Proteomic and Systems Biology Analysis of Monocytes Exposed to Securinine, a GABAA Receptor Antagonist and Immune Adjuvant

PLoS ONE

Volumn 7, Issue 9, 2012, Pages

  Shipman, Matt ^a   Lubick, Kirk ^a   Fouchard, David ^a   Guram, Rajani ^a   Grieco, Paul ^a   Jutila, Mark ^a   Dratz, Edward A ^a  

^a MONTANA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; ACTIN; ACTIN BINDING PROTEIN; CALVASCULIN; FATTY ACID BINDING PROTEIN; FATTY ACID BINDING PROTEIN 5; HEAT SHOCK PROTEIN 60; HEAT SHOCK PROTEIN 70; HEAT SHOCK PROTEIN 90; IMMUNOLOGICAL ADJUVANT; INOSINATE DEHYDROGENASE; L PLASTIN; MITOGEN ACTIVATED PROTEIN KINASE P38; PROTEOME; SECURININE; UNCLASSIFIED DRUG;

ARTICLE; BIOINFORMATICS; CELL METABOLISM; CONTROLLED STUDY; COXIELLA BURNETII; ENZYME ACTIVATION; FUNCTIONAL PROTEOMICS; HORMONAL REGULATION; IMMUNOSTIMULATION; INFLAMMATION; LIPID COMPOSITION; LIPID RAFT; METABOLIC REGULATION; MONOCYTE; PROTEIN EXPRESSION; PROTEIN FUNCTION; Q FEVER; RECEPTOR BLOCKING; SYSTEMS BIOLOGY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ADJUVANTS, IMMUNOLOGIC; ANTIGEN PRESENTATION; AZEPINES; COMPUTATIONAL BIOLOGY; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GABA-A RECEPTOR ANTAGONISTS; HETEROCYCLIC COMPOUNDS WITH 4 OR MORE RINGS; HSP70 HEAT-SHOCK PROTEINS; HSP90 HEAT-SHOCK PROTEINS; LACTONES; METABOLIC NETWORKS AND PATHWAYS; MODELS, BIOLOGICAL; MONOCYTES; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PIPERIDINES; PROTEOME; PROTEOMICS; RECEPTORS, GABA-A; SIGNAL TRANSDUCTION;

COXIELLA BURNETII;

EID: 84866412649     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0041278     Document Type: Article

References (106)

1. Lubick K, Radke M, Jutila M, (2007) Securinine, a GABAA receptor antagonist, enhances macrophage clearance of phase II C. burnetii: comparison with TLR agonists. The Journal of Leukocyte Biology 82: 1062-1069.
2. Beutler JA, Karbon EW, Brubaker AN, Malik R, Curtis DR, et al. (1985) Securinine alkaloids: a new class of GABA receptor antagonist. Brain Research 330: 135-140.
3. Alam S, Laughton DL, Walding A, Wolstenholme AJ, (2006) Human peripheral blood mononuclear cells express GABAA receptor subunits. Molecular Immunology 43: 1432-1442.
4. Reyes-García MG, Hernández-Hernández Fl, Hernádex-Téllez B, García-Tamayo F, (2007) GABAA receptor subunits RNA expression in mice peritoneal macrophages modulate their IL-6/IL-12 production. Journal of Neuroimmunology 188: 64-68.
5. Bjurstöm H, Wang J-Y, Ericsson I, Bengtsson M, Liu Y, et al. (2008) GABA, a natural immunomodulator of T lymphocytes. Journal of Neuroimmunology 205: 44-50.
6. Gadgil HS, Pabst KM, Giorgianni F, Unstot ES, Desiderio DM, et al. (2003) Proteome of monocytes primed with lipopolysaccharide: analysis of the abundant proteins. Proteomics 3: 1767-1780.
