Acid activation of protyrosinase from Aspergillus oryzae: Homo-tetrameric protyrosinase is converted to active dimers with an essential intersubunit disulfide bond at acidic pH

Pigment Cell and Melanoma Research

Volumn 21, Issue 1, 2008, Pages 89-96

  Tatara, Yota ^a,b   Namba, Takeshi ^a   Yamagata, Youhei ^b   Yoshida, Takashi ^c   Uchida, Takafumi ^b   Ichishima, Eiji ^a,b  

^a SOKA UNIVERSITY   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

^c HIROSAKI UNIVERSITY   (Japan)

Author keywords

Acid activation; Conformational change; Intermolecular disulfide bond; Polar interaction; Quaternary structure; Tyrosinase

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; AMINO ACID DERIVATIVE; CYSTEINE; DIMER; DODECYL SULFATE SODIUM; MONOPHENOL MONOOXYGENASE; PROTYROSINASE; UNCLASSIFIED DRUG;

ARTICLE; ASPERGILLUS ORYZAE; BINDING ASSAY; CIRCULAR DICHROISM; CONTROLLED STUDY; DIMERIZATION; DISULFIDE BOND; ENZYME STRUCTURE; GEL FILTRATION CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PH MEASUREMENT; PROTEIN FUNCTION;

ANILINO NAPHTHALENESULFONATES; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; CYSTEINE; DIMERIZATION; DISULFIDES; ENZYME ACTIVATION; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; HYDROPHOBICITY; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN CONFORMATION; PROTEIN PRECURSORS; RECOMBINANT PROTEINS; SODIUM DODECYL SULFATE; SPECTROMETRY, FLUORESCENCE;

ASPERGILLUS ORYZAE;

EID: 41049097350     PISSN: None     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1755-148X.2007.00422.x     Document Type: Article

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