov., a cellulolytic mesophilic species from decayed grass. Int. J. Syst. Bacteriol. 34:155-159.
36. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
37. Shi, Y., and P. J. Weimer. 1996. Utilization of individual cellodextrins by three predominant ruminal cellulolytic bacteria. Appl. Environ. Microbiol. 62:1084-1088.
38. Sleat, R., R. Mah, and R. Robinson. 1984. Isolation and characterization of an anaerobic, cellulolytic bacterium, Clostridium cellulovorans sp. nov. Appl. Environ. Microbiol. 48:88-93.
39. Strobel, H. J., F. C. Caldwell, and K. A. Dawson. 1995. Carbohydrate transport by the anaerobic thermophile Clostridium thermocellum LQRI. Appl. Environ. Microbiol. 61:4012-4015.
40. Thomas, T. D., D. C. Elwood, and M. C. Longyear. 1979. Change from homoto heterolactic fermentation by Streptococcus lactis resulting from glucose limitation in anaerobic chemostat cultures. J. Bacteriol. 138:109-117.
41. Thompson, J., and T. D. Thomas. 1977. Phosphoenolpyruvate and 2-phosphoglycerate: endogenous energy source(s) for sugar accumulation by starved cells of Streptococcus lactis. J. Bacteriol. 130:583-595.
42. Tomme, P., R. A. J. Warren, and N. R. Gilkes. 1995. Cellulose hydrolysis by bacteria and fungi. Adv. Microb. Physiol. 37:1-81.
43. Uyeda, K., and J. C. Rabinowitz. 1971. Pyruvate-ferredoxin oxidoreductase. Purification and properties of the enzyme. J. Biol. Chem. 246:3111-3119.
44. Weimer, P. J., and J. G. Zeikus. 1977. Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence and presence of Methanobacterium thermoautotrophicum. Appl. Environ. Microbiol. 33:289-297.
45. Wimpenny, J. W. T., and A. Firth. 1972 Levels of nicotinamide adenine dinucleotide and reduced nicotinamide adenine dinucleotide in facultative bacteria and the effect of oxygen. J. Bacteriol. 111:24-32.