7. Pabst MJ, Pabst KM, Handsman DB, Beranova-Giorgianni S, Giorgianni F, (2008) Proteome of monocyte priming by lipopolysaccharide, including changes in interleukin-1 beta and leukocyte elastase inhibitor. Proteome Science 6: 13.
8. Baca OG, Roman MJ, Glew RH, Christner RF, Buhler JE, et al. (1993) Acid phosphatase activity in Coxiella burnetii. Infection and Immunity 61: 4232-4239.
9. Samoilis G Psaroulake A, Vougas K, Tselentis Y, Tsiotis G, (2007) Analysis of whole cell lysate from the intercellular bacterium Coxiella burnetii using two gel-based protein separation techniques. Journal of Proteome Research 6: 3032-3041.
10. Dratz EA, Grieco PA. Novel Zwitterionic Fluorescent Dyes for Labeling in Proteomic and Other Biological Analyses. USA patent issued: Number 7,582,260, 9/1/2009, Montana State University.
11. Dratz EA, Grieco PA. Novel Zwitterionic Fluorescent Dyes for Labeling in Proteomic and Other Biological Analyses. Methods Claims. USA patent issued, Number 7,833,799, Montana State University, 11/16/2010.
12. Dratz EA, Grieco PA. Novel Optical Labeling Molecules for Proteomics and Other Biological Analyses, Montana State University, PCT application US2008/059963.
13. Barry R (2003) Proteomic Analysis of Sulfolobus solfataricus, an Extremophilic Archaeon. Bozeman: Montana State University.
14. Barry RC, Young MJ, Stedman KM, Dratz EA, (2006) Proteomic mapping of the hyperthermophilic and acidophilic archaeon Sulfolobus solfatarticus P2. Electrophoresis 27: 2970-2983.
15. Han J, Schey KL, (2004) Proteolysis and mass spectrometric analysis of an integral membrane: Aquaporin 0. Journal of Proteome Research 3: 807-812.
16. Perkins DN, Pappin DJC, Creasy DM, Cottrell JS, (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20: 3551-3567.
17. Craig R, Cortens JP, Beavis RC, (2004) Open source system for analyzing, validating, and storing protein identification data. Journal of Proteome Research 3: 1234-1242.
18. Craig R, Cortens JP, Beavis RC, (2005) The use of proteotypic peptide libraries for protein identification. Rapid Communications in Mass Specrometry 19: 1844-1850.
19. Craig R, Cortens JP, Fenyo D, Beavis RC, (2006) Using annotated peptide mass spectrum libraries for protein identification. Journal of Proteome Research 5: 1843-1849.
20. Fenyo D, Phinney BS, Beavis RC, (2007) Determining the overall merit of protein identification data sets: rho-diagrams and rho-scores. Journal of Proteome Research 6: 1997-2004.
21. Karp NA, Spencer M, Lindsay H, O'Dell K, Lilley KS, (2005) Impact of replicate types on proteomic expression analysis. Journal of Proteome Research 4: 1867-1871.
22. Markiel SP, Ozier O, Baliga NS, Wang JT, Ramage D, et al. (2003) Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Research 13: 2498-2504.
23. Huang DW, Sherman BT, Lempicki RA, (2009) Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nature Protocols 4: 44-57.
24. Dennis GJ, Sherman BT, Hosack DA, Yang J, Gao W, et al. (2003) DAVID: Database for annotation, visualization, and integrated discovery. Genome Biology 4: P3.
25. Zheng Q, Wang XJ, (2008) GOEAST: a web-based software toolkit for Gene Ontology enrichment analysis. Nucleic Acids Research 36: W358-63.
26. Chen H, Mocsai A, Zhang H, Ding R-X, Morisaki JH, et al. (2003) Role for plastin in host defense distinguishes integrin signaling from cell adhesion spreading. Immunity 19: 95-104.
27. Jones SL, Wang J, Turck CW, Brown EJ, (1998) A role for the actin-bundling protein L-plastin in the regulation of leukocyte integrin function. Proceedings of the National Academy of Sciences USA 95: 9331-9336.
