Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization

Canadian Journal of Microbiology

Volumn 50, Issue 10, 2004, Pages 845-851

  Özkan, Melek ^a   Yilmaz, Ebru I ^a,b   Lynd, Lee R ^c   Özcengiz, Gülay ^a  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

^b DICLE UNIVERSITY   (Turkey)

^c DARTMOUTH COLLEGE   (United States)

Author keywords

L lactate dehydrogenase purification; Thermophilic bacteria

Indexed keywords

CATALYSIS; DNA; ENZYME KINETICS; ESCHERICHIA COLI; GENES; PROTEINS;

ALLOSTERIC ENZYMES; CATALYTIC ACTIVITY; GENE ENCODING; GENE EXPRESSIONS;

CLONING;

FRUCTOSE 1,6 BISPHOSPHATE; GENOMIC DNA; LACTATE DEHYDROGENASE; LACTIC ACID; PYRUVIC ACID;

ENZYME; GENE; MICROBIOLOGY;

ALLOSTERISM; ARTICLE; BACTERIOPHAGE LAMBDA; BASE PAIRING; CATALYSIS; CLOSTRIDIUM THERMOCELLUM; ENZYME ACTIVATION; ENZYME ANALYSIS; ENZYME KINETICS; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; STRUCTURAL GENE;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; CLOSTRIDIUM THERMOCELLUM; ENZYME ACTIVATION; ENZYME ACTIVATORS; ENZYME STABILITY; ESCHERICHIA COLI; FRUCTOSEDIPHOSPHATES; GENE EXPRESSION REGULATION, BACTERIAL; L-LACTATE DEHYDROGENASE; LACTIC ACID; MOLECULAR SEQUENCE DATA; MUTATION; OPEN READING FRAMES; PROMOTER REGIONS (GENETICS); PYRUVIC ACID; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 14344260653     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w04-071     Document Type: Article

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