Site-directed mutagenesis of a tetrameric dandelion polyphenol oxidase (PPO-6) reveals the site of subunit interaction

Plant Molecular Biology

Volumn 80, Issue 2, 2012, Pages 203-217

  Dirks Hofmeister, Mareike E ^a   Inlow, Jennifer K ^b   Moerschbacher, Bruno M ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b INDIANA STATE UNIVERSITY   (United States)

Author keywords

Cooperativity; Enzyme structure; Molecular modeling; Plant defense; Polyphenol oxidase; Site directed mutagenesis; Taraxacum officinale

Indexed keywords

CATECHOL OXIDASE; CYSTEINE; MUTANT PROTEIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; BIOCATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; DANDELION; ENZYME STABILITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN MULTIMERIZATION; PROTEIN SUBUNIT; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITES; BIOCATALYSIS; CATECHOL OXIDASE; CYSTEINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PLANT PROTEINS; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN SUBUNITS; TARAXACUM;

ESCHERICHIA COLI; TARAXACUM OFFICINALE;

EID: 84865435097     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-012-9943-9     Document Type: Article

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