28. Wabnitz GH, Köcher Tl, Lohneis P, Stober C, Konstandin MH, et al. (2007) Costimulation induced phosphorylation of L-plastin facilitates surface transport of the T cell activation molecules CD69 and CD25. European Journal of Immunology 37: 649-662.
29. Wang J, Brown EJ, (1999) Immune complex-induced integrin activation and L-plastin phosphorylation require protein kinase A. The Journal of Biological Chemistry 274: 24349-24356.
30. Managan MSJ, Kaiserman D, Bird PI, (2008) The role of serpins in vertebrate immunity. Tissue Antigens 72: 1-10.
31. Arnér ESJ, Holmgren A, (2000) Physiological functions of thioredoxin and thioredoxin reductase. European Journal of Biochemistry 267: 6102-6109.
32. Billiet L, Furman C, Larigauderie G, Copin C, Brand K, et al. (2005) Extracellular human thioredoxin-1 inhibits lipopolysaccharide-induced interleukin-1β expression in human monocyte-derived macrophages. The Journal of Biological Chemistry 280: 40310-40318.
33. Boye K, Grotterød I, Aasheim H-C, Hovig E, Mælandsmo GM, (2008) Activation of NF-kB by extracellular S100A4: analysis of signal transduction mechanisms and identification of target genes. International Journal of Cancer 123: 1301-1310.
34. Rajagopal D, Bal V, Mayor S, George A, Rath S, (2006) A role for Hsp90 molecular chaperone family in antigen presentation to T lymphocytes via Major Histocompatibility Complex Class II molecules. European Journal of Immunology 36: 828-841.
35. Hunter-Lavin C, Davies EL, Bacelar MMFVG, Marshall MJ, Andrew SM, et al. (2004) Hsp70 release from peripheral blood mononuclear cells. Biochemical and Biophysical Research Communications 324: 511-517.
36. Kol A, Lichtman AH, Finberg RW, Libby P, Kurt-Jones EA, (2000) Cutting edge: heat shock protein (HSP) 60 activates the innate immune response: CD14 is an essential receptor for HSP60 activation of mononuclear cells. Journal of Immunology 164: 13-17.
37. Maeda K, Cao H, Kono K, Gorgun CZ, Furuhashi M, et al. (2005) Adipocyte/macrophage fatty acid binding proteins control integrated metabolic responses in obesity and diabetes. Cell Metabolism 1: 107-119.
38. Maeda K, Uysal KT, Makowski L, Görgün CZ, Atsumi G, et al. (2003) Role of fatty acid binding protein mal1 in obesity and insulin resistance. Diabetes 52: 300-307.
39. Tan N-S, Shaw NS, Vinckenbosch N, Liu P, Yasmin R, et al. (2002) Selective cooperation between fatty acid binding proteins and peroxisome proliferator-activated receptors in regulating transcription. Molecular and Cellular Biology 22: 5114-5127.
40. Jain J, Almquist SJ, Ford PJ, Shlyakhter D, Wang Y, et al. (2004) Regulation of inosine monophosphate dehydrogenase type I and type II isoforms in human lymphocytes. Biochemical Pharmacology 67: 767-776.
41. Moosavi MA, Yazdanparast R, Sanati MH, Nejad AS, (2005) 3-hydrogenkwadaphnin targets inosine 5′-monophasphate dehydrogenase and triggers post-G1 arrest apoptosis in human leukemia cell lines. The International Journal of Biochemistry and Cell Biology 37: 2366-2379.
42. Pereira SA, Faca VM, Gomes GG, Chammas R, Fontes AM, et al. (2005) Changes in the proteomic profile during differentiation and maturation of human moncyte-derived dendritic cells stimulated with granulocyte macrophage colony stimulating factor/interleukin-4 and lipopolysaccharide. Proteomics 5: 1186-1198.
43. Furuhashi M, Hotamisligil GS, (2008) Fatty-acid binding proteins: role in metabolic diseases and potential as drug targets. Nature Reviews: Drug Discovery 7: 489-503.
44. Cao H, Maeda K, Gorgun CZ, Kim H-J, Park S-Y, et al. (2006) Regulation of metabolic responses by adipocyte/macrophage fatty acid-binding protens in leptin-deficient mice. Diabetes 55: 1915-1922.
45. Li N, Mak A, Richards DP, Naber C, Keller BO, et al. (2003) Monocyte lipid rafts contain proteins implicated in vesicular trafficking and phagosome formation. Proteomics 3: 536-548.
46. Bae T-J, Kim M-S, Kim J-W, Kim B-W, Choo H-J, et al. (2004) Lipid raft proteome reveals ATP synthase complex in the cell surface. Proteomics 4: 3536-3548.
47. Browman DT, Hoegg MB, Robbins SM, (2007) The SPFH domain-containing proteins: more than lipid raft markers. Trends in Cell Biology 17: 394-402.
48. Lebrand C, Corti M, Goodson H, Cosson P, Cavalli V, et al. (2002) Later endosome motility depends on lipids via the small GTPase Rab7. The EMBO Journal 21: 1289-1300.
49. Cao H, Gerhold K, Mayers JR, Wiest MM, Watkins SM, et al. (2008) Identification of a lipokine, a lipid hormone linking adipose tissue to systemic metabolism. Cell 134: 933-944.
50. Chavez-Salinas S, Ceballos-Olvera I, Reyes-del Valle J, Medina F, del Angel RM, (2008) Heat shock effect upon Dengue virus replication into U937 cells. Virus Research 138: 111-118.
51. Wang R, Kovalchin JT, Muhlenkamp P, Chandawarkar RY, (2006) Exogenous heat shock protein 70 binds macrophage lipid raft microdomain and stimulates phagocytosis, processing, and MHC-II presentation of antigens. Blood 107: 1636-1642.
52. Broquet AH, Thomas G, Masliah J, Trugnan G, Bachlet M, (2003) Expression of the molecular chaperone Hsp70 in detergent-resistant microdomains correlates with its membrane delivery and release. The Journal of Biological Chemistry 278: 21601-21606.
53. Ausiello CM, Fedele G, Palazzo R, Spensieri F, Ciervo A, et al. (2006) 60-kDa heat shock protein of Chlamydia pneumoniae promotes a T helper type 1 immune response through IL-12/IL-23 production in monocyte derived dendritic cells. Microbes and Infection 8: 714-720.
54. Osterloh A, Kalinke U, Weiss S, Fleischer B, Breloer M, (2007) Synergistic and differential modulation of immune responses by Hsp60 and lipopolysaccharide. The Journal of Biological Chemistry 282: 4669-4680.
55. Wang Y, Whittall T, McGowan E, Younson J, Kelly C, et al. (2005) Identification of stimulating and inhibitory epitopes within the Heat Shock Protein 70 molecule that modulate cytokine production and maturation of dendritic cells. The Journal of Immunology 174: 3306-3316.
56. Panjwani NN, Popova L, Srivastava PK, (2002) Heat shock proteins gp96 and Hsp70 activate the release of nitric oxide by APCs. The Journal of Immunology 168: 2997-3003.
57. Binder RJ, Blachere NE, Srivastava PK, (2001) Heat shock protein-chaperoned peptides but not free peptides introduced into the cytosol are presented efficiently by Major Histocompatibility Complex I molecules. The Journal of Biological Chemistry 276: 17163-17171.
58. Binder RJ, Srivastava PK, (2005) Peptides chaperoned by heat-shock proteins are a necessary and sufficient source of antigen in the cross-priming of CD8+ T cells. Nature Immunology 6: 593-599.
59. Shang L, Tomasi TB, (2006) The heat shock protein 90-CDC37 chaperone complex is required for signaling by Types I and II interferons. The Journal of Biological Chemistry 281: 1876-1884.
60. Bishop SC, Burlison JA, Blagg BSJ, (2007) Hsp90: A novel target for the disruption of multiple signaling cascades. Current Cancer Drug Targets 7: 369-388.
61. Pratt WB, Toft DO, (2003) Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery. Experimental Biology and Medicine 228: 111-133.
62. Arthur JC, Lich JD, Aziz RK, Kotb M, Ting JP-Y, (2007) Heat shock protein 90 associates with monarch-1 and regulates its ability to promote degradation of NF-κB-inducing kinase. The Journal of Immunology 179: 6291-6296.
63. Byrd CA, Bornmann W, Erdjument-Bromage H, Tempst P, Pavletich N, et al. (1999) Heat shock protein 90 mediates macrophage activation by Taxol and bacterial lipopolysaccharide. Proceedings of the National Academy of Sciences USA 96: 5645-5650.
64. Hsu HY, Wu HL, Tan SK, Li VP, Wang WT, et al. (2007) Geldanamycin interferes with the 90-kDa heat shock protein, affecting lipopolysaccharide-mediated interleukin-1 expression and apoptosis within macrophages. Molecular Pharmacology 71: 344-356.
65. el Mezayen R, el Gazzar M, Seeds MC, McCall CE, Dreskin SC, et al. (2007) Endogenous signals released from necrotic cells augment inflammatory responses to bacterial endotoxin. Immunology Letters 111: 36-44.
66. Asea A, Kraeft S-K, Kurt-Jones EA, Stevenson MA, Chen LB, et al. (2000) HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and a cytokine. Nature Medicine 6: 435-442.
67. Saba JA, McComb ME, Potts DL, Costello CE, Amar S, (2007) Proteomic mapping of stimulus-specific signaling pathways involved in THP-1 cells exposed to Porphyromonas gingivalis or its purified components. Journal of Proteome Research 6: 2211-2221.
68. Jonsson CA, Carlsten H, (2002) Mycophenolic acid inhibits inosine 5′-monophosphate dehydrogenase and suppresses production of pro-inflammatory cytokines, nitric oxide, and LDH in macrophages. Cellular Immunology 216: 93-101.
69. Meshkini A, Yazdanparast R, (2008) Involvement of ERK/MAPK pathway in megakaryocytic differentiation of K562 cells induced by 3-hydogenkwadaphnin. Toxicology in Vitro 22: 1503-1510.
70. Shui H, Gao P, Si X, Ding G (2009) Mycophenolic acid inhibits albumin-induced MCP-1 expression in renal tubular epithelial cells through the p38 MAPK pathway. Molecular Biology Reports epublication ahead of print.
71. Dayton JS, Lindsten T, Thompson CB, Mitchell BS, (1994) Effects of human T lymphocyte activation on inosine monophosphate dehydrogenase expression. Journal of Immunology 152: 984-991.
72. Daxecker H, Raab M, Müller MM, (2002) Influence of mycophenolic acid on inosine 5′-monophosphate dehydrogenase activity in human peripheral blood mononuclear cells. Clinica Chimica Acta 318: 71-77.
73. Oshizawa T, Yamaguchi T, Suzuki K, Yamamoto Y, Hayakawa T, (2003) Possible involvement of optimally phosphorylated L-plastin in activation of superoxide-generating NADPH oxidase. Journal of Biochemistry 134: 827-834.
74. Delanote V, Vandekerckhove J, Gettemans J, (2005) Plastins: versatile modulators of actin organization in (patho)physiological cellular processes. Acta Pharmacologica Sinica 26: 769-779.
75. Liu F, Shinomiya H, Kirikae T, Hirata H, Asano Y, (2004) Characterization of murine grancalcin specifically expressed in leukocytes and its possible role in host defense against bacterial infection. Bioscience, Biotechnology, and Biochemistry 68: 894-902.
76. Mainiero F, Soriani A, Strippoli R, Jacobelli J, Gismondi A, et al. (2000) RAC1/P38 MAPK signaling pathway controls β1 integrin-induced interleukin-8 production in human natural killer cells. Immunity 12: 7-16.
77. Bertini R, Howard OMZ, Dong H-F, Oppenheim JJ, Bizzarri C, et al. (1999) Thioredoxin, a redox enzyme released in infection and inflammation, is a unique chemoattractant for neutrophils, monocytes, and T cells. The Journal of Experimental Medicine 189: 1783-1789.
78. Go Y-M, Halvey PJ, Hansen JM, Reed M, Pohl J, et al. (2007) Reactive aldehyde modification of thioredoxin-1 activates early steps of inflammation and cell adhesion. The American Journal of Pathology 171: 1670-1681.
79. Williams CH Jr, (2000) Thioredoxin-thioredoxin reductase- a system that has come of age. European Journal of Biochemistry 267: 6101.
80. Saitoh M, Nishitoh H, Fujii M, Takeda K, Toiume K, et al. (1998) Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating, kinase (ASK)1. The EMBO Journal 17: 2596-2606.
81. Hirota K, Murata M, Sachi Y, Nakamura H, Takeuchi J, et al. (1999) Distinct roles of thioredoxin in the cytoplasm and in the nucleus. The Journal of Biological Chemistry 274: 27891-27897.
82. Li ZH, Bresnick AR, (2006) The S100A4 metastasis factor regulates cellular motility via a direct interaction with myosin-IIA. Cancer Research 66: 5173-5180.
83. Dukhanina EA, Lukyanova TI, Romanova EA, Dukhanin AS, Sashchenko LP, (2008) Comparative analysis of secretion of S100A4 metastatic marker by immune and tumor cells. Bulletin of Experimental Biology and Medicine 145: 78-80.
84. Tarabykina S, Griffiths TRL, Tulchinsky E, Mellon JK, Bronstein IB, et al. (2007) Metastasis-associated protein S100A4: spotlight on its role in cell migration. Current Cancer Drug Targets 7: 217-228.
85. Zimmer DB, Sadosky PW, Weber DJ, (2003) Molecular mechanisms of S100-target protein interactions. Microscopy Research and Technique 60: 552-559.
86. Semov A, Moreno MJ, Onichtchenko A, Abulrob A, Ball M, et al. (2005) Metastasis-associated protein S100A4 induces angiogenesis through interaction with Annexin II and accelerated plasmin formation. The Journal of Biological Chemistry 280: 20833-20841.
87. Cui JF, Liu YK, Zhang LJ, Shen HL, Song HY, et al. (2006) Identification of metastasis candidate proteins among HCC cell lines by comparative proteome and biological function analysis of S100A4 in metastasis in vitro. Proteomics 6: 5953-5961.
88. Emberly ED, Murphy LC, Watson PH, (2004) S100 proteins and their influence on pro-survival pathways in cancer. Biochemistry and Cell Biology 82: 508-515.
89. Kriajevska M, Fischer-Larsen M, Moertz E, Vorm O, Tulchinsky E, et al. (2002) Liprin β1, a member of the family of LAR transmembane tyrosine phosphatase-interacting proteins, is a new target for the metatasis-associated protein S100A4 (Mts1). The Journal of Biological Chemistry 277: 5229-5235.
90. Rivard CJ, Brown LM, Aleida NE, Maunsbach AB, Pihakaski-Maunsbach K, et al. (2007) Expression of the calcium binding protein S100A4 is markedly upregulated by osmotic stress and is involved in the renal osmoadaptive response. The Journal of Biological Chemistry 282: 6644-6652.
91. Hofmeister-Mueller V, Vetter-Kauczok CS, Ullrich R, Meder K, Lukanidin E, et al. (2009) Immunogenicity of HLA-A1-restricted peptides derived from S100A4 (metastasin 1) in melanoma patients. Cancer Immunology and Immunotherapy 58: 1265-1273.
92. Yasumatsu R, Altiok O, Benarafa C, Yasumatsu C, Bingol-Karakoc G, et al. (2006) SerpinB1 upregulation is associated with in vivo complex formation of neutrophil elastase and cathepsin G in a baboon model of bronchopulmonary dysplasia. American Journal of Physiology Lung, Cellular and Molecular Physiology 291: L619-627.
93. Zeng W, Remold-O'Donnell E, (2000) Human monocyte/neutrophil elastase inhibitor (MNEI) is regulated by PU.1/Spi1, Sp1, and NF-kB. Journal of Cellular Biochemistry 78: 519-532.
94. Benarafa C, Priebe GP, Remold-O'Donnell E, (2007) The neutrophil serine protease inhibitor serpinb1 preserves lung defense functions in Pseudomonas aeruginosa infection. The Journal of Experimental Medicine 204: 1901-1909.
95. Cooley J, Takayama TK, Shapiro SD, Schechter NM, Remold-O'Donnell E, (2001) The serpin MNEI inhibits elastase-like and chymotrypsin-like serine proteases through efficient reactions at two active sites. Biochemistry 40: 15762-15770.
96. Izuhara K, Ohta S, Kanaji S, Shiraishi H, Arima K, (2008) Recent progress in understanding the diversity of the human ov-serpin/clade B serpin family. Cellular and Molecular Life Science 65: 2541-2553.
97. Tseng M-Y, Liu SY, Chen H-R, Wu Y-J, Chiu C-C, et al. (2009) Serine protease inhibitor (Serpin) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma. Oral Oncology: epublication ahead of print.
98. Woods DE, Cantin A, Cooley J, Kenney DM, Remold-O'Donnell E, (2005) Aerosol treatment with MNEI suppresses bacterial proliferation in a model of chronic Pseudomonas aeruginosa lung infection. Pediatric Pulmonology 39: 141-149.
99. Rana S, Gupta K, Gomez J, Matsuyama S, Chakrabarti A, et al. (2010) Securinine induces p73-dependent apoptosis preferentially in p53-deficient colon cancer cells. The FASEB Journal 24: 2126-2134.
100. Howe D, Heinzen RA, (2005) Replication of Coxiella burnetii is inhibited in CHO K-1 cells treated with inhibitors of cholesterol metabolism. Annals of the NY Academy of Sciences 1063: 123-129.
101. Howe D, Heinzen RA, (2006) Coxiella burnetii inhabits a cholesterol-rich vacuole and influences cellular cholesterol metabolism. Cellular Microbiology 8: 496-507.
102. Dang PM, Stensballe A, Boussetta T, Raad H, Dewas C, et al. (2006) A specific p47phox-serine phosphorylated by convergent MAPKs mediates neutrophil NADPH oxidase priming at inflammatory sites. The Journal of Clinical Investigation 116: 2033-2043.
103. Lukashova V, Asselin C, Krolewski JJ, Rola-Pleszczynski M, Staňková J, (2001) G protein independent activation of Tyk2 by the Platelet-Activating Factor receptor. The Journal of Biological Chemistry 276: 24113-24121.
104. Pedersen SK, Harry JL, Sebastian L, Baker J, Traini MD, et al. (2003) Unseen proteome: mining below the tip of the iceberg to find low abundance and membrane proteins. Journal of Proteome Research 2: 303-311.
105. Görg A (1998) Two-dimensional electrophoresis of proteins using immobilized pH gradients: A laboratory manual. Available: http://patf.lf1.cuni.cz/proteomika/2d-manual.pdf. Accessed 2012 Jul 1.
106. Candiano G, Bruschi M, Musante L, Santucci L, Ghiggeri GM, et al. (2004) Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25: 1327-1333.